References of "GOTHOT, André"
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See detailAre neural crest stem cells the missing link between hematopoietic and neurogenic niches?
Coste, Cécile ULg; Neirinckx, Virginie ULg; Gothot, André ULg et al

in Frontiers in Cellular Neuroscience (in press)

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See detailImmune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation following Flu-TBI versus TLI-ATG Conditioning
HANNON, Muriel ULg; BEGUIN, Yves ULg; Ehx, Grégory ULg et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (in press)

Purpose: A conditioning regimen for allogeneic hematopoietic cell transplantation (HCT) combining total lymphoid irradiation (TLI) plus anti-thymocyte globulin (ATG) has been develop to induce graft ... [more ▼]

Purpose: A conditioning regimen for allogeneic hematopoietic cell transplantation (HCT) combining total lymphoid irradiation (TLI) plus anti-thymocyte globulin (ATG) has been develop to induce graft-versus-tumor effects without graft-versus-host disease (GVHD). Experimental Design: We compared immune recovery in 53 patients included in a phase II randomized study comparing nonmyeloablative HCT following either fludarabine plus 2 Gy total body irradiation (TBI arm, n=28) or 8 Gy TLI plus anti-thymocyte globulin (TLI arm, n=25). Results: In comparison to TBI patients, TLI patients had a similarly low 6-month incidence of grade II-IV acute GVHD, a lower incidence of moderate/severe chronic GVHD (P=0.02), a higher incidence of CMV reactivation (P<0.001), and a higher incidence of relapse (P=0.01). While recovery of total CD8+ T cells was similar in the two groups, with median CD8+ T cell counts reaching the normal values 40-60 days after allo-HCT, TLI patients had lower percentages of naïve CD8 T cells. Median CD4+ T cell counts did not reach the lower limit of normal values the first year after allo-HCT in the two groups. Further, CD4+ T cell counts were significantly lower in TLI than in TBI patients the first 6 months after transplantation. Interestingly, while median absolute regulatory T cell (Treg) counts were comparable in TBI and TLI patients, Treg/naïve CD4+ T cell ratios were significantly higher in TLI than in TBI patients the 2 first years after transplantation. Conclusions: Immune recovery differs substantially between these two conditioning regimens possibly explaining the different clinical outcomes observed (NCT00603954). [less ▲]

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See detailOne injection of platelet-rich plasma associated to a submaximal eccentric protocol to treat chronic jumper's knee
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Bruyère, Olivier ULg et al

in The Journal of sports medicine and physical fitness (in press)

INTRODUCTION: Jumper's knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons ... [more ▼]

INTRODUCTION: Jumper's knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons. METHODS: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments were made before infiltration of PRP, and 6 weeks and 3 months after the infiltration, using a 10--point Visual Analogic Scale, clinical examinations with a pressure algometer, algofunctional scores (IKDC and VISA--P), functional assessments (isokinetic and optojump evaluations) and imagery (ultrasounds and MRI). The PRP was obtained with an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a standardised sub--maximal eccentric reeducation. RESULTS: Pain during daily activities significantly decreased with time. During functional evaluation, it decreased as well, but without significant functional improvement. No improvements in the imagery measurements were observed. Younger patients seemed to be more susceptible to have an improvement of pain by the PRP infiltration. CONCLUSION: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol can improve symptoms of chronic jumper's knee in patients non--responsive to classical conservative treatments. [less ▲]

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See detailUsing platelet-rich plasma to treat jumper's knees: Exploring the effect of a second closely-timed infiltration
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Forthomme, Bénédicte ULg et al

in Journal of Science and Medicine in Sport (in press)

Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of proximal patellar tendinopathy. Although it is possible that a single infiltrative administration may prove ... [more ▼]

Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of proximal patellar tendinopathy. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of two or three successive infiltrations. The aim of this study was to evaluate whether two infiltrations of PRP proves more effective than a single treatment. DESIGN: Prospective, randomized and comparative study of level 2. METHODS: Twenty patients suffering from chronic proximal patellar tendinopathy were enrolled into the study and split into two randomized groups (one or two infiltrations of PRP, respectively). The 3-month follow-up evaluation consisted of VAS, IKDC and VISA-P scores, along with algometer, isokinetic and ultrasounds evaluations. After 1 year, subjects were contacted to define their functional evolution. RESULTS: The concentration of the PRP used for each infiltration was similar in both groups, and contained no red or white cells. Results revealed no difference in treatment efficacy between the groups. CONCLUSIONS: The comparison between one or two infiltrations of PRP did not reveal any difference between the two groups at short to mid term. A second closely-timed infiltration of PRP to treat proximal patellar tendinopathies is not necessary to improve the efficacy of this treatment in the short term. [less ▲]

