References of "Heuskin, Stéphanie"
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See detailElevated carbon dioxide concentration reduces alarm signaling in aphids
Boullis, Antoine ULiege; Fassotte, Bérénice ULiege; Sarles, Landry ULiege et al

in Journal of Chemical Ecology (2017), 43

Insects often rely on olfaction to communicate with surrounding conspecifics. While the chemical language of insects has been deciphered in recent decades, few studies have assessed how changes in ... [more ▼]

Insects often rely on olfaction to communicate with surrounding conspecifics. While the chemical language of insects has been deciphered in recent decades, few studies have assessed how changes in atmospheric greenhouse gas concentrations will impact pheromonal communication by insects. Here, we hypothesize that changes in the concentration of atmospheric carbon dioxide (CO2) affect the whole dynamics of alarm signaling in aphids, including: (1) the production of the active compound (E)-β-farnesene (Eβf), (2) emission behavior when under attack, (3) perception by the olfactory apparatus, and (4) the escape response. We reared two strains of the pea aphid Acyrthosiphon pisum under ambient and elevated CO2 concentrations for several generations. We found that an increase in CO2 concentration reduced the production (i.e., individual content) and emission of Eβf (released under predation events). While no difference in Eβf neuronal perception was observed, we found that an increase in CO2 strongly reduces the escape behavior expressed by an aphid colony following exposure to natural doses of the alarm pheromone. In conclusion, our results confirm that changes to greenhouse gases do impact chemical communication in insects, and could potentially have a cascade effect on interactions with higher trophic levels. [less ▲]

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See detailChemical Composition and Acaricidal Activity of Thymus algeriensis Essential Oil against Varroa destructor
Kouache, Benmoussa; Brada, Moussa; Saadi, Abdelkader et al

in Natural Product Communications (2017), 12(0), 1-4

The aim of the present study was to determine the chemical composition and evaluate the acaricidal activity of Thymus algeriensis essential oil (TAEO) against Varroa destructor. This ectoparasitic mite is ... [more ▼]

The aim of the present study was to determine the chemical composition and evaluate the acaricidal activity of Thymus algeriensis essential oil (TAEO) against Varroa destructor. This ectoparasitic mite is a pest of the honey bee Apis mellifera. The essential oil from the aerial parts of T. algeriensis, obtained by hydrodistillation, was obtained in a yield of 2.8± 0.2%, w/w. The TAEO was analyzed by GC and GC/MS. Thirty-four compounds were identified, representing 99.3% of the oil. The main constituents were carvacrol (48.4%), γ-terpinene (14.9%), p-cymene (14.7%), and thymol (5.6%). Four lots were constituted at the level of an apiary in order to study the dynamics of the Varroa destructor and its host, Apis mellifera. After diagnosis by the biological method "install of diapers", the lots were treated at different doses of TAEO (0.1, 0.3 and 0.5%). TAEO was sprayed on top of the hives. The results show that TAEO at 0.5% resulted in a decrease in the rate of infestation of Varroa destructor, causing a mortality rate of 32.6% without negative effect on the nesting of the queen. The essential oil of T. algeriensis could be used as a bioacaricidal agent. [less ▲]

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See detailNutritional Composition and Rearing Potential of the Meadow Grasshopper (Chorthippus parallelus Zetterstedt)
Paul, Aman ULiege; Frederich, Michel ULiege; Uyttenbroeck, Roel ULiege et al

in Journal of Asia-Pacific Entomology (2016), 19(4), 1111-1116

Insects, particularly those belonging to the family Acrididae (grasshoppers), are commonly consumed as human food in many parts of the world. Grasshoppers of the species Chorthippus parallelus are ... [more ▼]

Insects, particularly those belonging to the family Acrididae (grasshoppers), are commonly consumed as human food in many parts of the world. Grasshoppers of the species Chorthippus parallelus are abundantly found throughout Europe. However, these insects were not consumed by Europeans till now, but could possibly be used as human food, which is why we investigated their chemical composition. We found that they contain high level of proteins (69%), with an excellent amino acid profile and protein digestibility (97%). Furthermore, specimens of C. parallelus have an interesting fatty acids profile and minerals composition. Preliminary toxicity assessment indicates that these insects do not exhibit toxicity towards neutrophil cells (white blood cells). These data suggest that C. parallelus could be considered for human consumption. Rearing trials done during the study show that commercial rearing could be developed to produce sufficient biomass for sustaining human consumption. [less ▲]

