References of "Hance, Thierry"
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See detailInfluence of the secondary endosymbiont Serratia Symbiotica on the resistance to the parasitism in the aphid Acyrthosiphon pisum
Attia, Sabrine; Foray, Vincent; Louâpre, Philippe et al

in Journal of Entomology and Zoology Studies (in press)

Aphids have an obligate association with the primary symbiont Buchnera aphidicola and is known to affect aphid fitness. Aphids commonly harbour other facultative bacterial endosymbionts and may benefit ... [more ▼]

Aphids have an obligate association with the primary symbiont Buchnera aphidicola and is known to affect aphid fitness. Aphids commonly harbour other facultative bacterial endosymbionts and may benefit from their presence through increased resistance to parasitoids [1]. Present results suggest that the ability of hosts to defend against natural enemies depend not only on the presence of symbionts but also on the host genotype. Aphids represent a complex micro-environment for the parasitoid larvae and both the aphid physiology and endosymbiont influence their survival until mummification. [less ▲]

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See detailInsecticidal activity of Lavandula angustifolia Mill. against the pea aphid Acyrtosiphum pisum
Attia, Sabrine; Lognay, Georges ULg; Heuskin, Stéphanie ULg et al

in Journal of Entomology and Zoology Studies (2016), 4(1), 118-122

The essential oil of Lavandula angustifolia was investigated for its biocide activity against Acyrtosiphum pisum by fumigation. The oil was characterised by GC-MS revealing that linalool was the most ... [more ▼]

The essential oil of Lavandula angustifolia was investigated for its biocide activity against Acyrtosiphum pisum by fumigation. The oil was characterised by GC-MS revealing that linalool was the most abundant component (38.57%), followed by linalyl acetate (29.95%), 1,8-cineole (13,66%), camphor (13,13%), -pinene (3,14%) and terpinene-4-ol (1,54%). The Mortality was measured upon treatment with oil concentrations ranging from 5 to 25 µl.l-1 of air. The mortality of aphids increased with oil concentration and LC50 values were determined to be 11.2 µl.l-1 of air. The full mixture was also prepared and toxicity was compared with individual constituents. The results showed that the presence of all constituents were necessary to equal the toxicity of the natural oils L. angustifolia oil can provide valuable pesticide activity with significantly lower LC50 values [less ▲]

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See detailAn analysis of potential resistance of the phytophagous mite, Tetranychus urticae Koch (Acari: Tetranychidae) to four botanical pesticides
Attia, Sabrine; Lebdi, KL; Heuskin, Stéphanie ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2015), 19(3), 232-238

Description of the subject. Synthetic acaricides have been widely used to manage Tetranychus urticae. Due to the excessive use of biocide and the associated problems of pesticide resistance and ... [more ▼]

Description of the subject. Synthetic acaricides have been widely used to manage Tetranychus urticae. Due to the excessive use of biocide and the associated problems of pesticide resistance and environmental pollution, there is an increasing demand for sustainable, environmentally-friendly control methods. Among the current alternative strategies aimed at decreasing the pest populations, the pesticides based on plant extracts are currently one of the most promising methods. Essential oils with acaricidal properties have been categorized as green pesticides because they are biodegradable and predominantly non-toxic to vertebrates. Objectives. With an aim to reduce the use of synthetic pesticides, they represent a promising approach for eco-chemical control of mites. Method. The aim of the present work was to analyze the risk of resistance emergence of T. urticae to repeated treatments with four plant extracts: Deverra scoparia Coss. & Durieu (Araliales: Apiaceae), Hertia cheirifolia (L.) Kuntze (Asterales: Ateraceae), Santolina africana Jord. & Fourr. (Asterales: Asteraceae) essential oils and garlic distillate Allium sativum L. (Asparagales: Alliaceae) after 20 generations. Results. Repeated treatments with S. africana essential oil during 20 generations did not provoke an emergence of resistance while a low development of resistance was observed with H. cheirifolia, A. sativum and D. scoparia extracts. Conclusions. The efficacy of these extracts against the two spotted spider mite and their low development of resistance make them a promising use for pest management. [less ▲]

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See detailLa carte blanche : Enjeux de la Politique agricole commune en Wallonie : une volonté de découpler nature et agriculture ?
Jacquemart, Anne-Laure; Dendoncker, Nicolas; Dufrêne, Marc ULg et al

Article for general public (2014)

