References of "Fievez, Virginie"
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See detailNeutralizing properties of peptides derived from CXCR4 extracellular loops towards CXCL12 bidnig and HIV-1 infection
Szpakowska, Martyna ULg; Fievez, Virginie; Counson, Manuel et al

Poster (2014, April)

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See detailNeutralising properties of peptides derived from CXCR4 extracellular loops towards CXCL12 binding and HIV-1 infection
Chevigné, Andy; Fievez, Virginie; Szpakowska, Martyna ULg et al

in Biochimica et Biophysica Acta-Molecular Cell Research (2014)

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See detailFunction, diversity and therapeutic potential of the N-terminal domain of human chemokine receptors
Szpakowska, Martyna ULg; Fievez, Virginie; Arumugan, Karthik et al

in Biochemical Pharmacology (2012)

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See detailEngineering and characterisation of chimeric CXCR4 and CXCR7 chemokine receptors
Szpakowska, Martyna ULg; Fievez, Virginie; Counson, Manuel et al

Poster (2012, January)

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See detailTargeting nanoparticles to M cells with non-peptidic ligands for oral vaccination
Freichels, Hélène ULg; Fievez, Virginie; Plapied, Laurence et al

Poster (2010, March 18)

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See detailAcylated and unacylatedghrelin binding to membranes and to ghrelin receptor: Towards a better understanding of the underlying mechanisms
Staes, Edith; Absil, Pierre-Antoine; Lins, Laurence ULg et al

in Biochimica et Biophysica Acta - Biomembranes (2010), 1798

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See detailTargeting nanoparticles to M cells with non-peptidic ligands for oral vaccination
Fievez, Virginie; Plapied, Laurence; des Rieux, Anne et al

in European Journal of Pharmaceutics & Biopharmaceutics (2009)

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis ... [more ▼]

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis that non-peptidic ligands targeting intestinal M cells or APCs would be more efficient for oral immunization than RGD, novel non-peptidic and peptidic analogs (RGD peptidomimitic (RGDp), LDV derivative (LDVd) and LDV peptidomimetic (LDVp)) as well as mannose were grafted on the PEG chain of PCL–PEG and incorporated in PLGA-based nanoparticles. RGD and RGDp significantly increased the transport of nanoparticles across an in vitro model of human M cells as compared to enterocytes. RGD, LDVp, LDVd and mannose enhanced nanoparticle uptake by macrophages in vitro. The intraduodenal immunization with RGDp-, LDVd- or mannose-labeled nanoparticles elicited a higher production of IgG antibodies than the intramuscular injection of free ovalbumin or intraduodenal administration of either non-targeted or RGD-nanoparticles. Targeted formulations were also able to induce a cellular immune response. In conclusion, the in vitro transport of nanoparticles, uptake by macrophages and the immune response were positively influenced by the presence of ligands at the surface of nanoparticles. These targeted-nanoparticles could thus represent a promising delivery system for oral immunization. [less ▲]

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See detailFunctional amphiphilic and degradable copolymers for drug delivery systems
Freichels, Hélène ULg; Pourcelle, Vincent; Plapied, Laurence et al

Poster (2008, December 18)

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See detailPEGylated PLGA-based nanoparticles targeting M cells for oral vaccination
Garinot, Marie; Fievez, Virginie; Pourcelle, Vincent et al

in Journal of Controlled Release (2007), 120(3), 195-204

To improve the efficiency of orally delivered vaccines, PEGylated PLGA-based nanoparticles displaying RGD molecules at their surface were designed to target human M cells. RGD grafting was performed by an ... [more ▼]

To improve the efficiency of orally delivered vaccines, PEGylated PLGA-based nanoparticles displaying RGD molecules at their surface were designed to target human M cells. RGD grafting was performed by an original method called "photografting" which covalently linked RGD peptides mainly on the PEG moiety of the PCL-PEG, included in the formulation. First, three non-targeted formulations with size and zeta potential adapted to M cell uptake and stable in gastro-intestinal fluids, were developed. Their transport by an in vitro model of the human Follicle associated epithelium (co-cultures) was largely increased as compared to mono-cultures (Caco-2 cells). RGD-labelling of nanoparticles significantly increased their transport by co-cultures. due to interactions between the RGD ligand and the I intregrins detected at the apical surface of co-cultures. In vivo studies demonstrated that RGD-labelled nanoparticles particularly concentrated in M cells. Finally, ovalbumin-loaded nanoparticles were orally administrated to mice and induced an IgG response, attesting antigen ability to elicit an immune response after oral delivery. [less ▲]

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See detailHelodermin-loaded nanoparticles: Characterization and transport across an in vitro model of the follicle-associated epithelium
des Rieux, Anne; Fievez, Virginie; Momtaz, Maryam et al

in Journal of Controlled Release (2007), 118(3), 294-302

M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of ... [more ▼]

M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of M cells on the transport of free and encapsulated helodermin - a model peptide - across the intestinal epithelium. M cells enhanced transport of intact helodermin (18-fold, Papp 3 X 10(-6) cm s(-1)). As pegylation increased nanoparticle transport by M cells, helodermin was encapsulated in 200 mu nanoparticles containing PEG-b-PLA:PLGA 1:1. Stability of the selected formulation was demonstrated in simulated gastric and intestinal fluids. M cells increased the transport of helodermin encapsulated in these nanoparticles by a factor of 415, as compared to Caco-2 cells. Transport of free and encapsulated helodermin occurred most probably by endocytosis. In conclusion, M cells improved helodermin transport across the intestinal epithelium, confirming their high potential for oral delivery of peptides. [less ▲]

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