References of "KRIDELKA, Frédéric"
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See detailImproved computer-assisted analysis of the global lymphatic network in human cervical tissues.
Balsat, Cédric ULg; Signolle, Nicolas; GOFFIN, Frédéric ULg et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (2013), sous presse

Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters ... [more ▼]

Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters requires an objective characterization of the lymphatic vasculature. Here, we performed a global analysis of the lymphatic network using a new computerized method applied on whole uterine cervical digital images. Sixty-eight cases of cervical neoplasia (12 CIN3, 10 FIGO stage 1A and 46 stage IB1) and 10 cases of normal cervical tissue were reacted with antibodies raised against D2-40, D2-40/p16 and D2-40/Ki67. Immunostained structures were automatically detected on whole slides. The lymphatic vessel density (D2-40), proliferating lymphatic vessel density (D2-40/ki67) and spatial lymphatic distribution in respect to the adjacent epithelium were assessed from normal cervix to early cervical cancer and correlated with lymphovascular space invasion and lymph node status. Prominent lymphatic vessel density and proliferating lymphatic vessel density are detected under the transformation zone of benign cervix and no further increase is noted during cancer progression. Notably, a shift of lymphatic vessel distribution toward the neoplastic edges is detected. In IB1 cervical cancer, although intra- and peritumoral lymphatic vessel density are neither correlated with lymphovascular space invasion nor with lymph node metastasis, a specific spatial distribution with more lymphatic vessels in the vicinity of tumor edges is predictive of lymphatic dissemination. Herein, we provide a new computerized method suitable for an innovative detailed analysis of the lymphatic network. We show that the transformation zone of the benign cervix acts as a baseline lymphangiogenic niche before the initiation of neoplastic process. During cancer progression, this specific microenvironment is maintained with lymphatic vessels even in closer vicinity to tumor cells.Modern Pathology advance online publication, 6 December 2013; doi:10.1038/modpathol.2013.195. [less ▲]

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See detailAssuétudes et grossesse: comment détruire un projet de naissance
Emonts, Patrick ULg; MASSON, Véronique ULg; CHANTRAINE, Frédéric ULg et al

in Revue Médicale de Liège (2013), 68(5-6), 239-244

Les femmes enceintes sont conscientes que toute forme d’addiction durant leur grossesse peut être préjudiciable à leur enfant. Pourtant, de nombreuses gestantes continuent de fumer, de boire de l’alcool ... [more ▼]

Les femmes enceintes sont conscientes que toute forme d’addiction durant leur grossesse peut être préjudiciable à leur enfant. Pourtant, de nombreuses gestantes continuent de fumer, de boire de l’alcool, de consommer des drogues illicites ou d’absorber des médicaments, car ces dépendances sont particulièrement tenaces. Le quatuor de tête en termes de préjudice fœtal est composé du tabac, de l’alcool, de la cocaïne et de l’ecstasy. La période de grossesse est le meilleur moment pour mettre fin à ces addictions. Aussi, est-il indispensable de sensibiliser le grand public, les pouvoirs politiques ainsi que les médecins sur le fait que les assuétudes durant la grossesse représentent une inégalité de santé et d’espérance de vie importante pour l’enfant à naître. [less ▲]

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See detailMatrix metalloproteinase-2 governs lymphatic vessel formation as an interstitial collagenase.
Detry, Benoît ULg; Erpicum, Charlotte ULg; Paupert, Jenny ULg et al

in Blood (2012), 119(21), 5048-56

Lymphatic dysfunctions are associated with several human diseases, including lymphedema and metastatic spread of cancer. Although it is well recognized that lymphatic capillaries attach directly to ... [more ▼]

