References of "Farnir, Frédéric"
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See detailEffect of storage and cooking on the fatty acid profile of omega-3 enriched eggs and pork meat marketed in Belgium
Douny, Caroline ULg; El Khoury, Rawad; Delmelle, Julien et al

in Food Science & Nutrition (2015), 3(2), 140-152

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See detailRational Development of an Attenuated Recombinant Cyprinid Herpesvirus 3 Vaccine Using Prokaryotic Mutagenesis and In Vivo Bioluminescent Imaging
Boutier, Maxime ULg; Ronsmans, Maygane ULg; Ouyang, Ping et al

in PLoS Pathogens (2015), 11(2), 1004690

Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We ... [more ▼]

Cyprinid herpesvirus 3 (CyHV 3) is causing severe economic losses worldwide in common and koi carp industries, and a safe and efficacious attenuated vaccine compatible with mass vaccination is needed. We produced single deleted recombinants using prokaryotic mutagenesis. When producing a recombinant lacking open reading frame 134 (ORF134), we unexpectedly obtained a clone with additional deletion of ORF56 and ORF57. This triple deleted recombinant replicated efficiently in vitro and expressed an in vivo safety/efficacy profile compatible with use as an attenuated vaccine. To determine the role of the double ORF56-57 deletion in the phenotype and to improve further the quality of the vaccine candidate, a series of deleted recombinants was produced and tested in vivo. These experiments led to the selection of a double deleted recombinant lacking ORF56 and ORF57 as a vaccine candidate. The safety and efficacy of this strain were studied using an in vivo bioluminescent imaging system (IVIS), qPCR, and histopathological examination, which demonstrated that it enters fish via skin infection similar to the wild type strain. However, compared to the parental wild type strain, the vaccine candidate replicated at lower levels and spread less efficiently to secondary sites of infection. Transmission experiments allowing water contamination with or without additional physical contact between fish demonstrated that the vaccine candidate has a reduced ability to spread from vaccinated fish to naïve sentinel cohabitants. Finally, IVIS analyses demonstrated that the vaccine candidate induces a protective mucosal immune response at the portal of entry. Thus, the present study is the first to report the rational development of a recombinant attenuated vaccine against CyHV 3 for mass vaccination of carp. We also demonstrated the relevance of the CyHV 3 carp model for studying alloherpesvirus transmission and mucosal immunity in teleost skin. [less ▲]

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See detailFunctional analysis of the three HMA4 copies of the metal hyperaccumulator Arabidopsis halleri
Nouet, Cécile ULg; Charlier, Jean-Benoit; Carnol, Monique ULg et al

in Journal of Experimental Botany (2015), in press

In Arabidopsis halleri, the AhHMA4 gene has an essential function in Zn/Cd hypertolerance and hyperaccumulation by mediating root to shoot translocation of metals. Constitutive high expression of AhHMA4 ... [more ▼]

In Arabidopsis halleri, the AhHMA4 gene has an essential function in Zn/Cd hypertolerance and hyperaccumulation by mediating root to shoot translocation of metals. Constitutive high expression of AhHMA4 results from a tandem triplication and cis-activation of the promoter of all three copies. The three AhHMA4 copies possess divergent promoter sequences, but highly conserved coding sequences, and display identical expression profiles in the root and shoot vascular system. Here, we expressed an AhHMA4::GFP fusion under the control of each three A. halleri HMA4 promoters in a hma2hma4 double mutant of Arabidopsis thaliana to individually examine the function of each A. halleri AhHMA4 copy. The protein localized non-polarly at the plasma membrane of the root pericycle cells of both A. thaliana and A. halleri. The expression of each AhHMA4::GFP copy complemented the severe Zn deficiency phenotype of the hma2hma4 mutant by restoring root-to-shoot translocation of zinc. However, each copy had different impact on metal homeostasis in the A. thaliana genetic background: AhHMA4 copies 2 and 3 were more highly expressed and provided higher Zn tolerance in roots and accumulation in shoots than copy 1, whereas AhHMA4 copy 3 also increased Cd tolerance in roots. Our data suggest a certain extent of functional differentiation among the three A. halleri HMA4 copies, stemming from differences in expression levels rather than in expression profile. HMA4 is a key node of the Zn homeostasis network and small changes in expression level can have major impact on Zn allocation to root or shoot tissues. [less ▲]

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See detailInfluence of Morphine on the Rabbit Gastrointestinal Tract
Deflers, Hélène ULg; Bolen, Géraldine ULg; Gandar, Frederic ULg et al

Conference (2014, October 20)

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See detailAssessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis
Mercier; Peters, Iain; Farnir, Frédéric ULg et al

in BMC Veterinary Research (2014)

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See detailSelective breeding of Arabian and Thoroughbred racehorses in Algeria: perceptions, objectives and practices of owners-breeders
Tennah, Safia ULg; Farnir, Frédéric ULg; Kafidi, Nacerredine et al

in Revista Brasileira de Zootecnia (2014), 43(4), 188-196

This survey, conducted with 461 racehorse owners-breeders in Algeria between 2009 and 2011, investigates their perceptions, objectives and practices regarding selective breeding. Racehorse breeding is a ... [more ▼]

