References of "Dumas, Dominique"
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See detailEffet des antibiotiques sur le procédé de traitement des eaux par boues activées ; étude à l’échelle du pilote et du floc par microscopie 3D
Potier, Olivier; Louvet, Jean-Noël ULg; Pons, Marie-Noëlle et al

Conference (2012, February 02)

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See detailEffet de l'érythromycine sur le traitement par boues activées
Louvet, Jean-Noël ULg; Giammarino, Cinzia; Dumas, Dominique et al

Poster (2012)

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See detailTracer application at different scales for wastewater treatment: from bacteria to industrial plant
Potier, Olivier; Louvet, Jean-Noël ULg; Le Moullec, Yann et al

Conference (2011, June)

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See detailHemocompatibility and biofunctionality of two poly(2- (dimethylamino)ethyl methacrylate-co-poly(ethyleneglycol) copolymers
Riquelme, Bibiana D.; Dumas, Dominique; Fontana, Alicia et al

in Journal of Biomedical Materials Research (2011), 99A (3)

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See detailSimultaneous Gram and viability staining on activated sludge exposed to erythromycin: 3D CLSM time-lapse imaging of bacterial disintegration
Louvet, Jean-Noël ULg; Attik, Ghania; Dumas, Dominique et al

in International Journal of Hygiene and Environmental Health (2011), 214

The effect of erythromycin on activated sludge bacteria according to their Gram type was investigated with 3-dimensional Confocal Laser Scanning Microscopy (CLSM) time-lapse imaging. The fluorescent stains ... [more ▼]

The effect of erythromycin on activated sludge bacteria according to their Gram type was investigated with 3-dimensional Confocal Laser Scanning Microscopy (CLSM) time-lapse imaging. The fluorescent stains SYTOX Green and Texas Red-X conjugate of wheat germ agglutinin stained dying bacteria and Gram + bacteria respectively. Time-lapse imaging allowed an understanding of the staining mechanism and the measurement of the death rate. In presence of erythromycin (10 mg/L), Gram + bacteria had a higher mortality rate than the Gram − bacteria. This result suggests that antibiotic in wastewater could change the activated sludge bacteria composition, according to their Gram type by selecting the bacteria which are the least sensitive to the antibiotics. However bacterial death was followed by bacterial disintegration leading to a decrease in the fluorescence. Results suggested that the viability indicators based on membrane integrity should be used with a correct sampling method, which can give the initial quantity of living bacteria. [less ▲]

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See detailL’imagerie Confocale : un Outil Performant pour le Génie des Procédés Biologiques
Louvet, Jean-Noël ULg; Attik, Ghania; Hupont, Sébastien et al

Poster (2010)

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See detailWastewater treatment: effect of antibiotics on removal efficiency and a new approach to modeling transport, transfer, and reactions
Potier, Olivier; Louvet, Jean-Noël ULg; Le Moullec, Yann et al

Scientific conference (2010)

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See detailAdverse effects of antibiotics on activated sludge process: the erythromycin case
Louvet, Jean-Noël ULg; Attik, Ghania; Dumas, Dominique et al

Conference (2010)

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See detailAssessment of erythromycin toxicity on activated sludge via batch experiments and microscopic techniques (epifluorescence and CLSM)
Louvet, Jean-Noël ULg; Helouin, Yannick; Attik, Ghania et al

in Process Biochemistry (2010), 45

This study investigates erythromycin toxicity toward activated sludge as a function of exposure time and antibiotic concentration. Batch experiments were conducted and microscopic techniques ranging from ... [more ▼]

This study investigates erythromycin toxicity toward activated sludge as a function of exposure time and antibiotic concentration. Batch experiments were conducted and microscopic techniques ranging from bright-field microscopy to epifluorescence and confocal laser scanning microscopy (CLSM), combined with a fluorescent viability indicator (BacLight ® Bacterial Viability Kit, Molecular Probes), allowed us to study erythromycin time-kill activity. The erythromycin toxicity was observed at lower concentration when exposure time increased. A 4 .g/L erythromycin concentration was toxic to heterotrophic bacteria on a 5-day time exposure, and a 5 mg/L concentration inhibited nitrification. These findings are in agreement with the microscopic studies, which showed a latency time before the lower antibiotic concentrations began to kill bacteria. Microscope slide wells were used as micro-reactors in which erythromycin concentration ranged from 0.1 to 1 mg/L. After 45 min there were 94% (SD 3.8) of living bacteria in control micro-reactors, 67% (SD 3.1) in micro-reactors that contained 0.1 mg/L erythromycin and 37% (SD 18.6) in micro-reactors that contained 1 mg/L erythromycin. CLSM allowed visualization of isolated stained cells in the three-dimensional structure of damaged flocs [less ▲]

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See detailImpact of antibiotics in wastewater on activated sludge treatment. The Erythromycin case
Louvet, Jean-Noël ULg; Potier, Olivier; Dumas, Dominique et al

Conference (2009, October 15)

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See detailConséquences négatives de la présence d'antibiotiques dans les eaux usées lors de leur épuration par boues activées
Louvet, Jean-Noël ULg; Attik, Ghania; Dumas, Dominique et al

Conference (2009)

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