References of "Francis, Frédéric"
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See detailEffect of flower traits and hosts on the abundance of parasitoids in perennial multiple species wildflower strips sown within oilseed rape (Brassica napus) crops
Hatt, Séverin ULiege; Uyttenbroeck, Roel ULiege; Chevalier Mendes Lopes, Thomas et al

in Arthropod-Plant Interactions (in press)

Reducing the use of insecticides is an important issue for agriculture today. Sowing wildflower strips along field margins or within crops represents a promising tool to support natural enemy populations ... [more ▼]

Reducing the use of insecticides is an important issue for agriculture today. Sowing wildflower strips along field margins or within crops represents a promising tool to support natural enemy populations in agricultural landscapes and, thus, enhance conservation biological control. However, it is important to sow appropriate flower species that attract natural enemies efficiently. The presence of prey and hosts may also guide natural enemies to wildflower strips, potentially preventing them from migrating into adjacent crops. Here, we assessed how seven flower traits, along with the abundance of pollen beetles (Meligethes spp., Coleoptera: Nitidulidae) and true weevils (Ceutorhynchus spp., Coleoptera: Curculionidae), affect the density of parasitoids of these two coleopterans in wildflower strips sown in an oilseed rape field in Gembloux (Belgium). Only flower traits, not host (i.e. pollen beetles and true weevils) abundance, significantly affected the density of parasitoids. Flower colour, ultraviolet reflectance and nectar availability were the main drivers affecting parasitoids. These results demonstrate how parasitoids of oilseed rape pests react to flower cues under field conditions. Similar analyses on the pests and natural enemies of other crops are expected to help to develop perennial flower mixtures able to enhance biological control throughout a rotation system. [less ▲]

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See detailPerformances of local poultry breed fed black soldier fly larvae reared on horse manure
Moula, Nassim ULiege; Scippo, Marie-Louise ULiege; Douny, Caroline ULiege et al

in Animal Nutrition (in press)

In poultry, feed based on maggots, like larvae of Black Soldier fly (Hermetia illucens) is an attractive option to substitute current ingredients which are expensive and often in direct or indirect ... [more ▼]

In poultry, feed based on maggots, like larvae of Black Soldier fly (Hermetia illucens) is an attractive option to substitute current ingredients which are expensive and often in direct or indirect competition with human food. Little information is currently available on the utility of these larvae in poultry feed, so goals of this study were to determine whether larvae could be reared on horse manure under traditional farming conditions and to evaluate the growth performances of a local poultry fed these larvae and the fatty acids profiles of their meat. After freezing and thawing, larvae were introduced in the feed of Ardennaise chickens between 30 and 80 days of age. Birds in the control group received a commercial standard feed while those in the treatment group received the same commercial feed in which 8% was substituted with whole fresh larvae corresponding to 2% on a dry matter basis. Mean ± standard errors of larval length and weight were 20.67 ± 2.21 mm and 0.14 ± 0.02 g, respectively. Mean larval percentages of dry matter and of substances extractable in diethyl ether were 24.6% and 23.1%, respectively. Larval fatty acids profiles were predominantly composed of lauric (28.1%) and palmitic (22.0%) acids. Least squares means of weekly weights of chicken, adjusted for the effects of sex, replication and initial weights, were significantly higher (P < 0.05) by 77.03 ± 53.37 g in larvae-fed than in control chickens. All the other measurements were not statistically different between larvae-fed and control chicken, including fatty acid profiles, protein content and ω6/ω3 ratio. In conclusion, the use of black soldier fly larvae in the diet of local chicken breed may be an alternative to the use of soy. [less ▲]

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See detailInsects, the next European foodie craze?
Caparros Megido, Rudy ULiege; Haubruge, Eric ULiege; Francis, Frédéric ULiege

in Edible Insects in Sustainable Food Systems (in press)

Edible insects are systematically targeted as a major future food for European consumers but success in introducing entomophagy in Western society depends on factors governing consumers’ attitudes towards ... [more ▼]