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See detailNo interest for a second closely-timed infiltration of platelet-rich plasma (PRP) to treat jumper’s knees
Kaux, Jean-François ULg; CROISIER, Jean-Louis ULg; Forthomme, Bénédicte ULg et al

in Radmann, A; Hedenborg, S; Tsolakidis, E (Eds.) 20th annual Congress of the EUROPEAN COLLEGE OF SPORT SCIENCE - BOOK OF ABSTRACTS (2015, June)

Introduction: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to ... [more ▼]

Introduction: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of 2 or 3 successive infiltrations. The aim of this study was to evaluate whether 2 infiltrations of PRP proves moreeffective than a single treatment. Methods: Twenty patients suffering from chronic jumper’s knee were enrolled into our prospective, randomized and comparative study of level 2, and split into two randomized groups (1 or 2 infiltrations of PRP, respectively). The 3-month follow-up evaluation consisted of VAS, IKDC and VISA-P scores, along with algometer, isokinetic and ultrasounds evaluations. After 1 year, subjects were contacted to define their functional evolution. Results: The concentration of the PRP used for each infiltration was similar in both groups, and contained no red or white cells. Results revealed no difference in treatment efficacy between the groups.Discussion: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups at short to mid term. A second closely-timed infiltration of PRP to treat jumper’s knees is not necessary to improve the efficacy of this treatment in the short term. References: Kaux JF, Croisier JL, Bruyere O, Rodriguez de la Cruz C, Forthomme B, Brabant G et al. One injection of platelet-rich plasma associated to a submaximal eccentric protocol to treat chronic jumper’s knee. J Sports Med Phys Fitness. 2014 Jun 19. [Epub ahead of print]. Kaux JF, Croisier JL, Forthomme B, Le Goff C, Buhler F, Savanier C et al. Using platelet-rich plasma to treat jumper’s knees: exploring the effect of a second closely-timed infiltration. J Sci Med Sport. Submitted. Kaux JF, Bruyère O, Croisier JL, Forthomme N, Le Goff C, Rohr N et al. One-year follow-up of platelet-rich plasma infiltration to treat chronic upper patellar tendinopathies. Acta Orthop Belg. In press. [less ▲]

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See detailQuelle place pour le test de génération de thrombine au sein du laboratoire de biologie clinique?
LECUT, Christelle ULg; PETERS, Pierre ULg; MASSION, Paul ULg et al

in Annales de Biologie Clinique (2015), 73(2), 137-149

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See detailReflections about the optimisation of the treatment of tendinopathies with PRP
Kaux, Jean-François ULg; Bouvard, Marc; LECUT, Christelle ULg et al

in Muscles, Ligaments and Tendons Journal (2015), 5(1 (eCollection 2015 Jan-Mar)), 1-4

Background: platelet-rich plasma (PRP) infiltration represents a recent therapy for chronic tendinopathies. However, in the literature, this treatment remains controversial. Purpose: we suggest some ideas ... [more ▼]

Background: platelet-rich plasma (PRP) infiltration represents a recent therapy for chronic tendinopathies. However, in the literature, this treatment remains controversial. Purpose: we suggest some ideas for improving this treatment. Methods: these suggestions were based on a review of published studies and our clinical experience. Conclusion: optimizing the technique for PRP collection is paramount. Different risk factors must be corrected before infiltration and chronic tendinopathies must be carefully selected. Finally, post-infiltration rehabilitation remains absolutely critical. Standardization of the use of PRP remains necessary in order to optimize the results. [less ▲]

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See detailPlasma riche en plaquettes et lésions tendineuses
KAUX, Jean-François ULg; Drion, Pierre ULg; Croisier, Jean-Louis ULg et al

in Revue Médicale de Liège (2014), 69(Synthèse 2014), 72-77

Platelets contain growth factors released during their degranulation following activation. These growth factors promote tissue remodeling, wound healing and angiogenesis. Currently, the clinical effect of ... [more ▼]

Platelets contain growth factors released during their degranulation following activation. These growth factors promote tissue remodeling, wound healing and angiogenesis. Currently, the clinical effect of Platelet-Rich Plasma (PRP) is still discussed or even controversial. Our researches have evaluated the effectiveness of PRP on the healing of animal tendons and human suffering from chronic jumper's knee. [less ▲]

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See detailIntérêt d’une seconde infiltration de plasma riche en plaquettes dans le traitement des tendinopathies patellaires ?
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Buhler, Frédéric et al

in 7ème Congrès National SFMES & SFTS 2014 (2014, September)

Introduction : Des études cliniques ont évalué l’effet du plasma riche en plaquettes (PRP) dans le traitement de la tendinopathie patellaire. Même si une seule infiltration peut améliorer les symptômes du ... [more ▼]