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See detailTechnologie de production de masse d’insectes - INSECTECH
Richard, Gaetan ULg; Hance, Thierry; Fauconnier, Marie-Laure ULg et al

Report (2014)

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See detailAcar'up, a new trapping device for house dust mites
Mailleux, Anne-Catherine; Deneubourg, Jean-Louis; Detrain, Claire et al

Poster (2014)

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See detailHow to get rid of the two spotted spider mites?
Attia, Sabrine; Grissa, Kaouthar Lebdi; Heuskin, Stéphanie ULg et al

in Revue des Régions Arides (2014), 35(3), 1739-1748

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion ... [more ▼]

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion bioassay without allowing direct contact. Responses varied according to oil type and dose, and mite species. The chemical analyses with GC-MS and GC-FID revealed that the two oils differed in their most abundant components. The most abundant components in the Citrus oil were linalyl acetate (41.95%), sabinene (18.60%) and linalool (18.14%) whereas pulegone (41.86%) and menthone (28.33%) were most prevalent in the Mentha oil. Mortality and fecundity were measured with 15 oils concentrations ranged from 0.01 to 8μl/l of air. Tetranychid mortality increased with increasing concentrations with LC50 value of 5.39 and 4.09 μl/l for C. aurantium and M. pulegium, respectively. However few mortality was observed in the case of P. persimilis with LC50 value of 0.46 and 0.26 μl/l for C. aurantium and M. pulegium respectively. For both oils a reduction of fecundity was observed at 0.01 μ/l in the case of T. urticae. The essential oils described herein have potential interest as fumigants for the bio-control of T. urticae. [less ▲]

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See detailWhole-genome sequence of Serratia symbiotica strain CWBI-2.3T, a free-living symbiont of the Black Bean Aphid Aphis fabae
Foray, Vincent; Grigorescu, Alina ULg; Sabri, Ahmed et al

in Genome Announcements (2014), 2(4),

The gammaproteobacterium Serratia symbiotica is one of the major secondary symbionts found in aphids. Here, we report the draft genome sequence of S. symbiotica strain CWBI-2.3T, previously isolated from ... [more ▼]

The gammaproteobacterium Serratia symbiotica is one of the major secondary symbionts found in aphids. Here, we report the draft genome sequence of S. symbiotica strain CWBI-2.3T, previously isolated from the black bean aphid Aphis fabae. The 3.58-Mb genome sequence might provide new insights to understand the evolution of insect-microbe symbiosis. [less ▲]

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See detailThe facultative bacterial symbiont Serratia symbiotica in Acyrtosiphon pisum confer resistance to Aphidius ervi
Attia, Sabrine; Foray, Vincent; Louâpres, Philippe et al

Poster (2014)

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See detailDoes the facultative bacteria Serratia symbiotica influence the foraging strategies of aphid parasitoids?
Attia, Sabrine; Louâpre, Philippe; Foray, Vincent et al

Poster (2014)

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See detailTechnologie de production de masse d’insectes - INSECTECH
Richard, Gaetan ULg; Hance, Thierry; Fauconnier, Marie-Laure ULg et al

Report (2013)

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See detailProteomic Investigation of Aphid Honeydew Reveals an Unexpected Diversity of Proteins
Sabri, Ahmed; Vandermoten, Sophie ULg; Leroy, Pascal et al

in PLoS ONE (2013), 8(9),

Aphids feed on the phloem sap of plants, and are the most common honeydew-producing insects. While aphid honeydew is primarily considered to comprise sugars and amino acids, its protein diversity has yet ... [more ▼]

Aphids feed on the phloem sap of plants, and are the most common honeydew-producing insects. While aphid honeydew is primarily considered to comprise sugars and amino acids, its protein diversity has yet to be documented. Here, we report on the investigation of the honeydew proteome from the pea aphid Acyrthosiphon pisum. Using a two-Dimensional <br />Differential in-Gel Electrophoresis (2D-Dige) approach, more than 140 spots were isolated, demonstrating that aphid honeydew also represents a diverse source of proteins. About 66% of the isolated spots were identified through mass spectrometry analysis, revealing that the protein diversity of aphid honeydew originates from several organisms (i.e. the host aphid and its microbiota, including endosymbiotic bacteria and gut flora). Interestingly, our experiments also allowed to identify some proteins like chaperonin, GroEL and Dnak chaperones, elongation factor Tu (EF-Tu), and flagellin that might act as mediators in the plant-aphid interaction. In addition to providing the first aphid honeydew proteome analysis, we propose to reconsider the importance of this substance, mainly acknowledged to be a waste product, from the aphid <br />ecology perspective. [less ▲]

