References of "Dubois Dauphin, Robin"
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See detailLes entérocoques : avantages et inconvénients en biotechnologie (synthèse bibliographique)
Aguilar Galvez, A.; Dubois Dauphin, Robin ULg; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(1), 67-76

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See detailIn vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture
Dubois Dauphin, Robin ULg; vandeplas, Sabrina; Didderen, Isabelle et al

in Journal of Microbiology and Biotechnology (2011), 21(1), 62-70

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both ... [more ▼]

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both techniques. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, showed an anti-campylobacter activity in co-culture assay in using dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the complementation with Cellulase A complex (Beldem S.A.) of this medium enhanced antimicrobial effect of both bacteria. The co-culture medium acidification was correlated with the concentration in supplemented enzyme. The antibacterial effect was characterized by the production of lactic acid by the homofermentative E. faecium THT and the lactic and acetic acids production by the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties from bacteria-enzyme cooperation could reduce the prevalence of Campylobacter consequently the risk of human infection. [less ▲]

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See detailGenetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431
Aguilar Galvez, Ana Consuelo; Dubois Dauphin, Robin ULg; Campos Gutierrez, David Carlos et al

in Food Science and Biotechnology (2011), 20(2), 289-296

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanalproduced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and ... [more ▼]

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanalproduced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kb HindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii. [less ▲]

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See detailThe influence of growth conditions on enterocin-like production by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 isolates from artisanal Peruvian cheeses
Aguilar Galvez, Ana Consuelo ULg; Guillermo Arciniegas, Sandra Denisse; Dubois Dauphin, Robin ULg et al

in Annals of Microbiology (2011), 61

Strains of Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431, isolated from artisanal-produced Peruvian cheeses, offer a broad potential application as food biopreservatives for heat ... [more ▼]

Strains of Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431, isolated from artisanal-produced Peruvian cheeses, offer a broad potential application as food biopreservatives for heat-processing treatments. The aim of this work was to optimize the production conditions and medium composition for the production of bacteriocin-like substances (BLIS). The kinetics of the BLIS production for both strains is a growth-associated process, and the maximum inhibitory activity for E. faecium CWBI-B1430 and E. mundtii CWBI-B1431 was achieved after 9 and 8 h of fermentation, respectively. Temperature, initial pH, oxygen level, and their interactions all influence BLIS production for both strains; this influence was specific for each strain. The bacteriocin gene expression in a 20.0-L fermentor confirms previous work carried out by fermentation in the flask; the first reports of expression revealed mundticin KS from E. faecium and enterocin P from E. mundtii. Both strains demonstrated antilisteria activity and were multi-producers of enterocin. Furthermore, the study with whey milk as an inexpensive source of fermentable carbohydrates indicated lower BLIS production than that obtained with M17LG broth. The short chain fatty acid (SCFA) analyses offer a new alternative use for both strains, as strains probiotic, for propionic acid production. [less ▲]

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See detailTechniques de séchage des starters lactiques et mécanismes affectant la viabilité cellulaire suite à la lyophilisation
Coulibaly, Ibourahema ULg; Dubois Dauphin, Robin ULg; Danthine, Sabine ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 287-299

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See detailIsolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Bayane, Ali; Diawara, B.; Dubois Dauphin, Robin ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

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See detailSalmonella in chicken : Current and developing strategies to reduce contamination at farm level
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

in Journal of Food Protection (2010), 73(4), 774-785

Salmonella sp. is a human pathogen that frequently infects poultry flocks. Consuming raw or undercooked contaminated poultry products can induce acute gastro-enteritis in human. Faced with the public ... [more ▼]

Salmonella sp. is a human pathogen that frequently infects poultry flocks. Consuming raw or undercooked contaminated poultry products can induce acute gastro-enteritis in human. Faced with the public health concern associated with salmonellosis, the European Union (EU) has established a European regulation forcing Member States (MS) to implement control programs aimed at reducing Salmonella prevalence in poultry production, especially at the primary production level. The purpose of the present review article is to summarise the current research and to suggest future developments in the area of Salmonella control in poultry, which may be of value to the industry in the coming years. The review will focus especially on preventive strategies that have been developed and that aim at reducing the incidence of Salmonella colonization in broiler chickens at farm level. Besides the usual preventive hygienic measures, different strategies have been investigated, like feed and drinking water acidification by organic acids and immune strategies based on passive and active immunity. Modulating the diet in terms of ingredient and nutrient composition with the intent of reducing the bird’s susceptibility to Salmonella has also been examined. Because in-ovo feeding has been shown to accelerate small intestinal development and to enhance the epithelial cell function, it could also be an efficient tool to control enteric pathogens. Microflora-modulating feed additives, like antibiotics, prebiotics, probiotics, and synbiotics, represent another field of investigation, whose success depends on the additive used. Finally, recent control methods, such as chlorate products and bacteriophages, have also been studied. [less ▲]