Edible insects are systematically targeted as a major future food for European consumers but success in introducing entomophagy in Western society depends on factors governing consumers’ attitudes towards insect-based products. Effectively, as for sushi in the 2000s, edible insects are considered as new food products in Europe and are deeply related to a fear or a reject feeling by consumers called “food neophobia“. Consequently, several studies have been achieved to face the actual aversion of European consumers for insects. These studies principally tried to understand the insect-related neophobia, to highlight positive arguments for entomophagy development and also to test possible ways of integration of insects as food. The purpose of this chapter is to get an overview of the actual literature on edible insect acceptability in Europe to propose conceivable solutions for product development and new approaches for further studies. [less ▲]

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See detailEfficacy of Basil-Cabbage intercropping to control insect pests in Benin, West Africa
Yarou, Boni Barthélémy ULiege; Assogba Komlan, Françoise; Tossou, Eric et al

in Communications in Agricultural and Applied Biological Sciences (in press)

Cabbage (Brassica oleracea L.) is a common vegetable whose production is severely limited by insect pest pressure in Western Africa. This study was conducted during the dry and rainy season in the south ... [more ▼]

Cabbage (Brassica oleracea L.) is a common vegetable whose production is severely limited by insect pest pressure in Western Africa. This study was conducted during the dry and rainy season in the south Benin to evaluate the potential of tropical basil (Ocimum gratissimum L.) for repelling cabbage pests (Hellula undalis Fabricius, Plutella xylostella L. and Spodoptera litto-ralis Boisduval). In a complete randomized block design with four replicates, the insect infestation were compared between three association modalities: (i) cabbage intercropped with tropical basil, (ii) cabbage plots surrounded by tropical basil and (iii) control (no basil in the cabbage area). The presence of tropical basil near cabbage plots significantly reduced insect pest abundance on cabbage. Damages were also less important on cabbage intercropped with tropical basil. Among both intercropping modalities, alternate rows showed the best results in terms of reducing pest populations and damages than compared to cabbage plots surrounded by tropical basil. [less ▲]

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See detailA push–pull strategy to control aphids combines intercropping with semiochemical releases
Xu, Qingxuan ULiege; Hatt, Séverin ULiege; Chevalier Mendes Lopes, Thomas ULiege et al

in Journal of Pest Science (in press)

Even if insect pest populations can be reduced by increasing plant diversity through intercropping, natural enemies are not always favored in such systems. Alternatively, semiochemical substances have ... [more ▼]

Even if insect pest populations can be reduced by increasing plant diversity through intercropping, natural enemies are not always favored in such systems. Alternatively, semiochemical substances have been tested to enhance biological control, with inconsistent results. Combining both strategies can be an interesting way to maximize pest control. In this work, a 2-year setup involving wheat–pea strip intercropping combined with the release of E-b-farnesene (EBF) or methyl salicylate (MeSA) was tested as a push–pull strategy to simultaneously repel aphids and attract beneficials. Two types of slow-release formulation (i.e., oil and alginate beads) containing EBF or MeSA were deployed with the intercropping. The abundance of aphids was significantly decreased, while hoverfly larvae and mummified aphids increased on both pea plants and wheat tillers by the release of oil-formulated EBF and MeSA. The proportion parasitism of the aphid-parasitism rate [mummies/(aphids + mummies)] was also increased by treating both crops in both years. Releasing EBF through oil rather than alginate beads proved significantly better for attracting natural enemies and reducing aphids. Aphids were negatively correlated with the density of hoverflies (both adults and larvae) and numbers of mummies. All these results showed that combining intercropping with the release of EBF or MeSA formulated in oil can significantly reduce aphid density and attract their natural enemies. Therefore, the combination of both strategies could help farmers reduce the use of insecticides. [less ▲]

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See detailDevelopment of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens
Marien, Aline; Debode, Frédéric; Aerts, Céline et al

Poster (2017, October 01)

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See detailLes savoirs paysans pour coordonner la conservation de la nature et l’agriculture - Piste pour une prise en compte renforcée des compétences des agriculteurs dans l’action agro-environnementale.
Brédart, David; Hatt, Séverin ULiege; Méhu, Marie et al

in Mille Lieux (2017), 7

Lors d’un travail de recherche, nous avons rencontré des agriculteurs de la zone limoneuse en Wallonie, un territoire de culture des plus productifs. Ces agriculteurs ont en commun d’avoir installé des ... [more ▼]

