References of "Gofflot, Sébastien"
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See detailDynamic single-cell analysis of Saccharomyces cerevisiae under process perturbation: comparison of different methods for monitoring the intensity of population heterogeneity
Delvigne, Frank ULg; Baert, Jonathan ULg; Gofflot, Sébastien et al

in Journal of Chemical Technology & Biotechnology (in press)

BACKGROUND: Single cell biology has attracted a lot of attention these past few years and has led to numerous fundamental results pointing out the heterogeneity of clonal cell populations. In this context ... [more ▼]

BACKGROUND: Single cell biology has attracted a lot of attention these past few years and has led to numerous fundamental results pointing out the heterogeneity of clonal cell populations. In this context, microbial phenotypic heterogeneity under bioprocessing conditions needs to be further investigated. In this study, yeast based processes have been investigated by using on-line flow cytometry in combination with a fluorescent transcriptional reporter (GFP) and viability fluorescence tags (propidium iodide, PI). Methods aiming at expressing the dispersion of these fluorescence tags among the yeast populations have been investigated for different bioreactor operating conditions. RESULTS: Yeast viability was determined on the basis of PI uptake. Segregation between PI negative and positive subpopulations could be efficiently quantified on the basis of the mean-to-median ratio or the amplitude of the interquartile range. On the other hand, the same quantification could not be made for the segregation occurring at the level of GFP synthesis. Indeed, when cells were exposed to sub-lethal or mild stresses (such as in scale-down reactors) two GFP subpopulations could be visualized by real-time FC, but quantification by one of the above-mentioned methods was not possible. CONCLUSIONS: Yeast population heterogeneity was observed in representative bioreactor operating conditions. Difficulties for the determination of segregation at the level of GFP synthesis point out the fact that one needs to understand the segregation mechanisms for the applied fluorescent reporters, to judge whether simple mathematical tools may be applied or if more sophisticated computational tools are needed for the quantification of the microbial population segregation. [less ▲]

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See detailA fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Gofflot, Sebastien et al

in Applied Microbiology and Biotechnology (2015)

Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state related physiological mechanisms. This work is based on the comparative analysis of classical ... [more ▼]

Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state related physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a Gla::GFP fusion protein by Aspergillus oryzae. The biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. Since the production of the target protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, the biofilm mode of culture is expected to enhance the global productivity. Although production of the target protein was enhanced by using the biofilm mode of culture, we also found that fusion protein production is also significant when the submerged mode of culture is used. This result is related to high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights the preservation of fusion protein integrity produced in biofilm conditions. Two fungal biofilm reactor designs were then investigated further, i.e. with full immersion of the packing or with medium recirculation on the packing, and the scale-up potentialities were evaluated. In this context, it has been shown that full immersion of the metal packing in the liquid medium during cultivation allows for a uniform colonization of the packing by the fungal biomass and leads to a better quality of the fusion protein. [less ▲]

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See detail4. Qualité des froments en 2014: une récolte prometteuse et puis la douche froide
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Bodson, Bernard; Destain, Jean-Pierre (Eds.) Livre Blanc Céréales (2014, September 11)

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See detail2. Variétés - 1. Froment d'hiver
Meza Morales, Walter ULg; Couveur, Luc; Eylenbosch, Damien ULg et al

in Bodson, Bernard; Destain, Jean-Pierre (Eds.) Livre Blanc Céréales (2014, September 11)

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See detailVariétés - 1. Froment d'hiver
Meza Morales, Walter; Mahieu, O.; Heens, Benoît et al

in Destain, Jean-Pierre; Bodson, Bernard (Eds.) Livre Blanc céréales (2013, September 12)

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See detail4. Froment: premiers résultats de la récolte 2012
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Destain, Jean-Pierre; Bodson, Bernard (Eds.) Livre Blanc - Céréales - Gembloux (2012, September 06)

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See detail2. Variétés - 1. Froment d'hiver
Seutin, Benoit ULg; Jacquemin, Guillaume; Vancutsem, Françoise ULg et al

in Destain, Jean-Pierre; Bodson, Bernard (Eds.) Livre Blanc - Céréales - Gembloux (2012, September 06)

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See detail4. Froment 2011: une récolte disparate
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Livre Blanc: Céréales-Gembloux - Informations avant les semis (2011, September 08)

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See detail2. Variétés - 1. Froment d'hiver
Seutin, Benoit ULg; Vancutsem, Françoise ULg; Couvreur, Luc et al

in Livre blanc: Céréales-Gembloux - Informations avant les semis (2011, September 08)

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See detailVariétés - 1. Froment d'hiver
Seutin, Benoit ULg; Vancutsem, Françoise ULg; Couvreur, Luc et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2010, September 09)

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See detailFroment 2010 : une réédition de 2006 ?
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2010, September 09)

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See detailFroment 2009: de bons rendements mais une piètre qualité boulangère
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2009, September 10)

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See detail4. 2008: Une récolte longue et difficile!
Sinnaeve, Georges; Gofflot, Sébastien; Chandelier, Anne et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2008, September 11)

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