This survey, conducted with 461 racehorse owners-breeders in Algeria between 2009 and 2011, investigates their perceptions, objectives and practices regarding selective breeding. Racehorse breeding is a full-time professional activity for a third of interviewees. The holdings are small-sized with 77% owning one or two mares. The regular practice of mating is here used to categorize breeders according to their degree of professionalization (38.4% professional vs. 61.6% occasional breeders). Experience in the sector was also used to classify breeders, considering as “junior” the breeders under 10 years experience (38.8%) and as “senior” those above 10 years (61.2%). More than professionalization, experience shows a significant impact on practices and objectives. Thus, experience influences breed choice (junior breeders tend to specialize while senior own both Arabian and Thoroughbreds), age at first foaling (sooner among senior breeders), information sources considered for selecting stallions (senior use more diversified sources), the importance granted to the price of mating (greater for junior breeders), the importance granted to the ranking compared to earnings (the ranking being more important to junior breeders), and the priority given to breeding (junior breeders give higher priority to a buy-race-resell activity). Finally, racehorse breeding is poorly professionalized, the only financial goal being cost coverage. Despite inappropriate practices, an interest for selection is noticed. [less ▲]

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See detailUtilization of Large White x Mong Cai crossbred sows, Duroc and stress negative Piétrain boars for the production of fattening pigs under household conditions in northern Vietnam
Do Duc, Luc ULg; Clinquart, Antoine ULg; Vu Dinh, Ton et al

in Animal Production Science (2014)

The objective of the present study was to compare Duroc (DU) and stress-negative Piétrain (PI) as terminal sires mated to F1(Large White x Mong Cai) sows (F1) on productivity, carcass characteristics ... [more ▼]

The objective of the present study was to compare Duroc (DU) and stress-negative Piétrain (PI) as terminal sires mated to F1(Large White x Mong Cai) sows (F1) on productivity, carcass characteristics, meat quality and meat chemical composition under household conditions in Hai Duong province, Vietnam. The reproduction performances were based on 74 litters of 42 F1 sows from 12 households and the semen from 11 Duroc and 10 Piétrain boars. Data on production and carcass traits were collected from 76 DUxF1and 67 PIxF1 crossbred fattening pigs. Longissimus dorsi samples from 55 and 37 of DUxF1 and PIxF1 respectively were analyzed to determine meat quality and meat chemical composition. The results show that household affected production, carcass, and meat quality traits (P<0.05) as well as reproduction (weights at birth and weaning, P<0.01). Inversely, boar breeds did not influence (P>0.06) on reproduction traits (except individual weight of born alive pigs and weaned pigs, P<0.01), production traits, meat chemical composition and meat quality (except pH at 4 days post mortem, P<0.05) while lean meat percentage of Piétrain-sired pigs was higher than those of Duroc-sired pigs (P<0.05). The utilization of Piétrain boars does not affect reproduction performances, growth rate and meat quality while improving lean meat percentage in comparison to Duroc boars. This suggests that the use of stress-negative Piétrain boars as terminal sires would improve lean meat percentage under household conditions in North Vietnam. [less ▲]

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See detailTraining Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages
Frellstedt, Linda ULg; Waldschmidt, Ingrid; Gosset, Philippe et al

in American Journal of Respiratory Cell and Molecular Biology (2014), 51(1), 135-142

In humans, strenuous exercise causes increased susceptibility to respiratory infections associated with down-regulated expression of Toll-like receptors (TLRs), co-stimulatory and antigen-presenting ... [more ▼]

In humans, strenuous exercise causes increased susceptibility to respiratory infections associated with down-regulated expression of Toll-like receptors (TLRs), co-stimulatory and antigen-presenting molecules. Lower airway diseases are also a common problem in sport and racing horses. Because the innate immunity plays an essential role in lung defense mechanisms, we aimed to assess the effect of acute exercise and training on innate immune responses in two different compartments. Blood monocytes and pulmonary alveolar macrophages (PAM) were collected from horses in an untrained, moderately and intensively trained as well as deconditioned state before and after a strenuous exercise test (SET). The cells were analysed for TLR mRNA expression by real-time PCR in vitro and the cytokine production after in vitro stimulation with TLR ligands was measured by ELISA. Our results showed that training, but not acute exercise, modified the innate immune responses in both compartments. The mRNA expression of TLR3 was down-regulated by training in both cell types, whereas the expression of TLR4 was up-regulated in monocytes. Monocytes treated with lipopolysaccharide (LPS) and a synthetic diacylated lipoprotein (FSL) showed increased cytokine secretion in trained and deconditioned subjects indicating the activation of cells at the systemic level. The production of TNF-alpha and IFN-beta in non-stimulated and stimulated PAM was decreased in trained and deconditioned horses and might therefore explain the increased susceptibility to respiratory infections. Our study reports a dissociation between the systemic and the lung response to training that is probably implicated in the systemic inflammation and in the pulmonary susceptibility to infection. [less ▲]

