References of "Frederich, Michel"
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See detailDevelopment of an HPTLC method for determination of hypoglycin A in aqueous extracts of seedlings and samaras of Acer species.
Habyarimana, Jean Belt Adélite ULg; Baise, Etienne; Douny, Caroline ULg et al

E-print/Working paper (2017)

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo ... [more ▼]

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo, is claimed to be present where AM cases were reported in the US. For unknown reasons, occurrence of this disease has increased. It is important to define environmental key factors that may influence toxicity of samaras from Acer species. In addition, the content of HGA in seedlings needs to be determined since AM outbreaks, during autumn period when the seeds fall but also during spring when seeds are germinating. The present study aims to validate a reliable method using high performance thin layer chromatography for determination and comparison of HGA in samaras and seedlings. The working range of the method was between 20 μg HGA to 408 μg HGA per ml water, corresponding to 12 - 244 mg/kg fresh weight or 40 - 816 mg/kg dry weight, taking into account of an arbitrary average dry matter content of 30%. Instrumental limit of detection and limit of quantification were of 10 µg HGA/ml and 20 µg HGA/ml water, respectively. Instrumental precision was 4% (RSD on 20 repeated measurements) while instrumental accuracy ranged between 86% and 121% of expected value. The HGA recovery of the analytical method estimated from spiked samaras and seedlings samples ranged between 63 and 103%. The method was applied to 9 samples of samaras from Acer pseudoplatanus, A. platanoides and A. campestre and 5 seedlings samples from A. pseudoplatanus. The results confirm detection of HGA in samaras from A. pseudoplatanus and the absence of detection in samaras of other tested species. They also suggest that detected levels of HGA are highly variable. This confirmed the suitability of the method for HGA detection in samaras or seedling. [less ▲]

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See detailInsect fatty acids: A comparison of lipids from three Orthopterans and Tenebrio molitor L. larvae
Paul, Aman ULg; Frederich, Michel ULg; Caparros Megido, Rudy ULg et al

in Journal of Asia-Pacific Entomology (2017), 20(2), 337-340

In order to explore some potential insect sources of food lipids, the lipid compositions of three Orthopterans (Acheta domesticus, Conocephalus discolor and Chorthippus parallelus) were analyzed and ... [more ▼]

In order to explore some potential insect sources of food lipids, the lipid compositions of three Orthopterans (Acheta domesticus, Conocephalus discolor and Chorthippus parallelus) were analyzed and compared with those of Tenebrio molitor larvae. A. domesticus, Co. discolor, Ch. parallelus and T. molitor larvae were found to contain approximately 15%, 13%, 10% and 32% lipids on dry weight, respectively. The lipids from three Orthopterans contain much higher amounts of essential fatty acids than those of T. molitor larvae. The two Orthopterans of the suborder Ensifera i.e., A. domesticus and Co. discolor contain linoleic acid in major quantities, while Ch. parallelus of the suborder Caelifera, contain α-linolenic acid in major quantities. The consumption of linoleic and α-linolenic fatty acid is linked with numerous health promoting effects. The factors that contribute to differences in fatty acid profiles of these insects are being discussed. At last the nutritional parameters including polyunsaturated to saturated and omega 6 to omega 3 fatty acid ratios of these insect lipids are also being discussed to understand the potential role of these lipids in human nutrition. [less ▲]

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See detailPolyphenolic profile and biological activities of the leaves and aerial parts of Echinocystis lobata (Michx.) Torr. et A.Gray (Cucurbitaceae)
Ielciu, Irina-Ioana ULg; Vlase, Laurian; Frederich, Michel ULg et al

in Farmacia (2017), 65(2), 179-183

The aim of the present study consisted in the quantification of the polyphenols in leaves and aerial parts of Echinocystis lobata (Michx.) Torr. et A.Gray and testing its biological potential. Analysis of ... [more ▼]

The aim of the present study consisted in the quantification of the polyphenols in leaves and aerial parts of Echinocystis lobata (Michx.) Torr. et A.Gray and testing its biological potential. Analysis of polyphenols was performed by a HPLC-MS method. Antioxidant activity of the extracts was tested by DPPH, CUPRAC, FRAP, TEAC, EPR and SNPAC assays. Cytotoxic activity was assessed on cancerous and healthy cell lines. Anti-plasmodial tests were performed on two strains of Plasmodium falciparum. Ethanolic extracts of E. lobata contain mainly p-coumaric acid, isoquercitrin, rutin, quercitrin and kaempferol. Biological assays showed a significant antioxidant effect and no cytotoxic and anti-plasmodial activity. These results offer a new perspective on E. lobata, proving it as an important source of antioxidant compounds. [less ▲]

