Gene Expression Study in Positron Emission Tomography–Positive Abdominal Aortic Aneurysms Identifies CCL18 as a Potential Biomarker for Rupture Risk
Courtois, Audrey ; Nusgens-Richelle, Betty ; HUSTINX, Roland et al
in Molecular Medicine (2015)Detailed reference viewed: 21 (8 ULg)
Gene expression study in PET positive abdominal aortic aneurysms identifies CCL18 as a potential biomarker for rupture risk.
Nusgens-Richelle, Betty ; Hustinx, Roland ; et al
in Molecular medicine (Cambridge, Mass.) (2014)
Rupture of abdominal aortic aneurysm (AAA) is a cause of significant mortality and morbidity in ageing populations. Uptake of 18-fluorodeoxyglucose (FDG) detected by positron emission tomography (PET) is ... [more ▼]
Rupture of abdominal aortic aneurysm (AAA) is a cause of significant mortality and morbidity in ageing populations. Uptake of 18-fluorodeoxyglucose (FDG) detected by positron emission tomography (PET) is observed in the wall of 12% of AAA (A+), most of them being symptomatic. We previously showed that the metabolically active areas displayed adventitial inflammation, medial degeneration and molecular alterations prefacing wall rupture. The aim of this study was to identify new factors predictive of rupture.Transcriptomic analyses were performed in the media and adventitia layers from three types of samples: AAA without (A0) and with FDG uptake (A+), both at the positive spot (A+Pos) and at a paired distant negative site (A+Neg) of the same aneurysm. Follow-up studies included RT-PCR, immunohistochemical staining and ELISA. A large number of genes, including matrix metalloproteinases, collagens and cytokines as well as genes involved in osteochondral development, were differentially expressed in the A+Pos as compared to A+Neg. Moreover, a series of genes, notably CCL18, was differentially expressed both in the A+Neg and A+Pos as compared to the A0. A significant increase of CCL18 was also found at the protein level in the aortic wall and in peripheral blood of A+ patients as compared to A0.In conclusion, new factors, including CCL18, involved in the progression of AAA and, potentially, in their rupture were identified by a genome-wide analysis of PET-positive and negative human aortic tissue samples. Further work is needed to study their role in AAA destabilization and weakening. [less ▲]Detailed reference viewed: 16 (3 ULg)
INTÉRÊTS DE LA TOMOGRAPHIE À ÉMISSION DE POSITONS DANS LE SUIVI ET LE PRONOSTIC DES ANÉVRYSMES DE L’AORTE ABDOMINALE
Courtois, Audrey ; Nusgens-Richelle, Betty ; Hustinx, Roland et al
in Revue Médicale de Liège (2014)Detailed reference viewed: 19 (9 ULg)
Multifactorial Relationship Between 18F-Fluoro-Deoxy-Glucose Positron Emission Tomography Signaling and Biomechanical Properties in Unruptured Aortic Aneurysms
NCHIMI LONGANG, Alain ; CHERAMY-BIEN, Jean-Paul ; et al
in Circulation. Cardiovascular imaging (2014), 7
BACKGROUND: -The relationship between biomechanical properties and biological activities in aortic aneurysms was investigated with finite element simulations (FES) and 18F-fluoro-deoxyglucose (18F-FDG ... [more ▼]
BACKGROUND: -The relationship between biomechanical properties and biological activities in aortic aneurysms was investigated with finite element simulations (FES) and 18F-fluoro-deoxyglucose (18F-FDG) positron emission tomography (PET). METHODS AND RESULTS: -The study included 53 patients (45 males) with aortic aneurysms, 47 infrarenal (AAA) and 6 thoracic (TAA), who had at least one 18F-FDG PET/computed tomography. Over a 30-month period, more clinical events occurred in patients with increased 18F-FDG uptake on their last examination than in those without (5/18 (28%) vs. 2/35 (6%); P=0.03). Wall stress and stress/strength index computed by FES and 18F-FDG uptake were evaluating a total of 68 examinations. 25 (38%) examinations demonstrated at least one aneurysm wall area of increased 18F-FDG uptake. The mean number of these areas per examination was 1.6 (18/11) in TAAs vs. 0.25 (14/57) in AAAs, while the mean number of increased uptake areas co-localizing with highest wall stress and stress/strength index areas was 0.55 (6/11) and 0.02 (1/57), respectively. Quantitatively, 18F-FDG PET uptake correlated positively with both wall stress and stress/strength index (P<0.05). 18F-FDG uptake was particularly high in subjects with personal history of angina pectoris and familial aneurysm. CONCLUSIONS: -Increased 18F-FDG PET uptake in aortic aneurysms is strongly related to aneurysm location, wall stress as derived by FES and patient's risk factors such as acquired and inherited susceptibilities. [less ▲]Detailed reference viewed: 44 (9 ULg)
18F-FDG Uptake Assessed by PET/CT in Abdominal Aortic Aneurysms Is Associated with Cellular and Molecular Alterations Prefacing Wall Deterioration and Rupture.
