References of "El Allaf, Dia"
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See detailFast determination of myoglobin in serum using a new radial partition immunoassay
Chapelle, Jean-Paul ULg; Lemache, K.; el Allaf, M. et al

in Clinical Biochemistry (1994), 27(5), 423-8

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See detailEarly diagnosis of myocardial infarction using a new automated immunoassay for serum myoglobin
Chapelle, Jean-Paul ULg; El Allaf, Dia ULg

in European Heart Journal Supplements : Journal of the European Society of Cardiology (1993), 14(Abs. suppl.), 32

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See detailSignificance of serum creatine kinase isoforms for assessing myocardial infarction
El Allaf, M.; Chapelle, Jean-Paul ULg; El Allaf, Dia ULg et al

Poster (1989, September)

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See detailSignificance of serum creatine kinase isoforms for assessing myocardial infarction
El Allaf, M.; Chapelle, Jean-Paul ULg; El Allaf, Dia ULg et al

in European Heart Journal Supplements : Journal of the European Society of Cardiology (1989), 10(suppl.1), 224

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See detailDifferentiating muscle damage from myocardial injury by meaans of the serum creatinine kinase (CK) isoenzyme MB mass measurement/total CK activity ratio
el Allaf, M.; Chapelle, Jean-Paul ULg; El Allaf, Dia ULg et al

in Clinical Chemistry (1986), 32(2), 291-5

We immunoenzymometrically measured creatine kinase (CK) isoenzyme MB in extracts of myocardium and in homogenates of five different skeletal muscles. CK-MB concentrations in the former averaged 80.9 ... [more ▼]

We immunoenzymometrically measured creatine kinase (CK) isoenzyme MB in extracts of myocardium and in homogenates of five different skeletal muscles. CK-MB concentrations in the former averaged 80.9 micrograms/g wet tissue; in the skeletal muscles it varied widely, being (e.g.) 25-fold greater in diaphragm than in psoas. CK-MB in skeletal muscles ranged from 0.9 to 44 ng/U of total CK; the mean for myocardium was 202 ng/U. In sera from 10 trauma and 36 burn patients without myocardial involvement, maximum ratios for CK-MB mass/total CK activity averaged 7 (SEM 1) ng/U and 18 (SEM 6) ng/U, respectively. Except for an infant (220 ng/U), the highest ratio we found for serum after muscular damage was 38 ng/U. In contrast, the mean maximum ratio determined in 23 cases of acute myocardial infarction exceeded 200 ng/U. Among seven determinations performed 8 to 32 h after onset of symptoms, each infarct patient demonstrated at least one ratio greater than or equal to 110 ng/U. Ratios observed after infarct were unrelated to treatment received during the acute phase. We propose a CK-MB/total CK ratio of 80 ng/U as the cutoff value for differentiating myocardial necrosis from muscular injury. [less ▲]

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See detailRole de l' alpha 1-glycoproteine acide plasmatique dans la fixation de l'aminopyrine.
El Allaf, Dia ULg; Demey-Ponsart, E.; Pirotte, J. et al

in Archives Internationales de Physiologie et de Biochimie (1986), 94(5), 317-22

Under certain pathological conditions the binding of various substances by serum proteins is altered. The plasma concentrations of alpha 1-acid glycoprotein, also known as orosomucoid are reported to be ... [more ▼]

Under certain pathological conditions the binding of various substances by serum proteins is altered. The plasma concentrations of alpha 1-acid glycoprotein, also known as orosomucoid are reported to be elevated in stressful situation and in certain disease states like acute myocardial infarction. The binding of aminopyrine to serum proteins and alpha 1-acid glycoprotein was determined using equilibrium dialysis. It appears that alpha 1-acid glycoprotein has specific binding sites for aminopyrine. Aminopyrine was also bound to other serum proteins. On addition the results indicate that an increase of alpha 1-acid glycoprotein to concentrations such as those seen in some pathological states does not really alter the percent of aminopyrine bind to serum proteins. [less ▲]

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See detailSerum creatine kinase isoenzyme MB concentration after endomyocardial biopsy.
Chapelle, Jean-Paul ULg; El Allaf, Dia ULg; el Allaf, M. et al

in Clinica Chimica Acta (1986), 157(1), 55-63

Serum total creatine kinase (CK), CK-MB and myoglobin (Mb) were serially determined in 17 patients who underwent endomyocardial biopsy. Mean total CK levels increased from 36 +/- 27 U/l 30 min before ... [more ▼]

Serum total creatine kinase (CK), CK-MB and myoglobin (Mb) were serially determined in 17 patients who underwent endomyocardial biopsy. Mean total CK levels increased from 36 +/- 27 U/l 30 min before biopsy to a maximum of 112 +/- 77 U/l 8 h following the procedure (p less than 0.05). Similarly, Mb concentrations rose from 57 +/- 55 micrograms/l to 119 +/- 57 micrograms/l 30 min after biopsy (p less than 0.05). Normalization of total CK and Mb levels occurred within 16 and 8 h, respectively. A new immunoenzymetric assay (IEMA) was used to measure the mass concentration of the CK-MB molecule. The initial CK-MB levels were 0.2 +/- 0.4 microgram/l; a small but significant elevation was recorded as early as 2 h after biopsy (1.6 +/- 1.5 micrograms/l, p less than 0.05). CK-MB returned to initial concentration 16 h after the beginning of the procedure. Comparison with the maximum CK-MB levels recorded in 16 myocardial infarction patients (258 +/- 172 micrograms/l, range 90-680 micrograms/l) indicated that the modest increase of CK-MB level detected after biopsy probably reflects a limited endomyocardium lesion at the sampling site, excluding any significant myocardial damage. Total CK and Mb, which showed more pronounced elevations than CK-MB, are likely to originate from other sources than the myocardium. [less ▲]

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