References of "Franck, Fabrice"
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See detailPSI Mehler reaction is the main alternative photosynthetic electron pathway in Symbiodinium sp., symbiotic dinoflagellates of cnidarians
Roberty, Stéphane ULg; Bailleul, Benjamin ULg; Berne, Nicolas et al

in New Phytologist (2014), 204(1), 81-91

• Photosynthetic organisms have evolved various photoprotective and regulatory mechanisms to cope with changing and high light intensities. The nature and relative amplitude of these mechanisms is matter ... [more ▼]

• Photosynthetic organisms have evolved various photoprotective and regulatory mechanisms to cope with changing and high light intensities. The nature and relative amplitude of these mechanisms is matter of debate in dinoflagellates that live in symbiosis with cnidarians. In this work, the amplitude of photosynthetic alternative electron flows to oxygen (chlororespiration, Mehler reaction, mitochondrial respiration) and PSI cyclic electron flow were investigated in Symbiodinium clades A1, B1 and F1. • Cultured Symbiodinium strains were maintained semi-continuously under identical environmental conditions. Joint measurements of oxygen evolution, fluorescence emission, and absorption changes at specific wavelengths were used to evaluate PSI and PSII electron transfer rates (ETR). • A light-dependent and O2-dependent ETR was observed in all strains. This ETR involved PSII and PSI and was insensitive to addition of mitochondrial and carbon fixation inhibitors. • These results demonstrate that photoreduction of oxygen downstream of PSI by the so-called Mehler reaction is the main alternative electron sink at the onset and steady state of photosynthesis in all strains. This mechanism in Symbiodinium sustains significant levels of photosynthetic electron flux under high light, thus acting as a photoprotective mechanism and increasing the ATP/NADPH ratio by the electron transfer chain. [less ▲]

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See detailLiver proteomic response to hypertriglyceridemia in human-apolipoprotein C-III transgenic mice at cellular and mitochondrial compartment levels
Ehx, Grégory ULg; Gerin, Stéphanie ULg; Mathy, Grégory et al

in Lipids in Health and Disease (2014), 13

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute ... [more ▼]

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute pancreatitis. The present study was undertaken to investigate the impact of hypertriglyceridemia on the mitochondrial, sub-mitochondrial and cellular proteomes in the hepatocytes of a hypertriglyceridemic transgenic mouse model overexpressing the human apolipoproteinC-III. Methods: Quantitative comparative proteomics (2D-DIGE) was carried out in both “low-expressor” (LE) and “high-expressor” (HE) mice, respectively exhibiting moderate and severe HTG, to characterize the effect of the TG plasma level on the proteomic response. Results: The mitoproteome analysis revealed the occurrence of a large-scale adaptation in transgenic mice consisting of a general down-regulation of matricial proteins and up-regulation of inner membrane proteins. Remarkably, the magnitude of these proteomic changes appears to strongly depend on the TG plasma level. Altogether, our different analyses indicate that, in HE mice, the capacity of several metabolic pathways is altered to promote the availability of acetyl-CoA, glycerol-3-phosphate, ATP and NADPH for de novo TG biosynthesis. The up-regulation of several cytosolic ROS detoxifying enzymes also tend to confirm that the cytoplasm of HTG mice is subjected to oxidative stress as previously stated. The up-regulation of cytosolic ferritin indicates that iron over-accumulation could take place in the cytosol of HE mice hepatocytes and contribute to (i) enhance oxidative stress and (ii) promote cellular proliferation. Conclusions: The present analyses demonstrate that important TG dose-responsive metabolic adaptations are set up in human apolipoproteinC-III-overexpressing mice. Our results indicate that these adaptations could support the higher TG production rates which have been previously reported in this HTG model, and also suggest that cytosolic oxidative stress may result from FFA over-accumulation, iron overload and enhanced activity of some ROS-producing catabolic enzymes. [less ▲]

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See detailApplications des microalgues
Franck, Fabrice ULg; Remacle, Claire ULg

