References of "Gustin, Pascal"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailWhat does multiplication of tidemarks mean in the ovine femora-tibial joint ?
Pirson, R; Matagne, A; Nisolle, JF et al

in Osteoporosis International (2014), 25(S2), 172

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailBiochemical composition of cartilage with naturally occurring defects in the ovine femora-tibial joint
Matagne, A; Clegg, P; Tew, S et al

in Osteoporosis International (2014), 25(S2), 173

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailPrevalence of naturally occurng cartilage defects in the ovine knee
Vandeweerd, Jean-Michel; Hontoir, Fanny; Kirschvink, Nathalie et al

in Osteoarthritis and cartilage (2013), 21

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailIs evidence-based medicine so evident in veterinary research and practice? History, obstacles and perspectives.
Vandeweerd, Jean-Michel ULg; Kirschvink, Nathalie; Clegg, Peter et al

in Veterinary Journal (2012), 191(1), 28-34

Evidence-based medicine (EBM) refers to the conscientious, explicit and judicious use of current best evidence from research for the care of an individual patient. The concept of EBM was first described ... [more ▼]

Evidence-based medicine (EBM) refers to the conscientious, explicit and judicious use of current best evidence from research for the care of an individual patient. The concept of EBM was first described in human medicine in the early 1990s and was introduced to veterinary medicine 10 years later. However, it is not clear that the EBM approach promulgated in human medicine can be applied to the same extent to veterinary medicine. EBM has the potential to help veterinarians to make more informed decisions, but obstacles to the implementation of EBM include a lack of high quality patient-centred research, the need for basic understanding of clinical epidemiology by veterinarians, the absence of adequate searching techniques and accessibility to scientific data bases and the inadequacy of EBM tools that can be applied to the busy daily practise of veterinarians. This review describes the development of EBM in the veterinary profession, identifies its advantages and disadvantages and discusses whether and how veterinary surgeons should further adopt the EBM approach of human medicine. [less ▲]

Detailed reference viewed: 50 (11 ULg)
Full Text
Peer Reviewed
See detailCan beta2-adrenoceptor agonists, anticholinergic drugs, and theophylline contribute to the control of pulmonary inflammation and emphysema in COPD?
Zhang, Wen-Hui; Zhang, Yong; Cui, Yong-Yao et al

in Fundamental & Clinical Pharmacology (2012), 26(1), 118-134

Chronic obstructive pulmonary disease (COPD) has become a global epidemic disease with an increased morbidity and mortality in the world. Inflammatory process progresses and contributes to irreversible ... [more ▼]

Chronic obstructive pulmonary disease (COPD) has become a global epidemic disease with an increased morbidity and mortality in the world. Inflammatory process progresses and contributes to irreversible airflow limitation. However, there is no available therapy to better control the inflammatory progression and therefore to reduce the exacerbations and mortality. Thus, the development of efficient anti-inflammatory therapies is a priority for patients with COPD. beta(2) -Adrenoceptor agonists and anticholinergic agents are widely used as first line drugs in management of COPD because of their efficient bronchodilator properties. At present, many studies in vitro and some data obtained in laboratory animals reveal the potential anti-inflammatory effects of these bronchodilators but their protective role against chronic inflammation and the development of emphysema in patients with COPD remains to be investigated. The anti-inflammatory effects of theophylline at low doses have also been identified. Beneficial interactions between glucocorticoids and bronchodilators have been reported, and signaling pathways explaining these synergistic effects begin to be understood, especially for theophylline. Recent data demonstrating interactions between anticholinergics with beta(2) -adrenoceptor agonists aiming to better control the pulmonary inflammation and the development of emphysema in animal models of COPD justify the priority to investigate the interactive effects of a tritherapy associating corticoids with the two main categories of bronchodilators. [less ▲]

Detailed reference viewed: 24 (4 ULg)
Full Text
Peer Reviewed
See detailSystematic review of efficacy of nutraceuticals to alleviate clinical signs of osteoarthritis.
Vandeweerd, Jean-Michel ULg; Coisnon, C.; Clegg, P. et al

in Journal of Veterinary Internal Medicine (2012), 26(3), 448-56

BACKGROUND: Various treatments of osteoarthritis (OA) have been described, including use of nutraceuticals. OBJECTIVES: To review systematically the literature about the effects of nutraceuticals on ... [more ▼]