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See detailValorization of Seeds from Some Field Border Flowering Seeds
Paul, Aman ULiege; Danthine, Sabine ULiege; Mutwale Kapepula, Paulin ULiege et al

Poster (2016, September 20)

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It ... [more ▼]

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It could be really interesting to valorize some commonly grown plant in these strips to render food or health promoting compounds. With this objective in mind the seeds of Achillea millefolium, Anthriscus sylvestris and Prunella vulgaris were investigated for lipids, proteins and phenolic content. Further the lipids were analyzed for fatty acid profile using gas chromatography and the phenolic compounds in the methanolic extract of defatted seeds were identified using HPLC-DAD. The antiradical activity of the methanolic extracts obtained from defatted seeds was investigated using DPPH and ABTS assays. The anti-inflammatory potential of these seed extracts was evaluated on the reactive oxygen species (ROS) production by stimulated neutrophils and on the specific activity of myeloperoxidase (MPO), a pro-oxidant enzyme marker of inflammation. Seeds from all three plants were analyzed with interesting levels of lipids, proteins and phenolic content. Linoleic acid, oleic acid and alpha-linolenic acid were the major fatty acids analyzed in A. millefolium, A. sylvestris and P. vulgaris respectively. On the other hand different phenolic acid formed the major phenolic constituents. Seed extracts displayed high ABTS and DPPH radical-scavenging activities in a dose dependent manner. Also a strong dose dependent anti-inflammatory activity of all three extracts was observed against ROS production by neutrophils and MPO activity. Results indicate that these seed show a great potential to render lipids which could be utilized as human food, further the defatted seeds could be directly included in human diet due to interesting levels of proteins and anti-inflammation ability. [less ▲]

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See detailCan tropical basil be integrated in vegetable crop pest management?
Yarou, Boni Barthélémy ULiege; Verheggen, François ULiege; Heuskin, Stéphanie ULiege et al

Poster (2016, May 17)

To address human and environmental health issues related to the use of synthetic pesticides, it is important to explore other ecological pest management approaches. Within this context, a study was ... [more ▼]

To address human and environmental health issues related to the use of synthetic pesticides, it is important to explore other ecological pest management approaches. Within this context, a study was conducted to evaluate the toxic and repellent effect of Ocimum gratissimum L. (Lamiaceae) on Myzus persicae S. (Aphididae). The toxicity of O. gratissimum essential oil (EO) was evaluated on M. persicae comparing 3 concentrations (0.001%; 0.01% and 0.1%) to a control (15% sucrose). Mortality rate and fecundity were assessed for each concentration. The observations were made daily, for 4 days, on 12 replicates per treatment. To test the repellent effect of O. gratissimum plants, 2 treatments were compared: a group of 8 Amaranthus cruentus L. (Amaranthaceae) plants with 1 plant of O. gratissimum in the middle and a group of 9 A. cruentus plants (control). The distribution of aphids was analyzed 12 days after the middle plant infestation (20 aphids per plant) for each treatment (6 replicates per treatment). O. gratissimum EO was found toxic from a concentration of 0.01%, with a mortality rate of 34% versus 12% for the control ; The fecundity felt from 30 larvae after 4 days (control) to 15 larvae for the 3 doses of the EO. The analysis with the generalized linear mixed model with Poisson error distribution followed by Turkey test (5 %) showed that EO is significantly more toxic than the control, both for mortality and fecundity (p <0.001). In association test, the population of M. persicae (15 aphids per plant) was significantly (p<0,001) lower when A. cruentus plants was associated with O. gratissimum plant than with the control (22 aphids per plant). Furthermore, the population increases gradually as one moves away from the infestation point in association test whereas it decreases in the control. With regards to these results, it appears that O. gratissimum has biocide effects on M. persicae. This plant may be used in an integrated pest management strategy in the production of vegetable to reduce the use of synthetic pesticides and avoid chemicals residues. [less ▲]

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See detailDevelopment of a semiochemical-based control method against the walnut husk fly
Sarles, Landry ULiege; Heuskin, Stéphanie ULiege; Verheaghe, Agnès et al

Conference (2016, May 17)

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See detailQuantification of four Isoflavones in Forages with UPLC®-MS/MS, using the Box-Behnken Experimental Design to Optimize Sample Preparation
Daems, Frédéric ULiege; Romnee, Jean-Michel; Rasse, Catherine et al

in Chromatographia (2016), 79(11), 711-725

A performant method for the simultaneous quantification of daidzein, genistein, formononetin and biochanin A in forages using an UPLC®-MS/MS was developed and fully validated. The ultrasound-assisted ... [more ▼]