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See detailUnguiculin A and Ptilomycalins E-H, Antimalarial Guanidine Alkaloids from the Marine Sponge Monanchora unguiculata.
Campos, Pierre-Eric; Wolfender, Jean-Luc; Quieroz, Emerson F. et al

in Journal of Natural Products (2017), 80

Chemical study of the CH2Cl2-MeOH (1:1) extract from the sponge Monanchora unguiculata collected in Madagascar highlighted five new compounds, one acyclic guanidine alkaloid, unguiculin A (1) and four ... [more ▼]

Chemical study of the CH2Cl2-MeOH (1:1) extract from the sponge Monanchora unguiculata collected in Madagascar highlighted five new compounds, one acyclic guanidine alkaloid, unguiculin A (1) and four pentacyclic alkaloids, ptilomycalins E-H (2-5), along with four known compounds: crambescidin 800 (6) and crambescidin 359 (7), crambescidic acid (8), and fromiamycalin (9). Their structures were elucidated by 1D and 2D NMR spectra and HRESIMS data. All compounds were evaluated for their cytotoxicity against KB cells and their antiplasmodial activity. The new ptilomycalin E (2) and the mixture of the new ptilomycalins G (4) and H (5) showed promising cytotoxicity against KB cells with IC50 values of 0.85 and 0.92 μM, respectively. Ptilomycalin F (3) and fromiamycalin (9) exhibited promising activity against Plasmodium falciparum with IC50 values of 0.23 and 0.24 μM, respectively [less ▲]

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See detailCyclotheonellazoles A–C, Potent Protease Inhibitors from the Marine Sponge Theonella aff. swinhoei
Issac, Michal; Aknin, Maurice; Gauvin-Bialecki, Anne et al

in Journal of Natural Products (2017), 80

he extract of a sample of the sponge Theonella aff. swinhoei collected in Madagascar exhibited promising in vitro antiplasmodial activity. The antiplasmodial activity was ascribed in part to the known ... [more ▼]

he extract of a sample of the sponge Theonella aff. swinhoei collected in Madagascar exhibited promising in vitro antiplasmodial activity. The antiplasmodial activity was ascribed in part to the known metabolite swinholide A. Further investigation of the extract afforded three unusual cyclic peptides, cyclotheonellazoles A–C (1–3), which contain six nonproteinogenic amino acids out of the eight acid units that compose these natural products. Among these acids the most novel were 4-propenoyl-2-tyrosylthiazole and 3-amino-4-methyl-2-oxohexanoic acid. The structure of the compounds was elucidated by interpretation of the 1D and 2D NMR data, HRESIMS, and advanced Merfay’s techniques. The new compounds were found to be nanomolar inhibitors of chymotrypsin and sub-nanomolar inhibitors of elastase, but did not present antiplasmodial activity. [less ▲]

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See detailAntimalarial Activities of Alkyl Cyclohexenone Derivatives Isolated from the Leaves of Poupartia borbonica
Ledoux, Allison ULg; St-Gelais, Alexis; Cieckiewicz, Ewa ULg et al

in Journal of Natural Products (2017), 80(6), 1750-1757

Bioactivity-guided fractionation of the ethyl acetate extract of the leaves of Poupartia borbonica led to the isolation of three new alkyl cyclohexenone derivatives 1−3, and named Poupartone A−C. The ... [more ▼]

Bioactivity-guided fractionation of the ethyl acetate extract of the leaves of Poupartia borbonica led to the isolation of three new alkyl cyclohexenone derivatives 1−3, and named Poupartone A−C. The structures of the new compounds were elucidated by 1D and 2D NMR spectroscopic data analysis and MS, whereas calculated and experimental ECD spectra were used to define the absolute configurations. These compounds were active against 3D7 and W2 Plasmodium falciparum strains with IC50 values between 0.55 and 1.81 μM. In vitro cytotoxicity against WI38 human fibroblasts and the human cervical cancer cell line HeLa (WST-1 assay) showed that these compounds were also cytotoxic, but no hemolytic activity was observed for the extract and pure compounds. An in vivo antimalarial assay was performed on the major cyclohexenone using P. berghei-infected mice at a dose of 15 mg/kg/day ip. The assay revealed growth inhibition of 59.1 and 69.5% at days 5 and 7 postinfection, respectively, although some toxicity was observed. Zebrafish larvae were used as a model to determine the type of toxicity, and the results showed cardiac toxicity. The methanol extract was also studied, and it displayed moderate antiplasmodial properties in vitro. This extract contained the known flavonoids, quercetin, 3′-O-hydroxysulfonylquercetin, quercitrin, and isoquercitrin as well as ellagic acid, which showed high to low activity against the 3D7 P. falciparum strain. [less ▲]

