Valorisation du colostrum bovin. Rapport Scientifique et Technique Final

Report (2006)

Mapping and polymorphism of bovine ghreline gene

Poster (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

Valorisation du colostrum bovin. Rapport Scientifique et technique. Année 3.

Report (2006)

Effet du colostrum bovin administré à des porcelets au sevrage sur la morphologie de la paroi intestinale et sur le système immunitaire

in 37èmes journées de la recherche porcine (2005, February)

Two groups (A and B) of 6 piglets, weaned at 21 days (D0), received ad libitum the same diet during three weeks. The piglets of group A were not submitted to a colostrum supplementation while those of the ... [more ▼]

Two groups (A and B) of 6 piglets, weaned at 21 days (D0), received ad libitum the same diet during three weeks. The piglets of group A were not submitted to a colostrum supplementation while those of the group B received daily and orally 5 g of bovine colostrum. Each week, piglets were weighed and blood was taken on each one of them. One piglet of each group was sacrified on D0 and the rest of the animals on D21. Mesenteric lymph nodes (ML), iléal Peyer patches (iPP), jejunum and spleen biopsies were taken on each piglet before death. The haematological values and the intestinal histo-morphological data between the two groups were not significantly different, but FACS analyses on the lymphocytes in the biopsies and the determination of stimulation index suggested a significative effect of colostrum on the lymphocytes populations, with, in the ML, an increase of the LB population (14 % vs 28 %, P < 0,05) and, in the iPP, a reduction of the LB's (54,99 % vs 8,44 %, P < 0,01) with an increase of the LT cells (16,87 % vs 72,00 %, P < 0,001). An increase of the lymphocyte stimulation index from piglets receiving colostrum was observed the second day of in vitro stimulation with colostrum. In brief, the answer of the immunity system to bovine colostrum seems to be restricted to the intestinal immunity system and is characterised by an activation, a multiplication and a modification of the lymphocytes ratios. [less ▲]