References of "Gogev, S"
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See detailIntraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle
Muylkens, Benoît ULg; Meurens, F.; Schynts, F. et al

in Journal of General Virology (2006), 87(Pt 8), 2149-2154

Vaccines used in control programmes of Bovine herpesvirus 1 (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are obtained ... [more ▼]

Vaccines used in control programmes of Bovine herpesvirus 1 (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are obtained at high frequency in experimentally coinfected cattle, the consequences of recombination on the virulence of gE-negative BoHV-1 were investigated. Thus, gE-negative BoHV-1 recombinants were generated in vitro from several virulent BoHV-1 and one mutant BoHV-1 deleted in the gC and gE genes. Four gE-negative recombinants were tested in the natural host. All the recombinants were more virulent than the gE-negative BoHV-1 vaccine and the gC- and gE-negative parental BoHV-1. The gE-negative recombinant isolated from a BoHV-1 field strain induced the highest severe clinical score. Latency and reactivation studies showed that three of the recombinants were reexcreted. Recombination can therefore restore virulence of gE-negative BoHV-1 by introducing the gE deletion into a different virulence background. [less ▲]

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See detailRecombination in the alphaherpesvirus bovine herpesvirus 1
Thiry, Etienne ULg; Muylkens, Benoît ULg; Meurens, F. et al

in Veterinary Microbiology (2006), 113(3-4), 171-177

Herpesviruses are DNA viruses characterized by a low rate of nucleotide substitution. Therefore. other mechanisms must be involved to their evolution, like recombination that can be seen as an essential ... [more ▼]

Herpesviruses are DNA viruses characterized by a low rate of nucleotide substitution. Therefore. other mechanisms must be involved to their evolution, like recombination that can be seen as an essential evolutionary driving force of these viruses. Recombination contributes to the long-term evolution of alphaherpes viruses. It acts also to continuously create new alphaherpesvirus strains. We have used bovine herpesvirus 1 to investigate recombination both within DNA concatemers in infected cells and in vitro and in vivo at the end of the lytic cycle. The following results have been obtained: (i) intramolecular recombination occurs at the level of concatemers and gives rise to genomic segment inversions: (ii) intraspecific recombination occurs frequently both in vitro and in vivo; (iii) interspecific recombination is possible and requires two highly genetically related viruses (iv) only simultaneous or closely separated infections lead to the production of recombinant viruses: (v) recombination between wild-type and glycoprotein defective vaccine virus can produce a glycoprotein defective virus keeping part of the virulence of parental wild-type virus. Recombination, by exchanging genomic segments, may modify the virulence of alphaherpesviruses. It must be carefully assessed for the biosafety of antiviral therapy, alphaherpesvirus-based vectors and live attenuated vaccines. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailRecombination in alphaherpesviruses
Thiry, Etienne ULg; Meurens, F.; Muylkens, Benoît ULg et al

in Reviews in Medical Virology (2005), 15(2, Mar-Apr), 89-103

Within the Herpesviridae family, Alphaherpesvirinae is an extensive subfamily which contains numerous mammalian and avian viruses. Given the low rate of herpesvirus nucleotide substitution, recombination ... [more ▼]

Within the Herpesviridae family, Alphaherpesvirinae is an extensive subfamily which contains numerous mammalian and avian viruses. Given the low rate of herpesvirus nucleotide substitution, recombination can be seen as an essential evolutionary driving force although it is likely underestimated. Recombination in alphaherpesviruses is intimately linked to DNA replication. Both viral and cellular proteins participate in this recombination-dependent replication. The presence of inverted repeats in the alphaherpesvirus genomes allows segment inversion as a consequence of specific recombination between repeated sequences during DNA replication. High molecular weight intermediates of replication, called concatemers, are the site of early recombination events. The analysis of concatemers, from cells coinfected by two distinguishable alphaherpesviruses provides an efficient tool to study recombination without the bias introduced by invisible or non-viable recombinants, and by dominance of a virus over recombinants. Intraspecific recombination frequently occurs between strains of the same alphaherpesvirus species. Interspecific recombination depends on enough sequence similarity to enable recombination between distinct alphaherpesvirus species. The most important prerequisite for successful recombination is coinfection of the individual host by different virus strains or species. Consequently the following factors affecting the distribution of different viruses to shared target cells need to be considered: dose of inoculated virus, time interval between inoculation of the first and the second virus, distance between the marker mutations, genetic homology, virulence and latency. Recombination, by exchanging genomic segments, may modify the virulence of alphaherpesviruses. It must be carefully assessed for the biosafety of antiviral therapy, alphaherpesvirus-based vectors and live attenuated vaccines. Copyright (C) 2004 John Wiley Sons, Ltd. [less ▲]

