Showing results 1 to 20 of 22
 Topical application of liposomes: parameters influencing their effectivenessGillet, Aline  ; Lecomte, Frédéric ; Hubert, Pascale et alPoster (2011, June 14) Detailed reference viewed: 13 (3 ULg) Liposome surface charge influence on skin penetration behaviourGillet, Aline ; Compère, Philippe ; Lecomte, Frédéric et alin International Journal of Pharmaceutics (2011), 411(1-2), 223-231 Detailed reference viewed: 45 (17 ULg)Parameters influencing the skin penetration effectiveness of liposomesGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alConference (2011, May 12) Detailed reference viewed: 18 (4 ULg)Evaluation of parameters affecting skin penetration behaviour of liposomesGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alPoster (2011, March 28) Detailed reference viewed: 17 (1 ULg) Liposomes and parameters affecting their skin penetration behaviourGillet, Aline ; Evrard, Brigitte ; Piel, Géraldine in Journal of Drug Delivery Science and Technology [=JDDST] (2011), 21 Detailed reference viewed: 75 (22 ULg)Nuclear delivery of a therapeutic peptide by long circulating pH-sensitive liposomes: Benefits over classical vesicles.Ducat, Emilie ; Deprez, Julie ; Gillet, Aline et alin International Journal of Pharmaceutics (2011) The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes ... [more ▼] The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes [SPC:CHOL:PEG-750-DSPE (47:47:6 molar% ratio)] or pH-sensitive stealth liposomes [DOPE:CHEMS:CHOL:PEG(750)-DSPE (43:21:30:6 molar% ratio)] were used to deliver a therapeutic peptide to its nuclear site of action. The benefit of using stealth pH-sensitive liposomes was investigated and formulations were compared to classical liposomes in terms of size, shape, charge, encapsulation efficiency, stability and, most importantly, in terms of cellular uptake. Confocal microscopy and flow cytometry were used to evaluate the intracellular fate of liposomes themselves and of their hydrophilic encapsulated material. Cellular uptake of peptide-loaded liposomes was also investigated in three cell lines: Hs578t human epithelial cells from breast carcinoma, MDA-MB-231 human breast carcinoma cells and WI-26 human diploid lung fibroblast cells. The difference between formulations in terms of peptide delivery from the endosome to the cytoplasm and even to the nucleus was investigated as a function of time. Characterization studies showed that both formulations possess acceptable size, shape and encapsulation efficiency but cellular uptake studies showed the important benefit of the pH-sensitive formulation over the classical one, in spite of liposome PEGylation. Indeed, stealth pH-sensitive liposomes were able to deliver hydrophilic materials strongly to the cytoplasm. Most importantly, when encapsulated in pH-sensitive stealth liposomes, the peptide was able to reach the nucleus of tumorigenic and non tumorigenic breast cancer cells. [less ▲] Detailed reference viewed: 31 (24 ULg)Skin penetration behaviour of liposomes as a function of their compositionGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alin European Journal of Pharmaceutics & Biopharmaceutics (2011), 79 Detailed reference viewed: 51 (35 ULg)Interest of vesicular systems in dermal drug deliveryGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alPoster (2010, October) Detailed reference viewed: 15 (3 ULg)Penetration mechanism of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alPoster (2010, July 06) Detailed reference viewed: 34 (9 ULg)Suggested mechanism of action of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alConference (2010, April 09) Detailed reference viewed: 48 (16 ULg)Classical and deformable liposomes: possible mechanism of penetration through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2010, March 29) Detailed reference viewed: 38 (7 ULg)Suggested mechanism of action of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alin Journal of Pharmacy & Pharmacology (2010), 62(6), 788-807 Detailed reference viewed: 52 (12 ULg)Development of a new topical system: Drug-in-cyclodextrin-in-deformable liposomeGillet, Aline ; Grammenos, Angeliki ; Compère, Philippe et alin International Journal of Pharmaceutics (2009), 380(1-2), 174-180 A new delivery system for cutaneous administration combining the advantages of cyclodextrin inclusion complexes and those of deformable liposomes was developed, leading to a new concept: drug ... [more ▼] A new delivery system for cutaneous administration combining the advantages of cyclodextrin inclusion complexes and those of deformable liposomes was developed, leading to a new concept: drug-incyclodextrin-in-deformable liposomes. Deformable liposomes made of soybean phosphatidylcholine (PC) or dimyristoylphosphatidylcholine (DMPC) and sodium deoxycholate as edge activator were compared to classical non-deformable liposomes. Liposomes were prepared by the film evaporation method. Betamethasone, chosen as the model drug,was encapsulated in the aqueous cavity of liposomes by the use of cyclodextrins. Cyclodextrins allowan increase in the aqueous solubility of betamethasone and thus, the encapsulation efficiency in liposome vesicles. Liposome size, deformability and encapsulation efficiency were calculated. The best results were obtained with deformable liposomes made of PC in comparison with DMPC. The stability of PC vesicles was evaluated by measuring the leakage of encapsulated calcein on the one hand and the leakage of encapsulated betamethasone on the other hand. In vitro diffusion studies were carried out on Franz type diffusion cells through polycarbonate membranes. In comparison with non-deformable liposomes, these new vesicles showed improved encapsulation efficiency, good stability and higher in vitro diffusion percentages of encapsulated drug. They are therefore promising for future use in ex vivo and in vivo experiments. [less ▲] Detailed reference viewed: 87 (28 ULg)Betamethasone in cyclodextrin in deformable liposomes for dermal applications: ex vivo diffusion studiesGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alConference (2009, June 09) Detailed reference viewed: 23 (6 ULg)Betamethasone in cyclodextrin in deformable liposomes for dermal applications: ex vivo diffusion studiesGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, June 07) Detailed reference viewed: 16 (2 ULg)Betamethasone in cyclodextrin in deformable liposomes for cutaneous applications: development and diffusion studiesGillet, Aline ; Grammenos, Angeliki ; Lecomte, Frédéric et alConference (2009, May 14) Detailed reference viewed: 20 (2 ULg)Betamethasone in cyclodextrin in deformable liposomes for topical applications: absorption studies using pig skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, April 01) Detailed reference viewed: 16 (3 ULg)Development of a new cutaneous delivery system: drug in cyclodextrin in deformable liposomesGillet, Aline ; Grammenos, Angeliki ; Evrard, Brigitte et alPoster (2009, March 09) Detailed reference viewed: 6 (2 ULg)development and validation of a SPE-LC-UV method for the determination of betamethasone in pig skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, March 09) Detailed reference viewed: 28 (6 ULg)Mise au point de liposomes déformables contenant de la bétamethasone encapsulée sous forme de complexes dans des cyclodextrines dans le but d'une application dermatologiqueGillet, Aline ; Grammenos, Angeliki ; Evrard, Brigitte et alConference (2009, February 03) Detailed reference viewed: 8 (3 ULg)
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