References of "Dezelee, P"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailThe Cell Wall Peptidoglycan of Bacillus megaterium KM. I. Studies on the Stereochemistry of α,α'-Diaminopimelic Acid
Bricas, E.; Ghuysen, Jean-Marie ULg; Dezelee, P.

in Biochemistry (1967), 6(8), 2598-2607

α,α'-Diaminopimelic acid (DAP) occurs in the wall peptidoglycan of Bacillus megaterium KM predominantly in the form of its meso isomer (about 85% of the total residues) and, in minor amounts, in the form ... [more ▼]

α,α'-Diaminopimelic acid (DAP) occurs in the wall peptidoglycan of Bacillus megaterium KM predominantly in the form of its meso isomer (about 85% of the total residues) and, in minor amounts, in the form of its DD isomer. The amino groups on the L carbon of the meso-DAP residues are involved in peptide linkages to the glutamic acid residues. Most of the amino groups on the D carbon of the meso-DAP residues are free; some of them are substituted, thus probably serving to cross-link peptide subunits. These amino groups can be liberated by a Streptomyces endopeptidase. None of the DD-DAP residues have amino groups free. Moreover, these groups are not liberated by endopeptidase treatment. The peptidoglycan upon enzymatic degradation yields mainly two fractions. A major fraction is composed of disaccharide peptide monomer subunits containing only the meso isomer of DAP. A second minor fraction is composed of disaccharide peptide oligomers containing both meso and DD isomers of DAP. The meso-DAP residues isolated as monodinitrophenyl derivatives from both fractions have optical rotations and optical rotatory dispersions identical with that of synthetic monodinitrophenyl-meso-DAP obtained by dinitrophenylation of the amino group on the D carbon. The assignment of the DD configuration to the DAP residues which are not meso rests upon the optical rotatory properties of their bisdinitrophenyl derivatives. [less ▲]

Detailed reference viewed: 25 (2 ULg)