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See detailFumigant toxicity of Mentha pulegium and Citrus aurantium essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae)
Attia, Sabrine; Grissa, K.L.; Lognay, Georges ULg et al

in Boeckx; Bossier, Peter; Smagghe, Guy (Eds.) et al Proceedings of the 65th international symposium on crop protection (2013, May 21)

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See detailCompte-rendu et apport de la conférence-débat : « Nature, conditionnalité et verdissement de la PAC : un tournant wallon ? ». 9 novembre 2011. Espace Senghor, Gembloux, Belgique.
Piqueray, Julien ULg; Richard, Catherine ULg; Walot, Thierri et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2013), 17

Recently, the European Union (EU) proposed a reform project for the Common Agricultural Policy (CAP). One of the proposed reforms consists in imposing to set aside 7% of arable land for biodiversity ... [more ▼]

Recently, the European Union (EU) proposed a reform project for the Common Agricultural Policy (CAP). One of the proposed reforms consists in imposing to set aside 7% of arable land for biodiversity support purposes. About this proposal, a conference was hold in Gembloux, Belgium, on 9th November 2011. Such systems already exist in neighboring countries. French and Swiss systems were displayed in order to highlight their advantages and limits. Scientific arguments subtending such policies were presented. It was shown that creation of semi-natural elements in the agricultural matrix should be efficient to promote biodiversity. Both the quantity and the quality of these elements should be considered. Also, biodiversity is likely to provide services to agriculture, notably crop pollination and pest control. Currently, uncertainties remain on eligible areas to reach the 7% threshold. However, based on most probable scenarios, the current likely eligible areas cover only 4.3% of arable land in Wallonia. Less than 20% of Walloon farms already reach the 7% threshold proposed by the EU. If the European project is approved, it will therefore be of first importance to have a societal recognition toward farmers for their effort. To optimize positive effects of the policy on biodiversity, it is also imperative to make sure that a high biological quality is reached in the areas dedicated to biodiversity and to monitor this quality in the light of clearly defined objectives. Economical, societal and environmental issues should all be considered when applying this policy in order to improve agriculture sustainability and to stop biodiversity decline in agricultural landscapes. [less ▲]

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See detailAcaricidal activities of Santolina africana and Hertia cheirifolia essential oils against the two-spotted spidermite (Tetranychus urticae)
Attia, Sabrine; Grissa, K.L.; Mailleux, A-C et al

in Pest Management Science (2012)

BACKGROUND: Many plant essential oils show a broad spectrum of activity against pests. This study investigated the effects of two essential oils on Tetranychus urticae, one of the most serious pests in ... [more ▼]

BACKGROUND: Many plant essential oils show a broad spectrum of activity against pests. This study investigated the effects of two essential oils on Tetranychus urticae, one of the most serious pests in the world. RESULTS: The chemical composition of the two oils was characterised by GC-MS. The most abundant component in the Santolina africana (Jord. & Fourr) oil was terpinen-4-ol (54.96%), while thymol (61%) was prevalent in the Hertia cheirifolia (L.) oil. Mortality and fecundity were measured upon treatment with oil concentrations ranging from 0.07 to 6.75 mg L-1 with a Potter spray tower.Mite mortality increased with oil concentration, with LC50 values of 2.35 mg L-1 for S. africana and 3.43mg L-1 for H. cheirifolia respectively. For both oils, a reduction in fecundity was observed at concentrations of 0.07, 0.09 and 0.29mg L-1. Artificial blends of constituents of oils were also prepared and tested with individual constituents missing from the mixture. The results showed that the presence of all constituents was necessary to equal the toxicity of the two natural oils. CONCLUSION: S. africana and H. cheirifolia oils can provide valuable acaricide activitywith significantly lower LC50 values. Thus, these oils cause important mortality and reduce the number of eggs laid by females. [less ▲]

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See detailL’agroécologie : trajectoire et potentiel Pour une transition vers des systèmes alimentaires durables
Stassart, Pierre M ULg; Baret, Philippe; Grégoire, Jean-Claude et al

in Vandam, Denise; Streith, Michel; Nizet, Jean (Eds.) et al Agroéocologie, entre pratiques et sciences sociales (2012)

The aim of this paper is to define a conceptual framework for agroecology. There are two components to our approach. The first one proposes an analysis of what agroecology encompasses today, how its ... [more ▼]

