References of "Fickers, Patrick"
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See detailAccumulation lipidique par Yarrowia lipolytica: un nouvel outil d'observation
Bouchedja, Doria Naïla; Delvigne, Frank ULg; Boudjellal, Abdelghani et al

Conference (2014, April 06)

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See detailSynthesis and biological evaluation of potential threonine synthase inhibitors: Rhizocticin A and Plumbemycin A
Gahungu; Arguelles Arias, Anthony ULg; Fickers, Patrick ULg et al

in Bioorganic & Medicinal Chemistry (2013), 21

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See detailISOLATION OF THE ANTIMICROBIAL CYCLIC PEPTIDE SUBTILOSIN A FROM A GUT-ASSOCIATED BACILLUS SUBTILIS STRAIN
Schyns, Ghislain; Serra, Claudia; Henriques, Adriano et al

in American Journal of Biochemistry & Biotechnology (2013), 9(3),

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See detailAntimicrobial Activity of Bacillus amyloliquefaciens ANT1 Toward Pathogenic Bacteria and Mold: Effects on Biofilm Formation
Arguelles Arias, Anthony ULg; Nastro, Rosa Anna; Ongena, Marc ULg et al

in Probiotics and Antimicrobial Proteins (2013)

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See detailBiotechnological application of Yarrowia lipolytica lipase : an overview
Fickers, Patrick ULg; Nicaud, J.M.

in Yarrowia lipolytica: Biotechnological Applications (2013)

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See detailThe lipase from Y. lipolytica : genetics, production, regulation and biochemical characterisation
Fickers, Patrick ULg; Sauveplane, V.; Nicaud, J.M.

in Yarrowia lipolytica: Biotechnological Applications (2013)

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See detailGenome of a Gut Strain of Bacillus subtilis.
Schyns, Ghislain; Serra, Claudia R.; Lapointe, Thomas et al

in Genome announcements (2013), 1(1),

Bacillus subtilis is a Gram-positive, rod-shaped, spore-forming bacterium. We present the genome sequence of an undomesticated strain, BSP1, isolated from poultry. The sequence of the BSP1 genome supports ... [more ▼]

Bacillus subtilis is a Gram-positive, rod-shaped, spore-forming bacterium. We present the genome sequence of an undomesticated strain, BSP1, isolated from poultry. The sequence of the BSP1 genome supports the view that B. subtilis has a biphasic lifestyle, cycling between the soil and the animal gastrointestinal tract, and it provides molecular-level insight into the adaptation of B. subtilis to life under laboratory conditions. [less ▲]

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See detailA quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut(+)/pAOX1-lacZ strain.
Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie et al

in Microbial cell factories (2013), 12

BACKGROUND: One of the main challenges for heterologous protein production by the methylotrophic yeast Pichia pastoris at large-scale is related to its high oxygen demand. A promising solution is a co ... [more ▼]

BACKGROUND: One of the main challenges for heterologous protein production by the methylotrophic yeast Pichia pastoris at large-scale is related to its high oxygen demand. A promising solution is a co-feeding strategy based on a methanol/sorbitol mixture during the induction phase. Nonetheless, a deep understanding of the cellular physiology and the regulation of the AOX1 promoter, used to govern heterologous protein production, during this co-feeding strategy is still scarce. RESULTS: Transient continuous cultures with a dilution rate of 0.023 h(-1) at 25 degrees C were performed to quantitatively assess the benefits of a methanol/sorbitol co-feeding process with a Mut+ strain in which the pAOX1-lacZ construct served as a reporter gene. Cell growth and metabolism, including O2 consumption together with CO2 and heat production were analyzed with regard to a linear change of methanol fraction in the mixed feeding media. In addition, the regulation of the promoter AOX1 was investigated by means of beta-galactosidase measurements. Our results demonstrated that the cell-specific oxygen consumption (qO2) could be reduced by decreasing the methanol fraction in the feeding media. More interestingly, maximal beta-galactosidase cell-specific activity (>7500 Miller unit) and thus, optimal pAOX1 induction, was achieved and maintained in the range of 0.45 ~ 0.75 C-mol/C-mol of methanol fraction. In addition, the qO2 was reduced by 30% at most in those conditions. Based on a simplified metabolic network, metabolic flux analysis (MFA) was performed to quantify intracellular metabolic flux distributions during the transient continuous cultures, which further shed light on the advantages of methanol/sorbitol co-feeding process. Finally, our observations were further validated in fed-batch cultures. CONCLUSION: This study brings quantitative insight into the co-feeding process, which provides valuable data for the control of methanol/sorbitol co-feeding, aiming at enhancing biomass and heterologous protein productivities under given oxygen supply. According to our results, beta-galactosidase productivity could be improved about 40% using the optimally mixed feed. [less ▲]

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See detailCharacterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Arias, A. A.; Ongena, Marc ULg; Devreese, B. et al

in PLoS ONE (2013), 8(12),

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria ... [more ▼]