Conference (2014, May 22)

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See detailCumulative effect of Aox1 inactivation and N source modulation on the cellular proteome of Chlamydomonas reinhardtii - New insights in the metabolic interconnexion between respiration and photosynthesis
Gerin, Stéphanie ULg; Mathy, Grégory; Franck, Fabrice ULg

Conference (2014, April 14)

In Chlamydomonas reinhardtii, the Aox1 gene encoding the mitochondrial alternative oxidase (AOX) is tightly regulated by the N source: its transcription is stimulated by nitrate and repressed by ammonium ... [more ▼]

In Chlamydomonas reinhardtii, the Aox1 gene encoding the mitochondrial alternative oxidase (AOX) is tightly regulated by the N source: its transcription is stimulated by nitrate and repressed by ammonium. AOX has been proposed to play a key role in regards to the N source by acting as a trigger factor for the TCA cycle to promote the production of reduced cofactors which, together with photo-generated reductant, could contribute to support nitrate assimilation. In the present work, the cellular proteomes of wild-type and AOX-deficient strains grown either on nitrate or ammonium in the presence of acetate have been compared using two dimensional-differential in-gel electrophoresis (2D-DIGE) coupled to hierarchical clustering and ANOVA-2 statistical analyses in order to further investigate the physiological role(s) of AOX, particularly in regards to the N source. The dramatic up-regulation of chloroplastic components of the carbon-concentrating mechanism (CCM) in response to Aox1 inactivation importantly suggests that AOX plays a key role in the CCM in both N sources by allowing the production of CO2 through the TCA cycle. The concomitant down-regulation of photorespiratory enzymes in cells lacking AOX indicates that such an induction of the CCM could actually be sufficient to over-compensate the effects of AOX deficiency on CO2 availability in the local environment of Rubisco and maintain high rates of inorganic carbon fixation despite lower TCA cycle-mediated CO2 production. The global overexpression of diverse photosynthetic chain and Calvin cycle components in cells lacking AOX further evidences that a tight functional relationship exists between mitochondrial and chloroplastic processes of energy transduction, and that there could be a global redirection of bioenergetics towards photosynthesis to limit the harmful consequences (particularly oxidative stress) of mitochondrial deficiency. The observation that numerous N-responsive proteomic modifications are strongly attenuated (or even abolished) in response to Aox1 inactivation supports that AOX also exerts a more specific role as a central effector of the metabolic plasticity induced by modulating the N source in the growth medium. Our proteomic results tend notably to confirm that respiration (through AOX activity) and photosynthesis collaborate to provide nitrate reduction with electrons in mixotrophic condition and that acetate oxidation could be promoted to support AOX involvement in this context. [less ▲]

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See detailStudy of an undershoot in chlorophyll fluorescence signal after a saturating pulse in PAM measurements
Fratamico, Anthony ULg; Cardol, Pierre ULg; Tocquin, Pierre ULg et al

Conference (2014, April 14)

In 1989, Larcher and Neuner have reported the observation of a sudden reversible drop in modulated chlorophyll fluorescence measurements (PAM) immediately after a saturating pulse, and called it “low ... [more ▼]

In 1989, Larcher and Neuner have reported the observation of a sudden reversible drop in modulated chlorophyll fluorescence measurements (PAM) immediately after a saturating pulse, and called it “low-wave”. 25 years later, whereas some papers reported this phenomenon as a trivial detail, scarcely two works have investigated the origin of this particular signal, in which a link with a low CO2 availability seems clear. Our work on Haematococcus pluvialis, a freshwater green microalga, provides a new point of view on this fluorescence undershoot, caused by a rapidly established non-photochemical quenching. We have demonstrated that in the light-adapted state, a low-wave after a saturating flash can be considered as a consequence of an induction process engaged in response to the brief light increase under low CO2 conditions. The non-photochemical quenching during low-waves was found to be dependent on electron transport to oxygen during the preceding flash. Moreover, in conditions in which low-waves were observed in the light-adapted state, the fluorescence induction kinetics in the first minute of lighting after dark-adaptation presented also a reversible strong drop. Nevertheless, at the stationary state, oxygen production and photochemical yield of photosystem II were not affected. Providing a better understanding of the processes underlying low-waves, our work also draws attention on the effect of CO2 concentration on the onset of photosynthesis. [less ▲]

