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See detailPhenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law
Baert, Jonathan ULg; Kinet, Romain ULg; Brognaux, Alison ULg et al

in Biotechnology Journal (in press)

Noise in gene and protein expression is a major cause for bioprocess deviation. However, this phenomenon has been only scarcely considered in real bioprocessing conditions. In this work, a scaling-law ... [more ▼]

Noise in gene and protein expression is a major cause for bioprocess deviation. However, this phenomenon has been only scarcely considered in real bioprocessing conditions. In this work, a scaling-law derived from a genome-scale studies based on GFP reporter systems has been calibrated to an on-line flow cytometry device, allowing thus to get an insight at the level of promoter activity and associated noise during a whole microbial culture carried out in bioreactor. We show that most of the GFP reporter systems investigated and thus corresponding genes could be included inside the area covered by the scaling-law. The experimental results suggest that this scaling-law could be used to predict the dynamics of promoter activity, as well as the associated noise, in bioprocessing conditions. The knowledge acquired throughout this work could be used for the design of more robust expression systems. [less ▲]

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See detailFungal lipase production by solid-state fermentation
Ramos-Sanchez, L.B.; Julian-Ricardo, M.C.; Cordova, J. et al

Conference (2014, December)

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See detailBacillus as a source of novel antibiotics
Fickers, Patrick ULg

Conference (2014, November)

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See detailPropuesta de estrategia para la producción de biocombustibles de biomasa lignocelulósica
Ramos-Sanchez, L.B.; Cujilema-Quitio, M.C.; Leon-Revelo, G. et al

Conference (2014, November)

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See detailOptimization of pAOX1 induction in a methanol/sorbitol co-feeding process in Pichia pastoris
Carly, F.; Niu, H.; Fickers, Patrick ULg

Conference (2014, October)

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See detailAccumulation lipidique par Yarrowia lipolytica: un nouvel outil d'observation
Bouchedja, Doria Naïla; Delvigne, Frank ULg; Boudjellal, Abdelghani et al

Conference (2014, April 06)

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See detailAntibiotic compounds from Bacillus: why are they so amazing?
Fickers, Patrick ULg

Conference (2014, April)

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See detailPichia pastoris: a workhorse for recombinant protein production
Fickers, Patrick ULg

in Current Research in Microbiology and Biotechnology (2014), (2), 354-363

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See detailMacroscopic modelling of baker’s yeast production in fed-batch cultures and its link with trehalose production
Richelle, A.; Fickers, Patrick ULg; Bogaerts, Ph

in Computers & Chemical Engineering (2014), (61), 220-233

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See detailSynthesis and biological evaluation of potential threonine synthase inhibitors: Rhizocticin A and Plumbemycin A
Gahungu; Arguelles Arias, Anthony ULg; Fickers, Patrick ULg et al

in Bioorganic & Medicinal Chemistry (2013), 21

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See detailISOLATION OF THE ANTIMICROBIAL CYCLIC PEPTIDE SUBTILOSIN A FROM A GUT-ASSOCIATED BACILLUS SUBTILIS STRAIN
Schyns, Ghislain; Serra, Claudia; Henriques, Adriano et al

in American Journal of Biochemistry & Biotechnology (2013), 9(3),

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See detailAntimicrobial Activity of Bacillus amyloliquefaciens ANT1 Toward Pathogenic Bacteria and Mold: Effects on Biofilm Formation
Arguelles Arias, Anthony ULg; Nastro, Rosa Anna; Ongena, Marc ULg et al

in Probiotics and Antimicrobial Proteins (2013)

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See detailThe amylolysin from Bacillus amyloliquefaciens GA1
Fickers, Patrick ULg

Conference (2013, June)

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See detailCharacterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Arguelles Arias, Anthony ULg; Ongena, Marc ULg; Devreese, Bart et al

in PLoS ONE (2013), 8(12),

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria ... [more ▼]

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria, including Listeria monocytogenes, Staphylococcus aureus or Bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. Lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in pre-lantibiotic modifications, regulation, export and immunity. Methodology/Findings: Bacillus amyloliquefaciens GA1 was found to produce an antimicrobial peptide, named amylolysin, active on an array of Gram-positive bacteria, including methicillin resistant S. aureus. Genome characterization led to the identification of a putative lantibiotic gene cluster that comprises a structural gene (amlA) and genes involved in modification (amlM), transport (amlT), regulation (amlKR) and immunity (amlFE). Disruption of amlA led to loss of biological activity, confirming thus that the identified gene cluster is related to amylolysin synthesis. MALDI-TOF and LC-MS analysis on purified amylolysin demonstrated that this latter corresponds to a novel lantibiotic not described to date. The ability of amylolysin to interact in vitro with the lipid II, the carrier of peptidoglycan monomers across the cytoplasmic membrane and the presence of a unique modification gene suggest that the identified peptide belongs to the group B lantibiotic. Amylolysin immunity seems to be driven by only two AmlF and AmlE proteins, which is uncommon within the Bacillus genus. Conclusion/Significance: Apart from mersacidin produced by Bacillus amyloliquefaciens strains Y2 and HIL Y-85,544728, reports on the synthesis of type B-lantibiotic in this species are scarce. This study reports on a genetic and structural characterization of another representative of the type B lantibiotic in B. amyloliquefaciens. Copyright: © 2013 Arguelles Arias et al. [less ▲]

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See detailDynamic modeling of methylotrophic Pichia pastoris culture with exhaust gas analysis: from cellular metabolism to process simulation
Niu, H; Daukandt, M; Rodriguez, Ch et al

in Chemical Engineering Science (2013), (87), 381-392

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See detailMetabolic Pathway Analysis and Reduction for Mammalian Cell Cultures-Towards Macroscopic Modeling
Niu, H; Amribt, Z; Fickers, Patrick ULg et al

in Chemical Engineering Science (2013), (102), 461-473

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