References of "Huynen, Pascale"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailEvaluation of the Xpert®GBS LB test (Cepheid) performed on antenatal screening LIM enrichment broth for detection of Streptococcus agalactiae or group B streptococcus, compared to the reference culture method.
MEEX, Cécile ULiege; DUPONT, Audrey; SACHELI, Rosalie ULiege et al

Conference (2017, October 19)

PCR, performed on LIM enrichment broth compared wit the reference method, that is subculture on selective differential agar performed from the same incubated Lim broth inoculated with a vagino-rectal swab ... [more ▼]

PCR, performed on LIM enrichment broth compared wit the reference method, that is subculture on selective differential agar performed from the same incubated Lim broth inoculated with a vagino-rectal swab collected at 35-37 weeks’ gestation. Material/methods: During an 8-months period in 2015-2016, series of consecutive vagino/rectal swabs collected for antenatal GBS screening (at the university hospital of Liege, Belgium) were plated first on Granada agar and then inoculated in selective enrichment LIM broth. The incubated broth was further sub-cultured on Granada and Biorad StrepBselect agars. Moreover, a sterile swab immersed in the same incubated broth was further analyzed by a real-time PCR targeting GBS using the Xpert®GBS LB test on the GeneXpert® system (Cepheid). Results: Among the 288 antenatal screenings included in the study, 48 (16.7%) were positive for GBS using the culture reference method and 51 Xpert®GBS LB test were positive (17.7%), includin the 48 samples positive in culture and 3 additional specimens for witch culture remained negative. Considering the enriched culture as the gold standard, the sensitivity and specificity of the Xpert®GBS LB test were 100% and 98.8% respectively. Conclusions: The Xpert®GBS LB test performed on incubated LIM broth is at least as efficient as selective enriched culture for antenatal screening of GBS. The turnaround-time and hands-on-ti are much shorter for the Xpert® GBS LB but it is more expensive than culture method, which may limit its use. [less ▲]

Detailed reference viewed: 33 (3 ULiège)
Full Text
Peer Reviewed
See detailEvolution of rate and genotypes of resistance to macrolide/lincosamide among invasive Group B Streptococcus (GBS): Development of a multiplex PCR tool for simultaneous detection of ErmB, ErmTr, MefA and LsaC resistance genes.
SACHELI, Rosalie ULiege; DESCY, Julie ULiege; MEEX, Cécile ULiege et al

Poster (2017, October)

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance ... [more ▼]

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance, ermB, ermTR, mefA and one gene for clindamycin-resistance lsaC. AdhP gene amplification was used as control for GBS identification. All(219) GBS isolates from invasive infections in newborns and adults received by the Belgian National Reference Center for GBS in 2015, and control strains were tested for erythromycin/clindamycin susceptibility (disk-diffusion/broth- microdilution) and for detection of resistance genes. Results: PCR products demonstrated the expected respective sizes. The method has been validated successfully according to ISO15189 analytical requirements. Of the 219 isolates, 67(30,67%) w resistant to erythromycin and/or clindamycin: 44/67(65,78%) showed a constitutive-MLS phenotype and 10/67(14,9%) the inducible-MLS phenotype. Among the constitutive-MLS strains, 73% harboured ErmB gene, 13% ErmTR, 7% ErmB+mefA and 7% ermB together with LsaC gene. The inducible-MLS strains harboured mostly ErmTr gene (89%) and the others the ErmB gene. Among the 10/67(14,9%) GBS strains with an M-phenotype (isolated resistance to erythromycin), the MefA gene was exclusively detected. Among the 3(4,48%) strains showing an isolated resistance to clindamycin (L-phenotype), the LsaC gene was detected. Conclusion: The developed multiplex PCR is able to detect simultaneously four genes involved in MLS resistance in GBS. In 2015, 30,6% of the invasive GBS strains isolated in Belgium were resist to macrolides and/or lincosamides. The emergence of the L-phenotype in GBS described since 2010, justifies the relevance to also detect LsaC gene together with ErmB, ErmTr and MefA. [less ▲]

Detailed reference viewed: 29 (3 ULiège)
Full Text
Peer Reviewed
See detailUpdate of the characteristics of Group B Streptococci (GBS) colonizing pregnant women in Belgium: capsular-type distribution, pili characterization, antimicrobial susceptibility profile and Multiple Locus Sequence Types.
SACHELI, Rosalie ULiege; MEEX, Cécile ULiege; DESCY, Julie ULiege et al

Poster (2017, October)

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national ... [more ▼]

