References of "Hanikenne, Marc"
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See detailMetal homeostasis in plant mitochondria
Vigani, Gianpiero; Hanikenne, Marc ULiege

in Logan, David C (Ed.) Plant Mitochondria, 2d edition (2018)

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See detailPhysiology and molecular biology of trace element hyperaccumulation
Merlot, Sylvain; Garcia de la Torre, Vanesa S.; Hanikenne, Marc ULiege

in Echevarria, Guillaume; van der Ent, Antony; Morel, Jean Louis (Eds.) et al Agromining: farming for metals. Extracting unconventional resources using plants. (2018)

Detailed reference viewed: 32 (1 ULiège)
See detailThe Arabidopsis frd3 mutant phenotype: a new level of complexity
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2017, December 07)

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See detailIn vivo chlorophyll fluorescence screening allows the isolation of a Chlamydomonas mutant defective for NDUFAF3, an assembly factor involved in mitochondrial complex I assembly
Massoz, Simon; Hanikenne, Marc ULiege; Bailleul, Benjamin et al

in The Plant Journal (2017), 17(8), 2045-2054

The qualitative screening method to select complex I mutants in the microalga Chlamydomonas, based on reduced growth under heterotrophic condition, is not suited for high throughput screening. In order to ... [more ▼]

The qualitative screening method to select complex I mutants in the microalga Chlamydomonas, based on reduced growth under heterotrophic condition, is not suited for high throughput screening. In order to develop a fast screening method based on measurements of chlorophyll fluorescence, we first demonstrated that complex I mutants displayed decreased photosystem II efficiency in the genetic background of a photosynthetic mutation leading to reduced formation of the electrochemical proton gradient in the chloroplast (pgrl1 mutation). In contrast, single mutants (complex I and pgrl1 mutants) could not be distinguished from wild type by their photosystem II efficiency in the tested conditions. We next performed an insertional mutagenesis on the pgrl1 mutant. Out of ~3000 hygromycin-resistant insertional transformants, 46 had decreased photosystem II efficiency and three were complex I mutants. One of the mutants was tagged and whole genome sequencing identified the resistance cassette in NDUFAF3, a homolog of the human NDUFAF3 gene, encoding for an assembly factor involved in complex I assembly. Complemented strains showed restored complex I activity and assembly. Overall, we described here a screening method which is fast and particularly suited for identification of Chlamydomonas complex I mutants. [less ▲]

See detailFunctional and structural characterisation of the Arabidopsis thaliana HMA4 protein
Lekeux, Gilles ULiege; Laurent, Clémentine; Xiao, Zhiguang et al

Poster (2017, July 24)

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See detailIntraspecific variation of Zn hyperaccumulation in Arabidopsis halleri populations
Schvartzman Echenique, Maria Sol ULiege; Corso, Massimiliano; Fataftah, Nazeer ULiege et al

Conference (2017, July 19)

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See detailComparative Proteomics Analysis Provides New Candidates for Zinc Homeostasis Regulation in Arabidopsis
Amini, Sahand ULiege; Arsova, Borjana ULiege; Scheepers, Maxime ULiege et al

Poster (2017, July 03)

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants ... [more ▼]

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants at high concentrations in soils. This calls for a better unravelling of Zn homeostasis regulation mechanisms, including sensing and signaling in plants. In order to fulfill this aim, we are testing for novel proteins involved in Zn homeostasis in the model plant Arabidopsis thaliana. First, quantitative proteomics was performed on root and shoot samples obtained upon Zn starvation and re-supply in different spatio-temporal conditions. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was also performed for those treatments to measure the Zn concentration in tissues. It showed very rapid Zn uptake in root upon re-supply. Moreover, quantitative expression studies of known players of Zn homeostasis confirmed our large-scale proteomic results, although for a few genes lack of correlation between transcript and protein regulation was observed. Using clustering, statistical and gene ontology analyses, we selected candidate genes for further studies. Among more than 5000 detected proteins in roots by shotgun proteomics, 75 genes were selected for targeted analyses. In general, our results show that comparative proteomics study can be useful to reveal new players in the Zn regulatory network in plants, which can lead to new Zn biofortification and phytoremediation strategies. [less ▲]

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See detailStreptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
Martinet, Loïc ULiege; Maciejewska, Marta; Adam, Delphine ULiege et al

Poster (2017, May 24)

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical ... [more ▼]

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials. Phylogenetic analysis revealed that all 78 isolates were exclusively affiliated to the genus Streptomyces and clustered into 31 distinct phylotypes displaying various pigmentation patterns and morphological features. Phylotype representatives were tested for antibacterial and antifungal activities and their genomes were mined for secondary metabolite biosynthetic genes coding for non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKS). The moonmilk Streptomyces collection was found to display strong inhibitory activities against a wide range of reference organisms, as 94, 71, and 94% of the isolates inhibited or impaired the growth of Gram-positive, Gram-negative bacteria, and fungi, respectively. Interestingly, 90% of the cave strains induced strong growth suppression against the multi-drug resistant Rasamsonia argillacea, a causative agent of invasive mycosis in cystic fibrosis and chronic granulomatous diseases. We are currently identifying molecules responsible for the observed antimicrobial activities which, next to few known bioactive compounds, also reveals many active compounds with molecular masses that do not correspond to known antibiotic deposited in web biomolecules databases such as KNapSAcK, Pubchem, Chemspider. The challenge is to increase the production yields of these unknown compounds, and purify them to further characterize their structure by NMR. Overall, our work supports the common belief that moonmilk might effectively treat various infectious diseases thanks to the presence of a highly diverse population of prolific antimicrobial producing Streptomyces, and thus may indeed constitute a promising reservoir of potentially novel active natural compounds. [less ▲]

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See detailZinc, manganese and iron interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Goormaghtigh, Erik et al

Poster (2017, February 01)

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See detailIron, zinc and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Goormaghtigh, Erik et al

Poster (2017, January 12)

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See detailAssessment of the Potential Role of Streptomyces in Cave Moonmilk Formation
Maciejewska, Marta; Adam, Delphine; Naomé, Aymeric ULiege et al

in Frontiers in Microbiology (2017), 8

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