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See detailAntihypertensive and vasorelaxant effects of aqueous extract of Artemisia campestris L. from Eastern Morocco.
Dib, Ikram; Tits, Monique ULg; Angenot, Luc ULg et al

in Journal of Ethnopharmacology (2017), 206

ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia campestris L. (Asteraceae) has many traditional uses, among which treatment of diabetes and hypertension. AIM OF THE STUDY: This study was conducted in order to ... [more ▼]

ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia campestris L. (Asteraceae) has many traditional uses, among which treatment of diabetes and hypertension. AIM OF THE STUDY: This study was conducted in order to confirm the antihypertensive and hypotensive effects of A. campestris L. aqueous extract (AcAE) and to explore the underlying mechanism of action of its vasorelaxant effect, besides the acute toxicity. Also, the chemical composition of AcAE was investigated. MATERIAL AND METHODS: the chemical content of AcAE was determined by using HPLC and NMR techniques. The antihypertensive effect was assessed indirectly by tail-cuff method on L-NAME induced hypertensive rats, while the hypotensive action was monitored intravenously by invasive method on normotensive rats. The vasorelaxant effect and vascular mechanism of action were studied in the presence of antagonists and blockers on aorta isolated from normotensive rats. On the other side, the acute toxicity was studied by oral feeding of extract to the mice. RESULTS: The global phytochemical profile of AcAE reveals the presence of several polyphenols as main components. A. campestris L. infusion was characterized by mono- and di-cinnamoyl compounds, with 3,5-dicaffeoylquinic (isochlorogenic A) acid being the main compound, followed by 5-caffeoylquinic (chlorogenic) acid. Vicenin-2 (apigenin 6,8-di-C-glucoside) appeared to be the most abundant compound among flavonoids. The daily treatment with AcAE at 150mg/Kg/day prevented the installation of hypertension on L-NAME hypertensive rats, and reduced SBP from 172mmHg up to 144mmHg. At the dose 40mg/Kg, AcAE provoked reduction of systolic (SBP), diastolic (DBP) and mean arterial pressure (MAP), without affecting the heart rate. Also, AcAE (10-2-2mg/ml) relaxed the precontracted aorta by 95.8 +/- 1.3%. The denudation and preincubation of aorta with atropine, calmidazolium, L-NAME, hydroxycobalamin, ODQ, 8-RP-Br-PET-cGMP, thapsigargin and verapamil attenuated the vasorelaxant response, while the pre-treatment with 4-AP, TEA, glibenclamide and BaCl2 did not alter this effect. The oral administration of AcAE (0-6g/Kg) reveals no mortality or toxicity. CONCLUSIONS: our study proved that AcAE possess an important antihypertensive, hypotensive and vasorelaxant effect, which is mediated via calmodulin-NO-cGC-PKG pathway, and via inhibition of calcium influx through voltage-operated calcium channels and activation of intracellular calcium mobilization into sarcoplasmic reticulum. Therefore, our findings give first evidence about the traditional use of A. campestris L. as antihypertensive plant. [less ▲]

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See detailComparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species
Kapepula, Paulin Mutwale; Kabamba Ngombe, Nadege; Tshisekedi Tshibangu, Pascal et al

in Natural Product Research (2017), 6419(March), 1--8

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See detailAntiplasmodial activity of Mezoneuron benthamianum leaves and identification of its active constituents
Jansen, Olivia ULg; Tchinda, Alembert T.; Loua, Jean et al

in Journal of Ethnopharmacology (2017), 203(March), 20--26

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See detailAntiplasmodial activity of Heinsia crinita (Rubiaceae) and identification of new iridoids.
Tshisekedi Tshibangu, P.; Mutwale Kapepula, P.; Kabongo Kapinga, M. J. et al

in Journal of Ethnopharmacology (2017), 196

ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in ... [more ▼]

ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in the Democratic Republic of the Congo (DRC) as a remedy against malaria. Investigations were conducted on crude extracts of leaves, fruits and stem barks in view to validate their use and to determine which plant part possesses the best antiplasmodial properties. MATERIALS AND METHODS: Different plant parts were extracted with methanol, ethanol and dichloromethane. Based on the preliminary assays, the dichloromethane extract of the stem bark was subjected to fractionation using preparative HPLC system and column chromatography. This step led to the isolation of two new iridoids which had their structures elucidated by NMR, UV, MS and FT-IR spectroscopic techniques. Extracts and pure compounds were tested in vitro against the 3D7 strain of Plasmodium falciparum. The inhibition of the parasite growth was evaluated in vitro by colorimetric method (p-LDH assay) and their cytotoxicity evaluated in vitro against the human non-cancer fibroblast cell line (WI38) through WST1 assay. The in vivo antiplasmodial activity was assessed by the inhibition of Plasmodium berghei growth in infected mice treated with the ethanol extract of H. crinita stem bark at the concentrations of 200 and 300mg/Kg/day per os, using a protocol based on the 4-d suppressive test of Peters and compared to a non-treated negative control group of mice (growth =100%). Finally the antioxidant activity of the same extract was evaluated using ABTS, DPPH and cell-based assays. RESULTS: A moderate in vitro antiplasmodial activity was observed for the dichloromethane extract of the stem bark of H. crinita (IC50 =29.2+/-1.39microg/mL) and for the two new iridoids, lamalbide 6, 7, 8- triacetate (IC50 =16.39+/-0.43microg/mL) as well as for its aglycone lamiridosin 6, 7, 8-triacetate (IC50 =0.44.56+/-1.12microg/mL). The ethanolic stem bark extract (200 and 300mg/kg/day, oral route) showed a moderate in vivo antimalarial activity in Plasmodium berghei-infected mice with 27.84+/-2.75% and 48.54+/-3.76% of inhibition of the parasite growth, respectively (p<0.01).). This extract displayed high cellular antioxidant activity using dichlorofluorescein-diacetate (DCFDA) on HL-60 monocytes. These crude extracts and pure compounds tested at the higher concentration of 100microg/mL did not show any cytotoxicity against WI38 cells. CONCLUSIONS: The results showed that H. crinita extracts possess antimalarial activity and contain some unusual iridoids with moderate antiplasmodial activity, therefore justifying to some extent its traditional use by the local population in DRC for this purpose. This is the first report of the isolation and antiplasmodial activity of these two new iridoids. [less ▲]

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See detailMetabolomic and molecular signatures of Mascarene Aloes using a multidisciplinary approach
Govinden-Soulange, J.; Lobine, D.; Frederich, Michel ULg et al

in South African Journal of Botany (2017), 108

In this research a multidisciplinary approach was used to unveil the genetic, metabolic uniqueness and relationships of endemic Mascarene Aloes (Aloe macra, Aloe purpurea, Aloe tormentorii) with respect ... [more ▼]

In this research a multidisciplinary approach was used to unveil the genetic, metabolic uniqueness and relationships of endemic Mascarene Aloes (Aloe macra, Aloe purpurea, Aloe tormentorii) with respect to Aloe vera. Nuclear magnetic resonance spectroscopy, DNA sequencing and antioxidant profiles of these Aloes were studied. Principal component analysis following 1H NMR revealed the specificity of the Mascarene Aloes relative to Aloe vera. The superior free radical scavenging ability of A. purpurea, A. macra and A. tormentorii as compared to other Aloes was also unveiled. Phylogenetic analyses of chloroplast genes and ITS region sequences of these Mascarene Aloes were done using maximum parsimony and Bayesian analysis. Mascarene Aloes clustered within one clade separate from Aloe vera confirming their relative recent emergence in this genus. Results from this study showed that there is sufficient evidence at the metabolomic and molecular level to distinguish between Aloe ​purpurea from Mauritius and that of Reunion. © 2016 SAAB [less ▲]