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national survey among pregnant woman. For each strain, determination of capsular-polysaccharide type agglutination and PCR, of pili-type by PCR and of antimicrobial susceptibility by disk-diffusion, broth-microdilution and detection of resistant genes by PCR. For serotype III strains, determination sequence-type by Multiple-Locus Sequence-Typing (MLST). Results: Serotype III was the most prevalent (28.5%) followed by serotypes V, Ia, II, IV and Ib (20.4%, 19.9%, 17.8%, 7%, 5.4%). Serotypes VI, VII and IX were found each once. All strains remained susceptible to penicillin (MICs: 0.03-0.125 mg/L) and other beta-lactams tested; 28.7% were resistant to erythromycin and 26.7% to clindamycin. With regards to pili, all 387 strains harboured one the PI-2 variants alone or in combination and 70.3% contained PI-1. The 110 serotype III isolates were resolved into 18 STs. The most common were ST-17 (35.5%) followed by ST-19 (30%) and ST- ST-27, ST-23 (<=5%). Conclusion: Among GBS from colonized pregnant women in Belgium: capsular-type and pili distributions, and MLST profile among type III strains were quite similar to reported data from Europ and USA during the last decade. As showed in this study, penicillin remains the first line drug of choice. On the contrary, resistance rates against macrolides/lincosamide, has reached a plateau since a decade, but it is noteworthy to notify the emergence of strains with isolated resistance to clindamycine. [less ▲]

Detailed reference viewed: 27 (4 ULiège)
See detailSéminaire : role et place du technologue dans un laboratoire
HUYNEN, Pascale ULiege

Scientific conference (2016, December 15)

Detailed reference viewed: 22 (0 ULiège)
See detailHow to use serology in Sexually Transmitted Infections
HUYNEN, Pascale ULiege; LIBOIS, Agnes

Conference (2016, December 02)

Detailed reference viewed: 21 (1 ULiège)
Full Text
Peer Reviewed
See detailLaboratory diagnosis of syphilis
HUYNEN, Pascale ULiege

Conference (2016, September 26)

Detailed reference viewed: 10 (0 ULiège)
See detailBorréliose de Lyme : aspects cliniques et prise en charge thérapeutique
HUYNEN, Pascale ULiege

Scientific conference (2016, June 22)

Detailed reference viewed: 22 (2 ULiège)
Full Text
Peer Reviewed
See detailToxoplasma gondii, cytomegalovirus, and Treponema pallidum infections in pregancy: what's new?
HUYNEN, Pascale ULiege; LAZZAROTTO, Tiziana; PEYRON, François

in Wiener Klinisches Magazin (2016), 1(3), 1-8

Detailed reference viewed: 23 (1 ULiège)
See detailL'automatisation des selles dans un laboratoire de Sérologie
HUYNEN, Pascale ULiege

Conference (2016, April 21)

Detailed reference viewed: 9 (0 ULiège)
Peer Reviewed
See detailSyphilis during pregnancy
HUYNEN, Pascale ULiege

Conference (2016, April 12)

Detailed reference viewed: 11 (1 ULiège)
Full Text
Peer Reviewed
See detailA One-year Survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterisation
SACHELI, Rosalie ULiege; ADJETEY BAHUN, Akolé ULiege; DARFOUF, Rajae ULiege et al

in Clinical Microbiology and Infection (2016), 22(3), 2859-28517

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the ... [more ▼]

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the emergence of this species in the country, the Belgian National Reference Center (NRC) for dermatophytes launched an epidemiological survey on the main anthropophilic dermatophytes causing TC in Belgium and including the genomic characterization of M. audouinii isolates. In total, 116 strains of M. audouinii were confirmed and characterized by the DiversiLab® system (bioMérieux). Six genotypic variants were identified, among which one major group included 90 isolates and the reference strain. Another variant group (11 strains) was exclusively confined to a geographical region in South Belgium. Analysis of epidemiological characteristics of the infected population showed that the main age category concerned 5-9 year-old children with a sex ratio (Male/Female) of 1.97. Data concerning the geographic origin of the family revealed a majority of Belgian nationality (44.7%), suggesting that the contamination has originated in Belgium. Other nationalities primarily originated from Africa. At this time, no clear correlation has been established between one particular strain and a specific country of origin. [less ▲]

Detailed reference viewed: 85 (9 ULiège)
See detailGestion des épidémies à norovirus
HUYNEN, Pascale ULiege; DUMASY, Axelle

Scientific conference (2016, January 19)