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See detailAnalysis of PSII antenna size heterogeneity of Chlamydomonas reinhardtii during state transitions
de Marchin, Thomas ULg; Ghysels, Bart ULg; Nicolay, Samuel ULg et al

in Biochimica et Biophysica Acta-Bioenergetics (2014), 1837(1), 121-130

PSII antenna size heterogeneity has been intensively studied in the past. Based on DCMU fluorescence rise kinetics, multiple types of photosystems with different properties were described. However, due to ... [more ▼]

PSII antenna size heterogeneity has been intensively studied in the past. Based on DCMU fluorescence rise kinetics, multiple types of photosystems with different properties were described. However, due to the complexity of fluorescence signal analysis, multiple questions remain unanswered. The number of different types of PSII is still debated as well as their degree of connectivity. In Chlamydomonas reinhardtii we found that PSIIα possesses a high degree of connectivity and an antenna 2-3 times larger than PSIIβ, as described previously. We also found some connectivity for PSIIβ in contrast with the majority of previous studies. This is in agreement with biochemical studies which describe PSII mega-, super- and core- complexes in Chlamydomonas. In these studies, the smallest unit of PSII in vivo would be a dimer of two core complexes hence allowing connectivity. We discuss the possible relationships between PSIIα and PSIIβ and the PSII mega-, super- and core- complexes. We also showed that strain and medium dependent variations in the half-time of the fluorescence rise can be explained by variations in the proportions of PSIIα and PSIIβ. When analyzing the state transition process in vivo, we found that this process induces an inter-conversion of PSIIα and PSIIβ. During a transition from state 2 to state 1, DCMU fluorescence rise kinetics are satisfactorily fitted by considering two PSII populations with constant kinetic parameters. We discuss our findings about PSII heterogeneity during state transitions in relation with recent results on the remodeling of the pigment-protein PSII architecture during this process. [less ▲]

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See detailModeling the dependence of respiration and photosynthesis upon light, acetate, carbon dioxide, nitrate and ammonium in Chlamydomonas reinhardtii using design of experiments and multiple regression
Gerin, Stéphanie ULg; Mathy, Gregory; Franck, Fabrice ULg

in BMC Systems Biology (2014), 8(96),

Background: In photosynthetic organisms, the influence of light, carbon and inorganic nitrogen sources on the cellular bioenergetics has extensively been studied independently, but little information is ... [more ▼]

Background: In photosynthetic organisms, the influence of light, carbon and inorganic nitrogen sources on the cellular bioenergetics has extensively been studied independently, but little information is available on the cumulative effects of these factors. Here, sequential statistical analyses based on design of experiments (DOE) coupled to standard least squares multiple regression have been undertaken to model the dependence of respiratory and photosynthetic responses (assessed by oxymetric and chlorophyll fluorescence measurements) upon the concomitant modulation of light intensity as well as acetate, CO2, nitrate and ammonium concentrations in the culture medium of Chlamydomonas reinhardtii. The main goals of these analyses were to explain response variability (i.e. bioenergetic plasticity) and to characterize quantitatively the influence of the major explanatory factor(s). Results: For each response, 2 successive rounds of multiple regression coupled to one-way ANOVA F-tests have been undertaken to select the major explanatory factor(s) (1st-round) and mathematically simulate their influence (2nd-round). These analyses reveal that a maximal number of 3 environmental factors over 5 is sufficient to explain most of the response variability, and interestingly highlight quadratic effects and second-order interactions in some cases. In parallel, the predictive ability of the 2nd-round models has also been investigated by k-fold cross-validation and experimental validation tests on new random combinations of factors. These validation procedures tend to indicate that the 2nd-round models can also be used to predict the responses with an inherent deviation quantified by the analytical error of the models. Conclusions: Altogether, the results of the 2 rounds of modeling provide an overview of the bioenergetic adaptations of C. reinhardtii to changing environmental conditions and point out promising tracks for future in-depth investigations of the molecular mechanisms underlying the present observations. [less ▲]