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See detailANTICANCER, ANTIPLASMODIAL AND ANTITRYPANOSOMAL ACTIVITIES OF CRUDE EXTRACTS OF PLATANUS ORIENTALIS
Ebralidze, L.; Mskhiladze, Lasha; Ledoux, Allison ULg et al

in World Journal of Pharmaceutical Research (2017), 6(3), 170-175

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See detailQualité des médicaments antipaludéens et caractéristiques des pharmacies des territoires périurbains de Kinshasa
Mavungu Landu, Don Jethro ULg; Liégeois, Sophie; Manzambi Kuwekita, Joseph ULg et al

Poster (2016, December 14)

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le ... [more ▼]

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le contrôle de cette maladie. Cependant en Afrique centrale, plus de 25% des médicaments serait contrefaits ou de qualité inférieure, situation qui serait encore plus dramatique dans les territoires périurbains. Méthode: Dans le contexte ci-mentionné, une étude préliminaire et prospective a été menée dans la zone de santé de Mont Ngafula 1 située dans les territoires périurbains de la Ville de Kinshasa durant la période allant du 22 février au 17 mars 2016. Treize échantillons de poudre pour suspension d’artéméther et de luméfantrine ont été collectés. L’analyse de la qualité de ces médicaments a été réalisée au moyen de méthodes séparatives génériques utilisant la technique de chromatographie liquide à haute performance couplé à un détecteur à barrettes de diodes. Une caractérisation a été également effectuée dans 127 établissements pharmaceutiques sur base des normes édictées par le Ministère de la Santé Publique congolais. Résultats: Les résultats des analyses des échantillons d’antimalariques (ou antipaludéens) montre que presque la moitié des poudres pour suspension d’artéméther et de luméfantrine ne contenait pas la concentration prévue en artéméther et/ou en luméfantrine. Par ailleurs, le résultat des observations évoque qu’aucun établissement pharmaceutique ne respecte l’ensemble des normes du Ministère de la Santé Publique. [less ▲]

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See detailNutritional Composition and Rearing Potential of the Meadow Grasshopper (Chorthippus parallelus Zetterstedt)
Paul, Aman ULg; Frederich, Michel ULg; Uyttenbroeck, Roel ULg et al

in Journal of Asia-Pacific Entomology (2016), 19(4), 1111-1116

Insects, particularly those belonging to the family Acrididae (grasshoppers), are commonly consumed as human food in many parts of the world. Grasshoppers of the species Chorthippus parallelus are ... [more ▼]

Insects, particularly those belonging to the family Acrididae (grasshoppers), are commonly consumed as human food in many parts of the world. Grasshoppers of the species Chorthippus parallelus are abundantly found throughout Europe. However, these insects were not consumed by Europeans till now, but could possibly be used as human food, which is why we investigated their chemical composition. We found that they contain high level of proteins (69%), with an excellent amino acid profile and protein digestibility (97%). Furthermore, specimens of C. parallelus have an interesting fatty acids profile and minerals composition. Preliminary toxicity assessment indicates that these insects do not exhibit toxicity towards neutrophil cells (white blood cells). These data suggest that C. parallelus could be considered for human consumption. Rearing trials done during the study show that commercial rearing could be developed to produce sufficient biomass for sustaining human consumption. [less ▲]

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See detailPotential Antimalarials from Tropical Plants and Natural Products
Frederich, Michel ULg; Ledoux, Allison ULg

Scientific conference (2016, November 16)

Malaria is caused by Plasmodium, a protozoan parasite transmitted by anopheles mosquitoes, and was responsible for 438 000 deaths worldwide in 2015 according to the last World Malaria report.1 The ... [more ▼]

Malaria is caused by Plasmodium, a protozoan parasite transmitted by anopheles mosquitoes, and was responsible for 438 000 deaths worldwide in 2015 according to the last World Malaria report.1 The resistance of parasites to available and affordable medicines has become a widespread problem in exposed countries, making the search of new antimalarial compounds still necessary. As the immeasurable therapeutic potential of plants is well established2, natural products could be an interesting source of new antimalarial drugs. Indeed, according Newmann and Cragg, more than 60% of the antiparasitic drugs discovered between 1981 and 2014 were unaltered naturals products (12.5%), natural products derivatives (31.3%) and synthetic drugs with a natural product pharmacophore (18.8%).3 Then, the pharmacological and phytochemical study of plants, especially from traditional pharmacopoeias can be of first interest to discovered new antimalarial compound and also to valorize the traditional knowledge. The first part of the talk will concern some examples of results obtained in the lab : the in vivo activity of strychnogucine B, which was isolated from strychnos icaja and semi-synthetized, the optimization of ellagic acid as a lead in the treatment of malaria and the validation of MIT from Mezoneuron benthamianum. In the second part of the talk, the work about Poupartia borbonica, an endemic plant from Reunion Island studied in the framework of a collaboration with the University of Reunion Island will be presented. [less ▲]

