Physico-chemical characterization and in vitro hemocompatbility study of nanocarriers tailored for biopharmaceutical drugs

Conference (2012, December 04)

Introduction to advanced biomaterials

Conference (2012, November 13)

The applications of biodegradable polymers for drug delivery and tissue engineering

in Proceeding XVI International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics (2012, September 25)

In vitro hemocompatibility of nanocarriers tailored for biopharmaceutical drugs.

Poster (2012, May 06)

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed ... [more ▼]

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed the large surface they are able to expose to the biological environment promotes their interaction with various biochemicals, in particular proteins which can after adsorption elicit the activation of biological cascades either responsible from NP clearance or/and harmful body reaction (inflammatory / coagulation). In the frame of the European Integrated Project : “Nanobiopharmaceutics”, we have the opportunity to compare the hemoreactivity of about 145 different NP samples differing in core and surface chemistry and classified according to their expected difference in hydrophobicity based on the nature of their core materials. According to this classification, PLGA nanoparticles, polyglycidol-polyethyethylene oxide nanoparticles, polyglycidol thyolated or polyacrylamide nanogels, and polyelectrolyte complexes either based on polyamidoamine or poly(N,N-dimethylamino- 2-ethylmethacrylate) have been evaluated within a concentration ranging from 0.3 to 1000 μg/mL. These in vitro tests have been realized for screening purpose adopting normal human bloods and according to Iso 10993. As a summary of this extensive study, our results clearly highlight that most of the polymeric nanoparticles evaluated give rise to some alterations of the blood components. In particular the platelets, intrinsic pathway of coagulation and complement activation are the most reactive biological parameters in the presence of these nanostuctures. Although not strictly related to the surface chemistry our classification has also allowed us to derive some clear correlations between nanomaterial properties and their hemoreactivity. Within the class of polyelectrolyte electrolyte complexes, the modifications brought in the surface chemistry has drastically improved their hemoreactivity [less ▲]

Neuropeptides and endogenous opioids expression during silent chronic inflammation

Poster (2012, April 18)

Controlled synthesis of two fluorescent derivatives of poly(2-dimethyl-aminoethyl-methacrylate) adopting AtomTransfer Radical Polymerization (ATRP).

Poster (2012, April 18)

Les matériaux imiplantables : quel contrôle assurer dans l'avenir ?

Scientific conference (2012, March 20)

Optimization of biodegradable microparticles loaded with thrombin receptor agonist peptide (TRAP-6) for tissue engineering

Conference (2012)

Poly(2-dimethylamino ethylmethacrylate)-Based Polymers To Camouflage Red Blood Cell Antigens

in Biomacromolecules (2012), 13(4), 1172-1180

Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane ... [more ▼]

Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane surface of red blood cells (RBCs). The electrostatic self-assembly of PDMAEMA on RBCs membrane can be employed for inducing the formation of a polymeric shield camouflaging blood group antigens on RBCs as a valuable strategy for developing “universal RBCs” for blood transfusion. The purpose of this research was to evaluate the camouflaging ability of PDMAEMA homopolymers and PDMAEMA-copoly(nethylene glycol) copolymers differing in molecular weight and architecture. Surprisingly, the PDMAEMAs caused a partially masking, no masking, and sensitization of the same RBCs population. The MW and architecture of the polymers as well as temperature of PDMAEMA-RBCs treatment influenced the results observed. Herein, the very particular reactivity of PDMAEMAs and RBCs is analyzed and discussed. [less ▲]

Study of the interactive effect of temperature and pH on exopolysaccharide 3 production by Enterobacter A47 using multivariate statistical analysis

in Bioresource Technology (2012)