References of "Heuskin, Stéphanie"
     in
Bookmark and Share    
See detailL'étude des sémiochimiques. Applications en écologie chimique
Heuskin, Stéphanie ULg

Scientific conference (2014, December)

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailPlutella xylostella (L.) infestations at varying temperatures induce the emission of specific volatile blends by Arabidopsis thaliana (L.) Heynh
Hien, Truong Thi Dieu ULg; Delory, Benjamin ULg; Brostaux, Yves ULg et al

in Plant Signaling & Behavior (2014)

The effect of combined abiotic and biotic factors on plant volatile organic compound (VOC) emissions is poorly understood. This study evaluated the VOC emissions produced by Arabidopsis thaliana (L.) Col ... [more ▼]

The effect of combined abiotic and biotic factors on plant volatile organic compound (VOC) emissions is poorly understood. This study evaluated the VOC emissions produced by Arabidopsis thaliana (L.) Col-0 subjected to three temperature regimes (17, 22, and 27 °C) in the presence and absence of Plutella xylostella larvae over two time intervals (0–4 and 4–8 h), in comparison to control plants. The analyses of VOCs emitted by Arabidopsis plants were made by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). It was found that certain volatile groups (e.g., alcohols, ketones, aldehydes, and terpenes) are induced by both single factors (temperature or larval infestation) and combined factors (temperature and larvae interactions), whereas other volatile groups (e.g., isothiocyanates [ITCs] and nitrile) were specific to the experimental conditions. ITCs (mainly 4-methylpentyl isothiocyanate) were emitted from plants subjected to larval infestation at 17 and 27 °C after the two time intervals. The proportions of sulfides (mainly dimethyl disulfide) and 4-(methylthio) butanenitrile were significantly higher on herbivore-infested plants at 22 °C compared to the other treatments. Overall, our findings indicate that changes in all experimental conditions caused significant changes to the VOC emissions of Arabidopsis plants. Therefore, the interaction between temperature and larval feeding may represent an important factor determining the variability of volatile emissions by plants subjected to multiple simultaneous factors. [less ▲]

Detailed reference viewed: 19 (13 ULg)
Full Text
Peer Reviewed
See detailThe composition of cuticular compounds indicates body parts, sex and age in the model butterfly Bicyclus anynana (Lepidoptera)
Heuskin, Stéphanie ULg; Vanderplanck, Maryse; Bacquet, Paul et al

in Frontiers in Ecology and Evolution (2014), 2:37

Chemical communication in insects’ sexual interactions is well-known to involve olfaction of volatile compounds called sex pheromones. In theory, sexual chemical communication may also involve chemicals ... [more ▼]

Chemical communication in insects’ sexual interactions is well-known to involve olfaction of volatile compounds called sex pheromones. In theory, sexual chemical communication may also involve chemicals with low or no volatility exchanged during precopulatory gustatory contacts. Yet, knowledge on this latter type of chemicals is so far mostly restricted to the Drosophila fly model. Here we provide the most comprehensive characterization to date of the cuticular chemical profile, including both volatile and non-volatile compounds, of a model butterfly, Bicyclus anynana. First, we characterized the body distribution of 103 cuticular lipids, mostly alkanes and methyl-branched alkanes, by gas chromatography coupled to mass spectrometry (GC-MS). Second, we developed a multivariate statistical approach to cope with such complex chemical profiles and showed that variation in the presence or abundance of a subset of the cuticular lipids indicated body parts, and traits involved in B. anynana mate choice, namely sex and age. Third, we identified the chemical structure of the 20 most indicative compounds, which were on average more abundant (1346.4 ± 1994.6 ng; mean ± SD) than other, likely less indicative, compounds (225.9 ± 507.2 ng; mean ± SD). Fourth, we showed that wings and legs displayed most of the chemical information found on the entire body of the butterflies. Fifth, we showed that non-random gustatory contacts occurred between specific male and female body parts during courtship. The body parts mostly touched by the conspecific displayed the largest between-sex differentiation in cuticular composition. Altogether, the large diversity of cuticular lipids in B. anynana, which exceeds the one of Drosophila flies, and its non-random distribution and evaluation across individuals, together suggest that gustatory information is likely exchanged during sexual interactions in Lepidoptera. [less ▲]

Detailed reference viewed: 41 (4 ULg)
Full Text
Peer Reviewed
See detailTemperature regimes and aphid density interactions differentially influence VOC emissions in Arabidopsis
Hien, Truong Thi Dieu ULg; Delory, Benjamin ULg; Vanderplanck, Maryse et al

in Arthropod-Plant Interactions (2014), 8(4), 317-327

The effects of volatile emissions from plants exposed to individual abiotic and biotic stresses are well documented. However, the influence of multiple stresses on plant photosynthesis and defense ... [more ▼]