Lors d’un travail de recherche, nous avons rencontré des agriculteurs de la zone limoneuse en Wallonie, un territoire de culture des plus productifs. Ces agriculteurs ont en commun d’avoir installé des bandes aménagées en bordure de parcelles dans le cadre des méthodes agro-environnementales (MAEC - dispositifs de la Politique Agricole Commune visant à protéger l’environnement dans les espaces agricoles). Nous avons tenté de comprendre ce que sont et ce que pourraient devenir les bandes aménagées pour ces agriculteurs. En effet, alors que de nombreux travaux se sont intéressés aux aspects techniques, notamment à la composition et à la gestion des mélanges semés, peu d’attention a été portée aux agriculteurs et à la façon dont ils intègrent ces mélanges dans leur pratique. Nous explorons, à travers notre analyse, la possibilité d’une co-construction des MAEC avec les agriculteurs pour une action agro-environnementale pertinente aussi du point de vue de la production agricole. [less ▲]

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See detailPlantes pesticides et protection des cultures maraichères en Afrique de l’Ouest (synthèse bibliographique)
Yarou, Boni Barthélémy ULiege; Silvie, Pierre; Assogba Komlan, Françoise et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 21(4), 288-304

Introduction. Les cultures maraichères occupent une place importante pour l’alimentation humaine et contribuent significativement aux revenus des familles en Afrique de l’Ouest, mais leur production est ... [more ▼]

Introduction. Les cultures maraichères occupent une place importante pour l’alimentation humaine et contribuent significativement aux revenus des familles en Afrique de l’Ouest, mais leur production est confrontée à une pression des bioagresseurs qui limite leur productivité. Littérature. Cette revue, basée sur des publications scientifiques, a pour objectif d’évaluer le potentiel des plantes pesticides comme alternative à l’usage des pesticides de synthèse pour lutter contre les bioagresseurs des cultures maraichères en Afrique de l’Ouest. Elle montre que le principal moyen actuel pour combattre ces bioagresseurs repose essentiellement sur les pesticides de synthèse. Cependant, compte tenu de leur nocivité sur l’homme et l’environnement, en plus de la sélection de populations résistantes chez les bioagresseurs, la recherche de solutions alternatives s’impose. Les plantes pesticides se présentent comme une alternative prometteuse dans le contexte ouest-africain. En effet, diverses espèces de plantes pesticides peuvent être utilisées comme extraits de plantes ou en association avec d’autres cultures pour le contrôle des bioagresseurs. Celles-ci sont présentées dans le présent article de synthèse. Conclusions. Les plantes pesticides peuvent être une alternative prometteuse pour la gestion des bioagresseurs des cultures maraichères. Cependant, la plupart d’entre elles ne sont pas cultivées. Explorer les capacités biocides de plantes d’intérêt comme le genre Ocimum, connu pour ses usages thérapeutique, médicinal et alimentaire pourrait être intéressant pour le producteur. Toutefois, les populations doivent être sensibilisées sur les avantages à long terme des produits traités avec les pesticides à base de plantes pour faciliter leur utilisation. [less ▲]

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See detailEffects of wildflower strips and an adjacent forest on aphids and their natural enemies in a pea field
Hatt, Séverin ULiege; Mouchon, Pierre; Chevalier Mendes Lopes, Thomas ULiege et al

in Insects (2017), 8(3), 99

Landscape diversification is a key element for the development of sustainable agriculture. This study explores whether the implementation of habitats for pest natural enemies enhances conservation ... [more ▼]

Landscape diversification is a key element for the development of sustainable agriculture. This study explores whether the implementation of habitats for pest natural enemies enhances conservation biological control in an adjacent field. In the present study conducted in Gembloux (Belgium) in 2016, the effect of two different habitats (wildflower strips and a forest) and aphid abundance on the density of aphid natural enemies, mummified aphids and parasitism on pea plants was assessed through visual observations. The effect of the habitats on aphids was also evaluated. The habitats but not aphid density significantly affected hoverfly larvae, which were more abundant adjacent to wildflower strips than to the forest. The contrary was observed for ladybeetle adults, which were positively related with aphids but not affected by the adjacent habitats. The abundance of mummies and the parasitism rate were significantly affected by both the habitats and aphid density. They were both significantly enhanced adjacent to wildflower strips compared to the forest, but the total parasitism rate was low (<1%), questioning whether parasitoids could significantly control aphids on the pea crop. As for the aphids, their abundance was not significantly affected by the adjacent habitats. These results are discussed with respect to the potential of these habitats to provide overwintering sites and food resources for natural enemies, and thereby enhance conservation biological control. [less ▲]