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See detailValorization of Seeds from Some Field Border Flowering Seeds
Paul, Aman ULg; Danthine, Sabine ULg; Mutwale Kapepula, Paulin ULg et al

Poster (2016, September 20)

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It ... [more ▼]

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It could be really interesting to valorize some commonly grown plant in these strips to render food or health promoting compounds. With this objective in mind the seeds of Achillea millefolium, Anthriscus sylvestris and Prunella vulgaris were investigated for lipids, proteins and phenolic content. Further the lipids were analyzed for fatty acid profile using gas chromatography and the phenolic compounds in the methanolic extract of defatted seeds were identified using HPLC-DAD. The antiradical activity of the methanolic extracts obtained from defatted seeds was investigated using DPPH and ABTS assays. The anti-inflammatory potential of these seed extracts was evaluated on the reactive oxygen species (ROS) production by stimulated neutrophils and on the specific activity of myeloperoxidase (MPO), a pro-oxidant enzyme marker of inflammation. Seeds from all three plants were analyzed with interesting levels of lipids, proteins and phenolic content. Linoleic acid, oleic acid and alpha-linolenic acid were the major fatty acids analyzed in A. millefolium, A. sylvestris and P. vulgaris respectively. On the other hand different phenolic acid formed the major phenolic constituents. Seed extracts displayed high ABTS and DPPH radical-scavenging activities in a dose dependent manner. Also a strong dose dependent anti-inflammatory activity of all three extracts was observed against ROS production by neutrophils and MPO activity. Results indicate that these seed show a great potential to render lipids which could be utilized as human food, further the defatted seeds could be directly included in human diet due to interesting levels of proteins and anti-inflammation ability. [less ▲]

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See detailNew antimalarial compounds isolated from Poupartia borbonica, a Mascarene Islands endemic plant
Ledoux, Allison ULg; Jansen, Olivia ULg; St-Gelais, Alexis et al

in Planta Medica (2016, July), 82(S01), 1-381

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See detailStudy of Mezoneuron benthamianum, a plant traditionally used against malaria in Guinea
Tchinda Tiabou, Alembert ULg; Loua, Jean; Esters, Virginie ULg et al

Poster (2016, July)

Despite some improvements in malaria control, this parasitic disease remains a major public health problem in many African countries, causing about 400 000 deaths/year through the continent, mainly by ... [more ▼]

Despite some improvements in malaria control, this parasitic disease remains a major public health problem in many African countries, causing about 400 000 deaths/year through the continent, mainly by children under the age of five (WHO, 2015). In Guinea, the leaves of Mezoneuron benthamianum Baill. are traditionally used to treat malaria (Traore et al., 2013) and showed a good antiplasmodial activity in an antiprotozoal in vitro screening (Traore et al., 2014), as well as promising results in a preliminary small-scale ethnomedical study (unpublished data), encouraging us to continue the study of this plant. The aim of this work was to evaluate the activity of M. benthamianum leaves extracts against P. falciparum using an in vitro test model (p-LDH assay) and to undertake a bio-guided fractionation to identify the compounds responsible for the activity. Hydroethanolic extracts (70% v/v) of M. benthamianum leaves showed a moderate in vitro activity against P. falciparum 3D7, with IC50 = 22.5 – 32.6 µg/ml, depending on the batch; while a dark precipitate formed during ethanol evaporation showed higher activity (IC50 = 6,5µg/ml). The bioguided fractionation was performed on this most active fraction and allowed the isolation of three diterpens, two flavonoids, resveratrol, gallic acid and its ethylester, β-sitosterol glucoside and pheophorbide derivatives. The active compounds belong to several phytochemical classes, including flavonoids, pheophorbide and gallic acid derivatives, contributing together to the global antiplasmodial activity of the hydroalcoholic extract against P. falciparum parasite. This study gives some concrete evidence to support the ethnopharmacological use of Mezoneuron benthamianum leaves extract in the management of malaria. The active compounds can be further studied for their antiplasmodial potential, as well as their suitability to be used as quality markers for the standardization of this herbal drug from the Guinean traditional pharmacopeia. [less ▲]

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