La physiologie des interactions entre la pomme de terre et les pucerons : vers une nouvelle stratégie de lutte basée sur les systèmes de défense de la plante
Harmel, Nicolas ; Francis, Frédéric ; Haubruge, Eric et al
in Cahiers Agricultures (2008), 17Detailed reference viewed: 72 (17 ULg)
Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae.
Harmel, Nicolas ; ; et al
in Insect Molecular Biology (2008), 17(2), 165-74
The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence ... [more ▼]
The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence reactions. Here, we focused on the salivary proteome of the green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or were separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides were then identified by mass spectrometry coupled with database investigations. A homemade database was constituted of expressed sequence tags from the pea aphid Acyrtosiphon pisum and M. persicae. The databases were used to identify proteins related to M. persicae with a nonsequenced genome. This procedure enabled us to discover glucose oxidase, glucose dehydrogenase, NADH dehydrogenase, alpha-glucosidase and alpha-amylase in M. persicae saliva. The presence of these enzymes is discussed in terms of plant-aphid interactions. [less ▲]Detailed reference viewed: 74 (24 ULg)
Role Of Terpenes From Aphid-Infested Potato On Searching And Oviposition Behavior Of Episyrphus Balteatus
Harmel, Nicolas ; ; Fauconnier, Marie-Laure et al
in Insect Science (2007), 14(1),Detailed reference viewed: 33 (10 ULg)
Proteome variations of the Myzus persicae aphid according to host plant change.
Francis, Frédéric ; ; Harmel, Nicolas et al
in Insect Biochemistry and Molecular Biology (2006), 36(3), 219-227Detailed reference viewed: 35 (9 ULg)
Proteomics in Myzus persicae: effect of aphid host plant switch.
Francis, Frédéric ; ; Harmel, Nicolas et al
in Insect Biochemistry and Molecular Biology (2006), 36(3), 219-27
Chemical ecology is the study of how particular chemicals are involved in interactions of organisms with each other and with their surroundings. In order to reduce insect attack, plants have evolved a ... [more ▼]
Chemical ecology is the study of how particular chemicals are involved in interactions of organisms with each other and with their surroundings. In order to reduce insect attack, plants have evolved a variety of defence mechanisms, both constitutive and inducible, while insects have evolved strategies to overcome these plant defences (such as detoxification enzymes). A major determinant of the influence of evolutionary arms races is the strategy of the insect: generalist insect herbivores, such as Myzus persicae aphid, need more complex adaptive mechanisms since they need to respond to a large array of different plant defensive chemicals. Here we studied the chemical ecology of M. persicae associated with different plant species, from Brassicaceae and Solanaceae families. To identify the involved adaptation systems to cope with the plant secondary substances and to assess the differential expression of these systems, a proteomic approach was developed. A non-restrictive approach was developed to identify all the potential adaptation systems toward the secondary metabolites from host plants. The complex protein mixtures were separated by two-dimensional electrophoresis methods and the related spots of proteins significantly varying were selected and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Fourteen aphid proteins were found to vary according to host plant switch; ten of them were down regulated (proteins involved in glycolysis, TCA cycle, protein and lipid synthesis) while four others were overexpressed (mainly related to the cytoskeleton). These techniques are very reliable to describe the proteome from organisms such as insects in response to particular environmental change such as host plant species of herbivores. [less ▲]Detailed reference viewed: 62 (8 ULg)