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See detailOviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae)
Yarou, Boni Barthélémy ULiege; Bawin, Thomas; Boullis, Antoine ULiege et al

in Environmental Science and Pollution Research (2017)

The leafminer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yields by up to 100% in greenhouses and fields, in several countries globally ... [more ▼]

The leafminer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yields by up to 100% in greenhouses and fields, in several countries globally. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and the health of producers, alternative control methods are needed. In this study, we assessed the oviposition-deterring effect of basil plants, Ocimum gratissimum L. and O. basilicum L. (Lamiaceae), using dual-choice behavioural assays performed in flight tunnels. We found that both plants significantly reduced T. absoluta oviposition behaviour on a tomato plant located nearby. To evaluate the potential effect of basil volatile organic compounds, we formulated essential oils of both plant species in paraffin oil, and observed a similar oviposition-deterring effect. Gas chromatography analyses detected 18 constituents in these essential oils which the major constituents included thymol (33.3%), p-cymene (20.4%), γ-terpinene (16.9%), myrcene (3.9%) in O. gratissimum and estragol (73.8%), linalool (8.6%), β-elemene (2.9%) and E-β-ocimene (2.6%) in O. basilicum. Twenty and 33 compounds were identified of the volatiles collected on O. gratissimum and O. basilicum plants, respectively. The main components include the following: p-cymene (33.5%), γ-terpinene (23.6%), α-terpinene (7.2%), α-thujene (6.7%) and E-α-bergamotene (38.9%) in O. gratissimum, and methyl eugenol (26.1%), E-β-ocimene (17.7%), and linalool (9.4%) in O. basilicum. Four compounds (α-pinene, β-pinene, Myrcene, Limonene) were common in essential oils and plants. Our results suggest the valuable potential of basil and associated essential oils as a component of integrated management strategies against the tomato leafminer. [less ▲]

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See detailLe maraîchage périurbain à Libreville et Owendo (Gabon) : pratiques culturales et durabilité
Bayendi-Loudit, Sandrine ULiege; Ndoutoume Ndong, Auguste; Francis, Frédéric ULiege

in Cahiers Agricultures (2017)

In Gabon, peri-urban gardening is an opportunity to provide vegetables to the main cities, such as Libreville and Owendo. Following a survey conducted in three market gardening areas, an inventory was ... [more ▼]

In Gabon, peri-urban gardening is an opportunity to provide vegetables to the main cities, such as Libreville and Owendo. Following a survey conducted in three market gardening areas, an inventory was conducted on the socio-economic characteristics, the diversity of crops, and pesticide uses. The cropped areas range from 0.08 ha to 0.4 ha per farmer, according to the site. National operators represent 51%, while people from Burkina Faso manage 40% of vegetable production. The most cultivated species throughout the year are amaranth (Amaranthus hybridus L.), lettuce (Lactuca sativa L.), Guinea sorrel (Hibiscus sabdariffa L.) and black nightshade (Solanum nigrum L.). The most important pests are Aphididae and some beetles. The most commonly used plant protection products are insecticides, mainly conventional neurotoxic. Best crop monitoring, pest control including pesticide application reduction, and the possibility to offer microcredit systems to small producers would help increasing peri-urban healthy vegetable production and increase local food autonomy [less ▲]

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See detailCan we use semiochemicals to control Aphis craccivoraKoch?
Bayendi-Loudit, Sandrine ULiege; Yarou, Boni Barthélémy ULiege; Boullis, Antoine ULiege et al

Poster (2017, July)

Amaranthus hybridus L. (Amaranthaceae) is among the most important vegetable crop in several sub-Saharan countries. Aphis craccivora Koch (Homoptera: Aphididae) is an aphid species that cause significant ... [more ▼]

