References of "Hanikenne, Marc"
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See detailPhysiology and molecular biology of trace element hyperaccumulation
Merlot, Sylvain; Garcia de la Torre, Vanesa S.; Hanikenne, Marc ULg

in Echevarria, Guillaume; van der Ent, Antony; Morel, Jean Louis (Eds.) et al Agromining: farming for metals. Extracting unconventional resources using plants. (in press)

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See detailChapter 5. Metal homeostasis in plant mitochondria
Vigani, Gianpiero; Hanikenne, Marc ULg

in Logan, David C (Ed.) Plant Mitochondria, a volume in the Annual Plant Reviews series (in press)

Detailed reference viewed: 68 (5 ULg)
See detailFunctional and structural characterisation of the Arabidopsis thaliana HMA4 protein
Lekeux, Gilles ULg; Laurent, Clémentine; Xiao, Zhiguang et al

Poster (2017, July 24)

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See detailIntraspecific variation of Zn hyperaccumulation in Arabidopsis halleri populations
Schvartzman Echenique, Maria Sol ULg; Corso, Massimiliano; Fataftah, Nazeer ULg et al

Conference (2017, July 19)

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See detailComparative Proteomics Analysis Provides New Candidates for Zinc Homeostasis Regulation in Arabidopsis
Amini, Sahand ULg; Arsova, Borjana ULg; Scheepers, Maxime ULg et al

Poster (2017, July 03)

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants ... [more ▼]

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants at high concentrations in soils. This calls for a better unravelling of Zn homeostasis regulation mechanisms, including sensing and signaling in plants. In order to fulfill this aim, we are testing for novel proteins involved in Zn homeostasis in the model plant Arabidopsis thaliana. First, quantitative proteomics was performed on root and shoot samples obtained upon Zn starvation and re-supply in different spatio-temporal conditions. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was also performed for those treatments to measure the Zn concentration in tissues. It showed very rapid Zn uptake in root upon re-supply. Moreover, quantitative expression studies of known players of Zn homeostasis confirmed our large-scale proteomic results, although for a few genes lack of correlation between transcript and protein regulation was observed. Using clustering, statistical and gene ontology analyses, we selected candidate genes for further studies. Among more than 5000 detected proteins in roots by shotgun proteomics, 75 genes were selected for targeted analyses. In general, our results show that comparative proteomics study can be useful to reveal new players in the Zn regulatory network in plants, which can lead to new Zn biofortification and phytoremediation strategies. [less ▲]

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See detailStreptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
Martinet, Loïc ULg; Maciejewska, Marta; Adam, Delphine ULg et al

Poster (2017, May 24)

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical ... [more ▼]

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials. Phylogenetic analysis revealed that all 78 isolates were exclusively affiliated to the genus Streptomyces and clustered into 31 distinct phylotypes displaying various pigmentation patterns and morphological features. Phylotype representatives were tested for antibacterial and antifungal activities and their genomes were mined for secondary metabolite biosynthetic genes coding for non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKS). The moonmilk Streptomyces collection was found to display strong inhibitory activities against a wide range of reference organisms, as 94, 71, and 94% of the isolates inhibited or impaired the growth of Gram-positive, Gram-negative bacteria, and fungi, respectively. Interestingly, 90% of the cave strains induced strong growth suppression against the multi-drug resistant Rasamsonia argillacea, a causative agent of invasive mycosis in cystic fibrosis and chronic granulomatous diseases. We are currently identifying molecules responsible for the observed antimicrobial activities which, next to few known bioactive compounds, also reveals many active compounds with molecular masses that do not correspond to known antibiotic deposited in web biomolecules databases such as KNapSAcK, Pubchem, Chemspider. The challenge is to increase the production yields of these unknown compounds, and purify them to further characterize their structure by NMR. Overall, our work supports the common belief that moonmilk might effectively treat various infectious diseases thanks to the presence of a highly diverse population of prolific antimicrobial producing Streptomyces, and thus may indeed constitute a promising reservoir of potentially novel active natural compounds. [less ▲]

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See detailZinc, manganese and iron interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2017, February 01)

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See detailIron, zinc and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2017, January 12)

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See detailAssessment of the Potential Role of Streptomyces in Cave Moonmilk Formation
Maciejewska, Marta; Adam, Delphine; Naomé, Aymeric ULg et al

in Frontiers in Microbiology (2017), 8

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See detailZinc, iron and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2016, November 16)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). [less ▲]

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See detailPREDetector 2.0: Online and Enhanced Version of the Prokaryotic Regulatory Elements Detector Tool
Tocquin, Pierre ULg; Naomé, Aymeric ULg; Jourdan, Samuel ULg et al

E-print/Working paper (2016)

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their ... [more ▼]

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their expression. To this end, we have developed an improved and online version of the PREDetector software aimed at identifying putative transcription factor-binding sites (TFBS) in bacterial genomes. The original philosophy of PREDetector 1.0 is maintained, i.e. to allow users to freely fix the DNA-motif screening parameters, and to provide a statistical means to estimate the reliability of the prediction output. This new version offers an interactive table as well as graphics to dynamically alter the main screening parameters with automatic update of the list of identified putative TFBS. PREDetector 2.0 also has the following additional options: (i) access to genome sequences from different databases, (ii) access to weight matrices from public repositories, (iii) visualization of the predicted hits in their genomic context, (iv) grouping of hits identified in the same upstream region, (v) possibility to store the performed jobs, and (vi) automated export of the results in various formats. PREDetector 2.0 is available at http://predetector.fsc.ulg.ac.be/. [less ▲]

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See detailGenomics and ecological adaptation: metal hyperaccumulation in Arabidopsis halleri
Hanikenne, Marc ULg

Conference (2016, September 27)

Detailed reference viewed: 41 (6 ULg)