BACKGROUND: Various treatments of osteoarthritis (OA) have been described, including use of nutraceuticals. OBJECTIVES: To review systematically the literature about the effects of nutraceuticals on clinical signs of pain or abnormal locomotion in horses, dogs, and cats, and to discuss methodological aspects of trials and systematic reviews. METHODS: A systematic search of controlled trials evaluating the impact of nutraceuticals on OA in horses, dogs, and cats was performed, using Medline, CAB Abstracts, and Google Scholar. Scientific evidence was evaluated by means of criteria proposed by the Food and Drug Administration (FDA), and a scoring system adapted from both the CONsolidated Standards of Reporting Trials (CONSORT) statement and recommendations for assessing trials by the Center of Evidence Based Medicine of Oxford. RESULTS: Twenty-two papers were selected and reviewed, with 5 studies performed in horses, 16 in dogs, and 1 in cats. The strength of evidence was low for all nutraceuticals except for omega-3 fatty acid in dogs. There were limited numbers of rigorous randomized controlled trials and of participants in clinical trials. CONCLUSIONS AND CLINICAL IMPORTANCE: The evidence of efficacy of nutraceuticals is poor, with the exception of diets supplemented with omega-3 fatty acids in dogs. Greater access to systematic reviews must be part of the objectives of the veterinary science in the future. Their reporting would be improved by internationally agreed-upon criteria for standards and guidelines. [less ▲]

Detailed reference viewed: 28 (2 ULg)
Full Text
Peer Reviewed
See detailPrevalence of naturally occuring cartilage defects in the ovine stifle
Pierson, Audrey ULg; Muylkens, Benoît ULg; Hontoir, Fanny et al

Poster (2012)

Detailed reference viewed: 27 (9 ULg)
Full Text
Peer Reviewed
See detailUnderstanding veterinary practitioners' decision-making process: implications for veterinary medical education
Vandeweerd, Jean-Michel ULg; Vandeweerd, Solene; Gustin, Catherine et al

in Journal of Veterinary Medical Education (2012), 39(2), 142-151

Understanding how veterinary practitioners make clinical decisions, and how they use scientific information to inform their decisions, is important to optimize animal care, client satisfaction, and ... [more ▼]

Understanding how veterinary practitioners make clinical decisions, and how they use scientific information to inform their decisions, is important to optimize animal care, client satisfaction, and veterinary education. We aimed to develop an understanding of private practitioners' process of decision making. On the basis of a grounded-theory qualitative approach, we conducted a telephone survey and semi-structured face-to-face interviews. We identified a decision-making framework consisting of two possible processes to make decisions, five steps in the management of a clinical case, and three influencing factors. To inform their decision, veterinary surgeons rarely take the evidence-based medicine (EBM) approach. They consult first-opinion colleagues, specialists, laboratories, and the Internet rather than scientific databases and peer-reviewed literature, mainly because of limited time. Most interviewees suggested the development of educational interventions to better develop decision-making skills in veterinary schools. Adequate information and EBM tools are needed to optimize the time spent in query and assessment of scientific information, and practitioners need to be trained in their use. [less ▲]

Detailed reference viewed: 34 (5 ULg)
Full Text
Peer Reviewed
See detailSalmeterol or doxycycline do not inhibit acute bronchospasm and airway inflammation in cats with experimentally-induced asthma
Leemans, J.; Kirschvink, N.; Bernaerts, Frederique ULg et al

in Veterinary Journal (2011)

The objective of this study was to determine if inhaled salmeterol, a long-acting β2-adrenergic agonist, and oral doxycycline, a tetracycline antibiotic displaying matrix metalloproteinase (MMP ... [more ▼]

The objective of this study was to determine if inhaled salmeterol, a long-acting β2-adrenergic agonist, and oral doxycycline, a tetracycline antibiotic displaying matrix metalloproteinase (MMP) inhibitory activity, reduce airway inflammation and obstruction in cats with experimentally-induced asthma. Eight Ascaris suum (AS)-sensitised cats were enrolled in a prospective study in which they underwent four AS-challenges at 1 month intervals. The challenged animals were given no treatment or were treated on 4 consecutive days with either: (1) oral prednisolone (1 mg/kg twice daily), (2) inhaled salmeterol (50 μg twice daily), or (3) oral doxycycline (5 mg/kg twice daily), according to a randomised cross-over design. Inhibition of allergen-induced early (EAR) and late (LAR) asthmatic reactions were assessed by barometric whole-body plethysmography. Cytology and measurement of MMP-2 and -9 activities were carried out on bronchoalveolar lavage fluid (BALF). Although none of the treatments prevented the EAR, prednisolone treatment inhibited the LAR. Relative to untreated cats, the eosinophil percentage and MMP-2 activity in BALF were significantly reduced following prednisolone treatment (P < 0.05). Short-term therapy with either salmeterol or doxycycline had no effect on the EAR or LAR or on airway inflammation. Given the chronic nature of this disease in cats, long-term therapy may be required to produce more favourable functional and clinical outcomes. [less ▲]