Amaranthus hybridus L. (Amaranthaceae) is among the most important vegetable crop in several sub-Saharan countries. Aphis craccivora Koch (Homoptera: Aphididae) is an aphid species that cause significant losses in absence of chemical control. In this laboratory research, we evaluate the efficiency of informative substances (E-b-farnesene, methyl salicylate, essential oil of Ocimum gratissimum L. and Ocimum basilicum L.) to repel aphids from their host plant. Our assays were carried out in plastic trays containing twenty-five amaranth plants. In each tray, twenty apterous A. craccivora adults were placed on the central plant (release plant) associated with a semiochemical releaser containing 100 µl of the substance to be tested diluted in paraffin oil. The number of aphids was then counted on each plant surrounding plants every three-days for twelve days. We observed a significant repulsive effect of O. gratissimum and O. basilicum essential oils, that considerably reduced the aphid population on the treated plant, compared to control. However, no repulsive effect of E-b-farnesene and methyl salicylate on aphids was observed. Bioactivities of these substances and their using as alternative aphicidal in integrated pest management are discussed [less ▲]

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See detailDispersion of Myzus persicae and transmission of Potato Virus Y under elevated CO2 atmosphere
Bosquée, Emilie ULiege; Boullis, Antoine ULiege; Bertaux, Morgane et al

Poster (2017, July)

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See detailImpact of endophytic entomopathogenic fungus on plant attractivity in aphid-virus-plant interactions
Fingu Mabola, Junior Corneille ULiege; Bosquée, Emilie ULiege; Boullis, Antoine ULiege et al

Poster (2017, July)

Endophytic entomopathogenic fungi (EEPF) are biological control agents able to live in their host plants in a symbiotic interaction and secrete secondary metabolites in the host plant tissues interacting ... [more ▼]

Endophytic entomopathogenic fungi (EEPF) are biological control agents able to live in their host plants in a symbiotic interaction and secrete secondary metabolites in the host plant tissues interacting with pests and phytopathogenic agents like plants virus. This is the case of Barley yellow dwarf virus (BYDV) transmitted by the Bird cherry oat aphid (Rhopalosiphum padi Linnaeus) and the Grain aphid (Sitobion avenae Fabricius). This virus is one of the most economically important phytoviruses affecting major world cereal crops such as wheat, rice and corn, causing significant yield losses. In this study, we investigated the influence of EEPF presence in the plant tissues on the vector’s orientation preference by considering the insect virus-infection status. We performed a choice test in Petri dishes and collection of volatile compounds emitted by plants. The impact of these interactions between plant-fungi-virus is finally discussed. [less ▲]

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See detailInsect fatty acids: A comparison of lipids from three Orthopterans and Tenebrio molitor L. larvae
Paul, Aman ULiege; Frederich, Michel ULiege; Caparros Megido, Rudy ULiege et al

in Journal of Asia-Pacific Entomology (2017), 20(2), 337-340

In order to explore some potential insect sources of food lipids, the lipid compositions of three Orthopterans (Acheta domesticus, Conocephalus discolor and Chorthippus parallelus) were analyzed and ... [more ▼]

In order to explore some potential insect sources of food lipids, the lipid compositions of three Orthopterans (Acheta domesticus, Conocephalus discolor and Chorthippus parallelus) were analyzed and compared with those of Tenebrio molitor larvae. A. domesticus, Co. discolor, Ch. parallelus and T. molitor larvae were found to contain approximately 15%, 13%, 10% and 32% lipids on dry weight, respectively. The lipids from three Orthopterans contain much higher amounts of essential fatty acids than those of T. molitor larvae. The two Orthopterans of the suborder Ensifera i.e., A. domesticus and Co. discolor contain linoleic acid in major quantities, while Ch. parallelus of the suborder Caelifera, contain α-linolenic acid in major quantities. The consumption of linoleic and α-linolenic fatty acid is linked with numerous health promoting effects. The factors that contribute to differences in fatty acid profiles of these insects are being discussed. At last the nutritional parameters including polyunsaturated to saturated and omega 6 to omega 3 fatty acid ratios of these insect lipids are also being discussed to understand the potential role of these lipids in human nutrition. [less ▲]

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See detailMilk-clotting enzymes from wild cardoon flowers: identification and technological characterization
Ben Amira, Amal; Bauwens, Julien; De Pauw, Edwin ULiege et al

Conference (2017, April 12)

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See detailGeneration of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes
Sun, Yongwei ULiege; Xia, Lanqin; Francis, Frédéric ULiege et al

in Frontiers in Plant Science (2017), 8

Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching ... [more ▼]

Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0% and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. [less ▲]