Lymphatic dysfunctions are associated with several human diseases, including lymphedema and metastatic spread of cancer. Although it is well recognized that lymphatic capillaries attach directly to interstitial matrix mainly composed of fibrillar type I collagen, the interactions occurring between lymphatics and their surrounding matrix have been overlooked. In this study, we demonstrate how matrix metalloproteinase (MMP)–2 drives lymphatic morphogenesis through Mmp2-gene ablation in mice, mmp2 knockdown in zebrafish and in 3D-culture systems, and through MMP2 inhibition. In all models used in vivo (3 murine models and thoracic duct development in zebrafish) and in vitro (lymphatic ring and spheroid assays), MMP2 blockage or down-regulation leads to reduced lymphangiogenesis or altered vessel branching. Our data show that lymphatic endothelial cell (LEC) migration through collagen fibers is affected by physical matrix constraints (matrix composition, density and cross-linking). Transmission electron microscopy (TEM) and confocal reflection microscopy using DQ-collagen highlight the contribution of MMP2 to mesenchymal-like migration of LEC associated with collagen fiber remodeling. Our findings provide new mechanistic insight into how LEC negotiate an interstitial type I collagen barrier and reveal an unexpected MMP2-driven collagenolytic pathway for lymphatic vessel formation and morphogenesis. [less ▲]

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See detailAberrant promoter methylation and expression of UTF1 during cervical carcinogenesis.
Guenin, Samuel; Mouallif, Mustapha ULg; Deplus, Rachel et al

in PLoS ONE (2012), 7(8), 42704

Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern ... [more ▼]

Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern of stem cell (SC) markers during tumor development. In this study, we were interested to identify SC genes methylation profiles during cervical carcinogenesis. A genome-wide promoter methylation screening revealed a strong hypermethylation of Undifferentiated cell Transcription Factor 1 (UTF1) promoter in cervical cancer in comparison with normal ectocervix. By direct bisulfite pyrosequencing of DNA isolated from liquid-based cytological samples, we showed that UTF1 promoter methylation increases with lesion severity, the highest level of methylation being found in carcinoma. This hypermethylation was associated with increased UTF1 mRNA and protein expression. By using quantitative RT-PCR and Western Blot, we showed that both UTF1 mRNA and protein are present in epithelial cancer cell lines, even in the absence of its two main described regulators Oct4A and Sox2. Moreover, by immunofluorescence, we confirmed the nuclear localisation of UTF1 in cell lines. Surprisingly, direct bisulfite pyrosequencing revealed that the inhibition of DNA methyltransferase by 5-aza-2'-deoxycytidine was associated with decreased UTF1 gene methylation and expression in two cervical cancer cell lines of the four tested. These findings strongly suggest that UTF1 promoter methylation profile might be a useful biomarker for cervical cancer diagnosis and raise the questions of its role during epithelial carcinogenesis and of the mechanisms regulating its expression. [less ▲]

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See detailLes cancers viro-induits: interrelations genetique-environnement
Delvenne, Philippe ULg; Renoux, V. M.; Arafa, M. et al

in Revue Médicale de Liège (2012), 67(5-6), 381-9

Among cancers diagnosed worldwide on a yearly basis, 20% are thought to be associated with a viral infection. The viruses involved are, by order of decreasing incidence, the hepatitis viruses, the ... [more ▼]

Among cancers diagnosed worldwide on a yearly basis, 20% are thought to be associated with a viral infection. The viruses involved are, by order of decreasing incidence, the hepatitis viruses, the papillomaviruses and the Epstein-Barr virus. These virus-induced cancers generate a high level of interest not only for the study of mechanisms involved in the neoplastic transformation, but also for the set-up of specific immunotherapies including prophylactic and therapeutic antitumor vaccination. [less ▲]

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See detailRadiothérapie dans le cadre d’une maladie de Paget touchant la région génitale
WERENNE, Xavier ULg; HERMESSE, Johanne ULg; PIRET, Pascal ULg et al

in Revue Médicale de Liège (2012), 67(2), 61-63

Une maladie de Paget urogénitale est habituellement traitée par chirurgie. Cependant, dans certains cas, que ce soit dans le contexte d’une récidive ou à l’évocation d’une chirurgie mutilante, la ... [more ▼]

Une maladie de Paget urogénitale est habituellement traitée par chirurgie. Cependant, dans certains cas, que ce soit dans le contexte d’une récidive ou à l’évocation d’une chirurgie mutilante, la radiothérapie peut être une alternative. Nous relatons le cas d’une patiente traitée avec succès par radiothérapie. [less ▲]

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See detailFrom the Clinics to the Bench and back to the Clinics: design of a medical treatment for Cervical Intraepithelial Neoplasia (CIN)
Jost, Maud; Frankenne, Francis; Maillard, Catherine ULg et al

Conference (2011, May 20)

