Fast and high yield recovery of arabinose from destarched wheat bran

in Industrial Crops & Products (2013), 43

Enzymatically destarched wheat bran (DWB) contained 13.8% of arabinose and 23.1% xylose. Up to a maximum of 70% of the arabinose was progressively released from DWB when heated at 80 or 100°C in media ... [more ▼]

Enzymatically destarched wheat bran (DWB) contained 13.8% of arabinose and 23.1% xylose. Up to a maximum of 70% of the arabinose was progressively released from DWB when heated at 80 or 100°C in media acidified with HCl. Whereas microwave irradiation at higher temperatures in pressure vessels could lead to higher yields of extraction. A Box-Behnken experimental design established an efficient model describing the effects of temperature, irradiation duration and pH on arabinose extraction. The pH appeared as the most important factor of the process. 4-5 min of microwave heating at 150ºC and pH 1 appeared as a fast and highly efficient method to recover more than 90% of the arabinose of DWB. When plotting the percentages of arabinose against the combined severity factors LogR’0 (calculated from the temperature/duration/pH conditions applied), two different fitting profiles were obtained for both the heating techniques. Under microwave heating, high free xylose’s release could also occur. The experimental design led to a quadratic model predicting the release of xylose from DWB. A range of conditions enabled to minimize xylose and hydrolyze around 50% of the total arabinose, yielding a high purity fraction. An alternative would be to release more than 90% of both arabinose and xylose, for further arabinose purification or for a common valorization of both pentoses. [less ▲]

In vitro determination of prebiotic potential of sugar beet pulp extracted candidates and influence of production method

Poster (2012, June 12)

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol ... [more ▼]

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol precipitation. Then two technics are used to produced pectic oligosaccharides (POS) : enzymatic hydrolysis (EnzPOS : Rapidase Smart (DSM) ; 50°C ; pH 5) and microwaves-assisted hydrolysis (MW1POS : 104°C/10min/pH 7; MW2POS : 110°C/15min/pH 7). Structural characterization of fractions gave rise to different structural profiles between the four products. Moreover, fermentation parameters obtained in-vitro (A, B, Rmax and Tmax) were also characteristic of the fractions and in favor of a tight relationship between POS structure and POS function. Finally, to avoid solvent use, POS production could be envisaged directly on SBP. Avoiding the step of acidic extraction will allow to meet the green chemistry principles. [less ▲]

Rapport scientifique et technique de mi-convention pour l'évaluation intermédiaire du projet HYDRASANTE

Report (2012)

In vitro evaluation of the prebiotic potential of new carbohydrates extracted from agro-industrial byproducts

Poster (2011, November 16)

Les Aliments Fonctionnels

Scientific conference (2011, November 03)

Enzymatic production of pectic oligosaccharides from polygalacturonic acid with commercial pectinase preparations

in Food and Bioproducts Processing: Transactions of the Institution of Chemical Engineers, Part C (2011), 90(3), 588-596

The present study investigates the individual efficiency of six commercial pectinase preparations (Endopolygalacturonase M2, Pectinase, Viscozyme L, Pectinex Ultra SP-L, Pectinase 62L and Macer8 FJ) in ... [more ▼]

The present study investigates the individual efficiency of six commercial pectinase preparations (Endopolygalacturonase M2, Pectinase, Viscozyme L, Pectinex Ultra SP-L, Pectinase 62L and Macer8 FJ) in catalyzing the liberation of pectic oligosaccharides (POS) from polygalacturonic acid. On the basis of high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis of the enzymatic hydrolysates, products release kinetics revealed a random cleavage pattern and an exo mode of cleavage for all the enzymes except for Endopolygalacturonase M2. All six enzymes generated oligoGalA with different degree of polymerization (DP); the quantitative composition of oligoGalA depended on the enzyme specificity and the time of enzymatic reaction. Endopolygalacturonase M2 was the best enzyme preparation for production of oligoGalA, with 18% (wt) of digalacturonic acid and 58% (wt) of trigalacturonic acid after 2h of reaction. Concerning galacturonic acid production, Pectinase 62L was superior to the other enzyme preparations with 47% (wt) after 1h of reaction. [less ▲]

Rapport 1 FIRST Spin Off IMONIC

Report (2011)

A method inspired by nature for the production of original and purified prebiotic and calcium fortified preparations containing isomaltooligosaccharides and gluconic acid.

Conference (2011, June 16)

A method inspired by nature for the production of original and purified prebiotic and calcium fortified preparations containing isomaltooligosaccharides and gluconic acid.

Poster (2011, June 15)

A method inspired by nature for the production of original and purified prebiotic preparations containing isomaltooligosaccharides and gluconic acid.

Poster (2011, April 29)