References of "Faisca, Pedro"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailA Critical Appraisal of Carbon Monoxide Uptake Measurements for the Follow-up of Experimental Respiratory Diseases in the Laboratory Mouse
Habyarimana, Jean Adélite; Flandre, Thierry; Faisca, Pedro et al

in Scandinavian Journal of Laboratory Animal Science (2009), 36(3), 229-240

Adaptation of double-chamber plethysmography to the laboratory mouse was recently proven to yield stable and reliable pulmonary function values. This approach to investigation of the respiratory function ... [more ▼]

Adaptation of double-chamber plethysmography to the laboratory mouse was recently proven to yield stable and reliable pulmonary function values. This approach to investigation of the respiratory function in mice owes its success to its decisive advantages in terms of non-invasiveness, practical implementation and generation of quantitative flow/volume measurements and nondisputed airway resistance calculation. When implemented to screen the resistance/susceptibility patterns to pathogens displayed by a panel of mouse inbred strains, the resistance value obtained was indeed able to detect tracheobronchic inflammation and to scale its severity. However, extension of the pathological process to most distal parts of the respiratory system did not translate in further alteration of resistance, suggesting that its value rather reflects constraints acting on airflow in the airways than pathologic processes located in the more distal parts of the lungs. In this context, we hypothesized that a more exhaustive functional picture could be obtained, still noninvasively, by combining double-chamber plethysmography with carbon monoxide (CO) uptake measurements. The feasibility of CO-uptake measurements in mice was demonstrated and the conditions under which reproducibility can be maximized were defined. Differences linked to strain, somatic growth, and sex were examined and discussed, and reference values in growing male and female conscious and healthy BALB/cBy, SJL/J, C57BL/6, C3H/HeN, DBA/2 and 129/Sv mice were given. Finally, double-chamber plethysmography and CO-uptake values were proven to be exquisitely complementary in assessing and dissecting the functional impact of Sendai virus pneumonia in the laboratory mouse. [less ▲]

Detailed reference viewed: 47 (10 ULg)
Full Text
Peer Reviewed
See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma.
Henry, E.; Desmet, Christophe ULg; Garze, V. et al

in Journal of Immunology (2008), 181(10), 7230-7242

Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to ... [more ▼]

Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to express the immunosuppressive cytokine IL-10 and tested the ability of these cells to control experimental asthma. A single intratracheal injection of OVA-pulsed IL-10-transduced DCs (OVA-IL-10-DCs) to naive mice before OVA sensitization and challenge prevented all of the cardinal features of airway allergy, namely, eosinophilic airway inflammation, airway hyperreactivity, and production of mucus, Ag-specific Igs, and IL-4. OVA-IL-10-DCs also reversed established experimental asthma and had long-lasting and Ag-specific effects. We furthermore showed, by using IL-10-deficient mice, that host IL-10 is required for mediating the immunomodulatory effects of OVA-IL-10-DCs and demonstrated a significant increase in the percentage of OVA-specific CD4(+)CD25(+)Foxp3(+)IL-10(+) regulatory T cells in the mediastinal lymph nodes of OVA-IL-10-DC-injected mice. Finally, adoptive transfer of CD4(+) mediastinal lymph node T cells from mice injected with OVA-IL-10-DCs protected OVA-sensitized recipients from airway eosinophilia upon OVA provocation. Our study describes a promising strategy to induce long-lasting Ag-specific tolerance in airway allergy. [less ▲]

Detailed reference viewed: 65 (15 ULg)
Full Text
Peer Reviewed
See detailSendai virus, the mouse parainfluenza type 1: A longstanding pathogen that remains up-to-date
Faisca, Pedro; Desmecht, Daniel ULg

in Research in Veterinary Science (2007), 82(1), 115-125

Biologically speaking, Sendai virus (SeV), the murine parainfluenza virus type 1, is perceived as a common respiratory pathogen that is endemic in many rodent colonies throughout the world. Currently it ... [more ▼]

Biologically speaking, Sendai virus (SeV), the murine parainfluenza virus type 1, is perceived as a common respiratory pathogen that is endemic in many rodent colonies throughout the world. Currently it is believed that SeV is the leading cause of pneumonia in mice and together with the mouse hepatitis viruses, is the most prevalent and important of the naturally occurring infections of mice. The scientific community also considers SeV as the archetype organism of the Paramyxoviridae family because most of the basic biochemical, molecular and biologic properties of the whole family were derived from its own characteristics. Recently, scientific interest for this old pathogen has re-emerged, this time because of its potential value as a vector for gene transfer. This review aimed at drawing an exhaustive picture of this multifaceted pathogen. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailSuppression of pattern recognition receptor TLR4 sensing does not alter lung responses to pneumovirus infection
Faisca, Pedro; Bui Tran Anh, Dao; Thomas, Anne et al

in Microbes & Infection (2006), 8

Toll-like receptors (TLR) are an important component in the innate immune response to a wide variety of pathogens. Recently, a series of studies has addressed the hypothesis that TLR4 also participates in ... [more ▼]

Toll-like receptors (TLR) are an important component in the innate immune response to a wide variety of pathogens. Recently, a series of studies has addressed the hypothesis that TLR4 also participates in the host innate response against respiratory syncytial virus (RSV), the leading cause of lower respiratory tract infections in infants and young children. In most of the studies available, RSV, which is not a natural pathogen of mice, has been systematically used in mouse models of human bronchiolitis, with conflicting results. Pneumonia virus of mice (PVM), a member of the pneumovirus genus, shares many similarities with RSV. The serological and structural relationships that exist between them suggest that the immune response to these viruses may be similar in their respective natural hosts. To determine the role of TLR4 in host defense against PVM, TLR4-competent and TLR4-deficient mice were intranasally infected with PVM. Variation of body weight, pulmonary function values, histopathology, and pulmonary viral loads were analyzed. None of the investigated clinical, functional, histological and virological parameters was different between strains, which demonstrates that the sensitivity of the mouse to its natural pneumovirus infection is independent of the presence or absence of TLR4 sensing. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailSendai virus-induced alterations in lung structure/function correlate with viral loads and reveal a wide resistance/susceptibility spectrum among mouse strains
Faisca, Pedro; Bui Tran Anh, Dao; Desmecht, Daniel ULg

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2005), 289

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence ... [more ▼]

The Paramyxoviridae family includes some of the most important and ubiquitous disease-causing viruses of infants and children, most of which cause significant infections of the respiratory tract. Evidence is accumulating in humans that genetic factors are involved in the severity of clinical presentation. As a first step toward the identification of the genes involved, this study was undertaken to establish whether laboratory mouse strains differ in susceptibility to Sendai virus, the murine counterpart of human type-1 parainfluenza virus which, historically, has been used extensively in studies that have defined the basic biological properties of paramyxoviruses in general. With this purpose in mind, double-chamber plethysmography data were collected daily for 7 days after inoculation of Sendai virus in six inbred strains of mice. In parallel, histological examinations and lung viral titration were carried out from day 5 to day 7 after inoculation. Pulmonary structure/function values closely reflected the success of viral replication in the lungs and revealed a pattern of continuous variation with resistant, intermediate, and susceptible strains. The results unambiguously suggest that BALB/c (resistant) and 129Sv (susceptible) strains should be used in crossing experiments aimed at identifying the genes involved in resistance to Paramyxoviridae by the positional cloning approach. [less ▲]

Detailed reference viewed: 17 (0 ULg)