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See detailLack of isocitrate lyase in Chlamydomonas leads to changes in carbon metabolism and in the response to oxidative stress under mixotrophic growth.
Plancke, Charlotte; Vigeolas, Hélène ULg; Hohner, Ricarda et al

in The Plant journal : for cell and molecular biology (2014), 77(3), 404-417

Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the ... [more ▼]

Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the tricarboxylic acid (TCA) cycle in which CO2 is evolved. In this paper, a null icl mutant of the green microalga Chlamydomonas reinhardtii is described. Our data show that isocitrate lyase is required for growth in darkness on acetate (heterotrophic conditions), as well as for efficient growth in the light when acetate is supplied (mixotrophic conditions). Under these latter conditions, reduced acetate assimilation and concomitant reduced respiration occur, and biomass composition analysis reveals an increase in total fatty acid content, including neutral lipids and free fatty acids. Quantitative proteomic analysis by 14 N/15 N labelling was performed, and more than 1600 proteins were identified. These analyses reveal a strong decrease in the amounts of enzymes of the glyoxylate cycle and gluconeogenesis in parallel with a shift of the TCA cycle towards amino acid synthesis, accompanied by an increase in free amino acids. The decrease of the glyoxylate cycle and gluconeogenesis, as well as the decrease in enzymes involved in beta-oxidation of fatty acids in the icl mutant are probably major factors that contribute to remodelling of lipids in the icl mutant. These modifications are probably responsible for the elevation of the response to oxidative stress, with significantly augmented levels and activities of superoxide dismutase and ascorbate peroxidase, and increased resistance to paraquat. [less ▲]

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See detailStudy of photosynthesis of Chlamydomonas reinhardtii under High and Low CO2 conditions.
de Marchin, Thomas ULg; Ghysels, Bart ULg; Franck, Fabrice ULg

Poster (2013, August 12)

In photoautotophically air-grown microalgae, CO2 availability is usually limited. The unicellular green algae Chlamydomonas reinhardtii can adapt to low CO2 concentration with the inorganic carbon ... [more ▼]

In photoautotophically air-grown microalgae, CO2 availability is usually limited. The unicellular green algae Chlamydomonas reinhardtii can adapt to low CO2 concentration with the inorganic carbon concentration mechanism (CCM). This has been extensively studied in the past but functional adaptation of the photosynthetic apparatus has been less studied. Photosynthetic organisms can cope with CO2 limitation by dissipating excess absorbed energy with the help of different energy dissipating mechanisms like energy-dependent non-photochemical quenching (NPQ). In Chlamydomonas reinhardtii, this process only seems to develop to high levels in extreme conditions combining high light and strong CO2 limitation. Under moderate CO2 limitation, absence of important energy-dependent NPQ suggests the development of another energy dissipating mechanism. We compared the growth and functional adaptations of the photosynthetic apparatus of the wild-type strain 1690 grown in photobioreactor under low and high CO2 bubbling, 0.039% and 10%, respectively. Under low CO2, where growth was 2 to 4 times slower than under high CO2, the non-linear relationship between electron transport rate (derived from PAM fluorescence measurements) and gross oxygen evolution rate suggested that a significant portion of the electron flux is directed to oxygen at light intensities approaching photosynthetic saturation (either at PSI or at PTOX). The use of the mutant strain PTOX2 indicated that O2 reduction occurs mainly at PSI and not at PTOX. Low temperature fluorescence emission spectra indicated no significant difference in excitation energy distribution between PSI and PSII. Western blot analysis showed no major differences in abundance of Rubisco or of photosystem subunits between the two conditions. In contrast, cytochrome f abundance was lower in high CO2 condition. Although energy-dependent NPQ remained weak, low CO2 cells were characterized by a higher xanthophyll deepoxydation index which usually indicates more dissipation as heat, as also suggested by increased Lhcsr3 expression. Despite a higher ATP requirement of the CCM mechanism in low CO2 condition, only minor difference in cyclic electron transport could be found if compared to high CO2 condition (as determined by P700 spectroscopic measurements). In Chlamydomonas, conflicting views were expressed in earlier studies on the amplitude and role of Mehler-type O2-uptake at steady state. Our analysis of oxygen evolution, electron transport and NPQ after growth under different combinations of light intensities and CO2 supply rates allows us to define Mehler-type alternative electron transport as an important and flexible response to photosynthetic electron transport saturation in Chlamydomonas. [less ▲]

