  Epizootic rabbit enteropathy: comparison of PCR-based RAPD fingerprints from virulent and non-virulent samplesHuybens, Nathalie  ; Houeix, Julien ; et alin Veterinary Journal (2011), 190(3), 416-417 Detailed reference viewed: 26 (6 ULg)Epizootic rabbit enteropathy inoculum (TEC4) antibiograms and antibiotic fractionationHuybens, Nathalie ; Houeix, Julien ; et alin Veterinary Research Communications (2011), 35 Detailed reference viewed: 23 (6 ULg)Biomolecular comparison of bacteriological caecal flora with and without Epizootic Rabbit Enteropathy (ERE) contaminationHuybens, Nathalie ; Houeix, Julien ; et alPoster (2009, April 01) Detailed reference viewed: 42 (18 ULg) Molecular biology techniques to analyze complex Bactrian populationsHuybens, Nathalie ; Mainil, Jacques ; Marlier, Didier in Annales de Médecine Vétérinaire (2009), 153(2), 112-128 Detailed reference viewed: 32 (8 ULg)INOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHYHuybens, Nathalie ; Houeix, Julien ; et alin World Rabbit Science (2009), 17(4), 185-193 The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼] The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲] Detailed reference viewed: 13 (3 ULg)IS EPIZOOTIC RABBIT ENTEROPATHY (ERE) A BACTERIAL DISEASE?Huybens, Nathalie ; Houeix, Julien ; Szalo, Ioan Mihai et alin Proceeding of the 9th World Rabbit Congress (2008, June 12) The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the ... [more ▼] The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step to find the potential bacterial agent(s). In this study, TEC4 was fractionated with two techniques: centrifugation on discontinuous sucrose gradient then cell adhesion. Two selected fractions were inoculated to SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with both fractions. The 16S rDNA gene was amplified in all fractions and in three negative controls and subsequently analyzed with Restriction Fragment Length Polymorphism (RFLP) and Denaturating Gradient Gel Electrophoresis (DGGE). A difference in bacterial DNA composition was found between virulent and non-virulent fractions which reinforce the potential role of bacteria in the etiology of ERE. [less ▲] Detailed reference viewed: 154 (41 ULg)Analyses bactériologiques traditionnelles et moléculaires de l'inoculum de référence de l'entéropathie épizootique du lapin (TEC4) et de ses fractionsHuybens, Nathalie Master of advanced studies dissertation (2007) Epizootic rabbit enteropathy is a digestive disease that induces huge economic losses in European rabbit production units. Its aetiology is still unknown but a reference inoculum (TEC4) can reproduce the ... [more ▼] Epizootic rabbit enteropathy is a digestive disease that induces huge economic losses in European rabbit production units. Its aetiology is still unknown but a reference inoculum (TEC4) can reproduce the pathology. The aim of the study is to find a bacteria or a bacterial DNA sequence specific to this inoculum. The bacterial species found in TEC4 were separated by centrifugation on discontinuous sucrose gradient followed or not by a cellular adhesion sorting. The rabbit kidney cells adhering fractions can reproduce the pathology by inoculation on sensitive rabbits. The classical bacteriological and bacterioscopical analyses show a variation and a selection of the flora in the course of the fractionation. Restriction Fragment Length Polymorphism (RFLP) analysis with Acc I show a specific pattern for the fraction able to reproduce the pathology. [less ▲] Detailed reference viewed: 66 (6 ULg)Nouveaux animaux de compagnie : infections à Salmonella enterica et état sanitaireHuybens, Nathalie ; ; Poulipoulis, Anastassios et alin Annales de Médecine Vétérinaire (2006), 150(4), 241-246 Human transmission of Salmonella enterica from new companion animals is usually suspected even though very few data according Salmonella infections in these animal species are available. The prevalence of ... [more ▼] Human transmission of Salmonella enterica from new companion animals is usually suspected even though very few data according Salmonella infections in these animal species are available. The prevalence of Salmonella enterica infections in the digestive tract of 450 new companion animals ( 45 ferrets, 7 birds, 308 rodents, 82 rabbits and 8 reptiles) coming from pet shops or individual owners was determined by bacteriological examinations of anal swabs or pooled fresh faeces. Only one Salmonella Enteritidis strain was isolated from a ferret in a pet shop. The symptomatic or asymptomatic Salmonella carriage in ferrets, rodents and rabbits is thus not as frequent as what was suspected. [less ▲] Detailed reference viewed: 73 (13 ULg) |
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