References of "Duprez, Jean-Noël"
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See detailA 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger
Issa Ibrahim, Abdoulkarim; Duprez, Jean-Noël ULg; Bada-alambdeji, Rianatou et al

in Tropical Animal Health and Production (in press)

bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental ... [more ▼]

bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin- and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied [less ▲]

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See detailIdentification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC
Fakih, Ibrahim; Thiry, Damien ULg; Duprez, Jean-Noël ULg et al

in Veterinary Microbiology (2016)

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC ... [more ▼]

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www.genomicepidemiology.org). Of 233 isolates, 206 were triplex PCR-positive: 119 AE-STEC (58%), 78 EPEC (38%) and 9 STEC (4%); and the stx1+eae+ AE-STEC (49.5%) were the most frequent. Of them, 120 isolates (84% of AE-STEC, 23% of EPEC, 22% of STEC) tested positive with one O serogroup PCR: 57 for O26 (47.5%), 36 for O111 (30%), 10 for O103 (8%) and 8 for O5 (7%) serogroups. The analysis of the draft sequences of 15 O5 AE-STEC could not identify any difference correlated to the host. As a conclusion, (i) the AE-STEC associated with diarrhoea in young calves still belong to the same serogroups as previously (O5, O26, O111) but the O103 serogroup may be emerging, (ii) the O5 AE-STEC from calves and humans are genetically similar [less ▲]

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See detailEfficacy of Antisepsis Measures during Manual Milking on the Prevalence of Bacterial Mastitis at the Sahelian Experimental Station of Toukounous (Niger).
Issa Ibrahim, Abdoulkarim ULg; Bada-Alambedji, Rianatou; Duprez, Jean-Noël ULg et al

in African Journal of Microbiology Research (2015), 9(6), 348-354

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See detailAntibiotic resistance trend of Staphylococcus aureus isolated between 2010 and 2012 from mastitis cases in Azawak zebu in Niger
Issa Ibrahim, Abdoulkarim ULg; Duprez, Jean-Noël ULg; Bada-Alambedji, Rianatou et al

in African Journal of Microbiology Research (2014), 8((35)), 3271-3275

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See detailStructural esuation models to estimate risk infection and tolerance to bovine mastitis
Detilleux, Johann ULg; Theron, Léonard ULg; Duprez, Jean-Noël ULg et al

in Genetics, Selection, Evolution (2013), 45

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See detailGenotypic and phenotypic charactérisation of methicilin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis
Bardiau, M; Yamazaki, K; Duprez, Jean-Noël ULg et al

in Letters in Applied Microbiology (2013)

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See detailBacterial mastitis in the Azawak zebu breed at the Sahelian experimental station in Toukounous (Niger) : Identification and typing of Staphylococcus aureus
Issa, Ibrahim Abdoulkarim; Bada-Alambedji, Rianatou; Duprez, Jean-Noël ULg et al

in International Research Journal of Microbiology (2013), 4

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See detailIdentification and typing of Salmonella serotypes isolated from guinea fowl (Numida meleagris) farms in Benin during four laying seasons (2007-2010)
Boko, C; Kpodekon, T; Duprez, Jean-Noël ULg et al

in Avian Pathology : Journal of the W.V.P.A (2013), 42

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See detailEnterotoxaemia-like syndrome and Clostridium perfringens in veal calves
Muylaert, Adeline ULg; Lebrun, M.; Duprez, Jean-Noël ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2010), 167

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