Development and evaluation in vitro and in vivo of injectable hydrolipidic gels with sustained-release properties for the management of articular pathologies such as osteoarthritis.
; Oprenyeszk, Frédéric ; Franck, Thierry et al
in International Journal of Pharmaceutics (2015), 490
This study aimed to evaluate glycerol monooleate (GMO) as a carrier to develop viscoelastic and injectable sustained-release drug delivery systems. The potential pro- and antioxidant activity of the ... [more ▼]
This study aimed to evaluate glycerol monooleate (GMO) as a carrier to develop viscoelastic and injectable sustained-release drug delivery systems. The potential pro- and antioxidant activity of the developed hydrolipidic gels were evaluated by measuring the production of ROS by polymorphonuclear leukocytes (PMNs). In addition, the biocompatibility and effectiveness of two selected gel candidates were evaluated in vivo by evaluating the benefit of a single intraarticular injection of these new treatments in a model of osteoarthritis in rabbits. The in vitro study demonstrated that the carrier F1 did not have a pro-oxidative effect and even protected PMNs against natural auto-activation, regardless of the incorporation of either clonidine chlorhydrate or betamethasone dipropionate. The in vivo study demonstrated that F1 and F1-BDP induced a loss of cartilage quality in comparison to the control and reference groups but that the lesions of cartilage observed were generally mild, with not much full-depth erosion. Moreover, no exacerbating inflammation was observed when considering the synovial membranes and the PGE2 and CRP levels. These results seemed to demonstrate that the sustained-release formulation based on GMO could be well-tolerated after intraarticular injection. Moreover, it could have the potential to prevent inflammatory conditions while sustaining drug activity locally over weeks. [less ▲]Detailed reference viewed: 6 (1 ULg)
Baseline Fibulin3 concentrations are associated with incidence of clinical knee OA after 30 months in overweight and obese women
; Sanchez, Christelle ; Henrotin, Yves et al
in Osteoarthritis and Cartilage (2015, April), 23(S2), 83Detailed reference viewed: 10 (0 ULg)
COMBINED CHONDROITIN SULFATE AND GLUCOSAMINE IS MORE EFFICIENT THAN CELEBREX IN REDUCING SERUM LEVELS OF COLL2-1, A CARTILAGE DEGRADATION BIOMARKER, IN PATIENTS WITH SEVERE OA: RESULTS FROM A RANDOMIZED, DOUBLEBLIND, MULTICENTRIC CLINICAL TRIAL
Henrotin, Yves ; ; et al
in Osteoarthritis and Cartilage (2015), 23(S2), 86Detailed reference viewed: 14 (3 ULg)
ANALGESIC EFFICACY AND SAFETY OF CURCUMINOIDS IN CLINICAL PRACTICE: A SYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMIZED CONTROLLED TRIALS
Henrotin, Yves ;
in Osteoarthritis and Cartilage (2015), 23(S2), 356Detailed reference viewed: 11 (0 ULg)
Curcuminoids Extract, Hydrolyzed Collagen and Green Tea Extract Synergically Inhibit Inflammatory and Catabolic Mediator's Synthesis by Normal Bovine and Osteoarthritic Human Chondrocytes in Monolayer
Comblain, Fanny ; Sanchez, Christelle ; et al
in PLoS ONE (2015)Detailed reference viewed: 11 (1 ULg)
Oleuropein or rutin consumption decreases the spontaneous development of osteoarthritis in the Hartley guinea pig.