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See detailL’élevage caprin dans la région montagneuse d’Ath Waghlis dans la wilaya de Bejaia: typologie et caractérisation morpho-biométrique
Moula, Nassim ULg; Ait Kaki, Asma ULg; Touazi, Leghel et al

Poster (2013, November 30)

Le cheptel caprin algérien est estimé à 3,8 millions de têtes dont 2,2 millions de femelles adultes. Comptant 400000 têtes, la race caprine kabyle représente un dixième du cheptel national. Afin de ... [more ▼]

Le cheptel caprin algérien est estimé à 3,8 millions de têtes dont 2,2 millions de femelles adultes. Comptant 400000 têtes, la race caprine kabyle représente un dixième du cheptel national. Afin de caractériser cette race et son élevage dans la région d’Ath Waghlis, une enquête a été menée auprès de 69 éleveurs (dont 22 femmes) de chèvres répartis sur les communes d’Akfadou, Chemini, Souk Oufella et Tibane. Les questionnaires portaient sur le profil socio-économique du ménage et ses activités agricoles, ainsi que les données techniques se rapportant à la production de chèvres. Il a également été procédé à la caractérisation morpho-biométrique des chèvres (18 mensurations corporelles, 3 variables qualitatives). L’analyse des structures d’élevage par classification hiérarchique ascendante (CHA) a permis de retenir quatre groupes d’élevages conservant 55,7% de la variabilité totale. Les variables considérées étaient le nombre d’animaux par espèce (chèvre, bovin, ovin, lapin, poule, dinde, ruches) et le nombre d’arbres (oliviers et figuiers) détenus. Le nombre moyen de chèvres pour chaque groupe est de 7,2±2,8, 11,1±3,5, 22,3±1,4 et 3,4±1,0. Le groupe 3, démontrant les plus grands effectifs caprins, est composé de personnes âgées en moyenne de 67 ans et ne pratiquant pas l’arboriculture. Ils possèdent également les plus importants effectifs d’ovins (48,67), de lapins (50,83), de poules (48,33) et de dindes (42). Le groupe 4, qui a les effectifs caprins les plus faibles, représente le groupe des jeunes éleveurs (moyenne ~39 ans), orientés vers l’élevage bovin (~52 têtes) et la production d’oliviers (~207 oliviers) et de figuiers (~47 figuiers). Les 18 variables morpho-biométriques étaient significativement plus élevées chez les mâles que chez les femelles (p<0,05). La chèvre kabyle est de petite taille (mâle : 68,23 cm vs femelle : 65,41 cm) et à poil long (mâle : 12 cm vs femelle 9 cm). Ses oreilles sont tombantes, le profil convexe à cassure nasale peu accentuée et sa robe va du brun foncé au noir. La riche diversité génétique des caprins locaux à Ath Waghlis souffre du manque de coordination des efforts de sélection des éleveurs, notamment du fait de l’absence de standard de la race et par l’introduction de races exotiques. Cette richesse devrait représenter un vivier utile où puiser les gènes d’intérêt pour la valorisation de la production locale, basée sur un engagement fort des éleveurs dans la production d’individus correspondant à un idéal collectivement déterminé. [less ▲]

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See detailLes ressources génétiques ovines en Algérie
Moula, Nassim ULg; Tennah, Safia ULg; Philippe, François-Xavier ULg et al

Poster (2013, November 30)

Avec un cheptel avoisinant les 19 millions de têtes, l'élevage ovin occupe une place importante en Algérie. Outre sa contribution de plus de 50 % dans la production nationale de viandes rouges et de 10 à ... [more ▼]

Avec un cheptel avoisinant les 19 millions de têtes, l'élevage ovin occupe une place importante en Algérie. Outre sa contribution de plus de 50 % dans la production nationale de viandes rouges et de 10 à 15% dans le produit intérieur brut agricole, l’élevage ovin joue un rôle socioculturel important. Il se pratique dans toutes les zones climatiques, depuis la côte méditerranéenne jusqu'aux oasis du grand Sahara. Cette diversité pédoclimatique du plus grand pays africain offre à l’Algérie une extraordinaire diversité de races ovines, avec huit races caractérisées par une rusticité remarquable, adaptées à leurs milieux respectifs. Avec 63% du cheptel ovin total, la race Ouled Djellal, aussi appelée la race Blanche, est la plus importante race ovine algérienne. Elle est exploitée principalement pour la production de viande. La race Berbère représentant un quart du cheptel ovin national, est considérée comme la plus ancienne race algérienne. Elle est menacée par les croisements non-contrôlés avec d’autres races pour l’amélioration de son potentiel productif. La troisième race ovine très importante est la Rmbi avec 11,1% du cheptel national, considérée comme la plus lourde race ovine en Algérie avec des poids avoisinants les 90 kg chez le bélier et 60kg chez la brebis. Les races Hamra, Barbarine, D'man, Sidahou et Tazegzawt représentent ensemble moins de 1% du cheptel ovin algérien. Le déclin de ces populations illustre l’érosion dramatique que subit cette richesse exceptionnelle, appelant à la mise en place d’un plan national de gestion et de conservation des ressources génétiques. [less ▲]

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