References of "El Allaf, M"
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See detailFast determination of myoglobin in serum using a new radial partition immunoassay
Chapelle, Jean-Paul ULg; Lemache, K.; el Allaf, M. et al

in Clinical Biochemistry (1994), 27(5), 423-8

Detailed reference viewed: 21 (3 ULg)
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See detailQuantitative turbidimetric assay for determining myoglobin evaluated.
Delanghe, J.; Chapelle, Jean-Paul ULg; el Allaf, M. et al

in Annals of Clinical Biochemistry (1991), 28 ( Pt 5)

A recently introduced turbidimetric immunoassay using shell/core particles for determination of myoglobin (Turbiquant Myoglobin, Behringwerke, Marburg, Germany) was evaluated on the Behring Turbitimer ... [more ▼]

A recently introduced turbidimetric immunoassay using shell/core particles for determination of myoglobin (Turbiquant Myoglobin, Behringwerke, Marburg, Germany) was evaluated on the Behring Turbitimer analyzer. Intra-assay CV varied between 1.0% and 3.2%; interassay CV was between 2.0% and 3.6%. Linear results were obtained between 2.8 nmol/L and 36.7 nmol/L. Manual dilution in saline solution allowed measurement of myoglobin concentrations up to 25,400 nmol/L. In the tested range, no high-dose 'hook' effect was observed. Haemolysis interfered with the assay when haemoglobin concentrations exceeded 2 g/L. The occurrence of error codes due to the presence of triglycerides was shown to depend on particle size. Repeat assays on diluted samples were necessary at triglyceride concentrations of 2.3 mmol/L for post-prandial chylomicrons (200-1000 nm), and at 11-31 mmol/L for very-low density lipoprotein-triglycerides (80-200 nm). No significant interferences of haptoglobin, bilirubin, iodine containing contrast media, and rheumatoid factors were detected for the assay. Treatment of lipaemic samples with Lipoclean (Behringwerke) resulted in lower myoglobin values. Simultaneously drawn serum and heparin-plasma samples gave comparable myoglobin results. However, values obtained in EDTA- or citrate-treated plasma samples occasionally differed significantly from serum values. The upper reference limit for myoglobin concentration was 4.2 nmol/L. The turbidimetric assay correlated well with a radioimmunoassay (Byk-Sangtec; r = 0.892) and with the automated nephelometric assay (Behringwerke; r = 0.944). Values obtained by turbidimetry were comparable to those obtained by the latex agglutination test (Behringwerke). [less ▲]

Detailed reference viewed: 47 (0 ULg)
See detailAnalytical and clinical performances of a new automated immunoassay for measuring CK-MB in serum
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1990, October)

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See detailUsefulness of CRP determination after acute myocardial infarction
Chapelle, Jean-Paul ULg; El Allaf, M.; Pierard, Luc ULg et al

Poster (1990, July)

Detailed reference viewed: 5 (1 ULg)
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See detailDetermination of myoglobin in serum by kinetic turbidimetry, using the turbitimesystem
Chapelle, Jean-Paul ULg; El Allaf, M.

in Clinical Chemistry (1990), 36(6), 1193

Detailed reference viewed: 4 (0 ULg)
See detailRadial partition immunoassay applied to automated quantification of CK-MB in serum
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1990, April)

Detailed reference viewed: 7 (0 ULg)
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See detailAutomated quantification of creatinine kinase MB isoenzyme in serum by radial partition immunoassay, with use of the Stratus analyzer
Chapelle, Jean-Paul ULg; El Allaf, M.

in Clinical Chemistry (1990), 36(1), 99-101

We evaluated the analytical and clinical performances of a new radial partition immunoassay for measuring the mass concentration of creatine kinase (CK)-MB in serum. All pipetting, washes, incubations and ... [more ▼]

We evaluated the analytical and clinical performances of a new radial partition immunoassay for measuring the mass concentration of creatine kinase (CK)-MB in serum. All pipetting, washes, incubations and data reduction were performed in 8 min by the Stratus (Dade) fluorometric analyzer. Within-assay and between-assay CVs were respectively 5.5% and 8.4% at 21 micrograms/L, and 4.2% and 3.4% at 48 micrograms/L. Assaying serial dilutions of serum samples with high CK-MB concentrations demonstrated excellent linearity. Results of the Stratus technique correlated well (n = 115, r = 0.98) with those of the Tandem-E CKMB II assay. There was no interference from hemolysis, bilirubin, rheumatoid factor, or added CK-MM (up to 3500 U/L); consequently, CK-MB can be determined in undiluted serum, even in the presence of high total CK activity. The mean CK-MB concentration in 105 blood donors was 1.9 (SD 1.3) micrograms/L. For seven myocardial infarction patients who received prompt fibrinolytic therapy, the mean CK-MB concentration was 4.5 (SD 1.8) micrograms/L at admission, and maximum concentrations, 119 (SD 94) micrograms/L, were recorded 16 h later. CK-MB returned to concentrations less than 10 micrograms/L within 72 h. [less ▲]

Detailed reference viewed: 11 (3 ULg)
Peer Reviewed
See detailUsefulness of CRP determination after acute myocardial infarction
Chapelle, Jean-Paul ULg; El Allaf, M.; Pierard, Luc ULg et al

in Clinical Chemistry (1990), 36

Detailed reference viewed: 5 (0 ULg)
See detailSignificance of serum creatine kinase isoforms for assessing myocardial infarction
El Allaf, M.; Chapelle, Jean-Paul ULg; El Allaf, Dia ULg et al

Poster (1989, September)

Detailed reference viewed: 5 (1 ULg)
See detailAutomated quantification of creatine kinase-MB in serum by radial partition immunoassay
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1989, August)

Detailed reference viewed: 2 (0 ULg)
See detailAutomated quantification of creatine kinase (CK)-MB by a new fluorometric enzyme immunoassay
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1989, June)

Detailed reference viewed: 3 (0 ULg)