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See detailModelization of photosynthetic and respiratory maximal activities as a function of culture parameters in Chlamydomonas reinhardtii using design of experiment and data analysis by JMP (SAS Institute)
Gerin, Stéphanie ULg; Mathy, Grégory; Franck, Fabrice ULg

Poster (2013, August)

We aimed to modelize the dependence of photosynthetic as well as cytochromial and alternative respiratory maximal activities upon different culture parameters, i.e. light intensity and acetate, carbon ... [more ▼]

We aimed to modelize the dependence of photosynthetic as well as cytochromial and alternative respiratory maximal activities upon different culture parameters, i.e. light intensity and acetate, carbon dioxide, nitrate and ammonium concentrations, in the model unicellular green alga Chlamydomonas reinhardtii. Culture parameters were submitted to a dedicated statistical software (JMP 10.0, SAS Institute) for the generation of a design of experiment. Measurements of oxygen concentrations were carried out to quantify maximal activities. JMP was further used to detect culture parameters exerting a statistically significant effect on maximal activities and to modelize the dependence of maximal activities upon these culture parameters of interest. [less ▲]

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See detailAnalysis of PSII antenna size heterogeneity of Chlamydomonas reinhardtii during state transitions - Colloque annuel de la Société Française de Photosynthèse
de Marchin, Thomas ULg; Ghysels, Bart ULg; Nicolay, Samuel ULg et al

Conference (2013, June 18)

PSII antenna size heterogeneity has been extensively studied in the past. Based on in vivo DCMU fluorescence rise kinetics, at least two types of photosystems were described. They differ by their apparent ... [more ▼]

PSII antenna size heterogeneity has been extensively studied in the past. Based on in vivo DCMU fluorescence rise kinetics, at least two types of photosystems were described. They differ by their apparent antenna size and connectivity (this last term refers to the transfer of absorbed energy from a closed PSII unit to an open neighboring unit). In this study, we analysed PSII heterogeneity in Chlamydomonas reinhardtii using non-linear linear regression fitting on in vivo DCMU fluorescence rise kinetics, with a focus on changes in PSII heterogeneity associated with state transitions. We found that PSIIα possesses a high degree of connectivity and an antenna about 3 times larger than PSIIβ, as described previously. In contrast with most earlier studies, we found some connectivity for PSIIβ (although it was highly variable). This is in agreement with recent models based on biochemical and structural analysis of PSII after gel filtration separation which describe PSII mega-, super- and core- complexes in Chlamydomonas. According to these studies, the smallest unit of PSII in vivo would be a dimer of two core complexes hence still allowing connectivity. We also showed that strain and medium dependent variations in the half-time of the fluorescence rise, generally taken as an indicator of the average cross-section of PSII, can be explained by variations in the proportions of PSIIα and PSIIβ. When analyzing the state transition process, we showed for the first time in vivo that it induces an inter-conversion of PSIIα and PSIIβ. These findings are discussed with respect to the latest insights on the remodeling of the pigment-protein PSII architecture during this process. [less ▲]

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See detailLes microalgues: un défi énergétique
Franck, Fabrice ULg