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailEffect of short-term oral and inhaled corticosteroids on airway inflammation and responsiveness in a feline acute asthma model
Leemans, Jérôme; Kirschvink, Nathalie; Clercx, Cécile ULg et al

in Veterinary Journal (2011)

The objective of this study was to investigate whether high-dose inhaled fluticasone propionate (FP), alone or in combination with salmeterol (SAL), is as effective as oral prednisolone in reducing airway ... [more ▼]

The objective of this study was to investigate whether high-dose inhaled fluticasone propionate (FP), alone or in combination with salmeterol (SAL), is as effective as oral prednisolone in reducing airway inflammation and obstruction in cats with experimentally-induced acute asthma. Six cats sensitised to Ascaris suum (AS) were enrolled in a prospective controlled therapeutic trial and underwent four aerosol challenges, at 1-month intervals with AS allergen. The allergen - stimulated animals received four consecutive days treatment with either oral prednisolone at 1mg/kg twice daily, 500μg of FP inhaled twice daily, or a combination of FP/SAL at 500μg/50μg inhaled twice daily, respectively, according to a randomised cross-over design. Treatment-related changes in lung function, airway responsiveness (AR) and bronchoalveolar lavage fluid (BALF) cytology were assessed. Barometric whole-body plethysmography (BWBP) was used for the assessment of respiratory variables and AR. No significant differences in respiratory rate or Penh (an estimate of airflow limitation measured by BWBP) were detected among treatment groups. Allergen-induced airway hyper-responsiveness was significantly inhibited by all three steroid treatments (P<0.05). The mean BALF eosinophil percentage (±SEM) was lower after oral and inhaled corticosteroid treatment and these changes were significant for groups receiving prednisolone and the FP/SAL combination. Findings suggest high-dose FP, particularly in combination with SAL, is effective in ameliorating airway inflammation and hyper-responsiveness in this model of acute feline asthma, and highlight the potential use of these drugs in cats experiencing acute exacerbations of the naturally occurring disease. [less ▲]

Detailed reference viewed: 34 (14 ULg)
Full Text
Peer Reviewed
See detailEffects of formoterol and ipratropium bromide on repeated cadmium inhalation -induced pulmonary inflammation and emphysema in rats
Zhang, Whenhui; Fievez, Laurence ULg; Zhang, F. et al

in European Journal of Pharmacology (2010), 25(647), 178-187

Detailed reference viewed: 54 (9 ULg)
Full Text
Peer Reviewed
See detailProphylactic effects of omega-3 polyunsaturated fatty acids and luteolin on airway hyperresponsiveness and inflammation in cats with experimentally-induced asthma.
Leemans, Jérôme ULg; Cambier, Carole ULg; Chandler, T. et al

in Veterinary Journal (2010)

The aim of this study was to assess the preventive effects of omega-3 polyunsaturated fatty acids (omega3 PUFA) and luteolin supplementation on allergen-induced inflammation in eight Ascaris suum (AS ... [more ▼]

The aim of this study was to assess the preventive effects of omega-3 polyunsaturated fatty acids (omega3 PUFA) and luteolin supplementation on allergen-induced inflammation in eight Ascaris suum (AS)-sensitised cats. Airway responsiveness (AR) tests were performed and venous blood and bronchoalveolar lavage fluid (BALF) collected before and following a single (AS-stimulated) allergen exposure, as well as at the end of a 4-week treatment period, which was followed by a second AS-challenge. The omega6/omega3 fatty acid ratio in erythrocyte membranes, BALF cytology, AR to carbachol, and BALF lipoxin A(4) (LXA(4)), an endogenous inhibitor of inflammation, were assessed at each time point. Compared to respective unstimulated values, AS-challenged cats exhibited a significant rise in BALF eosinophil percentage and there was a trend to increased BALF total cell counts, increased AR and reduced BALF LXA(4) concentrations. The significant decrease in the blood omega6/omega3 ratio seen after supplementation demonstrated that omega3 PUFA were efficiently absorbed. No changes in BALF cytology were found between untreated and treated AS-stimulated cats, but BALF LXA(4) levels were significantly elevated and AR significantly decreased following supplement intake. The study suggests that omega3-luteolin supplementation may have some beneficial effects on AR through a LXA(4)-dependent pathway in cats with experimentally-induced asthma. [less ▲]

