References of "Kinet-Denoel, C"
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See detailRadioresistance of follicular dendritic cells
Mancini, I.; Kinet-Denoël, C.; Brait, M. et al

in Follicular dendritic cells in normal and pathological conditions (1995)

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See detailThe germinal centre: a monastery or a bar?
Heinen, Ernst ULg; Tsunoda, T.; Marcoty, C. et al

in Research in Immunology (1991), 142(3), 242-4

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See detailAre germinal centers insulating microenvironments?
Heinen, Ernst ULg; Tsunoda, R.; Bosseloir, A. et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailUltrastructural and functional aspects of FDC in vitro
Heinen, Ernst ULg; Cormann, N.; Tsunoda, R. et al

in Accessory cells in HIV and other retroviral infections (1991)

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See detailIntercellular communications in lymph follicles
Heinen, Ernst ULg; Tsunoda, R.; Marcoty, C. et al

in Dendritic cells in lymphoid tissues (1991)

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See detailPurification and cultivation of follicular dendritic cells.
Marcoty, C.; Heinen, Ernst ULg; Bosseloir, A. et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailIL6 and IL4: localization and production in human tonsils
Bosseloir, A.; Hooghe-Peters, E.; Heinen, Ernst ULg et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailIsolation and long-term cultivation of human tonsil follicular dendritic cells.
Tsunoda, R.; Nakayama, M.; Onozaki, K. et al

in Virchows Archiv. B : Cell pathology (1990), 59(2), 95-105

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of ... [more ▼]

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of FDC and germinal center lymphoid cells, useful for the analysis of the relationship between these two cell types and of the behavior of FDC in culture. During the first few days of culture, lymphoid cells located between FDC extensions survived better than those which were free or partly covered by FDC. After 6 days, the lymphoid population degenerated and only the FDC survived. The unique antigenic pattern of FDC (positive for HLA-DR. DRC-1, CD14b, CD21, CD23, CD35) disappeared within a few days of culture. Recombinant interferon-gamma exerted a positive effect either on retaining HLA-DR expression or on the reexpression of these antigens by FDC. HLA-ABC antigens were traced until the 10th day and desmosomal junctions until the 14th day. Subsequently, FDC presented peculiar features, including oval and rhomboid shapes, one to ten nuclei, fine amoeboid extensions, stress fibers and a radical dense zone in their cytoplasm. FDC possessed actin, tubulin and vimentin, but neither desmin nor cytokeratin. After 40 days of culture, FDC enlarged and were covered with abundant membrane extensions. Even when kept as long as 150 days in vitro. FDC did not proliferate in any of the culture conditions employed. [less ▲]

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See detailMicroenvironments during antigen stimulation
Heinen, Ernst ULg; Bosseloir, A.; Cormann, N. et al

in Molecular biology of B cells developements (1990)

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See detailIntercellular Contacts between Germinal Center Cells. Mechanisms of Adhesion between Lymphoid Cells and Follicular Dendritic Cells
Louis, Edouard ULg; Philippet, B.; Cardos, B. et al

in Acta Oto-Rhino-Laryngologica Belgica (1989), 43(4), 297-320

Intercellular connections exist between germinal center cells especially between lymphoid cells and follicular dendritic cells (FDC). Even after isolation, FDC remain associated to lymphocytes and are ... [more ▼]

Intercellular connections exist between germinal center cells especially between lymphoid cells and follicular dendritic cells (FDC). Even after isolation, FDC remain associated to lymphocytes and are able, in a cell suspension, to establish new connections with others. Using human tonsillar cells or mouse lymph node cells we analysed these connections which were shown to be species-specific. Low temperature as well as absence of Ca++ and Mg++ in the culture medium reduced the adherence of fluorochrome-labeled lymphoid cells to FDC. Colchicine treatment did not impair the adherence, whereas cytochalasin B dit it; this was the first observation underlining the importance of microfibrils in FDC. Antibodies directed towards integrin molecules (LFA-1 alpha or beta chain, CD11a and CD18 respectively) reduced the adherence, others (anti-CR3 or anti-gp 150/95, CD11b and c respectively) did not influence it. Antibodies directed against MHC class II exerted no inhibitory action on the lymphoid cell adhesion to FDC. As, at ultrastructural level, gold-labeled immune complexes can be found between FDC and lymphoid cells, we examined the effect on cell adhesion of the addition of immune complexes to the cell suspensions. It only impaired the lymphoid cell adhesion when complement components were present. IgM complexes were then more inhibitory than IgG complexes. When antibodies against Fc IgG receptors (CD16) were added, the adhesion was strongly reduced whereas antibodies to Fc IgE (CD23) receptors had no influence. The antibody DRC1, specifically recognizing an antigen on human FDC reduced the attachment of cells to FDC. This antigen thus seems to play a role in the intercellular contacts; this is the first function ascribed to this FDC specific antigen. [less ▲]

