A deletion in the bovine FANCI gene compromises fertility by causing fetal death and brachyspina.
Charlier, Carole ; ; Coppieters, Wouter et al
in PLoS ONE (2012), 7(8), 43085
Fertility is one of the most important traits in dairy cattle, and has been steadily declining over the last decades. We herein use state-of-the-art genomic tools, including high-throughput SNP genotyping ... [more ▼]
Fertility is one of the most important traits in dairy cattle, and has been steadily declining over the last decades. We herein use state-of-the-art genomic tools, including high-throughput SNP genotyping and next-generation sequencing, to identify a 3.3 Kb deletion in the FANCI gene causing the brachyspina syndrome (BS), a rare recessive genetic defect in Holstein dairy cattle. We determine that despite the very low incidence of BS (<1/100,000), carrier frequency is as high as 7.4% in the Holstein breed. We demonstrate that this apparent discrepancy is likely due to the fact that a large proportion of homozygous mutant calves die during pregnancy. We postulate that several other embryonic lethals may segregate in livestock and significantly compromise fertility, and propose a genotype-driven screening strategy to detect the corresponding deleterious mutations. [less ▲]Detailed reference viewed: 16 (3 ULg)
a genetic marker test for brachyspina and fertility in cattle
Georges, Michel ; Coppieters, Wouter ; Charlier, Carole et al
Patent (2011)Detailed reference viewed: 26 (0 ULg)
Identification of disease-causing mutations allows for unbiased estimation of their overlooked pleïotropic effects on related quantitative traits.
Charlier, Carole ; Sartelet, Arnaud ; Fasquelle, Corinne et al
Poster (2011, February)Detailed reference viewed: 22 (6 ULg)
LUPA: a European initiative taking advantage of the canine genome architecture for unravelling complex disorders in both human and dogs.
Lequarré, Anne-Sophie ; ; et al
in Veterinary Journal (2011), 189(2), 155-9
The domestic dog offers a unique opportunity to explore the genetic basis of disease, morphology and behaviour. Humans share many diseases with our canine companions, making dogs an ideal model organism ... [more ▼]
The domestic dog offers a unique opportunity to explore the genetic basis of disease, morphology and behaviour. Humans share many diseases with our canine companions, making dogs an ideal model organism for comparative disease genetics. Using newly developed resources, genome-wide association studies in dog breeds are proving to be exceptionally powerful. Towards this aim, veterinarians and geneticists from 12 European countries are collaborating to collect and analyse the DNA from large cohorts of dogs suffering from a range of carefully defined diseases of relevance to human health. This project, named LUPA, has already delivered considerable results. The consortium has collaborated to develop a new high density single nucleotide polymorphism (SNP) array. Mutations for four monogenic diseases have been identified and the information has been utilised to find mutations in human patients. Several complex diseases have been mapped and fine mapping is underway. These findings should ultimately lead to a better understanding of the molecular mechanisms underlying complex diseases in both humans and their best friend. [less ▲]Detailed reference viewed: 44 (5 ULg)
Highly effective SNP-based association mapping and management of recessive defects in livestock.
Charlier, Carole ; Coppieters, Wouter ; Rollin, Frédéric et al
in Nature Genetics (2008), 40(4), 449-54
The widespread use of elite sires by means of artificial insemination in livestock breeding leads to the frequent emergence of recessive genetic defects, which cause significant economic and animal ... [more ▼]
The widespread use of elite sires by means of artificial insemination in livestock breeding leads to the frequent emergence of recessive genetic defects, which cause significant economic and animal welfare concerns. Here we show that the availability of genome-wide, high-density SNP panels, combined with the typical structure of livestock populations, markedly accelerates the positional identification of genes and mutations that cause inherited defects. We report the fine-scale mapping of five recessive disorders in cattle and the molecular basis for three of these: congenital muscular dystony (CMD) types 1 and 2 in Belgian Blue cattle and ichthyosis fetalis in Italian Chianina cattle. Identification of these causative mutations has an immediate translation into breeding practice, allowing marker assisted selection against the defects through avoidance of at-risk matings. [less ▲]Detailed reference viewed: 63 (4 ULg)