References of "HUYNEN, Pascale"
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See detailSéminaire : role et place du technologue dans un laboratoire
HUYNEN, Pascale ULg

Scientific conference (2016, December 15)

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See detailHow to use serology in Sexually Transmitted Infections
HUYNEN, Pascale ULg; LIBOIS, Agnes

Conference (2016, December 02)

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See detailLaboratory diagnosis of syphilis
HUYNEN, Pascale ULg

Conference (2016, September 26)

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See detailBorréliose de Lyme : aspects cliniques et prise en charge thérapeutique
HUYNEN, Pascale ULg

Scientific conference (2016, June 22)

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See detailToxoplasma gondii, cytomegalovirus, and Treponema pallidum infections in pregancy: what's new?
HUYNEN, Pascale ULg; LAZZAROTTO, Tiziana; PEYRON, François

in Wiener Klinisches Magazin (2016), 1(3), 1-8

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See detailL'automatisation des selles dans un laboratoire de Sérologie
HUYNEN, Pascale ULg

Conference (2016, April 21)

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See detailSyphilis during pregnancy
HUYNEN, Pascale ULg

Conference (2016, April 12)

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See detailA One-year Survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterisation
SACHELI, Rosalie ULg; ADJETEY BAHUN, Akolé ULg; DARFOUF, Rajae ULg et al

in Clinical Microbiology and Infection (2016), 22(3), 2859-28517

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the ... [more ▼]

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the emergence of this species in the country, the Belgian National Reference Center (NRC) for dermatophytes launched an epidemiological survey on the main anthropophilic dermatophytes causing TC in Belgium and including the genomic characterization of M. audouinii isolates. In total, 116 strains of M. audouinii were confirmed and characterized by the DiversiLab® system (bioMérieux). Six genotypic variants were identified, among which one major group included 90 isolates and the reference strain. Another variant group (11 strains) was exclusively confined to a geographical region in South Belgium. Analysis of epidemiological characteristics of the infected population showed that the main age category concerned 5-9 year-old children with a sex ratio (Male/Female) of 1.97. Data concerning the geographic origin of the family revealed a majority of Belgian nationality (44.7%), suggesting that the contamination has originated in Belgium. Other nationalities primarily originated from Africa. At this time, no clear correlation has been established between one particular strain and a specific country of origin. [less ▲]

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See detailGestion des épidémies à norovirus
HUYNEN, Pascale ULg; DUMASY, Axelle

Scientific conference (2016, January 19)

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See detailHuman norovirus infection in Latin America
da Silva Polo, Tatiane; Peiro, Juliana; Claudio Nogueira Mendes, Luiz et al

in Journal of Clinical Virology (2016), 78

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See detailHow efficient and automated can be Serology and Stool Testing?
HUYNEN, Pascale ULg

in Year (2015), 13(9), 2-4

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See detailEpidemiological aspects and genotypic characterization of T.violaceum strains collected during a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Dekkers, Charlotte; GRAIDE, Hélène ULg et al

in Mycoses (2015, October), 58(Supplement 4), 189

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC Liège) launched a one-year national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii and T. violaceum strains isolated from hair to the NRC with a form to fill in including epidemiological data. The fungal strains were identified by microscopy or ITS sequencing in case of doubtful identification. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological data were analyzed with the help of a biostatistician. Results Amongst the collected isolates, 23 strains were confirmed as T.violaceum (results concerning the 116 M. audouinii strains have already been reported). Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 0-4 year-old children (n=9, 39,1%) with a sex-ratio M/F of 1.875. Data concerning the geographic origin of the family were present in 82,6% of the cases and reveal that patients were mainly of Ethiopian origin (n=8, 57,9% of known cases). One patient was also from Burundi showing that T. violaceum strains probably circulate mainly in East Africa. The genotypic analysis led to the distinction of 2 variants of T. violaceum. The major group was composed of 17 strains which were mainly collected in the North of Belgium and included also the reference strain (18/23, 83,3%). The other group (6 strains) was close to the major group but the analysis of the spectral superposition showed some differences between these two groups, defining two distinct variants of T. violaceum in the Belgian population. This second variant was mainly recovered from South Belgium (5/6, 83,3%). No correlation could be made between the genotypic group and a particular ethnical origin as Ethiopian subjects were found in both groups. Conclusion The DiversiLab® system proved to be an efficient method to investigate the molecular epidemiology of dermatophytes infections as reported previously for M. audouinii. These results show that two distinct isolates co-exist in Belgium providing evidence of genetic heterogeneity and a possible spread of one genotypic variant in a restricted geographic area or the co-existence of two variants circulating in different African communities. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as age or ethnical origin. [less ▲]

