References of "Grandfils, Christian"
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See detailHighly Luminescent Aqueous Ag2S Quantum Dots as New Generation Quantum Dots
Yağcı Acar, Havva Funda; Hocaoğlu, I.; Demir, F. et al

in Proceediing of the meeting (2015, March 09)

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See detailProduction of extracellular polysaccharide by Enterobacter A47 using cheese whey as the sole carbon source
Antunes, S.; Alves, V.D.; Grandfils, Christian ULg et al

in Proceeding of the meeting (2015, January 19)

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See detailCharacterization of the chitin-glucan complex extracted from Komagataella pastoris cell wall
Farinha, I.; Duarte, P.; Pimentel et al

in Proceeding of the meeting (2015, January 19)

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See detailLow molecular weight poly (2-dimethylamino ethylmethacrylate) polymers with controlled positioned fluorescent labeling: Synthesis, characterization and in vitro interaction with human endothelial cells.
Flebus, Luca ULg; Lombart, François ULg; Sevrin, Chantal et al

in International journal of pharmaceutics (2015), 478(1), 278-287

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical ... [more ▼]

Poly (2-dimethylamino ethylmethacrylate) (PDMAEMA) is an attractive non-degradable polymer studied as nonviral vector for gene delivery but it can be also adopted for delivery of other biopharmaceutical drugs. As a parenteral carrier, the PDMAEMA free form (FF) might interact with tissues and cells. Few data are available on its selective internalization and efflux from cells, while the majority of studies published have followed the distribution of DNA complexed with PDMAEMA. In order to address polycation safety, the first aim was to synthesize by atom transfer radical polymerisation (ATRP) fluorescent labeled PDMAEMA of low molecular weight (Mw) (below 15kDa), controlling the position and density of fluorescein. The second goal was to analyze the possible difference in uptake and subcellular distribution of this labeled FF polycation between human umbilical vein endothelial cells (HUVEC) and hCMEC/D3 cells. These two cell lines have been chosen in order to detect selectivity towards the blood-brain barrier (BBB). In both cases, polycation was detected along the plasma membrane followed by progressive migration to the peri-nuclear region, where it overlapped with lysosomal structures. The analysis by fluorescence-activated cell sorting (FACS) of the PDMAEMA uptake by hCMEC/D3 cells showed a significant (p<0.05) inhibition (40%) in presence of 2-dexoxy-d-glucose inhibitor, a result supporting an energy-dependence mechanism(s). Cytotoxicity study showed that low Mw PDMAEMA (10kDa) lead to a minor cytotoxicity compared to the higher ones. As main conclusion this study highlights the similitude in cell trafficking of FF PDMAEMA and data previously reported for PDMAEMA/DNA complexes. [less ▲]

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See detailIn vitro hemocompatibility assessment of Near-IR-Emitting Ag2S Quantum Dots (QD)
Grandfils, Christian ULg; Hocaoglu, I.; Demir, F. et al

in Proceeding of the Nanobiotechnology International Workshop (2014, November 26)

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See detailStudy of the specific interaction between Fluorescent PDMAEMA and human Platelets
Flebus, Luca ULg; Lombart, François ULg; Martinez, Lucia et al

Conference (2014, July 12)

Hemoreactivity of the poly (2-(dimethylamino) ethylmethacrylate) polymer (PDMAEMA) under a free form (thus not as a “PolyElectrolyte Complex”) was assessed. In order to facilitate the in vitro monitoring ... [more ▼]

Hemoreactivity of the poly (2-(dimethylamino) ethylmethacrylate) polymer (PDMAEMA) under a free form (thus not as a “PolyElectrolyte Complex”) was assessed. In order to facilitate the in vitro monitoring of this polycation and especially to follow its reactivity blood we have labeled it with fluorescein adopting a new chemical route of synthesis. Unexpectedly, using flow cytometry, this study showed a higher affinity of PDMAEMA for platelets than for red blood cells. [less ▲]

