References of "Grandfils, Christian"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailStudy of the specific interaction between Fluorescent PDMAEMA and human Platelets
Flebus, Luca ULg; Lombart, François ULg; Martinez, Lucia et al

Conference (2014, July 12)

Hemoreactivity of the poly (2-(dimethylamino) ethylmethacrylate) polymer (PDMAEMA) under a free form (thus not as a “PolyElectrolyte Complex”) was assessed. In order to facilitate the in vitro monitoring ... [more ▼]

Hemoreactivity of the poly (2-(dimethylamino) ethylmethacrylate) polymer (PDMAEMA) under a free form (thus not as a “PolyElectrolyte Complex”) was assessed. In order to facilitate the in vitro monitoring of this polycation and especially to follow its reactivity blood we have labeled it with fluorescein adopting a new chemical route of synthesis. Unexpectedly, using flow cytometry, this study showed a higher affinity of PDMAEMA for platelets than for red blood cells. [less ▲]

Detailed reference viewed: 14 (3 ULg)
Full Text
See detailThe potentiality of PDMAEMA-based polycation for cardiovascular applications
Flebus, Luca ULg; Lombart, François ULg; Martinez, Lucia et al

Conference (2014, June 18)

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailBiodegradable polyester-based microcarriers with modified surface tailored for tissue engineering
Privalova, A.; Markvicheva, E.; Sevrin, Chantal ULg et al

in Journal of Biomedical Materials Research: Part A (2014)

Detailed reference viewed: 16 (4 ULg)
Full Text
See detailCompetitive interaction of a synthetic polycation (PDMAEMA) to human platelets and erythrocytes
Flebus, Luca ULg; Lombart, François ULg; martinez, Lucia et al

Poster (2013, October)

Detailed reference viewed: 22 (5 ULg)
Full Text
Peer Reviewed
See detailProduction of anionic extracellular polysaccharide by Enterobacter A47 using cheese whey as feedstock
Antunes, S; Alves, V.D.; Grandfils, Christian ULg et al

Conference (2013, September)

Detailed reference viewed: 11 (0 ULg)
Full Text
See detailDevelopment of new biomimetic biopolymers
Grandfils, Christian ULg

Scientific conference (2013, June 27)

Detailed reference viewed: 11 (0 ULg)
Full Text
See detailMosquito net for abdominal wall repair: a comparison of their chemical, physical, morphological and mechanical properties to commercial meshes
Sevrin, Chantal ULg; Nsadi Fwene, Berthier; Arung, Willy et al

in Biomedica proceedings (2013, June 23)

Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor ... [more ▼]

Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor developing countries a traditional sutured repair is still commonplace although with significantly inferior results. Sterilized mosquito nets have been recently roposed by several authors to replace medical meshes in low-income countries . However in the perspective of their clinical use a better understanding of this mosquito net is obviously requested both in terms of material properties and of in vivo biocompatibility. [less ▲]

Detailed reference viewed: 32 (7 ULg)
Full Text
Peer Reviewed
See detailHemoreactivity of polymeric carriers designed for drug delivery systems
Grandfils, Christian ULg; Sevrin, Chantal ULg

in Proceeding Biomedica 2013 (2013, June 19)

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailMicroscopic techniques to study the interaction of new polymers within live cells
Smisdom, N.; Sanen, K.; Van Den Akker, N. et al

Poster (2013, June 19)

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailOptimization of biodegradable microparticles for tissue engineering
Drozdova, M.; Demina, T.; Sevrin, Chantal ULg et al

Poster (2013, June 19)

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailBiodegradable microspheres based on amphiphilic graft-copolymers of chitosan and polyesters
Demina, T; Drozdova, M.; Sevrin, Chantal ULg et al

Poster (2013, June 19)

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailIn vitro study of fibroblasts (L929) and osteoblasts (MG-63) within networks differing in fiber density.
Lombart, François ULg; Alexandre, Diane ULg; de Bien, Charlotte ULg et al

Poster (2013, June 18)

Animal cells are typically cultivated in vitro on 2D surfaces therefore in conditions totally differing from their 3D in vivo environments. It is therefore really attractive to generate better in vitro ... [more ▼]

Animal cells are typically cultivated in vitro on 2D surfaces therefore in conditions totally differing from their 3D in vivo environments. It is therefore really attractive to generate better in vitro animal cell models where animal cells can adhere and proliferate within a 3D networks. When facing to a third dimension, the design of the scaffolds tailored to support the organization and communication of cells should favor at least cell adhesion, proliferation and differentiation, while promoting nutrient, gas (O2 / CO2) and waste product diffusion. In order to construct this in vitro model, we have compared the cell reactivity of two model cell lines fibroblaste L 929 and osteoblaste MG-63 within three 3D networks differing in fiber density. This parameter has been altered in order to increase progressively the total surface exposed to the cells, whilst increasing correspondingly the mean pore size and total porosity of the network, whilst keeping the same architecture and surface chemistry of the fibers within the scaffold. [less ▲]

Detailed reference viewed: 31 (13 ULg)
Full Text
Peer Reviewed
See detailStudy of the specific interaction between fluorescent PDMAEMA and Platelets compared to Erythrocytes
Flebus, Luca ULg; Lombart, François ULg; Martinez, Lucia et al

Poster (2013, June 18)

Nowadays synthetic polymers provide more and more functionalities in the biomedical world, in particular as part of medical devices and drug delivery systems. Looking for new applications in the ... [more ▼]

Nowadays synthetic polymers provide more and more functionalities in the biomedical world, in particular as part of medical devices and drug delivery systems. Looking for new applications in the cardiovascular field, our attention has been focused on the well-known poly(2-(dimethylamino)ethyl methacrylate polymer (PDMAEMA). This synthetic polycation has particularly been studied as a potential DNA carrier to promote cell transfection. However and surprisingly enough relative few studies have been published in order to better understand its hemoreactivity under a free form (thus not based on “PolyElectrolyte Complex”), but also its biodistribution and clearance kinetics. In order to facilitate the in vitro and in vivo monitoring of this polycation and especially to follow its reactivity with whole blood we have labeled it with fluorescein adopting a new chemical route of synthesis. After its thorough purification and full-characterization (NMR, SEC, fluorescent spectroscopy), we followed its interaction with erythrocytes and platelets using flow cytometry. Dose-response curves were established in whole blood and within incubation times ranging between 5 min to 3 h. Considering the largest area and more negative Zeta potential developed by red blood cells compared to platelets, we initially hypothesized that RBC should interact more quickly and efficiently with the polycation. Unexpectedly, platelets presented a higher affinity for the polycation with a saturation binding curve whilst a linear profile was observed for the erythrocyte adsorption curve. Aggregometry analyses also revealed this phenomenon, although displaying a weaker effect in whole blood compared to washed platelets. Ongoing research seeks to understand the molecular mechanism of interaction of this polymer with platelets. [less ▲]

Detailed reference viewed: 22 (4 ULg)