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See detailInterspecific recombination between two ruminant alphaherpesviruses, bovine herpesviruses 1 and 5
Meurens, F.; Keil, G. M.; Muylkens, Benoît ULg et al

in Journal of Virology (2004), 78(18), 9828-9836

Homologous recombination between different species of alphaherpesviruses has been described between herpes simplex viruses 1 and 2 but has not yet been observed between other alphaherpesviruses. In the ... [more ▼]

Homologous recombination between different species of alphaherpesviruses has been described between herpes simplex viruses 1 and 2 but has not yet been observed between other alphaherpesviruses. In the present study we chose to assess to what extent in vitro recombination can occur between members of a well-defined group of closely related viruses such as ruminant alphaherpesviruses. At 24 h after infection of epithelial bovine kidney cells with a double-deleted mutant of bovine herpesvirus 1 (BoHV-1) (containing green fluorescent protein and red fluorescent protein genes) and different ruminant alphaherpesviruses, four types of progeny viruses were detected and distinguished according to their phenotype. Frequent recombination events between identical or different strains of BoHV-1 were observed (up to 30%), whereas only two BoHV-1/BoHV-5 recombinants were identified, and no recombinants between BoHV-1 and less closely related caprine and cervine herpesviruses were detected. Restriction analysis of the genomes of the two BoHV-1/BoHV-5 recombinants showed different genetic backgrounds. One possessed a restriction pattern close to BoHV-1, whereas the other one was close to BoHV-5. This exhaustive analysis of each combination of coinfection in a unique situation of five closely related alphaherpesviruses revealed the importance of a high degree of genetic relatedness and similar parental virus growth kinetics for successful interspecific recombination. [less ▲]

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See detailGlycol chitosan improves the efficacy of intranasally administrated replication defective human adenovirus type 5 expressing glycoprotein D of bovine herpesvirus 1
Gogev, S.; de Fays, K.; Versali, Marie-France ULg et al

in Vaccine (2004), 22(15-16), 1946-1953

The ability of two soluble formulations, namely chitosan and glycol chitosan, when used as an intranasal adjuvant, to improve the immunogenicity of an intranasal human adenovirus type 5 replication ... [more ▼]

The ability of two soluble formulations, namely chitosan and glycol chitosan, when used as an intranasal adjuvant, to improve the immunogenicity of an intranasal human adenovirus type 5 replication defective expressing bovine herpesvirus 1 (BoHV-1) glycoprotein D based vaccine, was investigated in cattle. Their adjuvant effects on immune response by increasing clinical and especially virological protection against an intranasal BoHV-1 challenge were then evaluated. The best virological protection was obtained in calves immunized with the vaccine vector adjuvanted with glycol chitosan which decreased the challenge BoHV-1 virus excretion titres by 0.5-1.5 log when compared to those obtained in calves immunized with the vaccine vector alone or adjuvanted with chitosan. A slight difference in clinical scores was observed in calves immunized with the adjuvanted vaccine vector compared to calves immunized with the vaccine vector alone. The obtained data suggest that the tested soluble formulation of glycol chitosan has promising potential use as an intranasal adjuvant for recombinant viral vector vaccines in cattle. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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See detailBovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5
Gogev, S.; Georgin, J. P.; Schynts, F. et al

in Veterinary Research (2004), 35(6, Nov-Dec), 715-721

Bovine herpesvirus 1 glycoprotein D (gD) gene expression by recombinant replication defective human adenovirus type 5 (HAdV-5) was investigated in calves using indirect immunofluorescence microscopy (IIFM ... [more ▼]

Bovine herpesvirus 1 glycoprotein D (gD) gene expression by recombinant replication defective human adenovirus type 5 (HAdV-5) was investigated in calves using indirect immunofluorescence microscopy (IIFM), confocal laser scanning microscopy (CLSM) and RT-PCR. One fold intranasal instillation of HAdV-5-expressing gD in the cattle upper respiratory tract showed a short term expression of at least 5 days, but not 10 days, limited only to epithelial cells localised in the epithelium of the nasal mucosa in one out of six calves. Observed limited gene transfer into well differentiated cattle airway epithelial cells must be taken into consideration in order to enhance transfection efficiency, and consequently the vaccine potential of this vector. [less ▲]