Detailed reference viewed: 14 (4 ULiège)
Full Text
Peer Reviewed
See detailHuman norovirus infection in Latin America
da Silva Polo, Tatiane; Peiro, Juliana; Claudio Nogueira Mendes, Luiz et al

in Journal of Clinical Virology (2016), 78

Detailed reference viewed: 18 (6 ULiège)
Full Text
See detailHow efficient and automated can be Serology and Stool Testing?
HUYNEN, Pascale ULiege

in Year (2015), 13(9), 2-4

Detailed reference viewed: 10 (1 ULiège)
Full Text
Peer Reviewed
See detailEpidemiological aspects and genotypic characterization of T.violaceum strains collected during a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULiege; Dekkers, Charlotte; GRAIDE, Hélène ULiege et al

in Mycoses (2015, October), 58(Supplement 4), 189

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC Liège) launched a one-year national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii and T. violaceum strains isolated from hair to the NRC with a form to fill in including epidemiological data. The fungal strains were identified by microscopy or ITS sequencing in case of doubtful identification. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological data were analyzed with the help of a biostatistician. Results Amongst the collected isolates, 23 strains were confirmed as T.violaceum (results concerning the 116 M. audouinii strains have already been reported). Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 0-4 year-old children (n=9, 39,1%) with a sex-ratio M/F of 1.875. Data concerning the geographic origin of the family were present in 82,6% of the cases and reveal that patients were mainly of Ethiopian origin (n=8, 57,9% of known cases). One patient was also from Burundi showing that T. violaceum strains probably circulate mainly in East Africa. The genotypic analysis led to the distinction of 2 variants of T. violaceum. The major group was composed of 17 strains which were mainly collected in the North of Belgium and included also the reference strain (18/23, 83,3%). The other group (6 strains) was close to the major group but the analysis of the spectral superposition showed some differences between these two groups, defining two distinct variants of T. violaceum in the Belgian population. This second variant was mainly recovered from South Belgium (5/6, 83,3%). No correlation could be made between the genotypic group and a particular ethnical origin as Ethiopian subjects were found in both groups. Conclusion The DiversiLab® system proved to be an efficient method to investigate the molecular epidemiology of dermatophytes infections as reported previously for M. audouinii. These results show that two distinct isolates co-exist in Belgium providing evidence of genetic heterogeneity and a possible spread of one genotypic variant in a restricted geographic area or the co-existence of two variants circulating in different African communities. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as age or ethnical origin. [less ▲]

Detailed reference viewed: 59 (18 ULiège)
Peer Reviewed
See detailQuality and Innovation, key factors for laboratory evolution
HUYNEN, Pascale ULiege

Conference (2015, June 23)

Detailed reference viewed: 14 (1 ULiège)
Full Text
Peer Reviewed
See detailQuality and Innovation, key factors for laboratory evolution
HUYNEN, Pascale ULiege

in Clinical Chemistry & Laboratory Medicine (2015, June 23)

Today the laboratory has to face many challenges: constant increase of number of tests to be run, private labs that competes in reaching a lower TAT, disease outbreak that arises without the possibility ... [more ▼]

Today the laboratory has to face many challenges: constant increase of number of tests to be run, private labs that competes in reaching a lower TAT, disease outbreak that arises without the possibility of human control, like the recent mumps outbreak, the need to provide fast results in case of emergency or for transplants, the request to keep high level of traceability of all results, the accreditation of the lab are just some examples. With the same number of operators, year after year, new clinical needs have to be satisfied in a timely manner, with efficiency and without compromise in quality. The solution for us has been, across several year, to look for innovation. Moving from Elisa to chemiluminescence and therefore from open systems to close and state of the art systems, it has allowed us to face with success all those challenges. The availability of more and more infectious disease markers on fully automated analyzers, with good level of performance, have let us to cope with all the changes that have happened across more than a decade. Indeed innovation and quality are fundamental to support properly the laboratory evolution that occurred since today and it is still occurring. Innovation in our laboratory it is also represented by the introduction of automatized tests not only on serum and plasma specimens, but also on CSF (for Lyme disease diagnosis) and on stool matrix. In 2013 in fact we have introduced, among the assays already tested in our laboratory, two assays performed on this matrix, the C. difficile Toxin A&B and GDH, due to the possibility offered by the LIAISON® systems to run all of them on the same serology platform, without crosscontamination. New markers will be available in the near future, and our laboratory will be always able to meet the next clinical needs. [less ▲]

Detailed reference viewed: 79 (4 ULiège)
See detailNorovirus: ce que les praticiens de soins devraient savoir.
HUYNEN, Pascale ULiege

Conference (2015, June 01)

Detailed reference viewed: 31 (2 ULiège)
Full Text
Peer Reviewed
See detailHow efficiency and automated can be serology and stool testing?
HUYNEN, Pascale ULiege

in Clinical Chemistry & Laboratory Medicine (2015), 53(S1), 161

Detailed reference viewed: 20 (0 ULiège)