References of "Gharbi, Myriam"
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See detailStudy on the effects of laminarin, a polysaccharide from seaweed, on gut characteristics
Deville, Christelle ULg; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

in Journal of the Science of Food and Agriculture (2007), 87(9), 1717-1725

This study investigates whether laminarin (beta 1-3,beta 1-6-glucan), a polysaccharide from seaweed, exhibits beneficial properties for human health by analysing its effects on intestinal parameters ... [more ▼]

This study investigates whether laminarin (beta 1-3,beta 1-6-glucan), a polysaccharide from seaweed, exhibits beneficial properties for human health by analysing its effects on intestinal parameters. Anaerobic batch culture fermenters were used for the screening of the in vitro utilization of laminarin by the human gut microflora through the monitoring of biochemical and microbiological parameters. Additionally, the influence of laminarin ingestion on the composition of intestinal mucus (neutral mucins, sialomucins and sulphomucins) was studied in rats. Laminarin was almost totally (more than 90% used) fermented after 24 h of incubation with human intestinal bacteria. It was not selectively used by bifidobacteria and lactobacilli, but increased the production of propionate and butyrate. Variations of mucus composition were observed in jejunum, ileum, caecum and colon, both in lumen content and in intestinal wall, of rats after ingestion of this polysaccharide. Due to its effects on mucus composition, laminarin could influence the adherence and the translocation of bacteria across the epithelial wall. In conclusion, laminarin seems to be a modulator of the intestinal metabolism by its effects on mucus composition, intestinal pH and short chain fatty acid (SCFA) production, especially butyrate. (c) 2007 Society of Chemical Industry. [less ▲]

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See detailModulation of intestinal urea cycle by dietary spermine in suckling rat
Gharbi, Myriam ULg; Powroznik, Brigitte; Mazzucchelli, Gabriel ULg et al

in Biochemical and Biophysical Research Communications (2005), 336(4), 1119-1124

Argininosuccinate synthetase, an ubiquitous enzyme in mammals, catalyses the formation of argininosuccinate, the precursor of arginine. Arginine is recognised as an essential amino acid in foetuses and ... [more ▼]

Argininosuccinate synthetase, an ubiquitous enzyme in mammals, catalyses the formation of argininosuccinate, the precursor of arginine. Arginine is recognised as an essential amino acid in foetuses and neonates, but also as a conditionally essential amino acid in adults. Argininosuccinate synthetase is initially expressed in enterocytes during the developmental period, it disappeared from this organ then appeared in the kidneys. Although the importance of both intestinal and renal argininosuccinate synthetases has been recognised for a long time, nutrients have not yet been identified as inducers of the gene expression. In the context of a proteomic screening of intestinal modifications induced by dietary spermine in suckling rats, we showed that argininosuccinate synthetase and carbamoyl phosphate synthase disappeared from enterocytes after this treatment. The disappearance of argininosuccinate synthetase in small intestine was confirmed by immunodetection. Expression of carbamoyl phosphate synthase and argininosuccinate synthetase coding genes decreased also after spermine administration. Expression of other urea cycle enzyme coding genes was modulated by spermine administration: argininosuccinate lyase decreased and arginase increased. Our results fit with the developmental variation of argininosuccinate synthetase and carbamoyl phosphate synthase. Modulation of the gene expression for several urea cycle enzymes suggests a coordination between all the pathway steps and switch toward polyamine (or proline and glutamate) biosynthesis from ornithine. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailDifferential effect of dietary spermine on alkaline phosphatase activity in jejunum and ileum of unweaned rats
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Biochimie (2004), 86(7), 487-493

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas ... [more ▼]

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas, and small intestine. We showed that this polyamine modulates differently the activity of alkaline phosphatase in jejunum and ileum in suckling rat. In 14-day-old rat which had received spermine orally for 3 days, once daily, an increase of alkaline phosphatase activity in the jejunum and a decrease of this activity in the ileum was observed. Alkaline phosphatase was located at the bottom of the villus in the control jejunum and in the whole length of the villus in spermine-treated rats. On the contrary, in ileum of controls, this enzyme was present in the whole length of the villus but disappeared in the spermine-treated animals. An enzyme mass shift was observed in the small intestine after spermine administration. Spermine administration did not change the expression of genes coding for alkaline phosphatase, suggesting a post-transcriptional modification. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]

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See detailPotentialisation de l'immunité naturelle du rat nouveau-né par les polyamines exogènes
Powroznik, Brigitte; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

Conference (2004)

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See detailPolyamine as innate immunity modulator
Peulen, Olivier ULg; Powroznik, Brigitte; Gharbi, Myriam ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 449

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See detailSpermine-induced modifications of intestinal urea cycle
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Pflügers Archiv : European Journal of Physiology (2004)

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See detailEnhancement of lysozyme stability and activity by polyamines
Powroznik, Brigitte; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

in Biochimie (2004), 86(9-10, Sep-Oct), 651-656

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In ... [more ▼]

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In this report, we analysed the binding of spermine to lysozyme and its effect on thermal inactivation of the protein by spectroscopy techniques. The activity of the enzyme was analysed in presence of spermine by lysoplate technique. We studied the effects of spermine ingestion by suckling rats on intestinal lysozyme activity and gene expression. We reported that spermine binds to lysozyme and increases in vitro the thermal stability and the activity of the protein. When administered orally to suckling rats, spermine increases the lysozyme activity in jejunum, but not in ileum. This increase is not due to a modification of the gene expression. The observed effects lead us to postulate that spermine could be used in some mammals as a promoter of the innate immunity. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]

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