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See detailThe potentiality of PDMAEMA-based polycation for cardiovascular applications
Flebus, Luca ULg; Lombart, François ULg; Martinez, Lucia et al

Conference (2014, June 18)

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See detailIN VITRO STUDY OF A FIBER NETWORK TAILORED AS 3D-CELL MODEL
Sevrin, Chantal ULg; Lombart, François ULg; Godino, Miguel et al

Poster (2014, June 17)

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See detailPolyester-based microcarriers with enhanced surface properties for tissue engineering
Markvicheva, Elena; Privalova, Anna; Sevrin, Chantal ULg et al

Poster (2014, June 17)

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See detailPeripheral Synergism Between Tramadol and Ibuprofen in the Formalin Test
Chavarria Bolanos, Daniel ULg; Perez-Urizar, Jose; Grandfils, Christian ULg et al

in Drug Development Research (2014), 75(4), 224-230

Analgesics with different mechanisms of action can be combined in order to obtain Pharmacological synergism, employing lower doses of each agent, thus diminishing side effects. For instance, an atypical ... [more ▼]

Analgesics with different mechanisms of action can be combined in order to obtain Pharmacological synergism, employing lower doses of each agent, thus diminishing side effects. For instance, an atypical dual analgesic such as tramadol (TMD) and a nonsteroidal anti-inflammatory drug such as ibuprofen (IBU) are good candidates to be evaluated when combined and applied peripherally. The present study was conducted to evaluate possible local synergism between TMD and IBU when combined peripherally using the formalin test in rats. The effects of the individual analgesics and their combinations were evaluated simultaneously using a 5% formalin dilution. Dose–effect curves were determined for TMD (50–400 μg/paw) and IBU (1–100 μg/paw). Experimental effective doses were evaluated and isobolographic analyses were constructed to evaluate TMD-IBU combination synergism. Both drugs produced a dose-dependent analgesic effect when applied separately. Isobolographic analysis showed synergism during phase 1 (0–10 min) and phase 2 (15–60 min) when compared with theoretical doses (P < 0.05), with interaction indexes of 0.06 and 0.09, respectively. The present information supports the peripheral analgesic effect of TMD and IBU, especially when combined at appropriate doses. [less ▲]

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See detailInspired bio- nanobiomaterials for tissue engineering and drug delivery systems
Grandfils, Christian ULg

in Vth International Workshop on "NANOPARTICLES, COATINGS AND MICROCONTAINERS: TECHNOLOGY, PROPERTIES, APPLICATIONS" Conference Materials (2014, May 09)

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See detailControlled Production of Exopolysaccharides from Enterobacter A47 as a Function of Carbon Source w ith Demonstration of Their Film and Emulsifying Abilities
Freitas, F; Alves, A.V.; Gouveia, A.R. et al

in Applied Biochemistry and Biotechnology (2014), 172

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See detailImpact of glycerol and nitrogen concentration on Enterobacter A47 growth and exopolysaccharide production
Torres, Ch.; Marques, R.; Ferreira, A.R. et al

in International Journal of Biological Macromolecules (2014), 71

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See detailApplication of heparin as a dual agent with antimalarial and nanocapsule targeting activity towards Plasmodium-infected red blood cells
Marques, J.; Moles, E.; Urbán, P. et al

in Nanomedicine (2014), 10(1719–1728),

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See detailEmission Tunable, Cytocompatible, Near-IR-Emitting Ag2S Quantum Dots by Aqueous Decomposition of DMSA
Hocaoglu, I.; Demir, F.; Birer, O. et al

in Nanoscale (2014), 6

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See detailBiodegradable polyester-based microcarriers with modified surface tailored for tissue engineering
Privalova, A.; Markvicheva, E.; Sevrin, Chantal ULg et al

in Journal of Biomedical Materials Research, Part A (2014)

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