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See detailWhole Slide Quantification of Stromal Lymphatic Vessel Distribution and Peritumoral Lymphatic Vessel Density in Early Invasive Cervical Cancer: A Method Description
Balsat, Cédric ULg; Blacher, Silvia ULg; Signolle, Nicolas et al

in ISRN Obstetrics and Gynecology (2011), 2011

Peritumoral Lymphatic Vessel Density (LVD) is considered to be a predictive marker for the presence of lymph node metastases in cervical cancer. However, when LVD quantification relies on conventional ... [more ▼]

Peritumoral Lymphatic Vessel Density (LVD) is considered to be a predictive marker for the presence of lymph node metastases in cervical cancer. However, when LVD quantification relies on conventional optical microscopy and the hot spot technique, interobserver variability is significant and yields inconsistent conclusions. In this work, we describe an original method that applies computed image analysis to whole slide scanned tissue sections following immunohistochemical lymphatic vessel staining. This procedure allows to determine an objective LVD quantification as well as the lymphatic vessel distribution and its heterogeneity within the stroma surrounding the invasive tumor bundles. The proposed technique can be useful to better characterize lymphatic vessel interactions with tumor cells and could potentially impact on prognosis and therapeutic decisions. [less ▲]

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See detailLe cas clinique du mois: Issue défavorable d'une grossesse triple
CAPELLE, Xavier ULg; BAVI DIDO, Jean-Vincent ULg; Schaaps, Jean-Pierre ULg et al

in Revue Médicale de Liège (2011), 66(9), 466-9

Body stalk anomaly is rarely described in triplet gestation after medically assisted procreation. The relationship between congenital anomaly, multiple pregnancy, and medically assisted procreation is ... [more ▼]

Body stalk anomaly is rarely described in triplet gestation after medically assisted procreation. The relationship between congenital anomaly, multiple pregnancy, and medically assisted procreation is briefly discussed. [less ▲]

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See detailPrise en charge des consequences de la carence oestrogenique apres cancer du sein.
BLERET, Valerie ULg; PINTIAUX, Axelle ULg; BELIARD, Aude ULg et al

in Revue Médicale de Liège (2011), 66(5-6), 385-92

The prevention and the treatment of oestrogen deficiency induced by breast cancer treatments are crucial in the management of patients. The impacts of this deficiency must not be neglected: quality of ... [more ▼]

The prevention and the treatment of oestrogen deficiency induced by breast cancer treatments are crucial in the management of patients. The impacts of this deficiency must not be neglected: quality of life impairments inducing eventually premature withdrawal of hormonotherapies, and excess of bone and cardio-vascular morbidities and mortalities, especially in good prognosis young women. Management strategies of short and long term effects of this deficiency are reviewed and discussed here. [less ▲]

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See detailHormonothérapie du cancer du sein
LIFRANGE, Eric ULg; ANDRE, Chantal ULg; BLERET, Valerie ULg et al

in Revue Médicale de Liège (2011), 66(5-6), 367-371

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See detailCurrent concepts in the pathology and epigenetics of endometrial carcinoma.
Arafa, Mohammad; Somja, Joan ULg; Dehan, Pierre ULg et al

in Pathology (2010), 42(7), 613-7

In the Western world, endometrial carcinoma is the most common malignant tumour of the female genital tract and is the fourth most common cancer in women. Two different clinicopathological subtypes are ... [more ▼]

In the Western world, endometrial carcinoma is the most common malignant tumour of the female genital tract and is the fourth most common cancer in women. Two different clinicopathological subtypes are recognised: the oestrogen-related (type I, endometrioid) and the non-oestrogen related (type II, non-endometrioid). This article reviews the epidemiology, risk factors, genetic alterations during endometrial carcinogenesis, features of tumours and precursors and early detection of the disease. Insights into the epigenetic alterations, with emphasis on DNA methylation during endometrial carcinogenesis, and their diagnostic value are also provided. [less ▲]

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See detailA rare case of placental choriangioma associated with neonatal diffuse hemangomatosis.
Capelle, Xavier ULg; Syrios, Petros ULg; Chantraine, Frédéric ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2009), 38(3), 246-249

Placental chorioangioma is a benign vascular tumor. Lesions larger than 4cm may cause fetal and maternal complications. Its association with disseminated neonatal hemangiomatosis is rarely described. We ... [more ▼]