Courtois, Audrey ; Richelle, Betty ; Hustinx, Roland et al
in Journal of Nuclear Medicine (The) (2013), 54
Rupture of abdominal aortic aneurysms (AAAs) leads to a significant morbidity and mortality in aging populations, and its prediction would be most beneficial to public health. Spots of positive uptake of ... [more ▼]
Rupture of abdominal aortic aneurysms (AAAs) leads to a significant morbidity and mortality in aging populations, and its prediction would be most beneficial to public health. Spots of positive uptake of 18F-FDG detected by PET are found in 12% of AAA patients (PET+), who are most often symptomatic and at high rupture risk. Comparing the 18F-FDG-positive site with a negative site from the same aneurysm and with samples collected from AAA patients with no 18F-FDG uptake should allow the discrimination of biologic alterations that would help in identifying markers predictive of rupture. METHODS: Biopsies of the AAA wall were obtained from patients with no 18F-FDG uptake (PET0, n = 10) and from PET+ patients (n = 8), both at the site of positive uptake and at a distant negative site of the aneurysmal wall. Samples were analyzed by immunohistochemistry, quantitative real-time polymerase chain reaction, and zymography. RESULTS: The sites of the aneurysmal wall with a positive 18F-FDG uptake were characterized by a strikingly increased number of adventitial inflammatory cells, highly proliferative, and by a drastic reduction of smooth muscle cells (SMCs) in the media as compared with their negative counterpart and with the PET0 wall. The expression of a series of genes involved in the maintenance and remodeling of the wall was significantly modified in the negative sites of PET+, compared with the PET0 wall, suggesting a systemic alteration of the aneurysmal wall. Furthermore, a striking increase of several matrix metalloproteinases (MMPs), notably the MMP1 and MMP13 collagenases, was observed in the positive sites, mainly in the adventitia. Moreover, PET+ patients were characterized by a higher circulating C-reactive protein. CONCLUSION: Positive 18F-FDG uptake in the aneurysmal wall is associated with an active inflammatory process characterized by a dense infiltrate of proliferating leukocytes in the adventitia and an increased circulating C-reactive protein. Moreover, a loss of SMC in the media and alterations of the expression of genes involved in the remodeling of adventitia and collagen degradation potentially participate in the weakening of the aneurysmal wall preceding rupture. [less ▲]Detailed reference viewed: 103 (40 ULg)
High Levels of 18F-FDG Uptake in Aortic Aneurysm Wall are Associated with High Wall Stress
; ; NCHIMI LONGANG, Alain et al
in European Journal of Vascular and Endovascular Surgery (2010), 39Detailed reference viewed: 106 (14 ULg)
Functional imaging of abdominal aortic aneurysms : can it predict probability of rupture.
Sakalihasan, Natzi ; Hustinx, Roland ; et al
in VASCULAR ANEURYSMS (2009)Detailed reference viewed: 21 (6 ULg)
Evaluation of inflammatory cells in abdominal aortic aneurysmal wall by tomography emission positron
Sakalihasan, Natzi ; Hustinx, Roland ; et al
in Médecine Nucléaire : Imagerie Fonctionnelle et Métabolique (2009), 33/5Detailed reference viewed: 18 (11 ULg)
Can positron emission tomography (PET) predict the risk of rupture of abdominal aortic aneurysm (AAA) ? Controversies and updates in vascular surgery.
SAKALIHASAN, Natzi ; HUSTINX, Roland ; et al
in Can positron emission tomography (PET) predict the risk of rupture of abdominal aortic aneurysm (AAA) ? Controversies and updates in vascular surgery. (2008)Detailed reference viewed: 13 (1 ULg)
Can Positron Emission Tomography (PET) predict the risk of rupture of abdominal aortic aneurysm(AAA)?
Sakalihasan, Natzi ; Hustinx, Roland ; et al
in Controversies and updates in vascular surgery 2008 (2008)Detailed reference viewed: 26 (4 ULg)
Metabolic Assessment of Abdominal Aortic Aneurysms
NCHIMI LONGANG, Alain ; BRISBOIS, Denis ; GRAYET, Benjamin et al
Poster (2004)Detailed reference viewed: 14 (1 ULg)