Detailed reference viewed: 75 (13 ULg)
Full Text
Peer Reviewed
See detailFunctional response to inhaled salbutamol and/or ipratropium bromide in Ascaris suum-sensitised cats with allergen-induced bronchospasms
Leemans, Jérôme; Kirschvink, N.; Clercx, Cécile ULg et al

in Veterinary Journal (2010)

Knowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. A randomised controlled four-way ... [more ▼]

Knowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. A randomised controlled four-way crossover study was therefore designed to compare the effects of salbutamol (SAL, 100 μg), ipratropium bromide (IB, 20 μg) and a combination of both (SAL/IB, 100 μg/20 μg), delivered through a pressurised metered-dose inhaler (pMDI) connected to a spacing chamber, on allergen-induced bronchospasms in five Ascaris suum (AS)-sensitised cats. Four AS bronchial provocation challenges were carried out at 1 week intervals, followed by one of four treatment protocols: SAL, IB, SAL/IB or control (untreated). Enhanced pause (Penh), an estimator of airflow limitation measured by barometric whole-body plethysmography, was repeatedly assessed within 120 min following the administration of each treatment protocol. Responses to inhaled medications were evaluated by calculating the area under the time-response curves (AUC) from 0 to 60 or 120 min after drug administration (AUC(0-60), AUC(0-120)), as well as the times required for half-recovery (T(50%)) or for returning to nearly basal conditions (T(20%)). No significant differences were found among the four study groups, with reference to the mean AUC(0-60), T(20%) and T(50%) values of Penh (P>0.05). Mean AUC(0-120) values of Penh were similar between the bronchodilators tested, but were significantly lower than those in the untreated group. It was concluded that inhalation of SAL, IB and SAL/IB via pMDI failed to improve most Penh-derived parameters, which suggested that these bronchodilators were of limited efficacy in reversing allergen-induced bronchospasm in cats. However, further studies using a larger number of animals are warranted to investigate if different drugs or delivery devices or higher dosages may be more effective [less ▲]

Detailed reference viewed: 35 (2 ULg)
Full Text
Peer Reviewed
See detailCombined pharmacokinetic and urodynamic study of the effects of oral administration of phenylpropanolamine in female Beagle dogs
Noël, Stéphanie; Cambier, Carole; Baert, Kris et al

in Veterinary Journal (2010)

This study investigated the differences in pharmacokinetic, urodynamic and haemodynamic parameters in female Beagle dogs in which blood sampling and urethral pressure profiles were performed over 24 h ... [more ▼]

This study investigated the differences in pharmacokinetic, urodynamic and haemodynamic parameters in female Beagle dogs in which blood sampling and urethral pressure profiles were performed over 24 h periods, after single or three times daily (T0-T6h-T12h) administration of phenylpropanolamine (PPA). Maximal concentration (Cmax) was reached 2 h after PPA administration (Tmax). The half-life lasted 4 h and, because of bioaccumulation, three times daily administration induced an increase in Cmax. Compared to the control group, a significant increase in urethral resistance was observed at Tmax after 1 week of once daily administrations. After one week of administering PPA every 6 h during the daytime, but not during the night, and despite higher plasma concentrations, the urethral resistance did not increase at any time, compared to the control group. The increase in mean arterial pressure values was compensated by a decreased heart rate. The clinical efficacy of the temporary increase in urethral resistance following single daily administration of PPA in dogs suffering from urethral sphincter mechanism incompetence (USMI) needs to be further investigated in a randomised clinical trial. [less ▲]

Detailed reference viewed: 101 (21 ULg)
Full Text
Peer Reviewed
See detailEffects of ipratropium bromide on repeated cadmium inhalation-induced lune inflammation and airspace enlargement in rats
Zhang, W. H.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Fundamental & Clinical Pharmacology (2010), 10

Detailed reference viewed: 28 (3 ULg)
Full Text
Peer Reviewed
See detailAnti-inflammatory effects of formoterol and ipratropium bromide against acute cadmium-induced pulmonary inflammation in rats.
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in European Journal of Pharmacology (2010), 628(1-3), 171-178

In this study, the anti-inflammatory properties of formoterol and ipratropium bromide, alone or in combination, were investigated in a rat model of acute pulmonary inflammation induced by cadmium ... [more ▼]

