References of "Goukens, Eve"
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See detailAutomated synthesis of [18F] FBEM for labeling of thiol containing compounds
Paris, Jérôme ULg; Thonon, David ULg; Kaisin, Geoffroy ULg et al

Poster (2011, September 01)

[18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the ... [more ▼]

[18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the present work was to develop a fast, reproducible and fully automated synthesis of this compound in order to improve its availabilty as well as for obvious radioprotection matters. [less ▲]

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See detailPhotoclick chemistry: Applications to 18F radiotracers preparation
Thonon, David ULg; Goukens, Eve ULg; Kaisin, Geoffroy ULg et al

in Journal of Labelled Compounds and Radiopharmaceuticals (2011)

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See detailFully Automated Preparation and Conjugation of N-Succinimidyl 4-[(18)F]Fluorobenzoate ([ (18)F]SFB) with RGD Peptide Using a GE FASTlab Synthesizer.
Thonon, David ULg; Goblet, D.; Goukens, Eve ULg et al

in Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging (2011)

PURPOSE: The aim of this work was to automate the radiosynthesis of [(18)F]SFB, a widely used reagent for the labeling of biomolecules with (18)F on a new generation commercial synthesis module (FASTLab ... [more ▼]

PURPOSE: The aim of this work was to automate the radiosynthesis of [(18)F]SFB, a widely used reagent for the labeling of biomolecules with (18)F on a new generation commercial synthesis module (FASTLab, GE Healthcare). PROCEDURES: Two synthesis approaches were implemented on this module: the classical "two-pot radiosynthesis" and the more recently described "one-pot" method. RESULTS: The "two-pot" approach affords [(18)F]SFB with a 42% decay-corrected yield in 57 min (n = 24) with a chemical purity sufficient to avoid an intermediate HPLC purification. The recently established "one-pot" method, afforded a product with a lower chemical purity, in the conditions used in this report. The lower d.c. yield obtained (32% (n = 15)) was related to the low (18)F labeling yields obtained in MeCN compared with DMSO. The subsequent conjugation step with a RGD (PRGD2) peptide was also successfully automated. CONCLUSIONS: The formulated [(18)F]FPRGD2 was obtained without any operator manipulation with a d.c. yield of 13% +/- 3% (n = 13) in 130 min, a radiochemical purity >98% and a specific activity of 140 +/- 40 TBq/mmol. [less ▲]

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