  The combined bone forming capacity of human periosteal derived cells and calcium phosphates.; Geris, Liesbet  ; et alin Biomaterials (2011), 32(19), 4393-405 Current knowledge suggests that the periosteum, a fibrous tissue which covers the surface of all bones, contains a population of progenitor cells which mediate the repair of bone defects. In an effort to ... [more ▼] Current knowledge suggests that the periosteum, a fibrous tissue which covers the surface of all bones, contains a population of progenitor cells which mediate the repair of bone defects. In an effort to optimise the utilisation of this source of cells for bone engineering, herein we describe the rational selection of calcium phosphate (CaP) containing materials, based on biomaterial properties, and evaluation of their combined bone forming capacity. Five different commercially available orthopaedic 3D matrices composed of CaP particles in an open collagen network (NuOss, CopiOs, Bio-Oss((R)), Collagraft and Vitoss((R))) were evaluated in vitro and in vivo with human periosteal derived cells (hPDCs). It was found that the cell-material combinations behaved quite differently in vivo, despite apparent in vitro similarities in gene expression profiles. Bone formation was highest within the NuOss/hPDC implant at 13.03%, which also contained the highest incidence of bone marrow formation. The bone formed in this implant was chimeric with approximately 65% originating from implanted cells. Upon analysis of human specific gene expression, although it was found that predominantly osteogenic differentiation was observed within NuOss/hPDC implants, a lesser induction of chondrogenic genes was also observed. The formation of a cartilage intermediate was confirmed by histology. Additionally the NuOss/hPDC implant integrated into the mouse environment with apparent active scaffold resorption. This study demonstrates the importance of matching a cell support/biological matrix with a cell type and subsequently has outlined parameters which can be used for the rational selection of biomaterials for bone engineering. [less ▲] Detailed reference viewed: 15 (0 ULg) Characterization of the porous structure of biodegradable scaffolds obtained with supercritical CO2 as foaming agentLéonard, Angélique ; Calberg, Cédric ; et alin Journal of Porous Materials (2008), 15(4), 397-403 Poly(ε-caprolactone) foams were prepared, via a batch process, by using supercritical CO2 as foaming agent. Their porous structure was characterized through mercury porosimetry, helium and mercury ... [more ▼] Poly(ε-caprolactone) foams were prepared, via a batch process, by using supercritical CO2 as foaming agent. Their porous structure was characterized through mercury porosimetry, helium and mercury pycnometry, scanning electron microscopy (SEM) and X-ray microtomography observations coupled with image analysis. The pore size distributions obtained by these two latter techniques show that the pore structure is more homogeneous when the foaming process is performed under a high CO2 saturation pressure (higher than 250 bars). [less ▲] Detailed reference viewed: 93 (30 ULg)The porous structure of biodegradable scaffolds obtained with supercritical CO2 as foaming agentBlacher, Silvia ; Calberg, Cédric ; et alin Studies in Surface Science and Catalysis (2006), 160 Detailed reference viewed: 7 (2 ULg)X-ray microtomography coupled with image analysis: a useful tool or 3D characterization of polymer foamsBlacher, Silvia ; ; Calberg, Cédric et alConference (2005, October) Detailed reference viewed: 34 (4 ULg)The porous structure of biodegradable scaffolds obtained with supercritical CO2 as foaming agentBlacher, Silvia ; Calberg, Cédric ; et alPoster (2005, May) Detailed reference viewed: 4 (3 ULg) |
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