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See detailPrevalence and sources of Campylobacter spp. contamination in free-range broiler production in the southern part of Belgium
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Palm, Rodolphe ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(2), 279-288

A one year epidemiological study was carried out between February 2005 and January 2006 in the southern part of Belgium to assess the Campylobacter prevalence in free-range broiler production. Three ... [more ▼]

A one year epidemiological study was carried out between February 2005 and January 2006 in the southern part of Belgium to assess the Campylobacter prevalence in free-range broiler production. Three successive broiler flocks from six Belgian farms were investigated for the presence of Campylobacter spp. during the rearing period. Each flock was visited four times, before and after the outdoor rearing period. During each visit, samples were taken in the broiler house (litter, cecal droppings, water-lines, feed, anteroom) as well as from the outer rearing environment (open-air range). The Campylobacter detection in all samples was carried out according to the ISO 10272 standard. Identification was based on colonial morphology, microscopic examination, and biochemical tests. PCR multiplex was used for genetic confirmation. Campylobacter jejuni was the main species isolated from all contaminated samples. Overall, mixed infections C. jejuni/C. coli represented 40.6%, while C. jejuni and C. coli represented 46.9% and 12.5% of chicken samples respectively. A 100% flock contamination was observed in the 6 farms during the summer/autumn period, whereas only 66.7% and 33.3% of the flocks became Campylobacter-positive in spring and winter respectively, at the end of the rearing period. Half of contaminated flocks were infected before chickens have access to the open-air range. Environmental samples, especially the open-air range soil, were found to be Campylobacter-positive before flock infection. The other potential sources of contamination were delivery tray, anteroom floor and water-lines. Other animal productions like cattle on the farm, no applied rodent control, no cleaning and disinfection of water-lines between flocks, no detergent used for cleansing and thinning were recorded as risk factors. In conclusion, the contact with the environment, particularly the access to an open-air range, appeared to be the major way of Campylobacter contamination of chickens in free-range broiler production. [less ▲]

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See detailEffect of the bacterial or fungal origin of exogenous xylanases supplemented to a wheat-based diet on performance of broiler chickens and nutrient digestibility of the diet
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Thonart, Philippe ULg et al

in Canadian Journal of Animal Science (2010), 90(2), 221-228

Two identical experiments were carried out to study the effects of four xylanases from bacterial or fungal origin supplemented to a wheat-based diet, on growth performance of broiler chickens and nutrient ... [more ▼]

Two identical experiments were carried out to study the effects of four xylanases from bacterial or fungal origin supplemented to a wheat-based diet, on growth performance of broiler chickens and nutrient digestibilities. Experimental treatments consisted of a control basal diet containing 600 g kg-1 wheat (C), and the basal diet supplemented with 0.1 g kg-1 Grindazyme G from Aspergillus niger (G), 0.1 g kg-1 Belfeed B1100MP from Bacillus subtilis (B), 0.1 g kg-1 Roxazyme G from Trichoderma viride (R), or 0.0125 g kg-1 of a xylanase from Aspergillus aculeatus (A). Each experimental diet was given to four groups of six chickens each. Growth performance and feed conversion ratio (FCR) were recorded weekly, from 7 to 21 d of age. In the second experiment, a digestion balance trial was performed from 27 to 31 d of age to evaluate the nitrogen-corrected apparent metabolizable energy (AMEn) and the digestibilities of nitrogen, crude fat, starch and crude fibre. From 7 to 21 d of age, xylanase supplementation led to increased final body weight and daily weight gain, by 3.7 and 4.5 % (P < 0.05), respectively, without significant difference according to the xylanase origin. Xylanase supplementation significantly increased the AMEn (+2.6 %), and the digestibilities of crude fibre (+58.9 %) and nitrogen (+1.6 %). Increase in AMEn as well as in crude fat and starch digestibilities were significantly different according to the xylanase, but were not dependent on fungal or bacterial origin. In conclusion, the microbial origin of xylanases supplemented to wheat-based diets influenced neither the performance of broiler chickens nor the improvement in nutrient digestibilities [less ▲]