; Sanchez, Christelle ; et al
in Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (2015), 23(1), 94-102
OBJECTIVE: To assess the potential protective effects of three polyphenols oleuropein, rutin and curcumin, on joint ageing and osteoarthritis (OA) development. DESIGN: Sixty 4-week-old Dunkin-Hartley ... [more ▼]
OBJECTIVE: To assess the potential protective effects of three polyphenols oleuropein, rutin and curcumin, on joint ageing and osteoarthritis (OA) development. DESIGN: Sixty 4-week-old Dunkin-Hartley guinea pigs were randomized into four groups and received daily during 31 weeks either standard guinea pig diet (control group) or a standard guinea pig diet enriched with oleuropein (0.025%), rutin (0.5%) or rutin/curcumin (0.5%/0.25%) association. Biomarkers of OA (Coll2-1, Coll2-1NO2, Fib3-1, Fib3-2, ARGS), as well as inflammation (PGE2) were quantified in the serum. Histological assessments of knee cartilage and synovial membrane were performed at week 4 (five young reference guinea pigs) and week 35. RESULTS: At week 35, guinea pigs in the control group spontaneously developed significant cartilage lesions with mild synovial inflammation. The histological scores of cartilage lesions and synovitis were well correlated with the increased level of serum biomarkers. Histologically, all treatments significantly reduced the cartilage degradation score (P < 0.01), but only oleuropein significantly decreased the synovial histological score (P < 0.05) and serum PGE2 levels (P < 0.01) compared to the control group. Coll2-1 was decreased by rutin and the combination of rutin/curcumin, Fib3-1 and Fib3-2 were only decreased by the rutin/curcumin mixture, while Coll2-1NO2 was significantly decreased by all treatments (P < 0.05). CONCLUSION: Oleuropein and rutin +/- curcumin significantly slowed down the progression of spontaneous OA lesions in guinea pigs. While no additive effect was seen in the curcumin + rutin group, the differential effects of oleuropein and rutin on inflammatory and cartilage catabolic markers suggest an interesting combination for future studies in OA protection. [less ▲]Detailed reference viewed: 30 (4 ULg)
Joint care composition
; Comblain, Fanny ; Henrotin, Yves
The present invention relates to a composition comprising curcuminoid with green tea polyphenol or with a combination of glycine, proline and hydroxyproline for use in preventing or treating ... [more ▼]
The present invention relates to a composition comprising curcuminoid with green tea polyphenol or with a combination of glycine, proline and hydroxyproline for use in preventing or treating osteoarthritis. It also relates to a method of preventing or treating osteoarthritis in mammals, the method comprising administering to said mammal a composition which comprises curcuminoid with green tea polyphenol or with a combination of glycine, proline and hydroxyproline. [less ▲]Detailed reference viewed: 11 (2 ULg)
Intra-articularly supplementation method for treating joint diseases and injuries
Henrotin, Yves ; Sanchez, Christelle ; Oprenyeszk, Frédéric et al
Patent (2014)Detailed reference viewed: 15 (1 ULg)
Cell cultivation in chitosan alginate hydrogel beads
Henrotin, Yves ; ; Sanchez, Christelle
Patent (2014)Detailed reference viewed: 13 (1 ULg)
Biomarker for osteoarthritis and/or other ageing-related diseases, and use thereof
Henrotin, Yves ; ; DEBERG, Michelle et al
Patent (2014)Detailed reference viewed: 18 (8 ULg)
Bone sialoprotein as a potential key factor implicated in the pathophysiology of osteoarthritis
Pesesse, Laurence ; Sanchez, Christelle ; et al
in Osteoarthritis and Cartilage (2014), 22(4), 547-56Detailed reference viewed: 12 (4 ULg)
Importance of synovitis in osteoarthritis: Evidence for the use of glycosaminoglycans against synovial inflammation.
Henrotin, Yves ; Lambert, Cécile ;
in Seminars in Arthritis & Rheumatism (2014), 43(5), 579-87Detailed reference viewed: 21 (3 ULg)
OARSI guidelines for the non-surgical management of knee osteoarthritis
; Henrotin, Yves
in Osteoarthritis and Cartilage (2014), 22(3), 363-388
This paper presents concise, up-to-date, patient-focused, evidence-based, expert consensus guidelines for the management of knee osteoarthritis, intended to inform patients, physicians and allied ... [more ▼]
This paper presents concise, up-to-date, patient-focused, evidence-based, expert consensus guidelines for the management of knee osteoarthritis, intended to inform patients, physicians and allied healthcare professionnal worlwide. [less ▲]Detailed reference viewed: 35 (1 ULg)
Targeting the synovial angiogenesis as a novel treatment approach to osteoarthritis.
Henrotin, Yves ; ; Lambert, Cécile
in Therapeutic Advances in Musculoskeletal Disease (2014), 6(1), 20-34Detailed reference viewed: 7 (0 ULg)
L'inhibition des voies de signalisation est-elle une possibilité thérapeutique pour l'arthrose?
in Revue du Rhumatisme (2014)
Les voies de signalisation sont des cibles intéressantes pour les traitements de l’arthrose car leur inhibition ou leur activation permet de réguler l’expression d’un ensemble de gènes cibles directement ... [more ▼]
Les voies de signalisation sont des cibles intéressantes pour les traitements de l’arthrose car leur inhibition ou leur activation permet de réguler l’expression d’un ensemble de gènes cibles directement impliqués dans le dysfonctionnement métabolique des tissus articulaires. Cependant, les premiers essais cliniques réalisés dans la PR ont montré que la régulation spécifique de la voie JAK/STAT induit de nombreux effets secondaires. Ce point pourrait être un facteur limitant l’utilisation d’inhibiteurs ou d’activateurs spécifiques des voies de signalisation dans l’arthrose. Dans cette maladie, le rapport risque/bénéfice doit rester le facteur clé de la décision thérapeutique, car il s’agit de traiter une maladie d’évolution lente, de sévérité modérée et associée à de nombreuses comorbidités. [less ▲]Detailed reference viewed: 19 (3 ULg)
Does signalling pathways inhibition hold therapeutic promise for osteoarthritis?
in Joint Bone Spine (2014), 81
Signalling pathways inhibition hold promise as therapeutic targets in osteoarthritis but safety concern may limit their use to the more sevre form of the disease.Detailed reference viewed: 18 (0 ULg)
What is the current statut of chondroitin sulfate and glucosamine in the treatment of knee osteoarthritis?