The effects of volatile emissions from plants exposed to individual abiotic and biotic stresses are well documented. However, the influence of multiple stresses on plant photosynthesis and defense responses, resulting in a variety of volatile profiles has received little attention. In this study, we investigated how temperature regimes in the presence and absence of the sucking insect Myzus persicae affected volatile organic compound emissions in Arabidopsis over three time periods (0-24 h, 24-48 h, and 48-72 h). Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry was used to evaluate Arabidopsis volatile organic compounds. The results showed that under laboratory conditions, eight volatile classes [alcohols (mainly 2-ethyl-hexan-1-ol), ketone (6-methyl hept-5-en-2-one), esters (mainly (Z)-3-hexenyl acetate), aldehydes (mainly phenylacetaldehyde), isothiocyanates (mainly 4-methylpentyl isothiocyanate), terpenes (mainly (E,E)-α-farnesene), nitrile (5-(methylthio) pentanenitrile), and sulfide (dimethyl trisulfide)] were observed on plants exposed to stress combinations, whereas emissions of six volatile classes were observed during temperature stress treatments alone (with the exception of nitriles and sulfides). Aphid density at high temperature combinations resulted in significantly higher isothiocyanate, ester, nitrile and sulfide proportions. The results of the present study provide an insight into the effects of temperature - aphid interactions on plant volatile emissions. [less ▲]

Detailed reference viewed: 54 (20 ULg)
Full Text
See detailBelgian Grasshoppers: A Nutritious Food Source
Paul, Aman ULg; Frederich, Michel ULg; Blecker, Christophe ULg et al

Poster (2014, May 14)

Rapid urbanization and rising economies are creating shifts in the composition of global food demand, so it is necessary to explore new sources of food with better nutritional profile. Among the ... [more ▼]

Rapid urbanization and rising economies are creating shifts in the composition of global food demand, so it is necessary to explore new sources of food with better nutritional profile. Among the alternative food that exists are the grasshoppers, about 80 species of which are consumed worldwide. Grasshoppers are not only rich source of proteins and lipids but also some important minor component like vitamins and minerals. Edible species of grasshopper in Belgium were identified and attempts were made for the lab rearing of meadow grasshopper (Chorthippus parallelus). The lipids as well as protein contents of meadow grasshopper (Chorthippus parallelus) & long winged conehead (Conocephalus discolor) were investigated. The fatty acid compositions of these two species were determined by gas chromatography. Some of the physicochemical properties of the lipids extracted were also analyzed. These two grasshopper species could be really nutritious source of food. [less ▲]

Detailed reference viewed: 95 (24 ULg)
Peer Reviewed
See detailA pheromone-based formulation against phytophagous pests
Fassotte, Bérénice ULg; Vandereycken, Axel ULg; Heuskin, Stéphanie ULg et al

Poster (2014, April 02)

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or ... [more ▼]

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or indirect biocide activity is one promising alternative option. Our researches focus on the identification of appropriate formulations releasing volatile organic compounds that are attractive for natural enemies of insect pests. However, the elaboration of slow-release devices that ensure stable and controlled release of active volatile compounds is quite challenging. Here, we developed a formulation based on E-β-farnesene and (–)-β-caryophyllene, these two semiochemicals having strong attractive potential on aphid natural enemies including ladybeetles and hoverflies. Both compounds were encapsulated together in alginate gel beads. The blend efficiency is currently being evaluated through laboratory and field assays. [less ▲]

Detailed reference viewed: 48 (8 ULg)
Full Text
See detailChemical analyses of the seeds from Prunella vulgaris: A chemotaxonomic approach
Paul, Aman ULg; Frederich, Michel ULg; Cieckiewicz, Ewa ULg et al

Poster (2014, April)

Common self-heal (Prunella vulgaris) plants are traditionally sown along the border of crops to enhance the biodiversity. Besides enhancing the biodiversity, they can also be a source of interesting ... [more ▼]

Common self-heal (Prunella vulgaris) plants are traditionally sown along the border of crops to enhance the biodiversity. Besides enhancing the biodiversity, they can also be a source of interesting compounds which could be important for food, pharmaceutical and cosmetic industries. The seeds of Common Yarrow were investigated for proteins, fatty acid compositions and polyphenolic compounds. The protein content was analyzed according to Dumas method, the extraction of oil was done using a cold extraction technique employing 2:1 chloroform/methanol as solvent, the fatty acid composition was determined using the gas chromatography and the amount of polyphenolic compounds were estimated using the method as described in European Pharmacopoeia, 8th edition. Common self-heal seeds can be of great commercial importance. [less ▲]