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See detailPeroxisomal β-oxidation and Production of γ-decalactone by the Yeast Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Dubois Dauphin, Robin ULg; Aguedo, Mario ULg et al

in Notulae Botanicae Horti Agrobotanici Cluj-Napoca (2010), 2

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See detailPlasmid-associated bacteriocin production by Lactobacillus LMG21688 Listeria monocytogenes growth rebound in a food system.
Kouakou, P.; Dortu, C.; Dubois Dauphin, Robin ULg et al

in FEMS Microbiology Letters (2010), 306(1), 37-44

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See detailEfficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Thiry, Christophe ULg et al

in Poultry Science (2009), 88(8), 1643-1654

Three experiments were performed to assess the ability of a Lactobacillus plantarum probiotic combined with a xylanase to reduce the effects of S. typhimurium infection in broiler chickens from 1- to 30 ... [more ▼]

Three experiments were performed to assess the ability of a Lactobacillus plantarum probiotic combined with a xylanase to reduce the effects of S. typhimurium infection in broiler chickens from 1- to 30- or 42-d-old. Chicks were challenged at 3-d-old with 108 or 105 cfu S. typhimurium/chick. Four diets were studied: a wheat-based diet (C+) supplemented with 0.1 g/kg xylanase (E), or 106 cfu/g or L. plantarum (P), or both (PE). Uninfected chicks fed the C diet were used as negative control (C-). Six or 8 chicks were housed per cage with 9 cages/treatment. Growth performance and feed conversion ratio (FCR) were recorded weekly. In experiment 1, bacterial enumeration in caeca was achieved using the fluorescent in situ hybridization technique. Salmonella enumeration was realized in excreta by microbiological cultures (Exp. 2 and 3). Nutrient digestibilities and AMEn were determined in experiment 3 from d 35 to d 39. Infection with S. typhimurium led to a significant decrease in the daily weight gain (DWG) by 23.6% to 32.8%, whereas FCR was increased by 1.0% to 19.7%. Chickens fed the PE diet showed significantly improved performance in comparison with C+ birds (DWG: +12.5% in Exp. 1; FCR: -2.1-8.6%), and in comparison with the P and E treatments (DWG: +6.3-8.3% in Exp. 1; FCR: -2.7-6.4%). In experiment 3, the FCR was significantly improved by 3% with the PE diet in comparison with C- chickens. The PE combination tended to restore a microflora similar to that of uninfected broilers, whereas the P and E diets had less of an effect on the profile of bacterial communities. At slaughter age, Salmonella contamination was reduced by 2.00 and 1.85 log cfu for the E and PE treatment, respectively. The PE diet significantly reduced the crude fat digestibility by 9.2%, in comparison with the C+ chickens. These results suggest that combination between L. plantarum and a xylanase as feed additive could be effective for reduction of detrimental effect following S. typhimurium infection of broilers. [less ▲]

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See detailDetermination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage.
Coulibaly, Ibourahema ULg; Yao, Amenan Anastasie ULg; Dubois Dauphin, Robin ULg et al

Poster (2009, April 01)

The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs ... [more ▼]

The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs), sterol ester (SEs), triglycerides (TGs), diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. . PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during stored at 20°C and 40°C with or without lithothamne400®, respectively. [less ▲]

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See detailDescription of two Enterococcus strains isolated from traditional Peruvian artisanal-produced cheeses with a bacteriocin-like inhibitory activity
Aguilar Galvez, Ana Consuelo ULg; Dubois Dauphin, Robin ULg; Ghalfi, Hakim et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(3), 349-356

The aim of this work was to isolate and to characterize strains of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity from 27 traditional cheeses artisanal-produced obtained from ... [more ▼]

The aim of this work was to isolate and to characterize strains of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity from 27 traditional cheeses artisanal-produced obtained from different Peruvian regions. Twenty Gram+ and catalasenegative strains among 2,277 isolates exhibited bacteriocin-like inhibitory activity against Listeria monocytogenes CWBIB2232 as target strain. No change in inhibitory activity was observed after organic acid neutralization and treatment with catalase of the cell-free supernatant (CFS). The proteinic nature of the antimicrobial activity was confirmed for the twenty LAB strains by proteolytic digestion of the CFS. Two strains, CWBI-B1431 and CWBI-B1430, with the best antimicrobial activity were selected for further researches. These strains were taxonomically identified by phenotypic and genotypic analyses as Enterococcus mundtii (CWBI-B1431) and Enterococcus faecium (CWBI-B1430). The two strains were sensitive to vancomycin (MIC < 2 μg.ml-1) and showed absence of haemolysis. [less ▲]

