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See detailHoneydew volatile emission acts as a kairomonal message for the Asian lady beetle Harmonia axyridis (Coleoptera: Coccinellidae)
Leroy, Pascal; Heuskin, Stéphanie ULg; Sabri, Ahmed ULg et al

in Insect Science (2012), 19(4), 498-506

The Asian lady beetle Harmonia axyridis Pallas is considered as an invasive species in most territories where it has been introduced. Because aphid honeydew acts as an attractant for many aphid predators ... [more ▼]

The Asian lady beetle Harmonia axyridis Pallas is considered as an invasive species in most territories where it has been introduced. Because aphid honeydew acts as an attractant for many aphid predators and parasitoids, the objectives of this work were to collect and identify the volatile compounds released from the aphid excretory product to evaluate how these semiochemicals could affect the H. axyridis foraging behavior. Twelve volatile chemicals were identified from the Megoura viciae Buckton honeydew including four alcohols, three ketones, three aldehydes, a pyrazine and a monoterpene. The volatiles 3-methyl-1-butanol and 3-methyl-butanal were highlighted as the two most abundant semiochemicals released from the M. viciae honeydew. Vicia faba L. plants treated with crude honeydew attracted more than 80% of the tested individuals with 40% of attracted beetles located on the plant. Four volatile compounds (3-hydroxy-2-butanone, 3-methyl-butanal, 3-methyl-1-butanol and limonene) were also highlighted to attract more than 75% of the coccinellids toward the odor source and to locate about 35% of them on the plants. Limonenewas the most efficient attractant since 89% of the H. axyridis responded to this odor. The use of the identified semiochemicals aswell as the composition of an artificial honeydew could certainly be helpful to control the dispersal of the Asian lady beetle H. axyridis. [less ▲]

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See detailValidation of a fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Rozet, Eric; Lorge, Stéphanie et al

in Journal of Pharmaceutical & Biomedical Analysis (2010), 53

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management ... [more ▼]

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management approach is described. Accuracy profiles using total error as decision criteria for validation were used to verify the overall accuracy of the method results within a well defined range of concentrations and to determine the lowest limit of quantification for each analyte. Furthermore it allowed to select a very simple and reliable regression model for calibration curve for the quantification of both analytes as well as to provide measurement uncertainty without any additional experiments. Finally, this validated method was used for the quantification of semiochemicals in slow release formulations. The goal was to verify the protection efficiency of alginate gel beads formulations against oxidation and degradation of sesquiterpenes. The results showed that the alginate beads are adequate slow release devices which protect the bio-active molecules during at least twenty days. [less ▲]

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See detailValidation of a fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Rozet, Eric; Lorge, Stéphanie et al

in Journal of Pharmaceutical & Biomedical Analysis (2010), 53

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management ... [more ▼]

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management approach is described. Accuracy profiles using total error as decision criteria for validation were used to verify the overall accuracy of the method results within a well defined range of concentrations and to determine the lowest limit of quantification for each analyte. Furthermore it allowed to select a very simple and reliable regression model for calibration curve for the quantification of both analytes as well as to provide measurement uncertainty without any additional experiments. Finally, this validated method was used for the quantification of semiochemicals in slow release formulations. The goal was to verify the protection efficiency of alginate gel beads formulations against oxidation and degradation of sesquiterpenes. The results showed that the alginate beads are adequate slow release devices which protect the bio-active molecules during at least twenty days. [less ▲]

Detailed reference viewed: 101 (28 ULg)
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See detailValidation of a fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Rozet, Eric; Lorge, Stéphanie et al

in Journal of Pharmaceutical & Biomedical Analysis (2010), 53

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management ... [more ▼]

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management approach is described. Accuracy profiles using total error as decision criteria for validation were used to verify the overall accuracy of the method results within a well defined range of concentrations and to determine the lowest limit of quantification for each analyte. Furthermore it allowed to select a very simple and reliable regression model for calibration curve for the quantification of both analytes as well as to provide measurement uncertainty without any additional experiments. Finally, this validated method was used for the quantification of semiochemicals in slow release formulations. The goal was to verify the protection efficiency of alginate gel beads formulations against oxidation and degradation of sesquiterpenes. The results showed that the alginate beads are adequate slow release devices which protect the bio-active molecules during at least twenty days. [less ▲]

Detailed reference viewed: 101 (28 ULg)
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See detailValidation of a fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Rozet, Eric; Lorge, Stéphanie et al

in Journal of Pharmaceutical & Biomedical Analysis (2010), 53

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management ... [more ▼]

The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-β-farnesene and β-caryophyllene) to be used in an integrated pest management approach is described. Accuracy profiles using total error as decision criteria for validation were used to verify the overall accuracy of the method results within a well defined range of concentrations and to determine the lowest limit of quantification for each analyte. Furthermore it allowed to select a very simple and reliable regression model for calibration curve for the quantification of both analytes as well as to provide measurement uncertainty without any additional experiments. Finally, this validated method was used for the quantification of semiochemicals in slow release formulations. The goal was to verify the protection efficiency of alginate gel beads formulations against oxidation and degradation of sesquiterpenes. The results showed that the alginate beads are adequate slow release devices which protect the bio-active molecules during at least twenty days. [less ▲]

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See detailContribution to the study of semiochemical slow release formulations. Development of flash chromatographic methods.
Heuskin, Stéphanie ULg; Farmakidis, Julien; Lorge, Stéphanie et al

Poster (2009)

Detailed reference viewed: 21 (7 ULg)