Placental chorioangioma is a benign vascular tumor. Lesions larger than 4cm may cause fetal and maternal complications. Its association with disseminated neonatal hemangiomatosis is rarely described. We report a case of a large chorioangioma associated with an hydrops foetalis and disseminated neonatal hemangiomatosis. The relationship between placental chorioangioma and hemangioma is briefly discussed. [less ▲]

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See detailHigh frequency of RASSF1A and RARb2 gene promoter methylation in morphologically normal endometrium adjacent to endometrioid adenocarinoma
Arafa, M.; Kridelka, Frédéric ULg; Mathias, Valérie ULg et al

in Histopathology (2008), 53(5), 525-532

Aims: To identify a DNA methylation signature of endometrioid carcinoma of the endometrium (EEC) in the early stages of endometrial carcinogenesis. <br />Methods and results: Archival biopsy specimens of ... [more ▼]

Aims: To identify a DNA methylation signature of endometrioid carcinoma of the endometrium (EEC) in the early stages of endometrial carcinogenesis. <br />Methods and results: Archival biopsy specimens of 39 EECs, 14 cases of atypical hyperplasia (AH), 11 histologically normal endometrial tissues adjacent to EECs and 24 normal control endometrial samples were retrieved. The cases were tested by quantitative methylation-specific polymerase chain reaction with primers hybridizing in the promoter regions of five genes frequently methylated in human cancer (RASSF1A, RARb2, P16, MGMT and GSTPi). Twenty-nine of 39 (74%) EECs and 7/14 (50%) AHs were methylated for the RASSF1A gene, whereas 17/39 (44%) EECs and 6/14 (43%) AHs were positive for the methylation of the RARb2 gene. No significant results were obtained for the other genes (P16, MGMT and GSTPi). Interestingly, 4/11 (36%) and 6/11 (55%) histologically normal endometrial tissues adjacent to EEC showed, respectively, RASSF1A and RARb2 gene methylation. Furthermore, these 11 specimens were microsatellite stable and showed similar proliferative, cell cycle and apoptotic mean labelling indices as the normal endometrial control tissues. <br />Conclusions: Promoter region methylation of RASSF1A and RARb2 genes is an early event in endometrial carcinogenesis. [less ▲]

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See detailIntérêts et voies d'abord de la stadification paraaortique laparoscopique dans les cancers avances du col utérin
Kridelka, Frédéric ULg; Goffin, Frédéric ULg

in Revue Médicale de Liège (2007), 62 Spec No 2

The stage of a cervical neoplasm is defined on clinical criteria as reported by the International Federation of Gynecology and Obstetrics. Treatment planning however is based on this clinical stage, but ... [more ▼]

The stage of a cervical neoplasm is defined on clinical criteria as reported by the International Federation of Gynecology and Obstetrics. Treatment planning however is based on this clinical stage, but moreover on histological variables obtained at surgical staging. Regarding advanced stage cervical cancer, the recommanded treatment consists in a radical radiotherapy together with concomitant chemotherapy. The target volume of the radiation treatment is directly dependent on the paraaortic lymph node status, the radiological evaluation of which remains unsatisfactory due to lack of sensitivity. We report our experience with two laparoscopic approaches (transperitoneal and retroperitoneal) allowing a histological paraaortic nodal status to be obtained prior to initiating the definitive treatment. [less ▲]

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See detailPeut-on sortir de l'embrouillamini relatif aux contraceptifs oraux et aux cancers cutanés?
Pierard, Gérald ULg; Pierard, Claudine ULg; Quatresooz, Pascale ULg et al

in Revue Médicale de Liège (2007), 62(5-6), 463-6

Skin contains various hormonal receptors, particularly those for estrogens, progesterone and androgens. Steroid hormones of oral contraceptives affect the skin, in particular the control of the cell cycle ... [more ▼]

Skin contains various hormonal receptors, particularly those for estrogens, progesterone and androgens. Steroid hormones of oral contraceptives affect the skin, in particular the control of the cell cycle, DNA replication, apoptosis and other cellular functions. Some estrogen-responsive pathways have the potential to promote tumor development. The question of whether oral contraceptives increase the risk for the development of skin cancer, particularly melanoma, remains an area of concern. There is some evidence that steroid hormones present in oral contraceptives do not significantly increase the risk of developing skin cancer when estrogen exposure is not excessive. [less ▲]

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