Introduction : Des études cliniques ont évalué l’effet du plasma riche en plaquettes (PRP) dans le traitement de la tendinopathie patellaire. Même si une seule infiltration peut améliorer les symptômes du sportif dans cette indication, la majorité des études évaluent les effets de 3 infiltrations successives. Le but de notre étude était donc de comparer l’efficacité d’une et de 2 infiltrations (rapprochées) de PRP dans le cade de tendinopathies patellaires chroniques, rebelles aux traitements conservateurs. Matériel et méthode : Vingt sportifs souffrant de tendinopathie patellaire depuis plus de 3 mois ont été inclus et randomisés en 2 groupes, respectivement 1 ou 2 infiltrations de PRP (15 jours d’intervalle). Le PRP a été obtenu à l’aide d’une machine d’aphérèse permettant d’obtenir une concentration plaquettaire identique pour tous les sujets. Ils ont bénéficié ensuite d’une rééducation excentrique sous-maximale standardisée. Le suivi a été réalisé à l’aide d’une échelle visuelle analogique de la douleur, de scores algo-fonctionnels (IKDC et VISA-P), ainsi qu’un questionnaire « mode de vie » avant l’infiltration, 3 et 12 mois post-infiltration. Résultats: La concentration de PRP employée était la même au sein des 2 groupes (9x106/µL), et ne contenait pas d’érythrocyte ni de leucocyte. Au terme du suivi (1 an), les résultats montrent une amélioration des scores EVA dans les deux groupes et significative pour le groupe 1 (p=0,005). Les scores VISA-P et IKDC ont tous deux augmentés, significativement dans les deux groupes. Un sujet par groupe n’a pas repris d’activité physique tandis 67% ont repris le sport au niveau antérieur dans le groupe 1 et 78% dans le groupe 2. Conclusion: La comparaison entre 1 et 2 infiltrations rapprochées de PRP n’a pas permis de mettre en évidence de différence entre les 2 groupes sur un suivi d’un an. Une seconde infiltration rapprochée ne semble donc pas indiquée pour améliorer l’efficacité de ce traitement. Cependant, un suivi à plus long terme serait nécessaire. De même l’évaluation d’une seconde infiltration plus à distance (3 mois) de la première mériterait d’être explorée. [less ▲]

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See detailNeutrophil contribution to spinal cord injury and repair
Neirinckx, Virginie ULg; Coste, Cécile ULg; Franzen, Rachelle ULg et al

in Journal of Neuroinflammation (2014), 11(1),

Spinal cord injuries remain a critical issue in experimental and clinical research nowadays, and it is now well accepted that the immune response and subsequent inflammatory reactions are of significant ... [more ▼]

Spinal cord injuries remain a critical issue in experimental and clinical research nowadays, and it is now well accepted that the immune response and subsequent inflammatory reactions are of significant importance in regulating the damage/repair balance after injury. The role of macrophages in such nervous system lesions now becomes clearer and their contribution in the wound healing process has been largely described in the last few years. Conversely, the contribution of neutrophils has traditionally been considered as detrimental and unfavorable to proper tissue regeneration, even if there are very few studies available on their precise impact in spinal cord lesions. Indeed, recent data show that neutrophils are required for promoting functional recovery after spinal cord trauma. In this review, we gathered recent evidence concerning the role of neutrophils in spinal cord injuries but also in some other neurological diseases, highlighting the need for further understanding the different mechanisms involved in spinal cord injury and repair. [less ▲]

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See detailComprehensive plasma profiling for the characterization of graft-versus-host disease biomarkers
De Bock, Muriel; BEGUIN, Yves ULg; Leprince, Pierre ULg et al

in Talanta (2014), 125

Acute graft-versus-host disease (aGVHD) remains a life-threatening complication of hematopoietic stem cell transplantation (HSCT), limiting its application. To optimize management of aGVHD and reduce ... [more ▼]

Acute graft-versus-host disease (aGVHD) remains a life-threatening complication of hematopoietic stem cell transplantation (HSCT), limiting its application. To optimize management of aGVHD and reduce therapy-related toxicity, early specific markers are needed. The main objective of this study was thus to uncover diagnostic biomarkers comparing plasma protein profiles of patients at the time of acute GVHD diagnosis and of patients undergoing HSCT without aGVHD. Additional analysis of samples taken 15 days before aGVHD diagnosis was also performed to evaluate the potential of the newly discovered biomarkers for early diagnosis. To extract a maximum of information from plasma samples, we used three complementary proteomic approaches, namely 2D-DIGE, SELDI-TOF-MS and 2D-LC-MSE. We identified and confirmed by means of a independent techniques, the differential expression of several proteins indicating significantly increased inflammation response and disturbance in the coagulation cascade. The variation of these proteins was already observed 15 days before GVHD diagnosis, suggesting the potential early detection of the disease before symptoms appearance. Finally, logistic regression analysis determines a composite biomarker panel comprising fibrinogen, fragment of fibrinogen beta chain, SAA, prothrombin fragments, apolipoprotein A1 and hepcidin that optimally discriminated patients with and without GVHD. The area under the receiver operating characteristic curve distinguishing these 2 groups was 0.95. [less ▲]

