References of "Grandfils, Christian"
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See detailEffect of surface modification of polylactides on fibroblasts L-929 and osteoblasts MG-63
Tilkin, Rémi ULg; Regibeau, Nicolas ULg; Lambert, Stéphanie ULg et al

Conference (2017, July 12)

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently ... [more ▼]

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently based on the realization of temporary porous matrices, also called "scaffolds", design as model and structure for the development of cells. Surface properties of those biomaterials are some determinant criteria for cell adhesion and proliferation. The scope of this work is the study of adhesion and proliferation of fibroblasts L-929 and osteoblasts MG-63 on untreated, hydrolyzed or aminolyzed polylactides. First, these substrates are developed: poly-L,D-lactide (PDLLA) powder is pressed into disks. The surface of disks was chemically modified by hydrolysis and aminolysis surface treatments. Surface modifications were based on previous studies. [1,2] The hydrolysis process is carried out by immersing PDLLA disks in 2 M NaOH for 20 min at room temperature (24°C) under constant agitation. The aminolysis process is performed by placing PDLLA disks in 15 % ethylenediamine in isopropanol for 5 min at room temperature (24°C) under constant agitation. Substrates are characterized by optical microscopy, Scanning Electron Microscopy and water contact angle. Finally, cell adhesion, proliferation, and viability on these substrates are assessed by cell counting and MTT assay after 1, 4, and 8 days of cell culture. Results from surface characterization show an increase of roughness for hydrolyzed polylactide. Regarding water contact angle measurements, values are smaller on treated substrates even though the difference with untreated substrates appears smaller than expected in the literature. [1,2] Cell counting and MTT assay show an increase of cell proliferation and cell viability for treated polylactide substrates in the case of fibroblasts and a decrease of cell proliferation for aminolyzed polylactide substrates in the case of osteoblasts. In conclusions, this work highlights the different effects of surface modification on fibroblasts and osteoblasts viability. References : [1]. Wang YQ, Cai JY. Enhanced cell affinity of poly(L-lactic acid) modified by base hydrolysis: wettability and surface roughness at nanometer scale. Current Applied Physics 2007, 7(S1):e108–e111. [2]. Lin Y, Chrzanowski W, Knowles J, Bishop A, Bismarck A. Functionalized poly(D,L-lactide) for pulmonary epithelial cell culture. Advanced Engineering Materials 2010, 12(4): B101–B112. [less ▲]

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See detailAdaptation of targeted nanocarriers to changing requirements in antimalarial drug delivery
Marques, J.; Valle-Delgado, J.; Urbán, P. et al

in Nanomedicine : Nanotechnology, Biology, and Medicine (2017)

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See detailDevelopment of polymer-based systems for healthcare applications
Grandfils, Christian ULg

Conference (2016, October 06)

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See detailFunctional properties of extracellular polysaccharides produced by Enterobacter A47 grown on agro-food industry by-products
Antunes, Silvia; Freitas, Filomena; Alves, Vitor et al

Poster (2016, September)

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See detailFlocculation properties of the bacterial exopolysaccharide FucoPol
Araújo, Diana; Reis, Patricia; Marques, Anna et al

Conference (2016, September)

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See detailTailored made degradable synthetic polymers for 3D (bio)printing
Grandfils, Christian ULg

Conference (2016, January 26)

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See detailDPSC colonization of functionalized 3D textiles
Ortiz, Marine; Rosales-Ibáñez, R; Pozos-Guillén, A. et al

in Journal of Biomedical Materials Research, Part B : Applied Biomaterials (2016), 10.1002/jbm.b.33609

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See detailAn in vitro and in vivo study of peptide-functionalized nanoparticles for brain targeting: The importance of selective blood-brain barrier uptake.
Bode, G; Coue, G.; Freese et al

in Nanomedicine : Nanotechnology, Biology, and Medicine (2016)

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See detailDevelopment and characterization of bilayer films of FucoPol and chitosan
Ferreira, A.R.V.; Torres, C.A.V.; Freitas, F. et al

in Carbohydrate Polymers (2016), 147

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See detailAssessment of the adhesive properties of FucoPol, a bacterial polysaccharide
Araújo, D.; Alves, V.D.; Campo, J. et al

in International Journal of Biological Macromolecules (2016), 92

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See detailSynthesis of hydrophilic CuInS2/ZnS quantum dots with different polymeric shells and study of their cytotoxicity and hemocompatibility
Speranskaya, Elena Sergeevna; Sevrin, Chantal ULg; De Saeger, Sarah et al

in ACS Applied Materials and Interfaces (2016), 6

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See detailComparison of commercial membranes tailored as 3D in vitro cell models
Romano, Ilaria ULg; Tilkin, Rémi ULg; Hubaux, Roland et al

Poster (2016)

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See detailIn vitro and in vivo evaluation of drug-eluting microspheres designed for transarterial chemoembolization therapy
Wang, Y.; Molin, D.; Sevrin, Chantal ULg et al

in International Journal of Pharmaceutics (2016), 503

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See detailStudy of cell growth on/in modified polymer matrices as scaffolds for tissue engineering
Markvicheva, E.; Drozdova, M.; Demina, T. et al

Poster (2016)

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See detailPolylactide-based microspheres prepared using solid-state copolymerized chitosan and D,L-lactide
Demina, Tatiana; Akopova, T.A.; Vladimirov, L.V. et al

in Materials Science and Engineering: A (2016), C59

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