  Thyroid-specific inactivation of Kif3a alters TSH signaling pathway and leads to hypothyroidism.; ; et al in Journal of Molecular Endocrinology (2013), 50 Kinesins, including the kinesin 2/KIF3 molecular motor, play an important role in intracellular traffic and can deliver vesicles to distal axon terminal, to cilia, to non-polarized cell surface or to ... [more ▼] Kinesins, including the kinesin 2/KIF3 molecular motor, play an important role in intracellular traffic and can deliver vesicles to distal axon terminal, to cilia, to non-polarized cell surface or to epithelial cell basolateral membrane, thus taking part to the establishment of cellular polarity. We report here the consequences of the kinesin 2 motor inactivation in the thyroid of 3 week-old Kif3a∆/flox Pax8Cre/+ mutant mice. Our results indicate first that 3 week-old Pax8Cre/+ mice used in these experiments present minor thyroid functional defects resulting in a slight increase in circulating bioactive TSH and intracellular cAMP levels, sufficient to maintain blood T4 levels in the normal range. Second, Kif3a inactivation in thyrocytes markedly amplified the phenotype observed in Pax8Cre/+ mice, resulting in an altered TSH signaling upstream of the second messenger cAMP and mild hypothyroidism. Finally, our results in mouse embryonic fibroblasts indicate that Kif3a inactivation in the absence of any Pax8 gene alteration leads to altered GPCR plasma membrane expression, as shown for the β2 adrenergic receptor, and we suggest that a similar mechanism may explain the altered TSH signaling and mild hypothyroidism detected in Kif3a∆/flox Pax8Cre/+ mutant mice. [less ▲] Detailed reference viewed: 5 (3 ULg)Demixing processes in AgPd superlattices; Verstraete, Matthieu  ; et alin Journal of Physics : Condensed Matter (2009), 21 Detailed reference viewed: 15 (3 ULg) Epitaxial growth of CdTe on Si by Molecular Beam EpitaxySamain, Louise ; ; et alin Physicalia Magazine (2008), 30(3), 99-112 Detailed reference viewed: 9 (1 ULg)Atomic structure of the Te/Si(100)-2x1 surface; ; et al in Physical Review. B : Condensed Matter (2005), 72 Detailed reference viewed: 8 (3 ULg)Electronic structure of Ag-Pd heterostructuresVerstraete, Matthieu ; ; et alin Computational Materials Science (2004), 30 Detailed reference viewed: 17 (13 ULg)High-Yield Synthesis Of The Bovine Leukemia-Virus (Blv) P24 Major Internal Protein In Saccharomyces-Cerevisiae; ; Portetelle, Daniel et alin Gene (1989), 79(2), Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a ... [more ▼] Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a yeast-E. coli shuttle vector carrying the PHO5 promoter, the p24 gene and the CYC1 transcription terminator. After low inorganic phosphate (Pi) induction of the PHO5 promoter, p24 accumulated in the producing cells up to a concentration representing 10% of total soluble proteins. The expression level of p24 gene was not increased by insertion of the positive regulatory gene PHO4 on the p24 expression vector. The p24 produced in this system and incubated in crude yeast extract showed a remarkably high resistance to proteolytic degradation, a feature that presumably correlates with the compact globular conformation of the protein combined to the stabilizing effect of the N-terminal residue. [less ▲] Detailed reference viewed: 7 (0 ULg)Biochemical And Immunological Characterization Of The Bovine Leukemia-Virus (Blv) Envelope Glycoprotein (Gp51) Produced In Saccharomyces-Cerevisiae; Portetelle, Daniel ; et alin Gene (1989), 79(2), Detailed reference viewed: 5 (1 ULg)Expression of the gene coding for the bovine leukemia virus (BLV) major internal protein p24 in the yeast Saccharomyces cerevisiae.; ; Portetelle, Daniel et alin Yeast (Chichester, England) (1988), 4 Detailed reference viewed: 2 (0 ULg) |
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