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See detailLocalization of interleukin 6 mRNA in human tonsils by in situ hybridization.
Bosseloir, A.; Hooghe-Peters, E. L.; Heinen, Ernst ULg et al

in European Journal of Immunology (1989), 19(12), 2379-81

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 ... [more ▼]

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 cDNA we demonstrated IL 6 gene expression over various areas of the tonsils, with consistent exception of the follicles, by in situ hybridization. It is, therefore, proposed that B cells are stimulated during their migration out of the follicles. [less ▲]

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See detailCytokines produced in lymph follicles.
Tsunoda, R.; Cormann, N.; Heinen, Ernst ULg et al

in Immunology Letters (1989), 22(2), 129-34

The events occurring inside lymph follicles during a germinal center reaction are poorly understood. Using B and T lymphoid cell populations prepared from human tonsillar lymph follicles, and enriched or ... [more ▼]

The events occurring inside lymph follicles during a germinal center reaction are poorly understood. Using B and T lymphoid cell populations prepared from human tonsillar lymph follicles, and enriched or not in macrophages or in follicular dendritic cells, we examined the production of cytokines by these cells in vitro. Interleukin 6 (IL-6) and tumor necrosis factor (TNF) were found in the supernatants of cultures stimulated with phytohemagglutinin or pokeweed mitogen. IL-1 beta was occasionally detected; its secretion apparently depends on the origin of the tonsils, the stimulation, and the cell populations. IFN-gamma and IL-2 were not produced in significant amounts by these lymph follicle cells. IL-4 was only found in very low concentrations in the supernatant of the different cell cultures. The cell populations containing follicular dendritic cells produced more IL-6 and TNF than the others, especially than those composed of only B and T cells. [less ▲]

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See detailEtude de la proliferation lymphocytaire humaine in vitro en reponse a l'anatoxine tetanique.
Lecart, Marie-Paule ULg; Cormann, N.; Heinen, Ernst ULg et al

in Revue Médicale de Liège (1989), 44(13-14), 455-63

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See detailImmunohistochemical study on cultured FDC-C enriched lymphoid cell populations.
Tsunoda, R.; Cormann, N.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1988), 237

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See detailIsolation of follicular dendritic cells from human tonsils and adenoids. In vitro culture.
Cormann, N.; Heinen, Ernst ULg; Kinet-Denoel, C. et al

in Advances in Experimental Medicine and Biology (1988), 237

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See detailInteractions between follicular dendritic cells and lymphoid cells.
Heinen, Ernst ULg; Braun, M.; Louis, Edouard ULg et al

in Advances in Experimental Medicine and Biology (1988), 237

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See detailInfluence of immunoglobulin isotypes and lymphoid cell phenotype on the transfer of immune complexes to follicular dendritic cells.
Braun, M.; Heinen, Ernst ULg; Cormann, N. et al

in Cellular Immunology (1987), 107(1), 99-106

Follicular dendritic cells (FDC) are located only inside lymph follicles and are characterized mainly by their capacity to retain high amounts of immune complexes by their Fc or C3b receptors. In this ... [more ▼]

Follicular dendritic cells (FDC) are located only inside lymph follicles and are characterized mainly by their capacity to retain high amounts of immune complexes by their Fc or C3b receptors. In this work, we examine the influence of immunoglobulin isotypes and the subset of lymphoid cells (B or T) upon the transfer of immune complexes from lymphocytes to FDC. FDC isolated from mice lymph nodes by enzymatic digestion are able to fix, through Fc receptors, gold-labeled immune complexes presented by lymphoid cells. As demonstrated by electron microscopy, this transfer requires the establishment of close contacts between both cell types. Using different cell selection techniques we show that B lymphoid cells take up immune complexes more efficiently than do T lymphoid cells and transfer a larger number of them to FDC. This transfer mechanism is dependent on the immunoglobulin isotype: immune complexes constituted of IgG2a, IgG2b, and IgG1 isotypes are better transferred to FDC than those constituted of IgG3 and IgM. [less ▲]