Conference (2013, June 06)

Les microalgues sont des micro-organismes photosynthétiques dont la culture commerciale en masse est pratiquée depuis les années '60. Les rendements en biomasse de la culture de microalgues sont ... [more ▼]

Les microalgues sont des micro-organismes photosynthétiques dont la culture commerciale en masse est pratiquée depuis les années '60. Les rendements en biomasse de la culture de microalgues sont supérieurs à ceux de cultures traditionnelles, et elle peut s'effectuer sur des terrains impropres à l'agriculture traditionnelle. Les perspectives d'utilisation de la biomasse microalgale pour la production de biocarburants ont suscité d'importants efforts de recherche au cours des années récentes. Les recherches en cours visent à améliorer les souches d'algues utilisables ainsi que différents aspects des procédés de production, de traitement et d'extraction de la biomasse algale afin d'aboutir à moyen terme à un procédé durable et économiquement viable. [less ▲]

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See detailFunction of the Chloroplast Hydrogenase in the Microalga Chlamydomonas: The Role of Hydrogenase and State Transitions during Photosynthetic Activation in Anaerobiosis
Ghysels, Bart ULg; Godaux, Damien ULg; Matagne, René-Fernand ULg et al

in PLoS ONE (2013), 8(5), 64161

Like a majority of photosynthetic microorganisms, the green unicellular alga Chlamydomonas reinhardtii may encounter O2 deprived conditions on a regular basis. In response to anaerobiosis or in a ... [more ▼]

Like a majority of photosynthetic microorganisms, the green unicellular alga Chlamydomonas reinhardtii may encounter O2 deprived conditions on a regular basis. In response to anaerobiosis or in a respiration defective context, the photosynthetic electron transport chain of Chlamydomonas is remodeled by a state transition process to a conformation that favours the photoproduction of ATP at the expense of reductant synthesis. In some unicellular green algae including Chlamydomonas, anoxia also triggers the induction of a chloroplast-located, oxygen sensitive hydrogenase, which accepts electrons from reduced ferredoxin to convert protons into molecular hydrogen. Although microalgal hydrogen evolution has received much interest for its biotechnological potential, its physiological role remains unclear. By using specific Chlamydomonas mutants, we demonstrate that the state transition ability and the hydrogenase function are both critical for induction of photosynthesis in anoxia. These two processes are thus important for survival of the cells when they are transiently placed in an anaerobic environment. [less ▲]

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See detailA dual strategy to cope with high light in Chlamydomonas reinhardtii
Allorent, G; Tokutsu, R; Roach, T et al

in Plant Cell (2013), 25(2), 545-557

Absorption of light in excess of the capacity for photosynthetic electron transport is damaging to photosynthetic organisms. Several mechanisms exist to avoid photodamage, which are collectively referred ... [more ▼]

Absorption of light in excess of the capacity for photosynthetic electron transport is damaging to photosynthetic organisms. Several mechanisms exist to avoid photodamage, which are collectively referred to as nonphotochemical quenching. This term comprises at least two major processes. State transitions (qT) represent changes in the relative antenna sizes of photosystems II and I. High energy quenching (qE) is the increased thermal dissipation of light energy triggered by lumen acidification. To investigate the respective roles of qE and qT in photoprotection, a mutant (npq4 stt7-9) was generated in Chlamydomonas reinhardtii by crossing the state transition–deficient mutant (stt7-9) with a strain having a largely reduced qE capacity (npq4). The comparative phenotypic analysis of the wild type, single mutants, and double mutants reveals that both state transitions and qE are induced by high light. Moreover, the double mutant exhibits an increased photosensitivity with respect to the single mutants and the wild type. Therefore, we suggest that besides qE, state transitions also play a photoprotective role during high light acclimation of the cells, most likely by decreasing hydrogen peroxide production. These results are discussed in terms of the relative photoprotective benefit related to thermal dissipation of excess light and/or to the physical displacement of antennas from photosystem II. [less ▲]

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