A performant method for the simultaneous quantification of daidzein, genistein, formononetin and biochanin A in forages using an UPLC®-MS/MS was developed and fully validated. The ultrasound-assisted extraction and enzymatic hydrolysis used in the sample preparation step were optimized using the Box-Behnken experimental design. The optimal extraction conditions used for a representative mix of forage plants were 80°C, 10 min and 55% methanol, and for hydrolysis they were 20°C, 18 h and pH=6. The chromatographic separation was achieved using an Acquity® UPLC® HSS T3 column, with a water/methanol linear gradient containing 0.01% of formic acid at a 0.55 mL min-1 flow rate. The four isoflavones were detected by ESI mass spectrometry in positive ion MRM mode. The method allows high throughput analyses of samples and showed an adequate linear regression model for all isoflavones over a range from 5 to 125 ng mL-1. There was good intra- and inter-day precision (≤ 8.2% and ≤ 7.6%) and accuracy (≤ 11.4% and ≤ 7.1%). The recovery rates were in an acceptable range of 70-120%, except for biochanin A, where the rate was about 50%. Good method repeatability was also observed, and there was no matrix effect or carry-over problem. The sample extracts were stable for at least 6 days of storage at -21°C and 6°C. The method proved to be sensitive, precise and accurate for discriminating a wide variety of forages likely to be grazed by ruminants according to their isoflavone content and to observe the impact storage process on isoflavone content in forages. [less ▲]

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See detailAnalytical methods used to quantify isoflavones in cow's milk: a review
Daems, Frédéric ULiege; Romnee, Jean-Michel; Heuskin, Stéphanie ULiege et al

in Dairy Science and Technology (2016)

This paper provides an update and comprehensive review of the analytical methods used for quantifying isoflavones and their metabolites in cow’s milk. Isoflavones are secondary plant metabolites that are ... [more ▼]

This paper provides an update and comprehensive review of the analytical methods used for quantifying isoflavones and their metabolites in cow’s milk. Isoflavones are secondary plant metabolites that are similar to 17 β-estradiol in chemical structure. They form one of the most common categories of phytoestrogens. Numerous health benefits have been attributed to isoflavones, but many of these compounds are also considered to be endocrine disruptors, with adverse effects on health. These contradictory trends offer an attractive prospect for future research, and therefore, sensitive and reliable analytical methods are required to clarify various issues about isoflavones. For this review, a structured methodology was used to select 26 relevant articles published between 2005 and 2015 from the Scopus and CAB Abstract databases. The review discusses individual steps of the analytical procedures described in these articles, including sample preparation, instrumental analysis and validation. The most commonly used analytical procedure is sample preparation involving liquid-liquid extraction and an enzymatic hydrolysis step followed by liquid chromatography with mass spectrometry analysis. Currently, however, there is no standardized procedure for the sample preparation and analysis of isoflavones in milk. [less ▲]

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See detailInfluence of the secondary endosymbiont Serratia Symbiotica on the resistance to the parasitism in the aphid Acyrthosiphon pisum
Attia, Sabrine; Foray, Vincent; Louâpre, Philippe et al

in Journal of Entomology and Zoology Studies (2016)

Aphids have an obligate association with the primary symbiont Buchnera aphidicola and is known to affect aphid fitness. Aphids commonly harbour other facultative bacterial endosymbionts and may benefit ... [more ▼]

Aphids have an obligate association with the primary symbiont Buchnera aphidicola and is known to affect aphid fitness. Aphids commonly harbour other facultative bacterial endosymbionts and may benefit from their presence through increased resistance to parasitoids [1]. Present results suggest that the ability of hosts to defend against natural enemies depend not only on the presence of symbionts but also on the host genotype. Aphids represent a complex micro-environment for the parasitoid larvae and both the aphid physiology and endosymbiont influence their survival until mummification. [less ▲]

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See detailChemical Characterization of Essential Oils of Mints from Senegal
Diop, Serigne Mbacké; Guèye, Momar Talla; Ndiaye, Ibrahima et al

in Natural Product Communications (2016), 11(0), 1-2

Mints from Senegal were extracted separately from fresh (F) and shade-dried (D) plants by steam distillation. Yields were of 0.28 and 0.21% for M. citrata L., 0.21 and 0.18% for M. x piperita L. and 0.10 ... [more ▼]