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See detailIsolation of follicular dendritic cells from human tonsils and adenoids. V. Effect on lymphocyte proliferation and differentiation.
Cormann, N.; Lesage, F.; Heinen, Ernst ULg et al

in Immunology Letters (1986), 14(1), 29-35

Follicular dendritic cells (FDC) are located only in lymph follicles and are characterized by their capacity to retain high amounts of immune complexes on their plasma membranes. As their functions in ... [more ▼]

Follicular dendritic cells (FDC) are located only in lymph follicles and are characterized by their capacity to retain high amounts of immune complexes on their plasma membranes. As their functions in germinal centres are unknown, we isolated them from human tonsils and cultured them with autologous lymphoid cells. Cultures of lymphoid cells alone or with added macrophages were used as controls. Lymphoid cells incorporated tritiated thymidine only when FDC and lectins were added; this could be shown after several periods of time. However, the Ig secretion by lymphoid cell populations was inhibited by FDC after several days in vitro. In contrast, the supernatants of lymphocytes cultured alone or with macrophages only for the same periods of time contained increasing amounts of immunoglobulins. This inhibitory effect of FDC on immunoglobulin production was observed for all considered isotypes. Our data suggest that FDC stimulate lymphoid cell proliferation but reduce B-cell differentiation. This is the first accessory cell activity definitely shown for FDC in cultures. [less ▲]

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See detailRetention of immune complexes by murine lymph node or spleen follicular dendritic cells. Role of antibody isotype.
Heinen, Ernst ULg; Coulie, P.; Van Snick, J. et al

in Scandinavian Journal of Immunology (1986), 24(3), 327-34

Using monoclonal anti-trinitrophenyl (TNP) antibodies complexed to TNP-myoglobin-coated gold particles, we analysed at the ultrastructural level the retention by follicular dendritic cells (FDC) of immune ... [more ▼]

Using monoclonal anti-trinitrophenyl (TNP) antibodies complexed to TNP-myoglobin-coated gold particles, we analysed at the ultrastructural level the retention by follicular dendritic cells (FDC) of immune complexes containing various antibody isotypes. Gold-labelled immune complexes were injected subcutaneously or intravenously into naive mice and, after 24 h, germinal centres of draining lymph nodes or spleen were examined by electron microscopy. FDC generally retained complexes containing IgG2a and IgG2b better than those formed with IgG1 or IgG3. IgM was rarely retained. FDC isolated from lymph nodes or spleens were incubated in vitro with gold-labelled complexes in a serum-free medium. IgG2a and IgG2b complexes were also retained in vitro in large quantities by FDC; IgG1 and IgG3 complexes were retained in smaller quantities or in highly variable quantities compared with IgG2; IgM complexes were rarely seen on FDC. There was no difference between FDC isolated from lymph nodes or from spleen with respect to the Ig isotypes required for Fc-mediated retention of immune complexes. [less ▲]

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See detailLipopolysaccharide suppresses immune complex retention by follicular dendritic cells without cytological alterations.
Heinen, Ernst ULg; Cormann, N.; Kinet-Denoel, C. et al

in Immunology Letters (1986), 13(6), 323-7

Follicular dendritic cells (FDC) are peculiar cells only located inside lymph follicles and which may be characterized by complex dendritic evaginations retaining high quantities of immune complexes by Fc ... [more ▼]

Follicular dendritic cells (FDC) are peculiar cells only located inside lymph follicles and which may be characterized by complex dendritic evaginations retaining high quantities of immune complexes by Fc and C3b receptors. After lipopolysaccharide (LPS) injection in mice the retention of gold-labelled immune complexes was abolished in draining lymph nodes. In order to examine the possibility that the transport of immune complexes to lymph follicles was impaired, we isolated FDC from lymph nodes and incubated them in presence of gold-labelled complexes: no or strongly reduced retention was then observed at the ultrastructural level. This LPS-induced impairment of immune complex fixation by FDC is not due to morphological alteration to the cells but to the inhibition of their Fc and C3b receptors. Further, LPS induces changes in the composition of the lymphocyte population in lymph follicles as higher numbers of blast cells and plasmocytes are observed after treatment. [less ▲]

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