References of "Jacquet, Nicolas"
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See detailLes aspects stratégiques du bioraffinage de seconde génération en Région Wallonne : symbiose industrielle ou autonomie complète ?
Jacquet, Nicolas ULg; Istasse, Thibaut ULg; Berchem, Thomas ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites ... [more ▼]

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites industriels de bioraffinage s’orientent vers la production de biocarburants (bioéthanol ou biodiésel). Le territoire wallon, de par son accès limité aux ressources végétales renouvelables, semble cependant s’orienter spécifiquement et compétitivement vers la production à plus faibles tonnages de bioproduits. Conclusions. Afin de mener à bien cette initiative, cette étude propose d’argumenter le meilleur choix stratégique devant être ciblé par la Wallonie: développer de nouveaux sites de production autonomes (énergétiquement suffisants et avec des filières d’approvisionnement indépendantes) ou s’ancrer à des systèmes ou des installations de production existants (et bénéficier des facilités déjà opérationnelles d’accès à l’énergie et aux matières premières). Le design de nouvelles unités de bioraffinage intégrées à des unités de production existantes semble être la meilleure option à définir pour le territoire wallon. [less ▲]

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See detailMonitoring the laccase reaction of vanillin and poplar hydrolysate
Sòti, Valentin; Jacquet, Nicolas ULg; Apers, Sandra et al

in Journal of Chemical Technology & Biotechnology (in press)

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during ... [more ▼]

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION: The obtained results can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions. [less ▲]

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See detailScalable temperature induced stress for the large-scale production of functionalized Bifidobacteria
Nguyen, Huu Thanh ULg; Razafindralambo, Hary; Richel, Aurore ULg et al

in Journal of Industrial Microbiology & Biotechnology (in press)

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of ... [more ▼]

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2 L, 2 L, 20 L, 200 L and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e. increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role since it explain the differences observed between the test performed at lab-scale and in industrial conditions. [less ▲]

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See detailMetabolic profile of mixed culture acidogenic fermentation of lignocellulosic residues and the effect of upstream substrate fractionation by steam explosion
Perimenis, Anastasios; van Aarle, Ingrid; Nicolay, Thomas et al

in Biomass Conversion and Biorefinery (in press)

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a ... [more ▼]

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a broader use and asks for the development of sustainable techniques that can efficiently separate, recover and valorize the constituting components. In a cascading concept, residual streams of such processes can be further exploited in an attempt to valorize the largest possible fraction of the initial material. Three lignocellulosic substrates, namely dried sugar beet pulp, wheat bran and miscanthus straw, were upstream fractionated by steam explosion to extract the hemicellulose fraction. This study evaluated the valorization of the residual solid fraction through mixed acidogenic fermentation for the production of volatile fatty acids (VFA) as platform chemicals. Batch experiments have been conducted for the reference material (non-treated) and the solid fraction remaining after steam explosion, with and without the addition of an external mixed inoculum. Steam explosion residues contained less hemicellulose than the initial materials. The difference in the fermentation profile between steam explosion residues and non-treated substrates is dependent on the substrate. Maximum total VFA (tVFA) concentration was 18.8 gCOD/kgmixed_liquor, and maximum yield of chemical oxygen demand (COD) conversion into tVFAwas 33 % for the case of non-treated inoculated beet pulp. [less ▲]

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See detailProduction de nanocellulose à partir de pâte à papier: Hydrolyse enzymatique et valorisation des coproduits dans une stratégie de bioraffinage forestier intégré
Bombeck, Pierre-Louis ULg; Richel, Aurore ULg; Jacquet, Nicolas ULg et al

Conference (2016, January 19)

Présentation succincte de la thématique de recherche doctorale et de son contexte

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See detailEtude du potentiel des sous-produits vinicoles pour des applications à haute valeur ajoutée
Berchem, Thomas ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2016, January 19)

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are ... [more ▼]

