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See detailCryopréservation d’ovocytes et d’embryons par congélation ou vitrification dans le cadre de l’assistance médicale à la procréation
Vanderzwalmen, Pierre ULg; Ectors, Fabien ULg; Papras, Y et al

in Poncelet, Christophe; Sifer, Christophe (Eds.) Physiologie, pathologie et thérapie de la reproduction chez l’humain (2011)

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See detailSupport fermé: une réalité clinique pour vitrifier en conditions aseptiques les ovocytes et embryons
Vanderzwalmen, Pierre ULg; Zech, Nicolas; Prapas, Y. et al

in Gynécologie Obstétrique & Fertilité (2010), 38(9), 541-546

Vitrification with the use of ‘‘Open’’ carrier devices (Cryoloop, cryotop, cryoleaf, Vitriplug) which allowed the contact with liquid nitrogen has become a more popular way to achieve cooling rate ... [more ▼]

Vitrification with the use of ‘‘Open’’ carrier devices (Cryoloop, cryotop, cryoleaf, Vitriplug) which allowed the contact with liquid nitrogen has become a more popular way to achieve cooling rate superior to 20.000 °C/min. Even though the question of contamination with liquid nitrogen during ultra-rapid cooling and storage remain debatable with the use of ‘‘open’’ devices, it is important to revise the carrier system in a way, which minimizes the risk of contamination. According to the EU tissues and cells directive, it is advisable that the cooling and storage should be carried out in embryo carrier devices ensuring complete separation of the embryos from liquid nitrogen in a way, which minimizes the risk of contamination. The consequence of a reduction in the cooling rate resulting from the heat-insulating barrier of aseptic devices has to be counteracted by gradually increasing intracellular concentrations of cryoprotectants without inducing a toxic effect. We developed an aseptic vitrification method of vitrification for MII oocytes and embryos at different stage of development using the ‘‘VitriSafe’’ as ‘‘closed’’ carrier device. [less ▲]

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