The aim of this paper is to define a conceptual framework for agroecology. There are two components to our approach. The first one proposes an analysis of what agroecology encompasses today, how its definition evolved, and in which sociohistorical contexts it emerged and is currently developing. The second one sets the outlines of agroecology’s interdisciplinary approach. This entails a set of principles that should guide research in support of the development of agroecology and a series of medium-term prospects for research in the European context. This paper’s ultimate aim is to set a working framework. As such, it is also an invitation to have the various scientific communities concerned by the subject take part in dialogue with each other, with the agricultural professions and public administrations so as to allow for the diversity of practices involved, and with civil society and political circles. [less ▲]

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See detailOptimisation of a semiochemical slow-release alginate formulation attractive towards Aphidius ervi Haliday parasitoids
Heuskin, Stéphanie ULg; Lorge, Stéphanie; Godin, Bruno et al

in Pest Management Science (2011)

BACKGROUND: Optimisation of alginate formulations is described in order to develop semiochemical (E-β-farnesene and E-β-caryophyllene) slow-release devices in biological control approaches by attracting ... [more ▼]

BACKGROUND: Optimisation of alginate formulations is described in order to develop semiochemical (E-β-farnesene and E-β-caryophyllene) slow-release devices in biological control approaches by attracting predators and parasitoids of aphids. Various formulation criteria were optimised with respect to semiochemical encapsulation capacity. Moreover, the optimised formulation was characterised by texturometry and confocal microscopy. The slow-release rates of semiochemicals were calculated in laboratory controlled conditions. The attractiveness of semiochemical formulations towards Aphidius ervi was demonstrated by olfactometry. RESULTS: Two major parameters were highlighted in encapsulation optimisation: the type of alginate (Sigma L) and the type of crosslinker ion (Ca2+). Other formulation parameters were optimised: ionic strength (0.5M), Ca2+ (0.2 M) and alginate (1.5%) concentrations and the maturation time of beads in CaCl2 solution (48 h). After physical characterisation of beads, semiochemical slow-release measurements showed that alginate formulations were efficient sesquiterpene releasers, with 503 μg of E-β-farnesene and 1791 μg of E-β-caryophyllene totally released in 35 days. The efficiency of semiochemical alginate beads as attractants for female parasitoids was demonstrated, with high percentages of attraction for semiochemical odours (88 and 90% for E-β-farnesene and E-β-caryophyllene respectively) and significant statistical results. CONCLUSION: Semiochemical alginate beads can be considered as efficient slow-release systems in biological control. These formulations could be very useful to attract aphid parasitoids on crop fields. [less ▲]

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See detailAcaricidal activities of Santolina Africana and Hertia Cheirifolia, two endemic plants of north Africa, on the two spotted spider mite (Tetranychus urticae)
Attia, Sabrine; Grissa, Kaouthar Lebdi; Le Goff, Guillaume et al

Poster (2011, May)

The two-spotted spider mite, Tetranychus urticae Koch, is a worldwide pest which feeds on a large variety of plant families. Because its resistance to acaricides is spreading rapidly, the development of ... [more ▼]

The two-spotted spider mite, Tetranychus urticae Koch, is a worldwide pest which feeds on a large variety of plant families. Because its resistance to acaricides is spreading rapidly, the development of new biological control tactics for population management is crucial. Plant extracts, such as Santolina africana and Hertia cheirifolia (Asteraceae), may represent viable alternatives, since they are currently considered to be minimumrisk pesticides. Although these two plants are known for their biocidal properties, the extract concentration that provides the most efficient control has not yet been precisely determined. In this study, we conducted a series of laboratory experiments to determine the susceptibility of adult females to different concentrations of S. africana and H. cheirifolia extract. These plants were steam-distilled and sprayed using a Potter spray tower. Mortality and fecundity were measured upon treatment with extract concentrations ranging from 0.07 to 6.75 mg/L. Female mortality increased with concentration, with LD50 values of 2.35 mg/L for S. africana and 3.43 mg/L for H. cheirifolia, respectively Reduced fecundity was previously observed at concentrations of 0.07, 0.09 and 0.29 mg/L .The chemical composition of the two oils were characterised by GC-MS. The most abundant components of the S. africana oil were terpinen-4-ol (44%), α- terpineol (11.31%) and borneol (6.73%). Camphor (15.11%), terpinen-4-ol (14.2%), α- terpineol (9.3%) were characteristic of the Hertia oil. [less ▲]

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