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See detailBiosafety of Herpesvirus Vectors
Gogev, S.; Schynts, F.; Meurens, F. et al

in Current Gene Therapy (2003), 3(6), 597-611

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in ... [more ▼]

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in which the viral genome exists as a stable episome in the nucleus. For gene therapy to become a potential future treatment option, biosafe therapeutically efficient gene transfer is a central, but more and more stringent requirement. This review highlights the progress in development of herpesvirus based vectors, describes their properties as wall as discusses the biosafety concerns that are associated with their use in gene therapy. Thought was also given to biosafety issues pertaining to design and production of herpesvirus vector systems in therapeutic gene delivery. [less ▲]

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See detailLes chitosanes – nouveaux adjuvants pour la vaccination par voie muqueuse chez les animaux
Gogev, S.; Versali, Marie-France ULg; Thiry, Etienne ULg

in Annales de Médecine Vétérinaire (2003), 147(5, OCT-NOV), 343-350

The advantage of mucosal vaccination is the induction of an immune response at entry sites of pathogens. Because vaccines alone are poorly bioavailable after mucosal administration, they need to be co ... [more ▼]

The advantage of mucosal vaccination is the induction of an immune response at entry sites of pathogens. Because vaccines alone are poorly bioavailable after mucosal administration, they need to be co-administered with penetration enhancers, or adjuvants. Numerous studies have demonstrated that chitosans and their derivatives are safe and effective mucosal absorption enhancers of hydrophylic macromolecules such as peptides and proteins. Chitosan is a cationic polysaccharide derived from chitin present in the covering layer of arthropods and in the cell walls of many fungi. Association of vaccines to chitosans, their derivatives or some of their particulate systems, such as nano- and microparticles, has also shown to enhance antigen uptake by mucosal lymphoid tissues, thereby inducing mucosal and systemic immune responses against these antigens. Chitosan and its derivatives are promising adjuvants for mucosal vaccine delivery in animals. [less ▲]

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See detailDemonstration of generalized infection with caprine herpesvirus 1 diagnosed in an aborted caprine fetus by PCR
Keuser, V.; Gogev, S.; Schynts, F. et al

in Veterinary Research Communications (2002), 26(3), 221-226

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See detailInduction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD
Gogev, S.; Vanderheijden, N.; Lemaire, Mylène et al

in Vaccine (2002), 20(9-10), 1451-1465

Replication-defective human adenoviruses type 5 (HAd5) expressing the bovine herpesvirus type 1 (BHV-1) glycoprotein gC or gD under the control of the human cytomegalovirus immediate-early promoter ... [more ▼]

Replication-defective human adenoviruses type 5 (HAd5) expressing the bovine herpesvirus type 1 (BHV-1) glycoprotein gC or gD under the control of the human cytomegalovirus immediate-early promoter/enhancer (AdCMVgC or AdCMVgD) or the 5' regulatory region of the human desmin gene (AdDESMgC or AdDESMgD) were generated. A preliminary experiment performed on rabbits showed that the intranasal administration of AdCMV elicited higher levels of BHV-1 neutralizing antibodies than the intramuscular administration of AdDESM. The obtained results allowed to select the replication-defective AdCMVgC and AdCMVgD for further assessment of their potential as a recombinant vaccine in cattle. Calves were injected intranasally twice 3 weeks apart with either AdCMVgC or AdCMVgD or a combination of these two recombinants or a commercially available live vaccine for comparison. The highest BHV-1 neutralizing antibody titres were obtained with AdCMVgD followed by the live vaccine and to a lower extent with the combination of the two recombinants (AdCMVgC+AdCMVgD). Calves were protected against intranasal BHV-1 challenge performed 3 weeks after the second immunization. In view of the obtained results, recombinant HAd5 may be developed as an intranasal vaccine vector in cattle administrated either alone or sequentially with non-human adenovirus-based vectors. [less ▲]

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See detailPrevalence of Antibodies to Human Adenovirus Type 5 in Belgian Cattle
Gogev, S.; Lemaire, Mylène; Thiry, Etienne ULg

in Veterinary Record : Journal of the British Veterinary Association (2001), 148(24), 752-4

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See detailLes adénovirus recombinants comme vecteurs vaccinaux
Gogev, S.; Thiry, Etienne ULg

in Annales de Médecine Vétérinaire (1999), 143

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