Detailed reference viewed: 42 (6 ULg)
Full Text
See detailSome Interesting Sources of Plant Seed Oil
Paul, Aman ULg; Danthine, Sabine ULg; Blecker, Christophe ULg et al

Poster (2014, March 05)

There is a growing realization worldwide that biodiversity is fundamental to agricultural production and food security, as well as a valuable ingredient of environmental conservation. Flowering strips ... [more ▼]

There is a growing realization worldwide that biodiversity is fundamental to agricultural production and food security, as well as a valuable ingredient of environmental conservation. Flowering strips around the border of the crops serves as an important function to improve the biodiversity, besides this they play a major role in the ruminant nutrition and serve as a source of numerous beneficial compounds. It is well known that seeds store their food reserves for next generation mainly in the form of lipids; some of the seeds from these flowering strips could be an interesting source of lipids. These seed oils could play important role in food, pharmaceutical, cosmetics and other industries. The extraction of seed oil from four such plant species in Belgium namely Oregano (Origanum vulgare), Yellow Bedstraw (Galium verum), Common Self-heal (Prunella vulgaris) & Purple loosestrife (Lythrum salicaria) was carried out. Extraction was done by a cold extraction technique using chloroform/methanol in 2:1 ratio as solvent. Amount of oil extracted from Oregano, Yellow Bedstraw, Common Self-heal and Purple loosestrife was 22.58±0.03 %, 3.28±0.01 %, 14.84±0.12 % & 20.32±0.15 %. The fatty acid profiles of these four species were determined by gas chromatography (using methyl esters of their fatty acids); Oleic acid and Linoleic acid were found in all the four species, Gamma-linolenic acid was found in Purple loosestrife & Alpha-linolenic acid was found in Oregano and Common Self-heal plant species. Thermal behaviour of these four plant seed oils were analyzed using Differential Scanning Calorimetry (DSC), while some other physicochemical properties of the seed oils were also analyzed. These plant seed oils can be of great commercial importance. [less ▲]

Detailed reference viewed: 61 (19 ULg)
Peer Reviewed
See detailAcar'up, a new trapping device for house dust mites
Mailleux, Anne-Catherine; Deneubourg, Jean-Louis; Detrain, Claire et al

Poster (2014)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailHow to get rid of the two spotted spider mites?
Attia, Sabrine; Grissa, Kaouthar Lebdi; Heuskin, Stéphanie ULg et al

in Revue des Régions Arides (2014), 35(3), 1739-1748

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion ... [more ▼]

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion bioassay without allowing direct contact. Responses varied according to oil type and dose, and mite species. The chemical analyses with GC-MS and GC-FID revealed that the two oils differed in their most abundant components. The most abundant components in the Citrus oil were linalyl acetate (41.95%), sabinene (18.60%) and linalool (18.14%) whereas pulegone (41.86%) and menthone (28.33%) were most prevalent in the Mentha oil. Mortality and fecundity were measured with 15 oils concentrations ranged from 0.01 to 8μl/l of air. Tetranychid mortality increased with increasing concentrations with LC50 value of 5.39 and 4.09 μl/l for C. aurantium and M. pulegium, respectively. However few mortality was observed in the case of P. persimilis with LC50 value of 0.46 and 0.26 μl/l for C. aurantium and M. pulegium respectively. For both oils a reduction of fecundity was observed at 0.01 μ/l in the case of T. urticae. The essential oils described herein have potential interest as fumigants for the bio-control of T. urticae. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailIdentification of 1-methyloctyl butanoate as the major sex pheromone component from females of the saddle gall midge, Haplodiplosis marginata (Diptera: Cecidomyiidae)
Censier, Florence ULg; Fischer, Christophe ULg; Laurent, Pascal et al

in Chemoecology (2014)

The saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae), has undergone a resurgence recently as a pest of cereals in Belgium and other European countries. An effective ... [more ▼]

The saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae), has undergone a resurgence recently as a pest of cereals in Belgium and other European countries. An effective monitoring tool of saddle gall midge flights is needed in order to understand the enigmatic population dynamics of this pest, and to design an integrated management strategy. Therefore, volatile compounds emitted by females (alkan-2-ols and alk-2-yl butanoates) were identified, and the chirality of the emitted esters was determined to be the R absolute configuration. In field-trapping experiments, racemic non-2-yl butanoate attracted substantial numbers of H. marginata males. Thus, this compound will be useful in baited traps for monitoring seasonal flight patterns, and improving integrated management of the saddle gall midge in agricultural systems. [less ▲]