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria, including Listeria monocytogenes, Staphylococcus aureus or Bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. Lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in pre-lantibiotic modifications, regulation, export and immunity. Methodology/Findings: Bacillus amyloliquefaciens GA1 was found to produce an antimicrobial peptide, named amylolysin, active on an array of Gram-positive bacteria, including methicillin resistant S. aureus. Genome characterization led to the identification of a putative lantibiotic gene cluster that comprises a structural gene (amlA) and genes involved in modification (amlM), transport (amlT), regulation (amlKR) and immunity (amlFE). Disruption of amlA led to loss of biological activity, confirming thus that the identified gene cluster is related to amylolysin synthesis. MALDI-TOF and LC-MS analysis on purified amylolysin demonstrated that this latter corresponds to a novel lantibiotic not described to date. The ability of amylolysin to interact in vitro with the lipid II, the carrier of peptidoglycan monomers across the cytoplasmic membrane and the presence of a unique modification gene suggest that the identified peptide belongs to the group B lantibiotic. Amylolysin immunity seems to be driven by only two AmlF and AmlE proteins, which is uncommon within the Bacillus genus. Conclusion/Significance: Apart from mersacidin produced by Bacillus amyloliquefaciens strains Y2 and HIL Y-85,544728, reports on the synthesis of type B-lantibiotic in this species are scarce. This study reports on a genetic and structural characterization of another representative of the type B lantibiotic in B. amyloliquefaciens. Copyright: © 2013 Arguelles Arias et al. [less ▲]

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See detailProduction of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.
Bechet, Max; Castera-Guy, Joany; Guez, Jean-Sebastien et al

in Bioresource technology (2013), 145

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin ... [more ▼]

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin producer. Compared to the parental strain, both mutants overproduced constitutively mycosubtilin, while BBG125 had lost the ability to synthesize surfactin. Surprisingly, BBG125 was found to produce about 2-fold less mycosubtilin than BBG116 despite an expected higher availability of the cytoplasmic precursors and cofactors pool for biosynthesis. Further physiological characterization of BBG125 also highlighted: (i) a strong influence of temperature on mycosubtilin biosynthesis in BBG125 with a maximal productivity observed at 22 degrees C, compared to 15 and 30 degrees C; (ii) substantial changes in fatty acid profiles and thereby in mycosubtilin isoforms, compared to the wild-type strain; and (iii) the presence of five novel mycosubtilin isoforms. The antifungal activities of the new mix were higher than or equal to those of purified isoforms. [less ▲]

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See detailAntimicrobial Activity of Bacillus amyloliquefaciens ANT1 Toward Pathogenic Bacteria and Mold: Effects on Biofilm Formation
Nastro, R. A.; Arguelles-Arias, A.; Ongena, Marc ULg et al

in Probiotics and Antimicrobial Proteins (2013), 5

The intensive use and misuse of antibiotics over the last decades have generated a strong selective pressure for the emergence of multi-resistant strains and nosocomial infections. Biofilm has been ... [more ▼]

The intensive use and misuse of antibiotics over the last decades have generated a strong selective pressure for the emergence of multi-resistant strains and nosocomial infections. Biofilm has been demonstrated as a key parameter in spreading infections, especially in hospitals and healthcare units. Therefore, the development of novel anti-biofilm drugs is actually of the upmost importance. Here, the antimicrobial and antibiofilm activities toward pathogenic microorganisms of a set of non-ribosomal synthesized peptides and polyketides isolated from Bacillus amyloliquefaciens ANT1 culture supernatant are presented. © 2013 Springer Science+Business Media New York. [less ▲]

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See detailGram-positive antibiotic biosynthesis cluster: a review.
Arguelles Arias, Anthony ULg; Craig, M.; Fickers, Patrick ULg

in Science against microbial pathogens: communicating current research and technological advances (2011)

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See detailThe lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications.
Fickers, Patrick ULg; Marty, Alain; Nicaud, Jean Marc

in Biotechnology advances (2011), 29(6), 632-44

Lipases are serine hydrolases that catalyze in nature the hydrolysis of ester bonds of long chain triacylglycerol into fatty acid and glycerol. However, in favorable thermodynamic conditions, they are ... [more ▼]

Lipases are serine hydrolases that catalyze in nature the hydrolysis of ester bonds of long chain triacylglycerol into fatty acid and glycerol. However, in favorable thermodynamic conditions, they are also able to catalyze reactions of synthesis such as esterification or amidation. The non-conventional yeast Yarrowia lipolytica possesses 16 paralogs of genes coding for lipase. However, little information on all those paralogs has been yet obtained and only three isoenzymes, namely Lip2p, Lip7p and Lip8p have been partly characterized so far. Microarray data suggest that only a few of them could be expressed and that lipase synthesis seems to be dependent on the fatty acid or oil used as carbon source confirming the high adaptation of Y. lipolytica to hydrophobic substrate utilization. This review focuses on the biochemical characterization of those enzymes with special emphasis on the Lip2p lipase which is the isoenzyme mainly synthesized by Y. lipolytica. Crystallographic data highlight that this latter is a lipase sensu stricto with a lid covering the active site of the enzyme in its closed conformation. Recent findings on enzyme conditioning in dehydrated or liquid formulation, in enzyme immobilization by entrapment in natural polymers from either organic or mineral origins are also discussed together with long-term storage strategies. The development of various biotechnological applications in different fields such as cheese ripening, waste treatment, drug synthesis or human therapeutics is also presented. [less ▲]

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See detailBacillus-based biological control of plant diseases
Cawoy, Hélène ULg; Bettiol, Wagner; Fickers, Patrick ULg et al

in Stoytcheva, Margarita (Ed.) Pesticides in the Modern World - Pesticides Use and Management (2011)

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See detailAntilisterial Activity on Poultry Meat of Amylolysin, a Bacteriocin from Bacillus amyloliquefaciens GA1
Halimi, Badre Eddine ULg; Dortu, Carine; Arguelles Arias, Anthony ULg et al

in Probiotics and Antimicrobial Proteins (2010)

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