References of "Evers, Danièle"
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See detailAscorbic acid, Phenolic acid, Flavonoid and Carotenoid profiles of selected extracts from Ribes nigrum
Tabart, Jessica ULg; Kevers, Claire ULg; Evers, Danièle et al

in Journal of Agricultural and Food Chemistry (2011), 59

Small fruits such as berries have low energy contents, but high content of vitamins, micronutrients, and dietary fibres and constitute a good source of natural antioxidant compounds that are important ... [more ▼]

Small fruits such as berries have low energy contents, but high content of vitamins, micronutrients, and dietary fibres and constitute a good source of natural antioxidant compounds that are important constituents of the human diet. This study allowed to identify a large number of compounds in an extract of blackcurrant showing high antioxidant activity and to compare their profile in various parts of the plants (leaves, buds and berries). If it was known that berries contained very high levels of natural phenolic compounds, here we showed that leaves and buds could also be considered as a good sources of natural antioxidants. Indeed, they contained high amount of ascorbic acid, phenolic acids, flavonoids and carotenoids. An acetone mixture can extract several classes of phenolic compounds with a good yield of ascorbic acid, flavonols, flavan-3-ols and anthocyanins. For phenolic acids, it extracted fewer compounds than a specific extraction solution. [less ▲]

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See detailA late blight resistant potato plant overexpresses a gene coding for alpha-galactosidase upon infection by Phytophthora infestans
Evers, Danièle; Ghislain, Marc; Hoffmann, Lucien et al

in Biologia Plantarum (2006), 50(2), 265-271

Late blight of potato, caused by Phytophthora infestans was studied by using a resistant clone of potato on one side and a susceptible clone on the other side. A gene coding putatively for an alpha ... [more ▼]

Late blight of potato, caused by Phytophthora infestans was studied by using a resistant clone of potato on one side and a susceptible clone on the other side. A gene coding putatively for an alpha-galactosidase has been isolated by mRNA reverse transcription polymerase chain reaction differential display and was shown to be differentially expressed between the resistant and the susceptible clone. alpha-Galactosidases catalyse the hydrolysis of alpha-1,6 linked alpha-galactose residues from oligosaccharides and it could be shown in the present work that raffinose content decreases at 30 h after infection by P. infestans in the resistant clone. [less ▲]

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See detailTwo PR-1 loci detected in the native cultivated potato Solanum phureja appear differentially expressed upon challenge by late blight
Evers, Danièle; Schweitzer, C.; Nicot, N. et al

in Physiological and Molecular Plant Pathology (2006), 67(3-5, SEP-OCT), 155-163

Plant pathogenesis-related proteins are toxic to invading pathogens. Among them, the Subfamily PR-1 represents low-molecular weight proteins of unknown biochemical function. Here, we describe the cloning ... [more ▼]

Plant pathogenesis-related proteins are toxic to invading pathogens. Among them, the Subfamily PR-1 represents low-molecular weight proteins of unknown biochemical function. Here, we describe the cloning and isolation of two PR-1 genes (PR-1b1 (GenBank accession no. SPH493450) and PR-1b2 (SPH493451)) that encode predicted basic proteins. We isolated them from Solanum phureja, a native Andean potato with horizontal resistance to late blight, caused by the oomycete Phytoplithora infestans. We demonstrate that the PR-1 genes belong to a small multigene family with an estimated copy number of 4-6 with one of them located oil chromosome IX as determined by genetic mapping. The expression of PR-1 genes was different in late blight resistant and Susceptible genotypes. Therefore, we propose that both PR-1 genes may play a role in horizontal late blight resistance of S. phureja. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailDifferential gene expression in two potato lines differing in their resistance to Phytophthora infestans
Evers, Danièle; Ghislain, Marc; Hausman, Jean-François et al

in Journal of Plant Physiology (2003), 160(6), 709-712

Horizontal resistance to late blight in the potato is a primary objective of many breeding programs. Knowledge of the physiological and biochemical mechanisms underlying it, however, is scarce. The ... [more ▼]

Horizontal resistance to late blight in the potato is a primary objective of many breeding programs. Knowledge of the physiological and biochemical mechanisms underlying it, however, is scarce. The purpose of the present study was the identification of these physiological and biochemical factors in plant material obtained by crossing a late blight resistant Solanum phureja clone with a susceptible dihaploid of S. tuberosum subsp. tuberosum. The mRNA RT-PCR differential display method was used to compare the gene expression patterns of a resistant hybrid with that of a susceptible one. By sequence homology, we identified several genes with diverse functions, including genes known to be involved in resistance or stress responses and genes known to be involved in primary or secondary metabolism. [less ▲]

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See detailCarbohydrates and resistance to Phytophthora infestans in potato plants
Evers, Danièle; Dommes, Jacques ULg; Hausman, Jean-François

in Acta Physiologiae Plantarum (2003), 25(2), 171-178

Plants generally deal with biotic or abiotic stresses by altering components as for example cell wall constituents and metabolites. Infection by Phytophthora infestans, the causal agent of late blight ... [more ▼]

Plants generally deal with biotic or abiotic stresses by altering components as for example cell wall constituents and metabolites. Infection by Phytophthora infestans, the causal agent of late blight, constitutes a stress condition for the plants and they react to it with changes arising in their metabolism depending on the resistance level of the plants. The present work compares two potato hybrids differing in their level of horizontal resistance to late blight. Carbohydrate content in stems and leaves of infected and uninfected plants was determined by HPLC. Some carbohydrates accumulated in the stems of the resistant hybrid infected by P. infestans, whereas they remained unchanged in the susceptible hybrid. On the other hand, in the leaves, these carbohydrates accumulated only in the infected susceptible hybrid. [less ▲]

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