Mints from Senegal were extracted separately from fresh (F) and shade-dried (D) plants by steam distillation. Yields were of 0.28 and 0.21% for M. citrata L., 0.21 and 0.18% for M. x piperita L. and 0.10 and 0.19% for M. spicata L. in the fresh and dried plants, respectively. GC/FID and GC/MS analysis revealed that many of the major compounds of essential oils decreased with drying. The prominent components of M. citrata oils were linalool that constituted 45.8% (F) and 42.0% (D) and linalyl acetate 42.7 (F) and 38.5% (D). Mentha x piperita was characterized by menthofuran with 30.7% (F) and 28.1% (D), menthol 15.9(F) and 16.4% (D), menthone 13.0 (F) and 14.2% (D), pulegone 17.6%(F) and 13.8%(D) and 1.8-cineole 3.7%(F) and 3.4% (D). Mentha spicata contained mainly carvone 67.8 and 74.7% and limonene 18.1 and 12.5% in the fresh and dried plants respectively. [less ▲]

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See detailChemical composition of essential oils and floral waters of Mentha longifolia (L.) Huds. from Senegal
Diop, Serigne Mbacké; Guèye, Momar Talla; Ndiaye, Ibrahima et al

in American Journal of Essential Oils and Natural Products (2016), 4(1), 46-49

Essential oils of Mentha longifolia from Senegal were extracted from fresh plants and dried plants in the shade for 7 days. The yields were of 0.39 and 0.32% in the fresh and dried plants respectively ... [more ▼]

Essential oils of Mentha longifolia from Senegal were extracted from fresh plants and dried plants in the shade for 7 days. The yields were of 0.39 and 0.32% in the fresh and dried plants respectively. Analysis of the essential oils, floral waters and the assay of pulegone were carried out by GC/FID and GC/MS. The major compounds identified in the oils were pulegone (52.0 and 42.4%), menthone (14.3 and 21.2%), 1, 8-cineole (13.1 and 11.4%) and isomenthone (9.0 and 13.2%) in the fresh and dried plants, respectively. Analyses revealed that floral waters were also characterized by the same major compounds as essential oils but at different rates. In these floral waters, pulegone constituted 60.2 and 47.0%, 1,8- cineole 7.9 and 19.6%, isomenthone 7.2 and 10.7%, menthone 6.4 and 9.2%, chrysanthenone 6.4 and 3.2% and α-terpineol 3.0 and 2.7% in the fresh and dried plants, respectively. The assay results of pulegone, a hepatotoxic compound, have shown very high levels (444.6 and 393.3mg/g) in both fresh and dried plants. [less ▲]

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See detailInsecticidal activity of Lavandula angustifolia Mill. against the pea aphid Acyrtosiphum pisum
Attia, Sabrine; Lognay, Georges ULiege; Heuskin, Stéphanie ULiege et al

in Journal of Entomology and Zoology Studies (2016), 4(1), 118-122

The essential oil of Lavandula angustifolia was investigated for its biocide activity against Acyrtosiphum pisum by fumigation. The oil was characterised by GC-MS revealing that linalool was the most ... [more ▼]

The essential oil of Lavandula angustifolia was investigated for its biocide activity against Acyrtosiphum pisum by fumigation. The oil was characterised by GC-MS revealing that linalool was the most abundant component (38.57%), followed by linalyl acetate (29.95%), 1,8-cineole (13,66%), camphor (13,13%), -pinene (3,14%) and terpinene-4-ol (1,54%). The Mortality was measured upon treatment with oil concentrations ranging from 5 to 25 µl.l-1 of air. The mortality of aphids increased with oil concentration and LC50 values were determined to be 11.2 µl.l-1 of air. The full mixture was also prepared and toxicity was compared with individual constituents. The results showed that the presence of all constituents were necessary to equal the toxicity of the natural oils L. angustifolia oil can provide valuable pesticide activity with significantly lower LC50 values [less ▲]

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See detailImpact of climatic factors on the release of E-β-caryophyllene from alginate beads
Daems, Frédéric ULiege; Bera, François ULiege; Lorge, Stéphanie ULiege et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2016), 20(2),

Description of the subject. Alginate beads that release semiochemical compounds are interesting biological control devices used to attract the natural enemies of aphids in infested crops. Little ... [more ▼]