In this study, the anti-inflammatory properties of formoterol and ipratropium bromide, alone or in combination, were investigated in a rat model of acute pulmonary inflammation induced by cadmium inhalation. Airway resistance and inflammatory responses, including matrix metalloproteinease-2 (MMP-2) and matrix metalloproteinease-9 (MMP-9) activities, were evaluated. Compared to values obtained in rats exposed to cadmium, pretreatment by bronchodilators administered alone significantly prevented the cadmium-induced increase of airway resistance. Formoterol elicited a significant decrease in total cell number, neutrophil and macrophage counts in bronchoalveolar lavage fluid, whereas ipratropium bromide reduced neutrophil numbers. The two compounds administered alone significantly attenuated the lung lesions associated with parenchyma inflammatory cell influx and congestion observed in the cadmium group. The increased MMP-9 activity was significantly attenuated. Although only formoterol induced a decrease protein concentration in bronchoalveolar lavage fluid, both compounds inhibited the pulmonary edema by reducing wet-to-dry weight ratio which returned to values similar to those recorded in the sham group. All the effects of formoterol on the cadmium-induced inflammatory responses were reversed by propranolol. Similar anti-inflammatory effects were obtained in rats pretreated with ilomastat which showed a significant reduction on inflammatory cell infiltration and MMP-9 activity in bronchoalveolar lavage fluid. Neither synergistic nor additive effects were obtained when the two bronchodilators were administered in combination. In conclusion, formoterol and ipratropium bromide partially protect the lungs against the inflammation by reducing neutrophilic infiltration. This protective effect is associated with reduced MMP-9 activity known to play an important pro-inflammatory role in acute inflammatory process. [less ▲]

Detailed reference viewed: 82 (10 ULg)
Full Text
Peer Reviewed
See detailPenetration of enrofloxacin into the nasal secretions and relationship between nasal secretions and plasma enrofloxacin concentrations after intramuscular administration in healthy pigs
Bimazubute, M.; Cambier, Carole ULg; Baert, K. et al

in Journal of Veterinary Pharmacology & Therapeutics (2010), 33(2), 183-188

The pharmacokinetic behaviour of enrofloxacin (ENRO) in plasma and nasal secretions of healthy pigs was investigated, after a single-dose intramuscular administration of 2.5 mg/kg body weight of the drug ... [more ▼]

The pharmacokinetic behaviour of enrofloxacin (ENRO) in plasma and nasal secretions of healthy pigs was investigated, after a single-dose intramuscular administration of 2.5 mg/kg body weight of the drug. Blood samples and nasal secretions were collected at predetermined times after drug administration. Concentrations of ENRO and its active metabolite ciprofloxacin (CIPRO) were determined in plasma and nasal secretions by high-performance liquid chromatography (HPLC). CIPRO was not detected probably because we investigated young weaned pigs. The data collected in 12 pigs for ENRO were subjected to noncompartmental analysis. In plasma, the maximum concentration of drug (C-max), the time at which this maximum concentration of drug (T-max) was reached, the elimination half-life (t(beta)(1/2)) and the area under the concentration vs. time curve (AUC) were, respectively, 694.7 ng/mL, 1.0 h, 9.3 h and 8903.2 ng h/mL. In nasal secretions, Cmax, Tmax, t(beta)(1/2) and AUC were, respectively, 871.4 ng/mL, 2.0 h, 12.5 h and 11 198.5 ng.h/mL. In a second experiment conducted in 10 piglets, the relationship between concentrations of ENRO measured in the plasma and the nasal secretions has been determined following single-dose intramuscular administration of 2.5, 10 or 20 mg/kg body weight of the drug. It has been demonstrated that, among several variables, i.e., (1) the dose administered, (2) the time between intramuscular injection and blood sampling, (3) the age, (4) the sex, (5) the animal body weight and (6) the plasma concentration of the drug, only the latter influenced significantly the ENRO concentration in nasal secretions. Practically, using a generalized linear mixed model, ENRO concentrations in the nasal secretions (mu g/mL) can be predicted taking into account the ENRO concentrations in plasma (mu g/mL), according to the following equation: ENROnasal secretion 1.94 ENROplasma - 0.24. [less ▲]

Detailed reference viewed: 54 (11 ULg)
Full Text
Peer Reviewed
See detailA crucial role forlung interstitial macrophages in preventing airway allergy
Bedoret, Denis; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Short book of the Annual Congress of the European Respiratory Society (ERS), Vienne (2009)

Detailed reference viewed: 36 (14 ULg)
Peer Reviewed
See detailLung interstitial macrophages prevent the development of respiratory allergy
Bedoret, D.; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Proceedings of The Keystone Symposia: Allergy and Asthma. Keystone, Colorado, USA (2009)

Detailed reference viewed: 58 (20 ULg)