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are limited to poor value added applications like composting, agricultural spreading or bioethanol. This work focuses on high value added valorisation possibilities of grape pomace. It takes part in the current trend of using natural bioactive molecules in pharmaceutical, cosmetic or food industry, through the extraction of polyphenols. Firstly, chemical characterization is performed on grape pomace from two grape varieties (Cabernet sauvignon and Pinot noir) in order to determine variability. Secondly, the same approach is applied to grape seeds and skins. Finally, a method of extraction of polyphenols is developed. Results shown in this work tend to indicate a high valorisation potential of grape pomace thank to the identification of compounds like bioactive molecules and especially polyphenols (0.7% in seeds and 0.4% in skins) as well as high concentrations of other compounds in seeds (lignin (43-53%), grape oil (12%), proteins (12%)) and in skins (lignin (20%), tartaric acid (4%), proteins (10%)) of which the valorisation potential from wine-making byproducts needs to be explored. [less ▲]

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See detailIndustrial Biological Chemistry laboratory activities
Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2015, November 26)

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See detailIndustrial Biological Chemistry laboratory activities (GxABT-CBI)
Jacquet, Nicolas ULg

Conference (2015, November 26)

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See detailLa chimie biologique industrielle à Gembloux Agro-Bio Tech : Un aperçu de nos activités
Berchem, Thomas ULg; Istasse, Thibaut ULg; Schmetz, Quentin ULg et al

Conference given outside the academic context (2015)

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de ... [more ▼]

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de matrice biologique (déchets agricoles, industriels et forestiers,...) pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailEt si nos déchets devenaient la source de nouveaux produits. Notion d'économie circulaire
Richel, Aurore ULg; Jacquet, Nicolas ULg; Berchem, Thomas ULg et al

Conference given outside the academic context (2015)

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits ... [more ▼]

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. Cette présentation se consacre aux nouveaux produits et polymères générés au départ de ces déchets dans une approche d'économie circulaire et de priorité des usages. [less ▲]

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See detailComparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group)
Kamdem, Irenée ULg; Jacquet, Nicolas ULg; Tiappi Deumaga, Mathias Florian ULg et al

in Waste Management & Research : The Journal of the International Solid Wastes & Public Cleansing Association (2015)

The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the ... [more ▼]

The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the results of a biochemical analysis performed on Williams Cavendish Lignocellulosic Biomass (WCLB) after steam cracking (SC) and steam explosion (SE) pretreatments. Solid (S) and liquid (L) fractions (Fs) obtained from SC pretreatment performed at 180°C (SLFSC180) and 210°C (SLFSC210) generated, after diluted acid hydrolysis, the highest proportions of neutral sugar (NS) contents, specifically 52.82±3.51 and 49.78±1.39 %w/w WCLB’s dry matter (DM), respectively. The highest proportions of glucose were found in SFSC210 (53.56±1.33 %w/w DM) and SFSC180 (44.47±0.00 %w/w DM), while the lowest was found in unpretreated WCLB (22.70±0.71 %w/w DM). Total NS content assessed in each LF immediately after SC and SE pretreatments was less than 2 %w/w of the LF’s DM, thus revealing minor acid autohydrolysis consequently leading to minor NS production during the steam pretreatment. WCLB subjected to SC at 210°C (SC210) generated up to 2.7-fold bioaccessible glucan and xylan. SC and SE pretreatments showed potential for the deconstruction of WCLB (delignification, depolymerisation, decrystallization and deacetylation), enhancing its enzymatic hydrolysis. The concentrations of enzymatic inhibitors such as 2-furfuraldehyde and 5-(hydroxymethyl)furfural from LFSC210 were the highest (41 and 21 µg mL-1, respectively). This study shows that steam pretreatments in general and SC210 in particular are required for efficient bioconversion of WCLB. Yet, biotransformation through biochemical processes (e.g., anaerobic digestion) must be performed to assess the efficiency of these pretreatments. [less ▲]

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See detailEt si nos déchets devenaient la source de nouveaux produits. Notion d'économie circulaire
Jacquet, Nicolas ULg; Istasse, Thibaut ULg; Berchem, Thomas ULg et al

Conference given outside the academic context (2015)