Description of the subject. Alginate beads that release semiochemical compounds are interesting biological control devices used to attract the natural enemies of aphids in infested crops. Little information, however, is available about the impact of climatic factors on the release of semiochemicals from this diffusive system. Objectives. The objective of this scoping study was to investigate the impact of temperature, relative humidity and wind speed, on the release of E-β-caryophyllene from alginate beads. Method. The impact of the three climatic factors on the release of E-β-caryophyllene from alginate beads was evaluated using a Box-Behnken experimental design and a laboratory scale volatile collection system. The influence of relative humidity on bead water content and size, with and without this semiochemical, was also investigated using a gravimetric method and an easy-to-use photographic device, respectively. Results. The results showed that an increase in temperature caused a significant increase in the E-β¬-caryophyllene release rate. Neither relative humidity nor airflow, however, had a significant effect on the release of this semiochemical when relative humidity and wind speed ranged from 33% to 75% and from 6.61 x10-4 m.s-1 to 2.05 x10-2 m.s-1, respectively. The isotherm curves obtained were characteristic of food products and biopolymer materials. The isotherm shapes were not modified by the incorporation of the semiochemical into alginate beads, and showed an important increase in water content when relative humidity values exceeded 85%. Fortunately, this water content increase did not affect bead size, which facilitates the calculation of E-β-caryophyllene release. Conclusion. The present study shows for the first time the impact of the three main climatic factors on the release of E-β-caryophyllene from alginate beads. It allows learning more about how these biological control devices operate in order to optimize future field trials. [less ▲]

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See detailDo changes in atmospheric CO2 concentration affect aphid alarm signaling?
Boullis, Antoine ULiege; Appeldoorn, Claire; Oostrom, Marjolein et al

Conference (2015, November 06)

The effects of global atmospheric changes on vegetation and associated insect populations are increasingly studied, but how these gases affect the interactions between herbivore insects and their natural ... [more ▼]

The effects of global atmospheric changes on vegetation and associated insect populations are increasingly studied, but how these gases affect the interactions between herbivore insects and their natural enemies is less clear. As the efficacy of natural enemies is governed largely by behavioral mechanisms, changes in the behavior of insect prey defenses can change the dynamics of insect populations. The impact of increases in atmospheric carbon dioxide (CO2) concentrations on aphid population dynamic is well documented. However, few publications about their chemical ecology are reported. Aphids are using many chemical signals to communicate with each other or with their environment. For example aphids produce an alarm pheromone to signal the presence of a natural enemy in the colony. For our experiments, aphids Acyrthosiphon pisum (Harris) were reared on Vicia faba L. in home-made Plexiglas® chambers, allowing us to control the CO2 concentration, temperature and humidity. Aphids were reared under both ambient (aCO2 ≈ 400 ppm) and elevated (eCO2 ≈ 800 ppm) CO2 concentration for several generations. Here we quantified the emission of (E)-β-Farnesene (EβF - main compound of alarm pheromone) released by predated aphids reared under ambient or elevated CO2 concentration, with two different methods: a real-time analysis, and the total amount analysis. The EβF content of whole aphid bodies was also analyzed, as well as the escape behavior of aphid colony according to the growing conditions of aphid populations. These results will be discussed in terms of biological control in future climate. [less ▲]

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See detailMultitrophic interactions between plants-viruses-aphidiphagous
Bosquée, Emilie ULiege; Chevalier Mendes Lopes, Thomas ULiege; Boullis, Antoine ULiege et al

Conference (2015, November 06)

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See detailA PHEROMONE TRAP MONITORING SYSTEM FOR THE SADDLE GALL MIDGE, Haplodiplosis marginata (von Roser) (DIPTERA: CECIDOMYIIDAE)
Censier, Florence ULiege; Heuskin, Stéphanie ULiege; SAN MARTIN Y GOMEZ, Gilles et al

in Crop Protection (2015), 80

Outbreaks of saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae) have been reported in Belgium and other European countries since 2010. Because of the sporadic nature of this ... [more ▼]

Outbreaks of saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae) have been reported in Belgium and other European countries since 2010. Because of the sporadic nature of this pest, which can sometimes be very harmful to cereal crops, an effective monitoring tool is required, both to determine the optimal timing for insecticide applications, and to understand the enigmatic population dynamics of this insect. Following the recent identification of the major sex pheromone component of the saddle gall midge, non-2-yl butanoate, a slow-release dispenser was developed using rubber septa. The release rates of 5 mg and 10 mg-loaded dispensers were initially measured under laboratory conditions, and their effectiveness in terms of pheromone loading and use duration was assessed in the field. The experiments showed that sticky traps baited with 5 mg pheromone-loaded rubber dispensers, renewed every 6 weeks, are suitable for accurately monitoring male H. marginata flights. [less ▲]

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See detailComment évaluer un arôme: approche analytique
Heuskin, Stéphanie ULiege

Scientific conference (2015, October)

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