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See detailQuality and Innovation, key factors for laboratory evolution
HUYNEN, Pascale ULg

Conference (2015, June 23)

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See detailQuality and Innovation, key factors for laboratory evolution
HUYNEN, Pascale ULg

in Clinical Chemistry & Laboratory Medicine (2015, June 23)

Today the laboratory has to face many challenges: constant increase of number of tests to be run, private labs that competes in reaching a lower TAT, disease outbreak that arises without the possibility ... [more ▼]

Today the laboratory has to face many challenges: constant increase of number of tests to be run, private labs that competes in reaching a lower TAT, disease outbreak that arises without the possibility of human control, like the recent mumps outbreak, the need to provide fast results in case of emergency or for transplants, the request to keep high level of traceability of all results, the accreditation of the lab are just some examples. With the same number of operators, year after year, new clinical needs have to be satisfied in a timely manner, with efficiency and without compromise in quality. The solution for us has been, across several year, to look for innovation. Moving from Elisa to chemiluminescence and therefore from open systems to close and state of the art systems, it has allowed us to face with success all those challenges. The availability of more and more infectious disease markers on fully automated analyzers, with good level of performance, have let us to cope with all the changes that have happened across more than a decade. Indeed innovation and quality are fundamental to support properly the laboratory evolution that occurred since today and it is still occurring. Innovation in our laboratory it is also represented by the introduction of automatized tests not only on serum and plasma specimens, but also on CSF (for Lyme disease diagnosis) and on stool matrix. In 2013 in fact we have introduced, among the assays already tested in our laboratory, two assays performed on this matrix, the C. difficile Toxin A&B and GDH, due to the possibility offered by the LIAISON® systems to run all of them on the same serology platform, without crosscontamination. New markers will be available in the near future, and our laboratory will be always able to meet the next clinical needs. [less ▲]

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See detailNorovirus: ce que les praticiens de soins devraient savoir.
HUYNEN, Pascale ULg

Conference (2015, June 01)

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See detailHow efficiency and automated can be serology and stool testing?
HUYNEN, Pascale ULg

in Clinical Chemistry & Laboratory Medicine (2015), 53(S1), 161

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Dekkers, Charlotte; DARFOUF, Rajae ULg et al

Poster (2015, May)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________ [less ▲]

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Géron, Bénédicte; Dekkers, Charlotte et al

Poster (2015, April 28)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 97 strains have been currently confirmed as M. audouinii. Preliminary analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (84%) with a sex-ratio M/F of 1.95. Data concerning the geographic origin of the family have been obtained in only 45.8% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (77%) suggesting bias in the data collection. The geographic origin of the remaining group (23%) includes several African countries. The genotypic analysis led to the distinction of 3 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 85 strains, well distributed in different Belgium locations. The last group contains only one strain but this strain was significantly different from the two other variants. Conclusion The automated typing DiversiLab® system proved to be an easy and efficient method to investigate the molecular epidemiology of dermatophytes infections. These preliminary results show that, through Belgium, several groups of isolates co-exist for M. audouinii providing evidence of genetic heterogeneity. At this time all epidemiological informations have not yet been assessed while 35 strains of M. audouinii remain to be analysed genotypically to give definitive conclusions. [less ▲]

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See detailDiagnostic microbiologique des infections respiratoires virales en Pédiatrie
HUYNEN, Pascale ULg

Conference (2015, March 05)

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