References of "LE GOFF, Caroline"
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See detailLe Stress Oxydant: arnaque ou biologie d'avenir?
LE GOFF, Caroline ULg

Conference (2017, September 28)

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See detailA fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS
Fabregat Cabello, Neus ULg; Farre Segura, Jordi ULg; Huyghebaert, Loreen ULg et al

in Clinica Chimica Acta (2017)

BACKGROUND: Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have ... [more ▼]

BACKGROUND: Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. METHODS: Sample preparation was based on precipitation and centrifugation of 100 µL of patient serum, followed by injection into the LC-MS/MS system. Samples from Vitamin D Standardization Program (n=80) and patient samples (n=281) have been compared with a reference LC-MS/MS method. For the analytical validation NIST and Labquality quality control materials were used. RESULTS: Mean intra-assay and inter-assay imprecision were less than 6.0 and 6.4% and mean recoveries were within 95-104%. LOQ’s were 0.5 µg/L for 24,25(OH)2D3, 1.1 µg/L for 25(OH)D3 and epi-25(OH)D3 and 1.7 µg/L for 25(OH)D2. A 3% bias obtained between the proposed and the reference method satisfies Vitamin D Standardization Program recommendations. CONCLUSIONS: We present a rapid, easy, reliable and cost-effective method completely adequate for routine testing, which permits the measurement of the ratio of 24,25-dihydroxyvitamin D to 25-hydroxyvitamin D, Vitamin D Metabolite Ratio (VMR), in serum samples. [less ▲]

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See detailReference interval determination for serum and urine aldosterone for healthy Belgian population
LE GOFF, Caroline ULg; Fabregat Cabello, Neus ULg; Huyghebaert, Loreen ULg et al

in Clinical Chemistry & Laboratory Medicine (2017, June)

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See detailEstablishment of reference values for 6 six steroids in serum
LE GOFF, Caroline ULg; Fabregat Cabello, Neus ULg; Huyghebaert, Loreen ULg et al

in Clinical Chemistry and Laboratory Medicine (2017, June)

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See detailComparison of cardiac biomarker fluctuation in runners of marathons, semi-marathons and untrained runners
LE GOFF, Caroline ULg; VRANKEN, Laura ULg; van Nueten, Jan et al

in Clinical Chemistry & Laboratory Medicine (2017, June)

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See detailQuantification of serum androstanediol glucuronide
LE GOFF, Caroline ULg

in Clinical Chemistry and Laboratory Medicine (2017, June)

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See detailImpact of an ultra-trail of 330 km on plasma levels
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Gergelé, Laurent et al

in Clinical Chemistry and laboratory medicine (2017, June)

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See detailEccentric training for tendon healing after lesion: a rat model
Kaux, Jean-François ULg; Libertiaux, Vincent ULg; Leprince, Pierre ULg et al

in American Journal of Sports Medicine (2017), 45(6), 1440-1446

BACKGROUND:The tendon is a dynamic entity that remodels permanently. Platelet-rich plasma (PRP) injection has been shown to have a beneficial effect on tendon healing after lesion in rats. Furthermore ... [more ▼]

BACKGROUND:The tendon is a dynamic entity that remodels permanently. Platelet-rich plasma (PRP) injection has been shown to have a beneficial effect on tendon healing after lesion in rats. Furthermore, eccentric exercise seems to improve the mechanical quality of the tendon. HYPOTHESIS:A combination of PRP injection and eccentric training might be more effective than either treatment alone. STUDY DESIGN:Controlled laboratory study. METHODS:Adult male rats were anesthetized, an incision was performed in the middle of their left patellar tendon and an injection of physiological fluid (PF) or homologous PRP was randomly made at the lesion level. The rats were then divided into 2 groups: the eccentric group, undergoing eccentric training 3 times a week, and the untrained group, without any training. Thus, 4 groups were compared. After 5 weeks, the tendons were removed and their ultimate tensile strength and energy were measured. Tendons were frozen for proteomic analyses when all biomechanical tests were completed. Statistical analysis was performed with linear mixed effect models. RESULTS:No significant difference was found between the treatments using PF injection or PRP injection alone. However, the value of the ultimate tensile force at rupture was increased by 4.5 N (108% of control, P = .006) when eccentric training was performed. An intragroup analysis revealed that eccentric training significantly improved the ultimate force values for the PRP group. Proteomic analysis revealed that eccentric training led to an increase in abundance of several cytoskeletal proteins in the PF group, while a decrease in abundance of enzymes of the glycolytic pathway occurred in the PRP treated groups, indicating that this treatment might redirect the exercise-driven metabolic plasticity of the tendon. CONCLUSION: Eccentric training altered the metabolic plasticity of tendon and led to an improvement of injured tendon resistance regardless of the treatment injected (PF or PRP). CLINICAL RELEVANCE:This study demonstrates the necessity of eccentric rehabilitation and training in cases of tendon lesion regardless of the treatment carried out. [less ▲]

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See detailAnalytical and clinical validation of the new Abbot Architect 25(OH)D assay: fit for purpose?
Cavalier, Etienne ULg; LUKAS, Pierre ULg; BEKAERT, Anne-Catherine ULg et al

in Clinical Chemistry & Laboratory Medicine (2017), 55(3), 378-384

BACKGROUND: We provide a clinical and analytical evaluation of the reformulated version of the Abbott Architect 25-hydroxyvitamin D assay. We compared this assay with three commercial automated ... [more ▼]