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See detailStress indicator gene expression profiles, colony dynamics and tissue development of honey bees exposed to sub-lethal doses of imidacloprid in laboratory and field experiments
De Smet, Lina; Hatjina; Ioannidis, Pavlos et al

in PLOS One (2017), 10(137),

In this study, different context-dependent effects of imidacloprid exposure on the honey bee response were studied. Honey bees were exposed to different concentrations of imidacloprid during a time period ... [more ▼]

In this study, different context-dependent effects of imidacloprid exposure on the honey bee response were studied. Honey bees were exposed to different concentrations of imidacloprid during a time period of 40 days. Next to these variables, a laboratory-field comparison was conducted. The influence of the chronic exposure on gene expression levels was determined using an in-house developed microarray targeting different immunity-related and detoxification genes to determine stress-related gene expression changes. Increased levels of the detoxification genes encoding, CYP9Q3 and CYT P450, were detected in imidacloprid- exposed honey bees. The different context-dependent effects of imidacloprid exposure on honey bees were confirmed physiologically by decreased hypopharyngeal gland sizes. Honey bees exposed to imidacloprid in laboratory cages showed a general immunosuppression and no detoxification mechanisms were triggered significantly, while honey bees infield showed a resilient response with an immune stimulation at later time points. However, the treated colonies had a brood and population decline tendency after the first brood cycle in the field. In conclusion, this study highlighted the different context-dependent effects of imidacloprid exposure on the honey bee response. These findings warn for possible pitfalls concerning the generalization of results based on specific experiments with short exposure times. The increased levels of CYT P450 and CYP9Q3 combined with an immune response reaction can be used as markers for bees which are exposed to pesticides in the field. [less ▲]

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See detailStructure and distribution of the sensilla on the antennae of Tuta absoluta (Lepidoptera: Gelechiidae)
Bawin, Thomas ULiege; Collard, France ULiege; De Backer, Lara ULiege et al

in Micron (2017), 96

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a widespread devastating pest that develops on tomato and other economically important solanaceous crops. Current semiochemically-based ... [more ▼]

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a widespread devastating pest that develops on tomato and other economically important solanaceous crops. Current semiochemically-based management strategies still fail to significantly reduce damages and need to be improved. Here we describe under scanning and transmission electron microscopy the structure and distribution of the sensilla that are displayed on adult antennae. These were similar in size between males (3424.4 ± 135.3 μm) and females (3292.1 ± 111.5 μm), being segmented into a scape, a pedicel, and a distal filiform flagellum. Eight morphological sensilla types were observed on both sexes: Böhm’s bristles, sensilla squamiformia, sensilla trichodea, sensilla basiconica (two subtypes), sensilla chaetica, sensilla coeloconica, sensilla auricillica, and sensilla styloconica. The main sexual dimorphism was related to the higher abundance of sensilla trichodea in males, twice as abundant as in females. The putative functional significance of the different sensilla types regarding the insect ecology is discussed based on the available literature. This work provides descriptions of the antennae and related sensory structures. We expect these results to help develop further electrophysiological investigations aiming to a better understanding of T. absoluta olfaction. [less ▲]

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See detailMicrobiological load of edible insects found in Belgium
Caparros Megido, Rudy ULiege; Desmedt, Sandrine; Blecker, Christophe ULiege et al

in Insects (2017)

Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are ... [more ▼]

Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are sourcing fresh insects from fishing stores or towards traditional markets to find exotic insects that are illegal and not sanitarily controlled. From this perspective, this study aims to characterize the microbial load of edible insects found in Belgium (i.e., fresh mealworms and house crickets from European farms and smoked termites and caterpillars from a traditional Congolese market) and to evaluate the efficiency of different processing methods (blanching for all species and freeze-drying and sterilization for European species) in reducing microorganism counts. All untreated insect samples had a total aerobic count higher than the limit for fresh minced meat (6.7 log cfu/g). Nevertheless, a species-dependent blanching step has led to a reduction of the total aerobic count under this limit, except for one caterpillar species. Freeze-drying and sterilization treatments on European species were also effective in reducing the total aerobic count. Yeast and mold counts for untreated insects were above the Good Manufacturing Practice limits for raw meat, but all treatments attained a reduction of these microorganisms under this limit. These results confirmed that fresh insects, but also smoked insects from non-European trades, need a cooking step (at least composed of a first blanching step) before consumption. Therefore, blanching timing for each studied insect species is proposed and discussed. [less ▲]

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