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See detailThe role of the specific immune response on cecal colonization of slow-growing broiler chickens reared on litter contaminated by Campylobacter jejuni
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

Conference (2009)

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11 ... [more ▼]

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11-weeks experiment was carried out in a 45 m2 broiler house with 500 chickens from 1 day of age, which had access to an open-air range. Litter contamination was performed before the setting up of the chicks by rearing campylobacter-positive cocks. These birds were also used to contaminate the open-air range during one week before the exit of the chickens, at 5 weeks of age. Blood samples were taken weekly on 20 chickens for anti-campylobacter IgG quantitative measurements on serum. Chickens were then euthanized and cecal contents and bile were collected for Campylobacter enumeration and anti-campylobacter IgA quantification, respectively. A high serum anti-campylobacter IgG titer of 110.13 was measured in one-day-old chicks. The IgG antibodies level significantly decreased to 5.60 at 28 days of age which coincided with the onset of Campylobacter infection in the flock. A significant reduction of the cecal colonization from 11 weeks of age was correlated with the increase in biliary IgA titers. These results suggest that the 2-3 week’s delay generally observed in poultry production before flock infection by Campylobacter may be, at least partly, explained by the protective effect of maternal antibodies. The increase of secretory immune response as bird age may help to limit Campylobacter population in the chicken gut. [less ▲]

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See detailEffects of curing sodium nitrite additive and natural meat fat on growth control of Listeria monocytogenes by the bacteriocin-producing Lactobacillus curvatus strain CWBI-B28.
Kouakou, P.; Ghalfi, H.; Destain, Jacqueline ULg et al

in Food Microbiology (2009), 26

In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In ... [more ▼]

In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 C with bacteriocin-producing CWBIB28 in lean pork meat (fat content: 13%) without added nitrite, a strong antilisterial effect was observed after one week. The effect was maintained for an additional week, after which a slight and very gradual rebound was observed. Both added nitrite (20 ppm) and a high-fat content (43%) were found to antagonise this antilisterial effect, the Listeria cfu count reached after six weeks being 200 times as high in high-fat meat with added nitrite than in lean meat without nitrite. This antagonism could not be attributed to slower growth of the bacteriocin-producing strain, since CWBI-B28 grew optimally in fatrich meat with 20 ppm sodium nitrite. Bacteriocin activity was also measured in the samples. The observed activity levels are discussed in relation to the degree of antilisterial protection conferred [less ▲]

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See detailLa conservation du poisson au Senegal : utilisation d’une souche locale de Lactococcus lactis
Diop, Michel; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Cahiers Agricultures (2009), 18(4), 337-342

L’effet antimicrobien du surnageant de culture neutralisé (SCN) bactéricide issu de Lactococcus lactis CWBI-B1410 d’origine locale, utilisé seul ou en combinaison avec du chlorure de sodium (NaCl) comme ... [more ▼]

L’effet antimicrobien du surnageant de culture neutralisé (SCN) bactéricide issu de Lactococcus lactis CWBI-B1410 d’origine locale, utilisé seul ou en combinaison avec du chlorure de sodium (NaCl) comme conservateurs, a été évalué sur poissons maigre (Pomadasys jubelini), moyennement gras (Polydactylus quadrifilis) et gras (Arius heudeloti)] au Sénégal. Les poissons ont été achetés dans un marché local, éviscérés, lavés avec de l’eau de robinet potable, puis filetés. Cent millilitres de SCN de CWBI-B1410 non salé et salé ont été additionnés dans 100 grammes de filets (concentration finale de NaCl entre 0 et 7 %) dans des bocaux en verre conservés à 10 °C. L’évolution de la flore mésophile totale (FMT) des filets a été comparée à celles de filets traités avec du SCN non salé et salé (concentration finale de NaCl entre 0 et 7 %), issu de L. lactis LMG6890 ne produisant pas de bactériocine. Un niveau de FMT de 106 micro-organismes par gramme (ufc/g) a été considéré comme la fin de la durée de conservation. Le niveau de la flore mésophile totale des filets crus atteignait 5,74 log ufc/g. L’ajout de SCN de CWBI-B1410 dans les filets de poisson maigre réduit la flore mésophile totale de 1 log ufc/g et stabilise la flore pendant 4 jours – correspondant à la durée de conservation de ces filets a` 10 °C, contre 0,5 jour pour le contrôle négatif. L’addition de SCN de CWBIB1410 salé sur les poissons (NaCl, 7 %) réduit davantage la FMT et retarde sa croissance à 10°C, entraînant comme résultat l’augmentation de la durée de conservation de respectivement 12, 7,5 et 8 jours pour le poisson maigre, moyennement gras et gras. Ces résultats suggèrent que cette stratégie peut constituer un moyen convenable d’améliorer la conservation des produits halieutiques tropicaux. [less ▲]