Notre objectif et nos recherches se concentrent sur l'utilisation des déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux ... [more ▼]

Notre objectif et nos recherches se concentrent sur l'utilisation des déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailProduction of biofuels and biomolecules in the framework of circular economy: A regional case study
Jacquet, Nicolas ULg; Haubruge, Eric ULg; Richel, Aurore ULg

in Waste Management & Research : The Journal of the International Solid Wastes & Public Cleansing Association (2015), 33(12), 1121-1126

Faced to the economic and energetic context of our society, it is widely recognised that an alternative to fossil fuels and oil-based products will be needed in the nearest future. In this way ... [more ▼]

Faced to the economic and energetic context of our society, it is widely recognised that an alternative to fossil fuels and oil-based products will be needed in the nearest future. In this way, development of urban biorefinery could bring many solutions to this problem. Study of the implementation of urban biorefinery highlights two sustainable configurations that provide solutions to the Walloon context by promoting niche markets, developing circular economy and reducing transport of supply feedstock. First, autonomous urban biorefineries are proposed, which use biological waste for the production of added value molecules and/or finished products and are energetically self-sufficient. Second,integrated urban biorefineries, which benefit from an energy supply from a nearby industrial activity. In the Walloon economic context, these types of urban biorefineries could provide solutions by promoting niche markets, developing a circular economy model, optimise the transport of supply feedstock and contribute to the sustainable development. [less ▲]

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See detailGenotype contribution to the chemical composition of banana rachis and implications for thermo/biochemical conversion
Tiappi Deumaga, Mathias Florian; Happi Emaga, Thomas; Tchokouassom, Raphael et al

in Biomass Conversion and Biorefinery (2015), 5(4), 409-416

Chemical composition of banana rachis from three varieties (Grande naine, Pelipita, and CRBP969) was ana- lyzed, and the genotype contribution to composition variabil- ity was investigated. Wet chemistry ... [more ▼]

Chemical composition of banana rachis from three varieties (Grande naine, Pelipita, and CRBP969) was ana- lyzed, and the genotype contribution to composition variabil- ity was investigated. Wet chemistry and instrumental analysis procedures (X-ray diffraction, 31P NMR spectroscopy, and thermogravimetry) were used. Some significant differences were found among the three genotypes: GN-AAA genotype was found to be significantly the highest in ash fraction (30.16 %) and the lowest in acid insoluble lignin (6.58 %) at 95 % confidence level. It showed also the highest content in potassium (43.5 % in ash). Implication of compositional dif- ferences on valorization efficiency by biochemical or thermo- chemical pathways was investigated. For this purpose, corre- lation coefficients between compositional characteristics and yields in volatile compounds from pyrolysis and glucose yields from enzymatic saccharification were analyzed. Ash content was revealed to be the main drawback parameter for volatile yields from pyrolysis (r = −0.93), while for glucose yields during saccharification were limited mainly by the con- tent in guaiacyl units of the lignin fraction (r = −0.98). How- ever, a strong and positive correlation was established be- tween the volatiles yield and the acid insoluble lignin content (r = 0.98) Thus, according to these observations and based on their compositional significant differences, GN-AAA was the better candidate for bioconversion pathway while PPT-ABB and CRBP969-AAAB samples were shown to be better can- didates for thermochemical conversion pathway. This work gives important preliminary information for considering ba- nana rachis as an interesting feedstock candidate for biorefinery. [less ▲]

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See detailProduction of biofuels and biobased compounds in urban biorefineries: A new paradigm for green chemistry
Richel, Aurore ULg; Haubruge, Eric ULg; Jacquet, Nicolas ULg

in Organic Chemistry Current Research (2015), 4(2), 88

Urban bio-refining is an original concept aiming at using urban wastes (household wastes, municipal wastes, industrial liquid and/or solid residues and side-products, etc.), mainly of vegetable origin ... [more ▼]

