References of "Hayashi, T"
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See detailIdentification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC
Fakih, Ibrahim; Thiry, Damien ULg; Duprez, Jean-Noël ULg et al

in Veterinary Microbiology (2016)

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC ... [more ▼]

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www.genomicepidemiology.org). Of 233 isolates, 206 were triplex PCR-positive: 119 AE-STEC (58%), 78 EPEC (38%) and 9 STEC (4%); and the stx1+eae+ AE-STEC (49.5%) were the most frequent. Of them, 120 isolates (84% of AE-STEC, 23% of EPEC, 22% of STEC) tested positive with one O serogroup PCR: 57 for O26 (47.5%), 36 for O111 (30%), 10 for O103 (8%) and 8 for O5 (7%) serogroups. The analysis of the draft sequences of 15 O5 AE-STEC could not identify any difference correlated to the host. As a conclusion, (i) the AE-STEC associated with diarrhoea in young calves still belong to the same serogroups as previously (O5, O26, O111) but the O103 serogroup may be emerging, (ii) the O5 AE-STEC from calves and humans are genetically similar [less ▲]

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See detailPhylogenomic comparison of 18 clinical Escherichia coli strains belonging to serogroups O5 and O118 isolated from bovine and human
Taminiau, Bernard ULg; Ogura, Y.; Hayashi, T. et al

Conference (2014, October)

In developed countries enterohaemorrhagic Escherichia coli (EHEC) are responsible for small- or large-scale outbreaks of uncomplicated diarrhoea, haemorrhagic colitis and/or haemolytic–uraemic syndrome ... [more ▼]

In developed countries enterohaemorrhagic Escherichia coli (EHEC) are responsible for small- or large-scale outbreaks of uncomplicated diarrhoea, haemorrhagic colitis and/or haemolytic–uraemic syndrome (HUS) in human and cattle. If the two major EHEC serotypes in Europe well studied and characherized, several subdominant serotypes such as O5 and O118 are present in cattle and can act as a reservoir for potential outbreaks. This study present the phylogenomic comparison of 10 O118 and 8 O5 clinical E. coli strains that were isolated from cattle and human diarrhea. Genomic DNA was isolated from an active growing colony and sequenced on a MySeq apparatus. The raw sequences from the 18 genomes were assembled into scaffolds using an in-house pipeline and subjected to a quick automated annotation pipeline. The metabolic models of the strains were elaborated and compared. We used the contigs data to determine the MLST sequence types and virulotypes. A scheme of 20 houskeeping and virulence determinents were concatenated and used to select the most ancestral strains for both serotypes. This ancestral strain was selected for another run of sequencing and used as the anchor for a phylogenomic analysis of the 18 strains. The phylogenomic tree allowed us to analyse if the source parameter (human or bovine) could be used to cluster strains. This study shows that genomic sequencing has become a quick and efficient tool that can be used to replace numerous existing typing analysis methods by an unique analysis scheme. [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques ULg et al

Conference (2011, December)

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See detailTyping of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
Mainil, Jacques ULg; Bardiau, Marjorie ULg; Ooka, T. et al

in Journal of Applied Microbiology (2011), 111(3), 773-86

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS ... [more ▼]

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS AND RESULTS: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. CONCLUSIONS: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. SIGNIFICANCE AND IMPACT OF THE STUDY: The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks. [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, June)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, March)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, March)

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