BACKGROUND: We provide a clinical and analytical evaluation of the reformulated version of the Abbott Architect 25-hydroxyvitamin D assay. We compared this assay with three commercial automated immunoassays and against a VDSP-traceable liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in six different populations. We also supplemented 40 healthy volunteers with either 600,000 IU of vitamin D2 or 100,000 of vitamin D3 to evaluate the performance of the immunoassays vs. the LC-MS/MS. METHODS: Precision and limit of quantification were assessed, 25(OH)D2 and C3-epimer recovery were calculated. Two hundred and forty samples obtained in healthy Caucasians and Africans, osteoporotic, hemodialyzed and intensive care patients and 3rd trimester pregnant women were analyzed by all methods. Correlation was studied using Passing-Bablok and Bland-Altman analysis. Concordance correlation coefficient (CCC) was calculated to evaluate agreement between immunoassays and LC-MS/MS. We verified if patients were homogeneously classified with the immunoassays when they took vitamin D2 or vitamin D3 after 1, 7 and 28 days. RESULTS: We observed excellent analytical features and showed a very good correlation to the LC-MS/MS results in the overall population. Compared to the other immunoassays, concordance of the new Abbott assay with the LC-MS/MS was at least similar, and often better in diseased populations. Althought the cross-reactivity with 25(OH)D2 was not of 100%, there was no significant difference in the classifications of the patients, either supplemented with D2 or D3 or after 7 or 28 days. CONCLUSIONS: This modified version of the Abbott Architect assay is clearly improved compared to the previous one and presents a better agreement with the LC-MS/MS. [less ▲]

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See detailImpact of an ultra-marathon of 330km on plasma levels of cardiac biomarkers
Le Goff, Caroline ULg; Kaux, Jean-François ULg; Gergelé, Laurent et al

in British Journal of Sports Medicine (2017, February), 51(4), 347

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See detailLe ST2: nouveau marqueur de fibrose
LE GOFF, Caroline ULg

Scientific conference (2016, December 15)

Review sur le ST2, un nouveau marqueur de fibrose cardiaque

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See detailAre there biologic factors of good prognosis after a platelet rich plasma (PRP) injection for tendinopathies ?
Libertiaux, Vincent ULg; Sancerne, Audrey; LE GOFF, Caroline ULg et al

in Journal of Exercise, Sports & Orthopedics (2016), 3(2), 1-4

Background : The aims of this study were to evaluate the effect of a single PRP injection followed by a standardized reeducation protocol among patients suffering from different tendinopathies, but ... [more ▼]

Background : The aims of this study were to evaluate the effect of a single PRP injection followed by a standardized reeducation protocol among patients suffering from different tendinopathies, but otherwise healthy, and to determine the biomarkers that constitute good prognosis factors, if any. Methods : 48 patients suffering from different tendinopathies and refractory to conventional physiotherapy were treated with a single PRP injection. Prior to the injection, a blood sample was drawn and some biological parameters (glycemia, cholesterolemia,...) were measured. A pain assessment was then made using a Visual Analog Scale of pain (VAS) and a pressure algometer. The same assessment was carried out after 6 weeks and 12 weeks when possible. Results : There is an overall significant improvement in VAS score at the end of the 12 weeks follow- up. However, no correlation was found between the evolution of the clinical scores and the biological parameters measured. Conclusion: A PRP injection followed by a program of eccentric rehabilitation positively affects the algo-functional scores of patients with tendinopathies who were refractory to conventional physio- therapy, whatever the initial values of the measuured biological parameters. [less ▲]

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See detailMyoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer
Blomme, Arnaud; Costanza, Brunella ULg; De Tullio, Pascal ULg et al

in Oncogene (2016)

Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as ... [more ▼]

Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as overexpressed in several cancers and shown to contribute to proliferation, migration and invasion of cancer cells. We have previously demonstrated that myoferlin regulates epidermal growth factor receptor activity in breast cancer. In the current study, we report a consistent overexpression of myoferlin in triple-negative breast cancer cells (TNBC) over cells originating from other breast cancer subtypes. Using a combination of proteomics, metabolomics and electron microscopy, we demonstrate that myoferlin depletion results in marked alteration of endosomal system and metabolism. Mechanistically, myoferlin depletion caused impaired vesicle traffic that led to a misbalance of saturated/unsaturated fatty acids. This provoked mitochondrial dysfunction in TNBC cells. As a consequence of the major metabolic stress, TNBC cells rapidly triggered AMP activated protein kinase-mediated metabolic reprogramming to glycolysis. This reduced their ability to balance between oxidative phosphorylation and glycolysis, rendering TNBC cells metabolically inflexible, and more sensitive to metabolic drug targeting in vitro. In line with this, our in vivo findings demonstrated a significantly reduced capacity of myoferlin-deficient TNBC cells to metastasise to lungs. The significance of this observation was further supported by clinical data, showing that TNBC patients whose tumors overexpress myoferlin have worst distant metastasis-free and overall survivals. This novel insight into myoferlin function establishes an important link between vesicle traffic, cancer metabolism and progression, offering new diagnostic and therapeutic concepts to develop treatments for TNBC patients. [less ▲]

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See detailNovel cardiac markers and subsequent new recommendations
LE GOFF, Caroline ULg; D'ORIO, Virginie ULg

Scientific conference (2016, October 14)

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See detailLes nouveaux marqueurs cardiaques
LE GOFF, Caroline ULg

in Résumé du congrès CORATA (2016)

De nombreux nouveaux biomarqueurs ont vu le jour...mais lesquels sont utilles? Voici une revue de la littérature.

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See detailQuantification of serum androstanediol glucuronide
LE GOFF, Caroline ULg; Fabregat Cabello, Neus ULg; Cavalier, Etienne ULg

Scientific conference (2016, September 14)

Validation of the method by LCMSMS

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