Detailed reference viewed: 89 (17 ULg)
Full Text
Peer Reviewed
See detailVOC emissions and protein expression mediated by the interactions between herbivorous insects and Arabidopsis plant. A review
Hien, Truong Thi Dieu ULg; Heuskin, Stéphanie ULg; Delaplace, Pierre ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2014), 18(3), 455-464

Herbivorous insects, such as phloem-sap feeders and chewers, induce resistance response in plants. There is a long-standing hypothesis that herbivores increase the emission of volatile organic compounds ... [more ▼]

Herbivorous insects, such as phloem-sap feeders and chewers, induce resistance response in plants. There is a long-standing hypothesis that herbivores increase the emission of volatile organic compounds (VOCs) in the Arabidopsis plant model. However, most works were restricted to the study of the regulation of plant VOC emissions and only in some cases to the effects of insects on such emissions. Often these investigations do not establish a link between quantitative and qualitative emission of plant VOCs with actual damages caused by insects. Moreover, information remain limited about the processes that occur at the protein level encoded of the host plant under stress conditions. Here, we briefly summarize the effects of specific chewing and phloem-sap feeding insects on the emission of VOCs by Arabidopsis thaliana Col-0, and review some predictions about pathogenesis-related proteins, based on current evolutionary hypotheses. Further investigation of the effects of herbivorous insects on VOC emissions and protein expression is expected to improve our knowledge about their patterns and functions in plant responses to stresses. [less ▲]

Detailed reference viewed: 42 (6 ULg)
Peer Reviewed
See detailThe facultative bacterial symbiont Serratia symbiotica in Acyrtosiphon pisum confer resistance to Aphidius ervi
Attia, Sabrine; Foray, Vincent; Louâpres, Philippe et al

Poster (2014)

Detailed reference viewed: 38 (9 ULg)
Peer Reviewed
See detailDoes the facultative bacteria Serratia symbiotica influence the foraging strategies of aphid parasitoids?
Attia, Sabrine; Louâpre, Philippe; Foray, Vincent et al

Poster (2014)

Detailed reference viewed: 40 (7 ULg)
See detailLa validation de méthodes d'analyse
Heuskin, Stéphanie ULg

Scientific conference (2013, December)

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailL'étude des sémiochimiques. Applications en écologie chimique
Heuskin, Stéphanie ULg

Scientific conference (2013, November 21)

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailFumigant toxicity of Mentha pulegium and Citrus aurantium essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae)
Attia, Sabrine; Grissa, K.L.; Lognay, Georges ULg et al

in Boeckx; Bossier, Peter; Smagghe, Guy (Eds.) et al Proceedings of the 65th international symposium on crop protection (2013, May 21)

Detailed reference viewed: 19 (0 ULg)
Full Text
See detailDéveloppements analytiques appliqués à une thématique d'écologie chimique
Heuskin, Stéphanie ULg

Scientific conference (2013, February)

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailThe scent of inbreeding: a male sex pheromone betrays inbred males
Van Bergen, E.; Brakefield, P.; Heuskin, Stéphanie ULg et al

Poster (2013)

Inbreeding depression results from mating among genetically related individuals and impairs reproductive success. The decrease in male mating success is usually attributed to an impact on multiple fitness ... [more ▼]

Inbreeding depression results from mating among genetically related individuals and impairs reproductive success. The decrease in male mating success is usually attributed to an impact on multiple fitness-related traits that reduce the general condition of inbred males. However, the reduced mating success of inbred males could also be a consequence of strong selection on females to avoid mating with an inbred male. This is especially relevant if females gain direct benefits through appropriate mate choice (parental care, territory defences, nuptial gifts) or if they suffer direct costs should they mate with an inbred male (decreased offspring viability and fertility). Here we find that the production of the male sex pheromone is reduced significantly by inbreeding in the butterfly Bicyclus anynana. Other traits indicative of the general condition, including flight performance, are also negatively affected in male butterflies by inbreeding. Yet we unambiguously show that only the production of male pheromones affects mating success. Thus, this pheromone signal informs females about the inbreeding status of their mating partners. We also identify the specific chemical component, hexadecanal, likely responsible for the decrease in male mating success. Our results advocate giving increased attention to olfactory communication as a major causal factor of mate-choice decisions and sexual selection. [less ▲]

Detailed reference viewed: 8 (0 ULg)