The practical challenges of the Biosafety officer : interacting with the user

in The Biosafety Professional: roles and interactions in a complex network (2008)

Implementing BSL II & BSL III activities in an existing facility : the case of the Animal facility of the CHU at University of Liège, Belgium "

(2006, May 10)

The antitumoral effect of endostatin and angiostatin is associated with a down-regulation of vascular endothelial growth factor expression in tumor cells

in FASEB Journal (2002), 16

Endostatin and angiostatin are known as tumor-derived angiogenesis inhibitors, but their mechanisms of action are not yet completely defined. We report here that endostatin and angiostatin, delivered by ... [more ▼]

Endostatin and angiostatin are known as tumor-derived angiogenesis inhibitors, but their mechanisms of action are not yet completely defined. We report here that endostatin and angiostatin, delivered by adenoviral vectors, reduced in vitro the neovessel formation in the mouse aortic ring assay by 85 and 40%, respectively. We also demonstrated in vivo that both endostatin and angiostatin inhibited local invasion and tumor vascularization of transplanted murine malignant keratinocytes, and reduced by 50 and 90% the development of highly vascularized murine mammary tumors. This inhibition of tumor growth was associated with a reduction of tumor vascularization. Expression analysis of vascular endothelial growth factor (VEGF) carried out in the mouse aortic ring model revealed a 3- to 10-fold down-regulation of VEGF mRNA expression in endostatin-treated rings. A similar down-regulation of VEGF expression at both mRNA and protein levels was also observed in the two in vivo cancer models after treatment with each angiogenesis inhibitor. This suggests that endostatin and angiostatin effects may be mediated, at least in part, by their ability to down-regulate VEGF expression within the tumor. This work provides evidence that endostatin and angiostatin act on tumor cells themselves. [less ▲]

The role of cellular and prodrug-associated factors in the bystander effect induced by the Varicella zoster and the Herpes simplex virus thymidine kinases in suicide gene therapy

in Cancer Gene Therapy (2000), 7

Potential of Varicella zoster virus thymidine kinase as a suicide gene in breast cancer cells

in Gene Therapy (1997), 4