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See detailEnzymatic synthesis of glycosylated compounds in non-conventional media
Galonde, Nadine ULg

Doctoral thesis (2013)

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See detailUse of response surface methodology for the optimization of the lipase-catalyzed synthesis of mannosyl myristate in pure ionic liquid
Galonde, Nadine ULg; Brostaux, Yves ULg; Richard, Gaetan ULg et al

in Process Biochemistry (2013), 48(11), 1914-1920

The purpose of the present study is to find the conditions allowing to reach the highest 24 h-yield(24 h- ) for the synthesis of mannosyl myristate catalyzed by the immobilized lipase B from Candida ... [more ▼]

The purpose of the present study is to find the conditions allowing to reach the highest 24 h-yield(24 h- ) for the synthesis of mannosyl myristate catalyzed by the immobilized lipase B from Candida antarctica (Novozym®435) in the ionic liquid (IL) [Bmpyrr][TFO] (1-butyl-1-methylpyrrolidinium tri-fluoromethanesulfonate). A full factorial design (FFD) was used in order to study the influence of three variables (temperature, mannose/vinyl myristate ratio and total substrate quantity) on the 24 h- . This design led to a model based on a second order polynomial response function. The resulting predicted con-tour plots have shown that the highest 24 h- should be obtained with high temperatures, low sugar/vinylester molar ratio and intermediate total substrate quantities (mmol). The model has been successfully verified and experimentally confirmed at the optimal conditions of 80◦C, substrate molar ratio of 1/10and total substrate quantity of 0.26 mmol leading to the highest predicted 24 h- of 72.2%. [less ▲]

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See detailStudy of the Influence of Pure Ionic Liquids on the Lipase-catalyzed (Trans)esterification of Mannose Based on their Anion and Cation Nature
Galonde, Nadine ULg; Nott, Katherine ULg; Richard, Gaetan ULg et al

in Current Organic Chemistry (2013), 17(7), 763-770

A screening of nine ionic liquids (ILs) has been carried out in order to study the influence of the anion's and cation's nature and structure on the synthesis of mannosyl myristate by (trans ... [more ▼]

A screening of nine ionic liquids (ILs) has been carried out in order to study the influence of the anion's and cation's nature and structure on the synthesis of mannosyl myristate by (trans)esterification catalyzed by Novozym® 435. The best ILs in terms of yield (η) and initial rate (v0) are those based on the TFO- anion. The 24h yield (24h-η) reached 64.9% in [Bmim][TFO] and 70.9% in [Bmpyrr][TFO] by transesterification while it reached 29.7% and 44.5% respectively in each IL by esterification. [Bmpyrr][TFO] based on the pyrrolidinium cation gave the best results although this cation has been rarely used for biocatalysis. This work has thus highlighted a cation that could be further studied in biocatalysis of glycosylated compounds. The study of the relationship between the structure of the ILs and the v0 and η for the biocatalysis of mannosyl myristate showed that the lipase effectiveness is influenced by the anions while the cations have an indirect influence on the interaction strength between Novozym® 435 and the anions. [less ▲]

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See detailReusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids
Galonde, Nadine ULg; Richard, Gaetan ULg; Deleu, Magali ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2013)

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See detailUse of ionic liquids for biocatalytic synthesis of sugar derivatives
Galonde, Nadine ULg; Nott, Katherine ULg; Debuigne, Antoine ULg et al

in Journal of Chemical Technology & Biotechnology (2012), 87(4), 451-471

Sugar-based compounds are widely used in pharmaceuticals, cosmetics, detergents and food. They are mainly produced by chemical methods, but the use of enzymes as ‘a greener alternative’ to organic ... [more ▼]

Sugar-based compounds are widely used in pharmaceuticals, cosmetics, detergents and food. They are mainly produced by chemical methods, but the use of enzymes as ‘a greener alternative’ to organic synthesis has been investigated for more than 20 years. Due to the low polar substrate solubility in organic solvents compatible with enzymes, research has focused on the application of substitutes for biocatalysis, especially ionic liquids (ILs). After introducing the main properties of ILs and especially their ability to solubilize sugars, this review focuses on one of their applications, the biocatalytic synthesis of carbohydrate derivatives. In this context, they can be used in pure IL systems, in IL/IL systems or in IL/organic solvent systems. Finally, this review provides an update on the environmental fate of ILs. Their exploitation in ‘green’ processes is still limited due to their low degradability but research is currently under way to design new more ‘eco-friendly’ ILs. [less ▲]

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See detailUse of pure ionic liquids as solvents for the lipase catalyzed synthesis of fatty acid sugar esters
Galonde, Nadine ULg

Poster (2010, December 10)

Sugar fatty acid esters are non-ionic surfactants commonly used in the pharmaceutical, cosmetic and food industries. Their enzymatic synthesis can be hindered by the low solubility of sugars in the ... [more ▼]

Sugar fatty acid esters are non-ionic surfactants commonly used in the pharmaceutical, cosmetic and food industries. Their enzymatic synthesis can be hindered by the low solubility of sugars in the organic solvents commonly used with lipases. Ionic liquids (ILs) have gained attention as particularly attractive “green” alternatives to organic solvents for carbohydrate biotransformation. Indeed, many ILs are good solvents for polar (e.g. carbohydrates) and less polar compounds. Furthermore, their use as reaction media can enhance the enzymatic reactivity, selectivity and stability. In this context, our study focused on the influence of using ILs as reaction medium for the lipase catalyzed synthesis of mannosyl myristate. The effect of the IL nature on the enzymatic reaction initial yield and rate was examined. [less ▲]

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See detailLipase synthesis of fatty acid sugar esters in pure ionic liquids
Galonde, Nadine ULg

Poster (2010, October 14)

Sugar fatty acid esters are broadly consumed in many commercial applications and their synthesis gained a considerable interest since they are biodegradable, tasteless, odorless, non-toxic and non ... [more ▼]

Sugar fatty acid esters are broadly consumed in many commercial applications and their synthesis gained a considerable interest since they are biodegradable, tasteless, odorless, non-toxic and non-irritant. Ionic liquids are organic salts entirely comprised of ions with a melting point below 100°C. This new category of solvent may allow the enzymatic synthesis of sugar fatty acid ester to go toward a “greener” process. Within the purpose of using “green” systems for synthesis of glycosylated compounds, studies on the use of ionic liquids to obtain fatty acid sugar esters with carbohydrates coming from lignocellulosic material as substrates was carried out. The enzymatic synthesis of mannose myristate ionic liquids as enzymatic medium and the yield obtained will be presented in our poster. [less ▲]

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See detailLes enzymes et leur utilisation en biotechnologie industrielle
Galonde, Nadine ULg

Poster (2010, May 22)

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