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See detailPlatelet-rich plasma to treat patellar tendinopathies: a 1 year follow-up.
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Bruyère, Olivier ULg et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 1285

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons ... [more ▼]

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons. Infiltration of Platelet Rich Plasma (PRP) may be considered as a recent therapeutic option for chronic tendinopathies. The aim of the current study is to evaluate the clinical status and the return to sports activities in patients with chronic upper patellar tendinopathies up to 1 year after 1 infiltration of PRP. Methods: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments were made before infiltration of PRP, and 6 weeks and 3 months and 1 year after the infiltration, using a 10-point Visual Analogic Scale and algofunctional scores (IKDC and VISA-P). Moreover, they had to answer an information questionnaire concerning their life and sports activities. The PRP was obtained with an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a standardised sub-maximal eccentric reeducation. Results: Pain during daily activities significantly decreased with time. VAS has significantly dropped, IKDC and VISA-P significantly improved over the follow-up of 1 year. Seventy percents of patients reported a favourable evolution with decrease of pain, 15% did never report any improvement and 15% were treated surgically. Seventy percents returned to sports activities, 64,3% without any pain, and 50% of them recovered the same sport level. Younger patients seemed to be more susceptible to have an improvement of pain by the PRP infiltration. Conclusions: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol can improve, at 1 year, symptoms of chronic jumper’s knee in patients non-responsive to classical conservative treatments. [less ▲]

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See detailNo interest for a second close infiltration of platelet-rich plasma to treat upper patellar tendinopathies
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Buhler, Frédéric et al

in European Journal of Physical and Rehabilitation Medicine (2014, May), 50(Suppl. 1 to No. 3), 21639-002-

Background: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to ... [more ▼]

Background: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of 2 or 3 successive infiltrations. The aim of this study was to evaluate whether 2 infiltrations of PRP proves more effective than a single treatment. Methods: Twenty patients suffering from jumper’s knee for over than 3 months were enrolled into the study and split into two randomized groups (1 or 2 infiltrations of PRP, respectively). The follow-up evaluation consisted of VAS, IKDC and VISA-P scores, along with algometer, isokinetic and ultrasounds evaluations. Results: The concentration of the PRP used for each infiltration was similar in both groups, and contained no red or white cells. Results revealed no difference in treatment efficacy between the groups. Conclusion: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups after a follow-up period of 3 months. A second closely-timed infiltration of PRP to treat jumper’s knees is not necessary to improve the efficacy of this treatment in the short term. [less ▲]

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See detailDevelopment of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.
Bouaziz, Ahlem; Walgraffe, David; Bouillot, Celine et al

in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (2014)

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of ... [more ▼]

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of allergy and the safety and efficacy of immunotherapy. OBJECTIVE: The current study reports the immunological characterization of two recombinant molecules of the HDM allergen Der p 3 as useful tools for diagnosis and immunotherapy. METHODS: Recombinant mature (rDer p 3) and immature (proDer p 3) Der p 3 and their corresponding S196A mutants were produced in Pichia pastoris and purified. The stability, IgE-binding capacity and allergenicity of the different proteins were analyzed and compared with those of the major mite allergen Der p 1 used as a reference. Additionally, the immunogenicity of the different allergens was evaluated in a murine model of Der p 3 sensitization. RESULTS: Compared to the IgE reactivity to recombinant and natural Der p 3 (nDer p 3), the mean IgE binding of patient's sera to rDer p 3-S196A (50 %) was higher. The poorly binding to nDer p 3 or rDer p 3 was due to autolysis of the allergen. Contrary to Der p 3, proDer p 3 displayed very weak IgE reactivity, as measured by sandwich ELISA and competitive inhibition, RBL degranulation and human basophil activation assays. Moreover, proDer p 3 induced a TH 1-biased immune response that prevented an allergic response in mice but retained Der p 3-specific T cell reactivity. CONCLUSION: rDer p 3-S196A should be used for the diagnosis of HDM allergy elicited by Der p 3, and proDer p 3 may represent a hypoallergen of Der p 3. This article is protected by copyright. All rights reserved. [less ▲]

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