  Chronic fluoxetine treatment and maternal adversity differentially alter neurobiohavioral outcomes in rat damPawluski, Jodi  ; Charlier, Thierry ; Fillet, Marianne et alin Behavioural Brain Research (in press) Detailed reference viewed: 36 (12 ULg)A validated microfluidics-based LC-chip-MS/MS method for the quantitation of fluoxetine and norfluoxetine in rat serumHoubart, Virginie ; Servais, Anne-Catherine ; Charlier, Thierry et alin Electrophoresis (2012) Detailed reference viewed: 9 (3 ULg)Development of a nano-liquid chromatography on chip tandem mass spectrometry method for high-sensitivity hepcidin quantitationHoubart, Virginie ; COBRAIVILLE, Gaël ; Lecomte, Frédéric et alin Journal of Chromatography. A (2011), 1218(50), 9046-9054 Detailed reference viewed: 49 (15 ULg)Optimization of micro-HPLC peak focusing for the detection and quantification of low hepcidin concentrations.Mansion, François ; Chiap, Patrice ; Houbart, Virginie et alin Journal of Separation Science (2011), 34(15), 1820-1827 Micro-high-performance liquid chromatography is a miniaturized, economic and ecological chromatographic system allowing the use of reduced size chromatographic columns. Coupled with electrospray ... [more ▼] Micro-high-performance liquid chromatography is a miniaturized, economic and ecological chromatographic system allowing the use of reduced size chromatographic columns. Coupled with electrospray ionization tandem mass spectrometry, this technique can be used to detect and quantify low concentrations of peptides. In this study, hepcidin was used as the model compound and analysed using octadecylsilica stationary phase by means of a gradient elution mode at a flow rate of 4 muL/min. Several parameters were studied to optimize peak focusing. Using the methodology of experimental design, the mobile-phase gradient conditions and the sample composition were optimized in order to maximize the sensitivity and minimize retention time. Stability of the target peptide in solution was also demonstrated. [less ▲] Detailed reference viewed: 36 (16 ULg)Development and Prevalidation of a Nano-LC-Chip-MS/MS Method for High Sensitivity Hepcidin QuantitationHoubart, Virginie ; Lecomte, Frédéric ; COBRAIVILLE, Gaël et alPoster (2011, June 20) Detailed reference viewed: 33 (12 ULg)Pre-study and in-study validation of a SPE-LC-MS-MS method for the determination of 5-S-cysteinyldopa, a melanoma biomarker, in human plasma.; Mansion, François ; Houbart, Virginie et alin Talanta (2011), 84(2), 280-6 The incidence of malignant melanoma has increased over the past decades, particularly in Caucasian population. This disease presents defavourable prognosis in terms of survey, especially when detection ... [more ▼] The incidence of malignant melanoma has increased over the past decades, particularly in Caucasian population. This disease presents defavourable prognosis in terms of survey, especially when detection occurs at the metastatic phase. Reliable analytical methods for biomarker determination are thus an interesting tool in pathology detection and follow-up. In this context, a method using SPE-LC-ESI-MS-MS for the determination of 5-S-cysteinyldopa (5-SCD) in human plasma was optimized. The presence of matrix effect was investigated in details while 5-SCD stability was studied according to FDA requirements for the validation of bioanalytical methods. Pre-study and in-study validations of the entire method were then successfully performed by applying the approach based on total measurement error and accuracy profiles over a concentration ranges from 1.6 to 200 ng/ml. Good results with respect to accuracy, trueness and precision were obtained. The maximum risk of observing future measurements falling outside the acceptance limits during routine analysis was also estimated. [less ▲] Detailed reference viewed: 15 (6 ULg)A LC-chip nanoelectrospray MS/MS method for hepcidin quantitation in human plasmaHoubart, Virginie ; Lecomte, Frédéric ; Cobraiville, Gaël et alPoster (2010, September) Detailed reference viewed: 80 (23 ULg) Développement et validation d'une méthode de dosage de la glucosamine par SPE/LC/MS dans le plamsa caninHubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alScientific conference (2010, January 26) Detailed reference viewed: 98 (25 ULg)Development and validation of a sensitive solid phase extraction/hydrophilic interaction liquid chromatography/mass spectrometry method for the accurate determination of glucosamine in dog plasma.Hubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alin Journal of Chromatography. A (2010), 1217 A sensitive and accurate LC/MS method was developed for the monitoring of glucosamine (GLcN) dog plasmatic concentration. In this scope, relatively low plasmatic concentrations of GLcN were expected ... [more ▼] A sensitive and accurate LC/MS method was developed for the monitoring of glucosamine (GLcN) dog plasmatic concentration. In this scope, relatively low plasmatic concentrations of GLcN were expected, ranging from 50 to 1000ng/mL. Liquid chromatography coupled to simple quadrupole mass spectrometry detection (LC/MS) was selected bringing the selectivity and the sensitivity needed for this application. Additionally, a solid phase extraction (SPE) step was performed to reduce matrix and ion suppression effects. Due to the ionisable character of the compound of interest, a mixed-mode strong cation exchange (Plexa PCX) disposable extraction cartridge (DEC) was selected. The separation was carried out on a Zorbax SB-CN column (5mum, 4.6mm i.d.x250mm), considering hydrophilic interaction liquid chromatography (HILIC). Indeed, the mobile phase was made of methanol and 5mM ammonium hydrogen carbonate buffer at pH 7.5 (95/5, v/v). The detection was led at m/z ratios of 180.0 and 417.0, for GLcN and IS, respectively. Reliability of the results was demonstrated through the validation of the method using an approach based on the accuracy profile allowing managing the risk associated to the use of these methods in routine analysis: it is thus guaranteed that each future result will fall in the +/-30% acceptance limits with a probability of at least 90%. Successful application of the method to a preliminary pharmacokinetic study illustrated the usefulness of the method for pre-clinical studies. [less ▲] Detailed reference viewed: 149 (56 ULg)Développement et validation d'une méthode de dosage de la glucosamine par SPE/HILIC/MS dans le plamsa de chienHubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alPoster (2009, December 01) Detailed reference viewed: 76 (20 ULg) Development and validation of a sensitive SPE/LC/MS method for the determination of glucosamine in dog plasmaHubert, Cédric ; Houari, Sabah ; Houbart, Virginie et alPoster (2009, June) Detailed reference viewed: 52 (16 ULg)EVALUATION DE DEUX POLYMERES A EMPREINTES MOLECULAIRES POUR LE DOSAGE DU p-[18F]MPPF DANS LE PLASMALecomte, Frédéric ; Aerts, Joël ; Houbart, Virginie et alPoster (2007) Detailed reference viewed: 50 (15 ULg) Supports visuels réalisés dans le cadre des travaux pratiques de Chimie Analytique (Partie I)Ziemons, Eric ; Houbart, Virginie ; Lejeune, Robert et alLearning material (2007) Plusieurs vidéos (utilisation de la burette, calibration de la balance analytique, nettoyage de la balance analytique, gravimétrie, rinçage des pipettes, prélèvement à l'aide d'une pipette à 1 trait ... [more ▼] Plusieurs vidéos (utilisation de la burette, calibration de la balance analytique, nettoyage de la balance analytique, gravimétrie, rinçage des pipettes, prélèvement à l'aide d'une pipette à 1 trait, prélèvement à l'aide d'une pipette à 2 traits et transfert quantitatif) illustrent le B.A.-BA des manipulations des travaux pratiques de 2ème Baccalauréat en Sciences pharmaceutiques. [less ▲] Detailed reference viewed: 56 (11 ULg) |
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