Henrotin, Yves ; ;
in Maturitas (2014), 78
Chondroitin sulfate and glucosamine sulfate exert beneficial effects on the metabolism of in vitro models of cells derived from synovial joints: chondrocytes, synoviocytes and cells from subchondral bone ... [more ▼]
Chondroitin sulfate and glucosamine sulfate exert beneficial effects on the metabolism of in vitro models of cells derived from synovial joints: chondrocytes, synoviocytes and cells from subchondral bone, all of which are involved in osteoarthritis (OA). They increase type II collagen and proteoglycan synthesis in human articular chondrocytes and are able to reduce the production of some pro-inflammatory mediators and proteases, to reduce the cellular death process, and improve the anabolic/catabolic balance of the extracellular cartilage matrix (ECM). Clinical trials have reported a beneficial effect of chondroitin sulfate and glucosamine sulfate on pain and function. The structure-modifying effects of these compounds have been reported and analyzed in recent meta-analyses. The results for knee OA demonstrate a small but significant reduction in the rate of joint space narrowing. Chondroitin sulfate and glucosamine sulphate are recommended by several guidelines from international societies for the management of knee and hip OA, while others do not recommend these products or recommend only under condition. This comprehensive review clarifies the role of these compounds in the therapeutic arsenal for patients with knee OA [less ▲]Detailed reference viewed: 24 (1 ULg)
Decrease of a specific biomarker of collagen degradation in osteoarthritis, Coll2-1, by treatment with highly bioavailable curcumin during an exploratory clinical trial.
; Henrotin, Yves
in BMC Complimentary & Alternative Medicine (2014)
The management of osteoarthritis (OA) remains a challenge. There is a need not only for safe and efficient treatments but also for accurate and reliable biomarkers that would help diagnosis and monitoring ... [more ▼]
The management of osteoarthritis (OA) remains a challenge. There is a need not only for safe and efficient treatments but also for accurate and reliable biomarkers that would help diagnosis and monitoring both disease activity and treatment efficacy. Curcumin is basically a spice that is known for its anti-inflammatory properties. In vitro studies suggest that curcumin could be beneficial for cartilage in OA. The aim of this exploratory, non-controlled clinical trial was to evaluate the effects of bio-optimized curcumin in knee OA patients on the serum levels of specific biomarkers of OA and on the evaluation of pain. This study highlighted the potential effect of curcumin in knee OA patient. This effect was reflected by the variation of a cartilage specific biomarker, Coll2-1 that was rapidly affected by the treatment. These results are encouraging for the qualification of Coll2-1 as a biomarker for the evaluation of curcumin in OA treatment. (Trial registration: NCT01909037 at clinicaltrials.gov) [less ▲]Detailed reference viewed: 30 (2 ULg)
Gene expression pattern of synovial cells from inflammatory and normal areas of osteoarthritis synovial membrane.
Lambert, Cécile ; ; et al
in Arthritis and Rheumatism (2014), sous presse
Objective: The aim of this study was to compare the gene expression pattern of synovial cells from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same ... [more ▼]
Objective: The aim of this study was to compare the gene expression pattern of synovial cells from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same osteoarthritis (OA) patient. Methods: Synovial tissues were obtained from 12 knee OA patients at the time of total knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria and sorted as N/R and I. Biopsies were cultured separately for 7 days. Microarray gene expression profiling between N/R and I areas was performed. Western blot and immunohistochemistry confirmed the identified genes that were differentially expressed. Results: 896 differentially expressed genes between N/R and I zones were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory network, TREM1 and S100A9 were strongly up-regulated. MMP-3 and -9, cathepsin H and S were significantly up-regulated in the cartilage catabolism pathway, whereas the most up-regulated anabolism enzyme was HAS1. Wnt-5A and LRP5 were up-regulated whereas FZD2 and DKK3 were down-regulated in the Wnt signaling. Finally, STC1, a protein involved in angiogenesis was identified as the most up-regulated gene in I zones compared to N/R zones. Conclusion: This study is the first to identify different expression pattern between two areas of the synovial membrane in the same patient. These differences concern several key pathways involved in OA pathogenesis. This analysis also provides information regarding new genes and proteins as potential targets for the future therapeutic. (c) 2013 American College of Rheumatology. [less ▲]Detailed reference viewed: 23 (4 ULg)
Oleuropein or rutin consumption decreases the spontaneous development of OA in Hartley guinea pig
Sanchez, Christelle ; ; et al
Conference (2013, November 23)Detailed reference viewed: 12 (4 ULg)