References of "Hanikenne, Marc"
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See detailChapter 5. Metal homeostasis in plant mitochondria
Vigani, Gianpiero; Hanikenne, Marc ULg

in Logan, David C (Ed.) Plant Mitochondria, a volume in the Annual Plant Reviews series (in press)

Detailed reference viewed: 56 (4 ULg)
See detailZinc, iron and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2016, November 16)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). [less ▲]

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See detailPREDetector 2.0: Online and Enhanced Version of the Prokaryotic Regulatory Elements Detector Tool
Tocquin, Pierre ULg; Naomé, Aymeric ULg; Jourdan, Samuel ULg et al

in bioRxiv (2016)

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their ... [more ▼]

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their expression. To this end, we have developed an improved and online version of the PREDetector software aimed at identifying putative transcription factor-binding sites (TFBS) in bacterial genomes. The original philosophy of PREDetector 1.0 is maintained, i.e. to allow users to freely fix the DNA-motif screening parameters, and to provide a statistical means to estimate the reliability of the prediction output. This new version offers an interactive table as well as graphics to dynamically alter the main screening parameters with automatic update of the list of identified putative TFBS. PREDetector 2.0 also has the following additional options: (i) access to genome sequences from different databases, (ii) access to weight matrices from public repositories, (iii) visualization of the predicted hits in their genomic context, (iv) grouping of hits identified in the same upstream region, (v) possibility to store the performed jobs, and (vi) automated export of the results in various formats. PREDetector 2.0 is available at http://predetector.fsc.ulg.ac.be/. [less ▲]

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See detailGenomics and ecological adaptation: metal hyperaccumulation in Arabidopsis halleri
Hanikenne, Marc ULg

Conference (2016, September 27)

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See detailCharacterization of FRD3, a zinc and iron homeostasis actor in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean Benoit; Spielmann, Julien ULg et al

Conference (2016, July 14)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). We are further examining the FRD3 function in zinc and iron homeostasis in A. thaliana and A. halleri. We will present data (i) on the high expression of FRD3 in A. halleri, (ii) on the functional characterization of the two alternative FRD3 transcripts and their role in metal homeostasis in A. thaliana in comparison with the A. halleri FRD3 transcript and (iii) on the zinc phenotypes of the frd3 A. thaliana mutant. [less ▲]

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See detailDiversity and evolution of transposable elements in the Arabidopsis lyrata and A. halleri genomes
Caron, Thibault; Legrand, Sylvain; Schvartzman Echenique, Maria Sol ULg et al

Conference (2016, July 07)

Detailed reference viewed: 36 (5 ULg)
See detailCharacterization of FRD3, a iron and zinc homeostasis actor in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit; Spielmann, Julien ULg et al

Poster (2016, June 09)

Detailed reference viewed: 25 (3 ULg)
See detailZinc and Iron interplay in Arabidopsis relatives
Hanikenne, Marc ULg

Conference (2016, May 24)

Detailed reference viewed: 14 (1 ULg)
See detailCharacterization of FRD3, a zinc and iron homeostasis actor, in Arabidopsis
Scheepers, Maxime ULg; Charlier, Jean-Benoit; Spielmann, Julien ULg et al

Poster (2016, May 13)

Detailed reference viewed: 35 (5 ULg)
See detailZinc and Iron interplay in Arabidopsis relatives
Hanikenne, Marc ULg

Scientific conference (2016, March 25)

Detailed reference viewed: 15 (3 ULg)
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See detailDynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors
Stankovic, Nancy ULg; Schloesser, Marie ULg; Joris, Marine ULg et al

in Plant Physiology (2016), 170

Serine/Arginine-rich (SR) proteins are essential nucleus-localized splicing factors. Our prior studies showed that Arabidopsis RSZ22, a homolog of the human SRSF7 SR factor, exits the nucleus through two ... [more ▼]

Serine/Arginine-rich (SR) proteins are essential nucleus-localized splicing factors. Our prior studies showed that Arabidopsis RSZ22, a homolog of the human SRSF7 SR factor, exits the nucleus through two pathways, either dependent or independent on the XPO1 receptor. Here, we examined the expression profiles and shuttling dynamics of the Arabidopsis SRSF1 subfamily (SR30, SR34, SR34a and SR34b) under control of their endogenous promoter in Arabidopsis and in transient expression assay. Due to its rapid nucleocytoplasmic shuttling and high expression level in transient assay, we analysed the multiple determinants that regulate the localisation and shuttling dynamics of SR34. By site-directed mutagenesis of SR34 RNA-binding sequences and RS domain, we further show that functional RRM1 or RRM2 are dispensable for the exclusive protein nuclear localization and speckle-like distribution. However, mutations of both RRMs induced aggregation of the protein whereas mutation in the RS domain decreased the stability of the protein and suppressed its nuclear accumulation. Furthermore, the RNA-binding motif mutants are defective for their export through the XPO1 (CRM1/Exportin-1) receptor pathway, but retain nucleocytoplasmic mobility. We performed a yeast two hybrid screen with SR34 as bait and discovered SR45 as a new interactor. SR45 is an unusual SR splicing factor bearing two RS domains. These interactions were confirmed in planta by FLIM-FRET and BiFC and the roles of SR34 domains in protein-protein interactions were further studied. Altogether, our report extends our understanding of shuttling dynamics of Arabidopsis SR splicing factors [less ▲]

Detailed reference viewed: 64 (26 ULg)