References of "Kiewisz, J"
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See detailIsolation of pregnancy-associated glycoproteins of the American bison (Bison bison) at first half of pregnancy
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in General and Comparative Endocrinology (2008), 155(1), 164-175

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins ... [more ▼]

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins were purified from foetal cotyledonary tissues (CT) and liquid cotyledonary-carrying proteins (LCP) leaking from damaged cells. Our protocols successfully indicated the usefulness of AmbPAG protein identification, especially from LCP fraction. The AmbPAGs were extracted, precipitated and eluted during DEAE cellulose chromatography. The richest protein fractions were further chromatographed on VVA (Vicia villosa agglutinin affinity column), then characterised by mono- and bi-dimensional electrophoresis, Western blot and N-terminal amino acid (aa) sequence. After being transferred to PVDF membranes, three selected VVA-purified AmbPAG isoforms differing in molecular masses and isoelectric points (Ip 4-4.6) were selected for sequencing. One identified N-terminal 25 aa sequence of AmbPAG72 kDa CT form was identified as completely new (RGSNI_TSLPLQNVIDLFYVGNITIG). Two other AmbPAG proteins purified from different sources (74 kDa CT and 76 kDa LCP forms; RGSNLTIHPLRNIRDIFYVGNITIG) were identical or corresponded to N-terminus of various bovine PAGs (boPAG). The two AmbPAGs (74 kDa CT and 76 kDa LCP) revealed identical micro-sequence to boPAG7; and were similar mainly to bovine PAG4, -6, -15 and -17 precursors that were identified by full-length sequencing derived from cDNA cloning. The novel sequence of the AmbPAG (72 kDa CT) was related to some boPAG and various other ruminant PAG precursors (caprine and ovine). All three identified AmbPAG sequences were also relatively similar to mature forms of purified native boPAG56-75kDa proteins. This is the first report indicating aa sequences of native AmbPAG proteins purified from placenta (CT and LCP) of bison species. The N-terminal sequences of the AmbPAGs have been deposited in the EMBL-EBI database (UniProtKB; Accession Nos.: P84916, P84917 and P84918). (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

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See detailUnknown placental glycoprotein K-76 kDa [Fragment] from Bison bonasus placenta - Access number P85330
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailUnknown placental glycoprotein J2-45 kDa [Fragment] from Bison bonasus placenta - Access number P85329
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailUnknown placental glycoprotein J1-45 kDa [Fragment] from Bison bonasus placenta - P85328
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein I-50 kDa [Fragment] from Bison bonasus placenta - Access number P85327
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein H-60 kDa [Fragment] from Bison bonasus placenta - Access number P85326
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein G-71 kDa [Fragment] from Bison bonasus placenta - Access number P85325
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein F-50 kDa [Fragment] from Bison bonasus placenta - Access number P85324
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein E-55 kDa [Fragment] from Bison bonasus placenta - Access number P85323
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-50 kDa [Fragment] from Bison bonasus placenta - Access number P85322
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-50 kDa [Fragment] from Bison bonasus placenta - Access number P85321
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-55 kDa [Fragment] from Bison bonasus placenta - Access number P85320
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-67 kDa [Fragment] from Bison bonasus placenta - Access number P85319
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailIdentification of multiple pregnancy-associated glycoproteins (PAGs) purified from the European bison (Eb; Bison bonasus L.) placentas.
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Animal Reproduction Science (2008), 112

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were ... [more ▼]

This paper describes the first identified chorionic PAGs in the European bison (Eb), named EbPAGs, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). Many EbPAGs were extracted from various cotyledonary tissues, precipitated, chromatographed (DEAE and VVA: Vicia villosa agglutinin), electrophoresed (1D- and 2D-PAGE), analysed by heterologous (cross-species) Western blotting and then micro-sequenced by Edman degradation. Finally, twelve selected VVA-purified isoforms (Ip 3.7-7.4) were entirely characterised. Nine identified NH(2)-terminal micro-sequences were found to be PAGs. On 45dpc, three identified forms were named: EbPAG(67AkDa) (RGSNLTHPLRNIGDLFYVGN), EbPAG(55BkDa) (RGSNLTHPL) and EbPAG(50CkDa) (SQISLRGSNLTI). On 60dpc, the next three forms were named: EbPAG(71DkDa) (RGSNLTIHPLRNIIDLFYVG), EbPAG(55EkDa) (RGSNLTHPLRNI) and EbPAG(50FkDa) (SQISLRGS). On 120dpc, three other forms were named: EbPAG(71GkDa) (RGSNLTHPLRNIRDLFYVG), EbPAG(60HkDa) (RGSNLTTHPLRNIKDLVVYM) and EbPAG(50IkDa) (SGSNLTTV). These EbPAG ((A-I)) sequences are unique, as they are not identical to any other PAGs purified previously in related species of the Bovidae family. However, the EbPAGs (A-I forms) have some sequence resemblance to internal sequences of various full-length polypeptide PAG precursors (in silico translated from cloned cDNAs) identified in domestic cattle. Three other novel native isoforms (J1, J2 and K): EbUPG(45kDa) J1 (SKDNYKNYIPLIVPFAT), EbUPG(45kDa) J2 (SKDNQKNYIPLIVPFAT) and EbUPG(76kDa) K (SPEFTV), were temporarily named 'unknown placental glycoproteins' (UPGs), due to their efficient VVA-purification (specific for glycoproteins only) and a lack of considerable consensus to previously sequenced placental glycoproteins in the Bovidae family. This is the first study identifying NH(2)-terminals of multiple/diverse EbPAGs and some EbUPGs purified from the synepitheliochorial cotyledonary placenta of the endangered Bison bonasus (Red list) [less ▲]

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See detailPregnancy-Associated Glycoprotein 76 [Fragment] from Bison bison placenta - Access number P84918
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2006)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bison. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 74 [Fragment] from Bison bison placenta - Access number P84917
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg

E-print/Working paper (2006)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bison. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 72 [Fragment] from Bison bison placenta - Access number P84916
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2006)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bison. Proteins were submitted to SwissProt databank.

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