Urban bio-refining is an original concept aiming at using urban wastes (household wastes, municipal wastes, industrial liquid and/or solid residues and side-products, etc.), mainly of vegetable origin, for the production of an array of biofuels and bioproducts. This urban bio-refining concept fits particularly with the economic, geographic and politic contexts and constraints of the Walloon Region (south part of Belgium). Indeed, Walloon Region is a very small territory (area of about 6,504 sq mi) with a temperate climate. Supply feedstock, mainly arising from forestry and agriculture, are thus rather restricted, submitted to importation, and subjected to non-standardized quality. Several examples of our regional strategy, still available on an industrial scale, are herein proposed and detailed. [less ▲]

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See detailLes initiatives commerciales de bioraffinage en Région Wallonne: production de biocarburants et voies de valorisation connexes
Jacquet, Nicolas ULg; Desquay, Lucas ULg; Jadot, Bastien et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2015), 19(2), 197-203

Introduction Biorefining is progressively gaining interest in Wallonia as a complement to the conventional petrochemical industry. Biorefineries are categorized according to the nature of the raw ... [more ▼]

Introduction Biorefining is progressively gaining interest in Wallonia as a complement to the conventional petrochemical industry. Biorefineries are categorized according to the nature of the raw materials they treat (food or non-food) and the nature of their productions (energy and biofuels or biobased compounds). Literature Production of first-generation and second-generation biofuels (bioethanol and biodiesel) is described, as well as their parallel valorisation pathways. A description of the Belgian biobased industry is also provided. Conclusion Diversification of supply chains, as well as the need to promote a circular economy, becomes a priority for the development of biorefining in Wallonia. [less ▲]

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See detailApplication of Steam Explosion as Pretreatment on Lignocellulosic Material: A Review
Jacquet, Nicolas ULg; Maniet, Guillaume ULg; Vanderghem, Caroline ULg et al

in Industrial & Engineering Chemistry Research (2015), 54(10), 2593-2598

Steam explosion is a thermo-mechanicochemical pretreatment which allows the breakdown of lignocellulosic structural components by the action of heating, formation of organic acids during the process, and ... [more ▼]

Steam explosion is a thermo-mechanicochemical pretreatment which allows the breakdown of lignocellulosic structural components by the action of heating, formation of organic acids during the process, and shearing forces resulting in the expansion of the moisture. Two distinct stages compose the steam-explosion process: vapocracking and explosive decompression which include modification of the material components: hydrolysis of hemicellulosic components (mono- and oligosaccharides released), modification of the chemical structure of lignin, and modification of the cellulose crystallinity index, etc. These effects allow the opening of lignocellulosic structures and influence the enzymatic hydrolysis yield of the material. [less ▲]

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See detailOak barks as raw materials for the extraction of polyphenols for the chemical and pharmaceutical sectors: a regional case study
Dedrie, Maxime; Jacquet, Nicolas ULg; Bombeck, Pierre-Louis ULg et al

in Industrial Crops and Products (2015), 70

Despite their potential for chemical recycling, residues from forest harvesting and wood processing are mostly used for industrial applications with low added value (energy, paper pulp, panels). Bark of ... [more ▼]

Despite their potential for chemical recycling, residues from forest harvesting and wood processing are mostly used for industrial applications with low added value (energy, paper pulp, panels). Bark of both oak species, Quercus robur L. 1753, Quercus petraea (Matt.) Liebl. 1784 is a by-product from sawmill and pulp mill activities. Bark is mainly used as a fuel for the same wood plants. The aim of this study is to look at the feasibility of enhancing the value of this material through the extraction of bioactive molecules such as polyphenols (i.e. catechin, gallic and ellagic acids). First, the effect of industrial storage of logs and bark on their polyphenol content was explored. Then, referring to the selection of tan oaks in the past, the question of an optimum harvesting age is addressed in order to maximize the polyphenol content of the barks. In the end, molecular diversity of bark is examined through the identification of molecules of interest, using different chromatographic analyses. The results show an effect of the industrial context and an effect of the raw material age on the chemical properties of the bark. First investigations also highlight molecules of interest and the molecular diversity, which needs to be further explored. [less ▲]

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