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See detailUse of a Nisin-Producing Starter Culture of Lactococcus lactis subsp. lactis To Improve Traditional Fish Fermentation in Senegal
Diop, Michel; Dubois Dauphin, Robin ULg; Destain, Jacqueline ULg et al

in Journal of Food Protection (2009), 72(9), 1930-1934

Lactococcus lactis subsp. lactis strain CWBI B1410, which produces various antibacterial compounds including organic acids and nisin, was used as a starter culture to improve the traditional Senegalese ... [more ▼]

Lactococcus lactis subsp. lactis strain CWBI B1410, which produces various antibacterial compounds including organic acids and nisin, was used as a starter culture to improve the traditional Senegalese fish fermentation in which fish are mostly transformed to guedj by spontaneous fermentation for 24 to 48 h at ambient temperatures near 30uC followed by salting (with NaCl) and sun drying. Assays were performed on lean fish (Podamasys jubelini) and fat fish (Arius heudelotii) purchased at a local market. The total viable microbial counts in raw fillets of P. jubelini and A. heudelotii were 5.78 and 5.39 log CFU/g, respectively. Populations of enteric bacteria (which can include pathogenic bacteria) in P. jubelini and A. heudelotii were 4.08 and 4.12 log CFU/g, respectively. Spontaneous fermentation of raw fillets at 30uC led to the proliferation of enteric bacteria to 9 log CFU/g after 24 h in fermented P. jubelini and A. heudelotii fillets with pH values of 6.83 and 7.50, respectively. When raw fish fillets were supplemented with glucose (1%, wt/wt) and inoculated with Lactococcus lactis (107 CFU/g), the pH decreased to about 4.60 after 10 h at 30uC, and nisin activity was detected in juice from the fillets. Traditionally fermented fillets of P. jubelini and A. heudelotii contained enteric bacteria at higher levels of 4 and 2 log CFU/g, respectively, than did fillets of the same fish supplemented with glucose and fermented with the starter culture. These data suggest that this new fish fermentation strategy combined with salting and drying can be used to enhance the safety of guedj [less ▲]

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See detailCharacterization of lactic acid bacteria isolated from poultry farms in Senegal
Coulibaly, Ibourahema ULg; Dubois Dauphin, Robin ULg; Destain, Jacqueline ULg et al

in African Journal of Biotechnology (2008), 7(12), 2006-2012

The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known and these organisms have been characterized extensively by using different techniques. In this study ... [more ▼]

The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known and these organisms have been characterized extensively by using different techniques. In this study, thirty lactic acid bacterial strains were isolated from soils chicken faeces and feathers. A total of nineteen isolates were obtained and by sequential screening for catalase activity and Gram-staining, eight were determined to be LAB out of which six were established to be homofermentative by the gel plug test. Five isolates were identified by use of the API 50CHL kit and four Lactobacilli strains and one Lactococci strain were selected to study their growth and lactic acid production profiles in a time course experiment. The Lactobacilli strains, both isolated from faeces, produced higher amounts of cells and lactic acid from soils as compared to the lactococci strain isolated from feathers. L (+)-lactic acid is the only optical isomer for use in pharmaceutical and food industries because is only adapted to assimilate this form. The optical isomers of lactic acid were examined by L (+) and D (-) lactate dehydrogenase kit. Lactobacilli strains produced combination of both optical isomers of lactic acid. Among them, Lactobacillus casei subsp. casei produced the low amount of D (-)-lactic (2%). The optimum rates of glucose for lactic acid production by Lactobacillus strains were 180 and 120 g/l for Lactobacillus plantarum and Lactobacillus paraplantarum, respectively. [less ▲]

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